Species richness and composition pattern of amphibians along elevation gradients in eastern Nepal Himalaya are rarely investigated. This is a first ever study in the Himalayan elevation gradient, the world’s highest mountain range and are highly sensitive to the effects of recent global changes. The aim of the present study was to assess amphibian community structure along elevation gradients and identify the potential drivers that regulate community structures. Amphibian assemblages were sampled within 3 months in both 2014 and 2015 (from May to July) using nocturnal time constrained and acoustic aids visual encounter surveys. In total, 79 transects between 78 and 4200 m asl were sampled within 2 years field work. A combination of polynomial regression, generalized linear models, hierarchical partitioning and canonical correspondence analysis were used to determine the effects of elevation and environmental variables on species richness, abundance, and composition of amphibian communities. Species richness and abundance declined linearly with increasing elevation, which did not support the Mid-Domain Model. Among all the environmental variables, elevation, surface area and humidity were the best predictors of species richness, abundance and composition of amphibians. The majority of amphibian species had narrow elevation ranges. There was no significant correlation between species range size and elevation gradients. However, body size significantly increased along elevation gradients, indicating that Bergmann’s rule is valid for amphibians in eastern Nepal Himalaya. This study indicates that eastern Nepal Himalaya is a hotspot in amphibian diversity, and it should be served as a baseline for management and conservation activities.

The toad genus Oreolalax is widely distributed in southwest China and northern Vietnam. A new species of the genus is described from Sichuan Province, China. Phylogenetic analyses based on the mitochondrial 12S rRNA and 16S rRNA gene sequences supported the new species as an independent clade clustered into the clade also containing O. nanjiangensis and O. chuanbeiensis. The new species can be distinguished from its congeners by a combination of the following characters: body size moderate (SVL 51.2–64.2 mm in males); head broad; tympanum hidden; interorbital region with dark triangular pattern; belly with marbling; male lacking spines on lip margin; spiny patches on chest small with thick sparse spines in male; nuptial spines thick and sparse; tibio-tarsal articulation reaching beyond nostril when leg stretched forward; toe webbing at base.

Declining body size is a universal ecological response to globalwarming in ectotherms. Ectotherms growfaster but mature at a smaller size at higher temperatures. This phenomenon is known as the temperature-size rule (TSR). However, we know little about the details of the relationship between temperature and size. Here, this issue was studied in the Chinese giant salamander (Andrias davidianus), one of the largest extant amphibians and a flagship species of conservation in China. Warm-acclimated A. davidianus larvae (25 degrees C) had accelerated development but little superiority in body growth when compared to their 15 degrees C counterparts when fed with red worm. This predicts a drastic decrease in adult body size with warming. However, a fish diet (more abundant lipid and protein) improved the growth performance at 25 degrees C. The underlying mechanism was studied. Warm-acclimated larvae had enlarged livers but shortened tails (fat depot). Their livers suffered fromenergy deficiencies and decreased protein levels, even when protein synthesis and energy metabolismwere transcriptionally upregulated. This could be a direct explanation for their poor growth performance. Further analyses revealed a metabolic disorder resembling mammal glycogen storage disease in warm-acclimated larvae, indicating deficiency in glycogen catabolism. This speculation is consistent with their increased lipid and amino acid catabolism and explained the poor energy conditions of the warm-acclimated larvae. Additionally, a deficiency in glycogen metabolism explains the different efficiency of worm and fish diets in supporting the growth of warm-acclimated larvae, even when both diets were provided sufficiently. In conclusion, our results suggest that the relationship between temperature and body size can be flexible, which is a significant finding in terms of the TSR. The underlying metabolic and nutrient mechanisms were revealed. This knowledge can help deepen our understanding of the consequences of warming and can contribute to the conservation of A. davidianus.