The biodeterioration of wooden cultural heritage is a severe problem worldwide and fungi are the main deteriorating agents. The identification of effective natural products, safer for humans and the environment, is a current challenge. Ten deteriorated archaeological objects (a wooden statue of a seated man, an anthropoid wooden coffin with a cartonnage mummy of Nespathettawi, and a wooden box of Padimen’s son), stored at the Egyptian museum in Cairo, were considered here. The wood species of the three most deteriorated objects were previously identified as Acacia nilotica, Ficus sycomorus, and Tamarix gennessarensis. Twenty-six fungal species were isolated and identified from the wooden objects and the four most frequent species belonged to the genus Aspergillus. Fourteen fungal species among those isolated showed the greatest biodeterioration activity on the experimental wood blocks of the archaeological objects. The antifungal activities of several eco-friendly plant essential oils (from cinnamon, eucalyptus, frankincense, geranium, lavender, lemongrass, menthe, rosemary, tea tree, and thyme) and plant extracts (from basil, eucalyptus, henna, melia, and teak) were tested against the fungal species with the greatest biodeterioration activity. The essential oils (Eos) were more effective than the plant extracts. Thyme EO, followed by geranium and cinnamon ones, was the most active (minimum inhibitory concentrations: 0.25–1 µL/mL). These EO; also showed inhibitory effects on the enzymatic activities (cellulase, amylase, and protease) of the four most dominant fungal species. Thymol and p-cymene were the two main components of thyme oil, while geraniol and beta-citronellol were those of geranium oil; eugenol and caryophyllene were those of the cinnamon EO. Thyme oil applied to the most deteriorated experimental aged A. nilotica wooden cubes inoculated with the four highly frequent fungal species was effective in wood preservation. Moreover, no significant interference was observed in the wood before and after thyme treatment. Thyme oil seems to be a promising eco-friendly antifungal agent for the preservation of archaeological wooden artefacts.

This study presents a multi-analytical investigation of the wooden components from Khufu’s Second Solar Boat, one of the valuable archaeological discoveries in ancient Egypt. The research integrates advanced imaging and analytical techniques to identify wood species, assess deterioration patterns, and characterize the fungal and bacterial biodeteriogens. The initial visual examination documented the state of preservation at the time of discovery. Subsequently, the identification of the wood species was carried out. The deterioration status was assessed using a variety of tools, including scanning electron microscopy (SEM) and high-resolution synchrotron radiation computed microtomography (SR-μCT) for morphological alterations, X-ray diffraction (XRD) for crystallinity changes and Fourier-transform infrared spectroscopy (FTIR) to assess chemical degradation indexes of wood. Moreover, molecular techniques were used to identify and characterize the presence of biodeterioration agents. Results indicate that ancient craftsmen used cedar wood for the boat construction. The analysed samples exhibited advanced biotic and abiotic degradation, as evidenced by microbiological assessments, XRD measurements of cellulose crystallinity, FTIR indices, SEM micrographs and SR-μCT data. These results provide crucial insights into the long-term degradation processes of archaeological wood in arid environments, enhancing our understanding of ancient Egyptian woodworking practices and informing future conservation strategies for similar artifacts.

A recently discovered Egyptian wooden statue of King Djedefre was studied together with some surrounding burial soil samples for assessing the statue biodeterioration. The wooden morphological characterisation identified the hardwood Acacia nilotica as the wood type. X-ray diffraction, micro-FT-IR spectroscopy, and scanning electron microscopy with an X-ray spectrometer were used to evaluate the wood deterioration degree and the soil contribution in wood biodeterioration. Microbiological analyses (fluorescent in situ hybridisation and polymerase chain reaction) were also performed to detect the microbial attack on the statue. The prolonged interaction of the statue with the burial environment caused a strong wood decay due to biotic (fungi and bacteria) and abiotic factors (e.g., humidity fluctuations of the burial environment), which caused the severe cracking and collapsing of the wood structures. The analyses of the burial soil mineral composition were relevant for obtaining an overall picture of the statue deterioration. The results are useful for planning the right conservation procedures for this very particular and important wooden statue. Furthermore, analysis of the woody cell wall will help in the selection of appropriate consolidation and recovery treatments. Because the statue is a unique single piece of wood, and the morphological observations indicated that it is a bald woman in a sitting position, this statue will provide new and interesting knowledge of Egyptian culture.

