People are continuously subject to various environmental and chemical pollutants originating from industrial and agricultural activities. Heavy metals can be identified as aseparate group of xenobiotics that pose a danger to human health. They affect almost all body systems, exerting toxic, allergic,carcinogenic, and gonadotropic effects. The toxicity of heavy metals to the body, their neutralization and elimination from the body depends on several factors, including nutritional status, as there are detoxification mechanisms in the human body, which require the intakeof certain nutritional compounds. The quality of nutrition directly affects the state of the body since the essentialcompounds are mostly obtained from the food. Consumption of nutritional compounds is a significant factor that determines human health, growth, development, physical and mental activities and promotes more effective recovery during illness. The use of knowledge on the intoxication mechanisms and the role of alimentary factors in the body detox contribute to a deeper understanding of the processes in the development of the ways to neutralize the negative effects of various toxic compounds. At the same time, a large number of articles and reviews are devoted to the study and analysis of the manifestation of toxic effects of heavy metal compounds, mechanisms of their transformation in the environment, as well as damaging effects on the body systems; articles devoted to the study of the neutralization of the poisoning effect of various xenobiotics are aimed at investigating the detoxification effect of finished dosage forms. The importance of examining how nutritional factors like proteins, probiotics, vitamins, and dietary fiber contribute to the body detoxification processes is significant. This review analyses literature data of scientists from Kazakhstan, far and near abroad on the mechanisms of toxicity of a wide range of heavy metals, as well as the role of key nutrients in the process of their detoxification.

The using of bioactive compounds received from natural raw as nutriceutics is aknowledged as well as strengthening, immunomodulatory, adaptogenic agents and in order to reduce the risk of emergence of various diseases. Ganoderma Lucidum or Reishi mushroom is known on east during centuries and has been used in different aims. Modern researches of Reishi mushroom show its high antioxidative potential. Our experiments have shown that extracts made from micelium and fruiting body of ganoderma reduce the intensity of lipoperoxidation in membranes and increase the resistance in vitro conditions.Micelium extract inhibited the level of products of lipoperoxidation up to 37,6% at concentration 10 mkg, 21,1% at 20 mkg, 14,9% at 50 mkg. At influence the same concentrations of ascorbic acid means of level of lipid peroxidation amount 74,3%, 60,4% and 36,4% - respectively, for vitamin E - Е-70,2%, 65,3%, 53,5% and 38%respectively. Antioxidative effect of fruit body’s extract significantly have shown at 10-50 mkg – level of LPO decreased on 80% from origin level, the increasing of concentration from 50 to 100 mkg leaded to practically total inhibition of formation LPO products. At concentrations lower than 10 mkg antioxidative effect of the testing agents didn’t differ significantly. The experiments revealed that the mycelium extracts' antioxidant properties exceed the well-known antioxidant agents such as vitamins E and C, while the extract of the fruit body has a substantially similar effect of ascorbic acid and alpha-tocopherolResults of study have shown that extracts of ganoderma possess pronounced property to stabilize the structure of cell membrane. Severity of membrane stabilizing effect of extracts depended on concentration. In concentration 0-200 mkg in samples incubated in the presence of mycelium and fruit body extracts the hemolysis decreased on 19,8% and 25% at 10 mkg, on 27% and 37% at 20 mkg, on 55% and 57,2% at 50 mkg, on 63% and 68% at 100 mkg, on 69% and 65% at 200 mkg respectively, whereas at the introducing in samples the ascorbic acid in analogical concentrations the hemolysis have descended on 10%, 14,5%, 25%, 40% and 44%, and at the cariing in α-tocopherol the hemolysis lowered on 5%, 22%, 32%, 40%, 45% and 50% respectively.The researches of total polyphenols and flavonoids in extracts showed, that in extracts abscent compounds, which are related to flavonoids, whereas have found the existence of another polyphenolic compounds.

Nutrition is one of the fundamental factors of human health. Today the use of biologically active food additives, enrichment of products with nutraceuticals, consumption of target foods is justified due to the increasing effect of stress along with unbalanced diet and lifestyle. The main resource of nutrients is plant foods and substances derived from them. The use of additional plant components in the manufacture of food products contributes to increasing its biological and nutritional value. Non-traditional plant species have a high potential along with traditional crops. A lot of research is aimed at identifying the positive effects of bioactive substances of plants on the body and the possibility of using them to expand the range of useful food products. We investigated the prebiotic properties of the underground part of plants from the Brassicaceae family. In the course of the study we applied traditional microbiological methods of research. As the results of the experiments showed, the plant extracts promoted the growth of lactobacilli, and the prebiotic activity was not inferior to the common preparations - lactulose. The obtained data will be used for further research for the development of technology for obtaining products with a positive effect on the normoflora of the intestine and implying the inclusion of prebiotic substances in the formulation.

