Although many of the current hypotheses to explain the origin and distribution of the Amazon biodiversity has been based directly or indirectly on geological data, the reconstruction of the geological history of the Amazon region is still inadequate to analyze its relationship with the biodiversity. This work has the main goal to characterize the sedimentary successions formed in the Brazilian Amazon in the Neogene-Quaternary discussing the evolution of the depositional systems through time and analyzing their main controlling mechanisms in order to fill up this gap. Radar image interpretation, sedimentological studies, and radiocarbon dating allowed the mapping of Plio-Pleistocene to Holocene units along the Solimões-Amazonas River, Brazil. This integrated work led to the characterization of five sedimentary successions overlying Miocene deposits of the Solimões/Pebas Formation, which include the following: Içá Formation (Plio-Pleistocene), deposits Q1 (37,400–43,700¹⁴C yr B.P.), deposits Q2 (27,200¹⁴C yr B.P.), deposits Q3 (6730–2480¹⁴C yr B.P.), and deposits Q4 (280–130¹⁴C yr B.P.). These deposits occur mostly to the west of Manaus, forming NW–SE elongated belts that are progressively younger from SW to NE, indicating a subsiding basin with a depocenter that migrated to the NE. The reconstruction of the depositional history is consistent with significant changes in the landscapes. Hence, the closure of a large lake system at the end of the Miocene gave rise to the development of a Plio-Pleistocene fluvial system. This was yet very distinct from the modern drainage, with shallow, energetic, highly migrating, braided to anastomosed channels having an overall northeast outlet. This fluvial system formed probably under climatic conditions relatively drier than today's. During the early Pleistocene, there was pronounced erosion, followed by a renewed depositional phase ca. 40,000¹⁴C yr B.P., with the development of prograding lobes and/or crevasse splays associated with a lake system (i.e., fan-delta) and/or fluvial flood plain areas. After a period of erosion, a fluvial system with eastward draining channels started to develop at around 27,000¹⁴C yr B.P. The fluvial channels were overflooded in mid-Holocene time. This flooding is attributed to an increased period of humidity, with a peak between 5000 and 2500¹⁴C yr B.P. The data presented herein support that, rather than being a monotonous area, the Amazonia was a place with frequent changes in landscape throughout the Neogene-Quaternary, probably as a result of climatic and tectonic factors. We hypothesize that these changes in the physical environment stressed the biota, resulting in speciation and thus had a great impact on modern biodiversity.

Climate change has been identified as the primary threat to the integrity and functioning of ecosystems in this century, although there is still much uncertainty about its effects and the degree of vulnerability for different ecosystems to this threat. Here we propose a new methodological approach capable of measuring and mapping the resilience of terrestrial ecosystems at large scales based on their climatic niche. To do this, we used high spatial resolution remote sensing data and ecological niche modeling techniques to calculate and spatialize the resilience of three stable states of ecosystems in South America: forest, savanna, and grassland. Also, we evaluated the sensitivity of ecosystems to climate stress, the likelihood of exposure to non-analogous climatic conditions, and their respective adaptive capacities in the face of climate change. Our results indicate that forests, the most productive and biodiverse terrestrial ecosystems on the earth, are more vulnerable to climate change than savannas or grasslands. Forests showed less resistance to climate stress and a higher chance of exposure to non-analogous climatic conditions. If this scenario occurs, the forest ecosystems would have less chance of adaptation compared to savannas or grasslands because of their narrow climate niche. Therefore, we can conclude that a possible consolidation of non-analogous climatic conditions would lead to a loss of resilience in the forest ecosystem, significantly increasing the chance of a critical transition event to another stable state with a lower density of vegetation cover (e.g., savanna or grassland).

The lower Miocene Pirabas Formation in the North of Brazil was deposited under influence of the proto-Amazon River and is characterized by large changes in the ecological niches from the early Miocene onwards. To evaluate these ecological changes, the elasmobranch fauna of the fully marine, carbonate-rich beds was investigated. A diverse fauna with 24 taxa of sharks and rays was identified with the dominant groups being carcharhiniforms and myliobatiforms. This faunal composition is similar to other early Miocene assemblages from the proto-Carribbean bioprovince. However, the Pirabas Formation has unique features compared to the other localities; being the only Neogene fossil fish assemblage described from the Atlantic coast of Tropical Americas. Phosphate oxygen isotope composition of elasmobranch teeth served as proxies for paleotemperatures and paleoecology. The data are compatible with a predominantly tropical marine setting with recognized inshore and offshore habitats with some probable depth preferences (e.g., Aetomylaeus groups). Paleohabitat of taxa particularly found in the Neogene of the Americas (†Carcharhinus ackermannii, †Aetomylaeus cubensis) are estimated to have been principally coastal and shallow waters. Larger variation among the few analyzed modern selachians reflects a larger range for the isotopic composition of recent seawater compared to the early Miocene. This probably links to an increased influence of the Amazon River in the coastal regions during the Holocene.

A paleomegafauna site from central Amazonia with exceptional preservation of mastodons and ground sloths allows for the first time a precise age control based on¹⁴C analysis, which, together with sedimentological and δ¹³C isotope data, provided the basis to discuss habitat evolution within the context of climate change during the past 15,000 yr. The fossil-bearing deposits, trapped within a depression in the Paleozoic basement, record three episodes of sedimentation formed on floodplains, with an intermediate unit recording a catastrophic deposition through debris flows, probably favored during fast floodings. The integrated approach presented herein supports a change in humidity in central Amazonia through the past 15,000 yr, with a shift from drier to arboreal savanna at 11,340 (±50)¹⁴C yr B.P. and then to a dense forest like we see today at 4620 (±60)¹⁴C yr B.P.