Türkiye buğday faunası için yeni bir tür, Meloidogyne artiellia (Franklin)'nın morfolojik ve moleküler yöntemlerle tanımlanması

Tr en Bu çalışma ile 2018-2019 yıllarında Niğde'deki başlıca tahıl yetiştirme alanlarında bulunan fitofag nematodların yoğunluk ve dağılımı belirlenmiş, tahıl kist nematodu türlerinin morfolojik ve moleküler yöntemlerle tanımı yapılmıştır. Fitofag nematodlar 12 cins olarak toprak örneklerinin %95'inde tespit edilmiştir. En yaygın fitofag nematodlar sırasıyla, Heterodera, Ditylenchus, Merlinius, Pratylenchus, Aphelenchus, Aphelenchoides, Tylenchus, Helicotylenchus, Trophurus, Pratylenchoides, Filenchus ve Xiphinema cinsleridir (bulunma yoğunluğuna göre sıralanmıştır). İncelenen toprak örneklerinin %75’inde tahıl kist nematodu (Heterodera spp.) bulunmuştur. Kistlerin ve 2. dönem larvaların ölçümleri ve morfolojik karakterlerinin değerlendirilmesiyle elde edilen sonuçlarda Heterodera filipjevi (Madzhidov, 1981) Stelter, 1984 olarak tespit edilmiş olup sadece Çamardı’dan iki popülasyon Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) olarak belirlenmiştir. Heterodera filipjevi’nin tür içi varyasyonlarına bakıldığında hepsinin aynı gurupta olabileceği değerlendirilmiştir. Buna ilave olarak tahıl kist nematodları yanında kök lezyon nematodlarının, Pratylenchus türlerinin de rotasyon yapılan/yapılmayan Niğde İli tarım alanlarında yüksek yoğunlukta bulunduğu sonucuna varılmıştır. This study evaluated the occurrence and incidence of phytophagous nematodes and identified the cereal cyst nematode species by morphological and molecular tools in the main cereal-growing areas in Niğde in 2018-2019. Phytophagous nematodes within twelve genera were detected in 95% of soil samples. The most common phytophagous nematodes in cereal soil were in the genera Heterodera, Ditylenchus, Merlinius, Pratylenchus, Aphelenchus, Aphelenchoides, Tylenchus, Helicotylenchus, Trophurus, Pratylenchoides, Filenchus and Xiphinema (in decreasing order of incidence). In particular, 75% of the soil samples from surveyed fields were infested with the cereal cyst nematodes (Heterodera spp.). Morphological characteristics of cysts and second-stage juveniles were calculated within the expected ranges for Heterodera filipjevi (Madzhidov, 1981) Stelter, 1984, however, two populations from Çamardı was determined as Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae). Intraspecific variation was not observed within the populations of H. filipjevi which could be in the same genotypic group. In addition to the high incidence of these Heterodera spp., intensive cereal cropping systems with/without non-cereal rotations in wheat production areas of Niğde also resulted in high incidence of root lesion nematode, Pratylenchus species.

Heterodera schachtii (Schmidt, 1871) (Nematoda: Heteroderidae) is one of the most widespread plant-parasitic nematodes (PPNs) associated with cabbages, which cause severe yield losses in cruciferous vegetables. This study aimed to improve the current understanding of the prevalence and detection of H. schachtii in the cabbage-growing areas of Niğde Province, Türkiye. Field surveys were conducted between November and December 2021, and 100 soil samples were collected immediately after cabbage harvesting. Heterodera schachtii populations were identified by morphological and morphometric methods along with Internal Transcribed Spacer of the ribosomal region (ITS-rDNA) and Cytochrome Oxidase Subunit (COI-mtDNA) sequencing. The mean body length of H. schachtii was 463 ± 7 μm, while stylet and hyaline length ranged between 20.7–27.8 μm and 20.1–32.1 μm, respectively. Nearly half of the surveyed areas were infested with H. schachtii with a 41% incidence rate. However, the Merkez District had the highest proportion of infested fields with an over 51% incidence rate. The population density was determined in 41 samples with a mean of 79.5 cysts per 250 g of soil. These results will help to determine the control and management strategies of H. schachtii.