Relevance: Integrating molecular biomarkers with rigorous quality control (QC) measures in laboratory settings is essential for enhancing early detection strategies and prognostic evaluation in cancer patients. Precision and QC in laboratory diagnostics of oncological diseases have become particularly significant in the widespread implementation of targeted and personalized therapy. The study aimed to review publications evaluating quality control in biomarker identification within molecular genetics laboratories, using ovarian cancer diagnostics as a case study.Methods: This systematic literature review conducted in the framework of this study revealed 220 records, leading to 165 unique publications, of which 24 full-text articles were included in this review. The study followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 (PRISMA 2020) guidelines. Results: All analyzed sources showed that Implementing QC, including calibration, internal controls, and proficiency testing provided by the College of American Pathologists (CAP), significantly reduces errors despite ongoing funding constraints. The European Molecular Quality Network (EMQN) and CAP jointly offer proficiency testing programs to evaluate laboratory performance globally, ensuring consistency and reliability in testing outcomes. Conclusion: Ensuring the accuracy and reliability of molecular diagnostic tests is critical in clinical settings, particularly for conditions such as ovarian cancer, where precise genetic analysis informs both diagnosis and treatment. Further advancements in early detection and personalized treatment can be achieved by integrating emerging technological innovations within robust QC framework, ultimately improving patient outcomes. Consequently, the establishment of standardized guidelines and standard operating procedures for molecular genetic testing, with a specific focus on the molecular genetic diagnosis of ovarian cancer, is imperative.

В данной статье проводится анализ основных вопросов обеспечения экономических методов управления экологическим правопорядком как экономических инструментов, используемых для регулирования природопользования и охраны окружающей среды. Авторы отмечают, что, несмотря на свои преимущества, экономические методы управления экологическим правопорядком имеют ряд проблем, которые необходимо решать для их эффективной реализации. В статье определяются понятие и значение экономических методов управления экологическим правопорядком Целью данной работы является постановка вопросов обеспечения экономических методов управления экологическим правопорядком как экономических инструментов в Республике Казахстан, предложение путей решения проблем обеспечения экономических методов управления экологическим правопорядком. Научная и практическая значимость исследования заключается в полученных результатах и выводах. В итоге, в статье сделан вывод о наличии большого комплекса проблем обеспечения экономических методов управления экологическим правопорядком несмотря на огромный объем работы, проведенной в этой сфере. Предлагается повысить эффективность экономических инструментов за счет введения новых инструментов и совершенствования существующих. Повышенный научный интерес в рассматриваемой теме связан с продолжающимися реформами в данной области. Научная статья подготовлена в рамках реализации научно-исследовательского проекта грантового финансирования Комитетом науки Министерства науки и высшего образования Республики Казахстан ИРН AP14872548 на тему: «Современный экологический правопорядок: казахстанский кейс». Ключевые слова: экологический правопорядок, экология, экономические методы управления, экономические инструменты, нормативно-правовая база, природопользование, экологические требования.

Воспаление – универсальный, генетически за программированный комплекс ре акций на по вреждениераз личной при роды на правленный на ликвидацию или ограничение очага по вреждения и вы звавших его патогенных агентов. Воспалительная ре акция – один из ключевых механизмов врожденного иммунитета, биологический смысл кот орогоне только за щита орган изма от патогенов, но и по ддержание, и восстановление по врежденных тканей. Глубокое по нимание механизмов воспаления крайне важно, так как более 70% из вестных за болеваний чело века связано с воспалительным процесс ом, в том числе аутоиммунные, не йродегенеративные, сердечно-со судистые за болевания, диабет и рак. Современная на ука и медицина рас полагает боль шим арсеналом мет одов и инструментов по зволяющих про вести раз ностороннюю оценку со стояния орган изма при воспалительныхпроцесс ах. С каждым год ом ис пользование без инвазивных мет одов ис следований раз ныхпроцесс ов про текающих в орган изме в ре альном времен и ста новится более до ступным и при емлимым для по нимания клеточных процесс ов в дина мике. Одним из со временных инструментов при менимых для на блюдения дина мики иммунных клеток явл яется витальная микроскопия. Данный об зор литературы по священ роли мет ода мультифотонной микроскопии для ис следования механизмов за действованных в форм ировании иммунного от вета. Много фотонная микроскопия дает возможно стьиз учать живые ткани, так какоб ладает вы сокой глубиной про никновения и минимальной фототоксичностью. Использование дан ного мет ода по зволяет на блюдать течение клеточных процесс ов, так их как клеточная миграция, взаимодействие клеток, а так же про водить ис следования биологических сис тем и клеток в не йробиологии, кардиологии и друг их сфер ах.