An approximate revolution synthesis of magnesium oxide (MgO) nanoparticles has been prepared. For plant growth and development, MgO is essential. The effect and efficiency, respectively, in seed germination, seedling growth, and plant growth were also studied. These analyses examined maize with different concentrations and parameters. The concentration of 500 ppm was tested with extreme results in areas such as plant height, protein contents both in-vivo and in-vitro, and MgO effects shown both in shoot (12.83 ± 0.5 cm) and root (5.37 ± 0.5 cm). Maximum confirmations were fixed with the help of MgO NPs characterization through TEM, SEM, FTIR, zeta potential, and X-ray. The effect of MgO NPs showed a significant increase in root and shoot length, and simultaneous in-vivo studies also showed significant results in plant physiological parameters. In effect, the vital performance in germination rate, potential, and index MgO NPs was higher than the control. Moreover, the performance and absorption of MgO NPs was confirmed by physiological characterization with the help of a UV–Vis spectrophotometer applied to the leaves and roots. It was demonstrated that the influence of MgO NPs is positive and potentially can be used for seedling growth and also for plants. It may bolster farming methods, and help maintain high food quality, quantity, and production.

Maize is a critical crop for food, feed, and bioenergy worldwide. This study characterized the genetic diversity and population structure of 212 important inbred lines collected from the Southeast China breeding program using the Maize6H-60K single nucleotide polymorphism (SNP) array. To investigate the genetic architecture of plant height (PH) and ear height (EH), genome-wide association analysis (GWAS) was performed on this population in 2021 and 2022. Cluster analysis and population genetic structure analysis grouped the 212 maize inbred lines into 10 distinct categories. GWAS identified significant associations for PH, EH, and the EH/PH ratio. A total of 40 significant SNP (p < 8.55359 × 10⁻⁷) were detected, including nine associated with PH, with phenotypic variation explained (PVE) ranging from 3.42% to 25.92%. Additionally, 16 SNP were linked to EH, with PVE ranging from 2.49% to 38.49%, and 15 SNP were associated with the EH/PH ratio, showing PVE between 3.43% and 16.83%. Five stable SNP, identified across two or more environments, were further analyzed. Three of these SNP loci are reported for the first time in this study: two loci associated with the PH, AX-108020973, and AX-108022922, as well as one new locus, AX-108096437, which was significantly associated with the EH/PH ratio. Additionally, two other significant SNP (AX-247241325 and AX-108097244) were located within a 2 Mb range of previously identified QTL and/or related SNP. Within the 200 kb confidence intervals of these five stable SNP loci, 76 functionally annotated genes were identified. Further functional analysis indicated that 14 of these genes may play a role in regulating plant morphology, which is primarily involved in hormone synthesis, microtubule development, root growth, and cell division regulation. For instance, the homologous genes GRMZM2G375249 and GRMZM2G076029 in maize correspond to OsPEX1 in rice, a protein similar to extension proteins that are implicated in lignin biosynthesis, plant growth promotion, and the negative regulation of root growth through gibberellin-mediated pathways. The candidate gene corresponding to AX-108097244 is GRMZM2G464754; previous studies have reported its involvement in regulating EH in maize. These findings enhance the understanding of QTL associated with maize plant-type traits and provide a foundation for cloning PH, EH-related genes. Therefore, the results also support the development of functional markers for target genes and the breeding of improved maize varieties.

Apple tree canker induced by Valsa mali is a vital disease in apple production around the world, and it highlyimpacts the development of apple industry. It is of great significance to study the inhibition effect of common fungicides and develop new fungistats for comprehensive control of apple tree canker. In this experiment, the inhibition activity of five fungicides, including mancozeb, metalaxyl, iprodione, prochloraz, and difenoconazole along with biosynthesized nanosilver against V. mali, were measured with the mycelium growth rate and agar well diffusion methods. The results showed that iprodione exhibited the best inhibitory effect, the median inhibition concentration (IC₅₀) of iprodione and nanosilver was 0.62 μg.mL⁻¹ and 45.50 μg.mL⁻¹, the suppression rate achieved 67.93% at 200 μg.mL⁻¹ of nanosilver. Moreover, a remarkable additive and synergistic antimicrobial effect was verified when silver nanoparticles were conjugated with iprodione at 9:1, 8:2, 7:3, and 6:4 (v/v), and the toxicity ratio was 1.04, 1.13, 1.01, and 0.98, respectively. It is proven that biosynthesized silver nanoparticles could effectively inhibit Valsa mali, and it is possible to develop and screen silver nanoparticle-based nano pesticides to manage plant diseases synthetically.

Silver nanoparticles were biosynthesized from Conyzacanadensis leaf extract with the help of a microwave oven. The UV-vis spectrum showed the maximum absorption at 441 nm, corresponding to the surface plasmon resonance of silver nanoparticles. Transmission electron microscope and scanning electron microscope images showed that the synthesized silver nanoparticles were spherical or near-spherical with an average diameter of 43.9 nm. X-ray diffraction demonstrated nanoparticles with a single-phase cubic structure. As-synthesized silver nanoparticles displayed prominent antifungal activity against Bipolaris maydis. The colony inhibition rate reached 88.6% when the concentration of nanosilver colloid was 100 μL·mL⁻¹ (v/v). At such a concentration, no colony formation was observed on the solid plate. The diameter of the inhibition zone was 13.20 ± 1.12 mm. These results lay the foundation for the comprehensive control of plant pathogens using an environmentally friendly approach.

Difenoconazole-loaded (CS-DIF) microcapsules were synthesized by encapsulating difenoconazole into biocompatible chitosan. The physical and chemical properties indicated that the encapsulation and chemical loading rates were 85.58% and 61.98%, respectively. The microcapsules exhibited prominent controlled-release and surface stability performance. The cumulative release rate was only 33.6% in 168 h, and the contact angle decreased by 11.73° at 120 s compared with difenoconazole. The antifungal activity of the CS-DIF microcapsules against Curvularia lunata was confirmed through observations of colony growth, in vitro and in vivo inoculation, mycelium morphology, as well as DNA and protein leakage. The antioxidant enzyme activity of superoxide dismutase, peroxidase, and catalase decreased by 65.1%, 84.9%, and 69.7%, respectively, when Curvularia lunata was treated with 200 μg/mL microcapsules, compared with the control in 24 h. The enzymatic activity of polyphenol oxidase decreased by 323.8%. The reactive oxygen species contents of hydrogen peroxide and superoxide anions increased by 204.6% and 164%, respectively. Additionally, the soluble sugar and soluble protein contents decreased by 65.5% and 69.6%, respectively. These findings provided a novel approach to control the growth of C. lunata efficiently, laying a foundation for reducing the quantity and enhancing the efficiency of chemical pesticides. The CS-DIF microcapsules exhibited a strong inhibitory effect on fungus, effectively preventing and controlling leaf spot disease and showing potential for field applications. This study might be of great significance in ensuring plant protection strategies.