An approximate revolution synthesis of magnesium oxide (MgO) nanoparticles has been prepared. For plant growth and development, MgO is essential. The effect and efficiency, respectively, in seed germination, seedling growth, and plant growth were also studied. These analyses examined maize with different concentrations and parameters. The concentration of 500 ppm was tested with extreme results in areas such as plant height, protein contents both in-vivo and in-vitro, and MgO effects shown both in shoot (12.83 ± 0.5 cm) and root (5.37 ± 0.5 cm). Maximum confirmations were fixed with the help of MgO NPs characterization through TEM, SEM, FTIR, zeta potential, and X-ray. The effect of MgO NPs showed a significant increase in root and shoot length, and simultaneous in-vivo studies also showed significant results in plant physiological parameters. In effect, the vital performance in germination rate, potential, and index MgO NPs was higher than the control. Moreover, the performance and absorption of MgO NPs was confirmed by physiological characterization with the help of a UV–Vis spectrophotometer applied to the leaves and roots. It was demonstrated that the influence of MgO NPs is positive and potentially can be used for seedling growth and also for plants. It may bolster farming methods, and help maintain high food quality, quantity, and production.

Difenoconazole-loaded (CS-DIF) microcapsules were synthesized by encapsulating difenoconazole into biocompatible chitosan. The physical and chemical properties indicated that the encapsulation and chemical loading rates were 85.58% and 61.98%, respectively. The microcapsules exhibited prominent controlled-release and surface stability performance. The cumulative release rate was only 33.6% in 168 h, and the contact angle decreased by 11.73° at 120 s compared with difenoconazole. The antifungal activity of the CS-DIF microcapsules against Curvularia lunata was confirmed through observations of colony growth, in vitro and in vivo inoculation, mycelium morphology, as well as DNA and protein leakage. The antioxidant enzyme activity of superoxide dismutase, peroxidase, and catalase decreased by 65.1%, 84.9%, and 69.7%, respectively, when Curvularia lunata was treated with 200 μg/mL microcapsules, compared with the control in 24 h. The enzymatic activity of polyphenol oxidase decreased by 323.8%. The reactive oxygen species contents of hydrogen peroxide and superoxide anions increased by 204.6% and 164%, respectively. Additionally, the soluble sugar and soluble protein contents decreased by 65.5% and 69.6%, respectively. These findings provided a novel approach to control the growth of C. lunata efficiently, laying a foundation for reducing the quantity and enhancing the efficiency of chemical pesticides. The CS-DIF microcapsules exhibited a strong inhibitory effect on fungus, effectively preventing and controlling leaf spot disease and showing potential for field applications. This study might be of great significance in ensuring plant protection strategies.