A non-invasive XRD-XRF portable system for Archaeometry based on Angle and Energy Dispersive XRD (Hybrid XRD) plus XRF has been previously proposed by the authors. It gathers an attractive compromise between analytical performance and measurement time, as well as a higher energy penetration with respect to conventional Angle Dispersive XRD (ADXRD). Penetration to inside layers, usually perceived as an inconvenient for non-invasive portable XRF or XRD analyzer, can instead be informative for the proposed hybrid XRD-XRF system to perform depth profiling analysis. This hybrid configuration harness from the redundancy of the data, in the sense that a single XRF or XRD specimen, taken at the same sample point, appears in multiple spectrograms to account for data counting statistics. On this work, the performance of the system on standard reference material, as well as the identification of pigments in the surface layer of paintings is evaluated. Specific Hybrid data processing was conceived to examine the layer structure of the painting, by distinguishing signal patterns of the exposed and the under layers. The system is ventured for the first time to the study of pictorial artworks in coffin and cartonnage samples from ancient Egyptian sarcophagi. Prussian blue pigment in a modeled painting is also discussed.

This study investigates a 26th Dynasty Ptah–Sokar–Osiris wooden statuette excavated from the Tari cemetery, Giza Pyramids area, to decode ancient Egyptian manufacturing techniques and establish evidence-based conservation strategies of such wooden objects. Using minimal sampling (1.0–2.0 mm²), integrated XRF, synchrotron-based X-ray diffraction, FTIR, and confocal microscopy distinguished original technological choices from burial-induced alterations. The 85 cm Vachellia nilotica sculpture exhibits moderate structural preservation (cellulose crystallinity index 62.9%) with partial chemical deterioration (carbonyl index 2.22). Complete pigment characterization identified carbon black, Egyptian Blue (cuprorivaite, 55 ± 5 wt %), atacamite-dominated green (65 ± 5 wt %) with residual malachite (10 ± 2 wt %), orpiment (60 ± 5 wt %), red ochre (hematite, 60% ± 5 wt %), white pigments (93 ± 5 wt % calcite), and metallic gold (40 ± 5 wt %). Confocal microscopy revealed sophisticated multi-pigment mixing strategies, with black carbon systematically blended with chromophores for nuanced color effects. Atacamite predominance over malachite provides evidence for chloride-mediated diagenetic transformation over 2600 years of burial. Consistent calcite detection (~ 20–45%) across colored layers confirms systematic ground layer application, establishing technological baseline data for 26th Dynasty Lower Egyptian workshops. Near-complete organic binder loss, severe lignin oxidation, and ongoing salt-mediated mineral transformations indicate urgent conservation needs requiring specialized consolidants, paint layer stabilization, and controlled environmental storage. This investigation demonstrates synchrotron methods’ advantages while establishing a minimally invasive framework for studying polychrome wooden artifacts.

The cavity-induced pits play a major role in fs-PLAL, and the cavity-induced periodicity is a unique phenomenon. Fine periodic features ( $\Lambda < \lambda $ /2) are visible both inside and outside the craters, according to experimental data obtained at various liquid highs. Pits are forced to self-organize due to the crowding and jostling of approximately 108 cavitation bubbles, which is the cause of the development of these patterns. Based on ablation at 100 KHz for 6 minutes and the assumption that each pulse has 5 cavities, an estimated 108 cavities can be found. The thermal effect as the mechanism of pit formation is strongly supported by the emergence of metallic droplets and heat-affected zones outside the crater. Within the crater, another periodic structure with a bigger periodicity is seen, which is explained by the laser’s incubation effect as a result of the confinement and cooling effects of water. It is surprising that basic, easily accessible technology can be used to identify the phenomenon.