Recently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and microfluidic chip have been conducted. In this study, a microfluidic chip embracing a nanofiber scaffold is presented. A electrospun nanofiber scaffold can provide 3D cell culture conditions to a microfluidic chip environment, and its perfusion method in the chip can allow real-time monitoring of cell status based on the conditioned culture medium. To justify the applicability of the developed chip to 3D cell culture and real-time monitoring, HepG2 cells were cultured in the chip for 14 days. Results demonstrated that the cells were successfully cultured with 3D culture-specific-morphology in the chip, and their albumin and alpha-fetoprotein production was monitored in real-time for 14 days.

Body temperature is an important indicator of the health status of the human body. Thus, numerous studies have been conducted in various fields to measure body temperature. In this study, a biocompatible thermochromic membrane that changes its color when the temperature becomes higher than the transition temperature for thermochromism was fabricated using an extrusion-based three-dimensional printing process. The printing material was prepared by mixing a thermochromic pigment and a thermoplastic polymer in various ratios. The effects of mixing ratio on the various properties of the fabricated membranes were experimentally investigated. It is presented that the fabricated lattice membrane had excellent thermochromic reaction, which was experimentally evaluated using a measurement of color brightness. The pigment content affected the diameter and surface morphology of the printed filament. The elastic modulus decreased, and thermochromic response became faster as the pigment concentration increased. Subsequently, a patch for fever detection was developed and then attached to the skin to demonstrate its color change according to body temperature. Results show that the fabricated thermochromic patch could be successfully applied to fever detection.

As various healthcare technologies such as regenerative medicine, precision medicine, and alternative approaches to animal testing develop, the interest in the use and application of nano- and microfibers is steadily increasing. In this study, the effect of parylene-C coating on electrospun fibers was investigated, and a pattern coating method was developed to expand the potential utilization of parylene-C-coated electrospun fibers. An SEM analysis demonstrated that parylene-C was successfully deposited on the electrospun fibers without any failure such as pinholes or air bubbles. Biocompatibility was investigated through cell tests, which indicated that the coated fibers were non-toxic and supported cell growth well. Tensile tests demonstrated a significant increase in the elastic modulus of the parylene-C-coated fibers, with it nearly quadrupling compared to the original PCL fibers, and the fracture strength almost doubled. At the same time, hydrophobicity was well maintained without any statistically significant changes. In particular, a non-adhesive magnet–metal masking was proposed in order to selectively coat the electrospun fibers with parylene-C with a specific pattern. Furthermore, it was presented that the magnet–metal mask-based coating electrospun nanofibers with parylene-C could be used in the fabrication of hybrid fibers composed of different diameters and materials.