The study was conducted to determine the occurrence, frequency and density of species of Meloidogyne Goeldi, 1892 (Tylenchida: Meloidogynidae) in ware potato cultivation areas of Turkey in Plant Protection Central Research Institute. Soil samples were taken from Afyonkarahisar (149), Aksaray (69), Bolu (94), Kayseri (94), Konya (127), Nevşehir (91), Niğde (226) and Sivas (77) Provinces in 2018-2019. Meloidogyne juveniles were extracted by modified Baarmenn funnel and counted under inverted microscope. Meloidogyne populations were identified by species-specific primers. Meloidogyne spp. were detected in 84 of 927 soil samples. Meloidogyne spp. was detected in Aksaray, Kayseri, Nevşehir, and Niğde while was not found Afyonkarahisar, Bolu, Konya, and Sivas Provinces. In survey areas, the occurrence of Meloidogyne chitwoodi Golden, O'Bannon, Santo & Finley, 1980 and Meloidogyne hapla (Chitwood, 1949) (Tylenchida: Meloidogynidae) was 8.7 and 1.5%, respectively. Also, M. chitwoodi and M. hapla mixed populations were found in 1.2% of samples. Mean density of Meloidogyne spp. J2s was determined as 182, 175, 162 and 90 J2s/100 ml of soil in Niğde, Nevşehir, Aksaray and Kayseri Provinces, respectively. In conclusion, important ware potato cultivation areas of Turkey were found to be infested with Meloidogyne spp., but the important seed potato-growing areas were found to be free of Meloidogyn e spp.

Tr en Bu çalışmada iki yerel entomopatojen nematod izolatının (Steinernema feltiae-KV06 (Filipjev) (Rhabditida: Steinernematidae) ve Heterorhabditis bacteriophora-EO7 (Rhabditida: Heterorhabtidae) Blatta lateralis Walker (Blattodea: Blattidae)’in biyolojik mücadelesindeki kullanım olanakları araştırılmıştır. Çalışmada kullanılan izolatlar Ankara ili Kelebekler Vadisi ve Eğriova Tabiat Parklarından elde edilmiştir. İzolatlar, 9cm çapındaki petri kutularına bırakılan B. lateralis nimflerine 100, 250, 500 ve 1000 IJ/cm² dozlarında uygulanmıştır. B. lateralis nimflerindeki ölümler, uygulamadan 2, 4, 6, 8, 10, 12 ve 14gün sonra kaydedilmiştir. 1000 IJ/cm²'de, S. feltiae-KV06 ve H. bacteriophora-EO7’nın, LT₅₀ değerleri 2.3 ve 2.6 gün ve LT₉₀ değerleri ile sırasıyla 6.6 ve 7.9 gün ile en yüksek ölüm oranına (% 100.0 ± 0.0) neden olmuştur. Her iki izolattaki en düşük ölüm yüzdesi, 2 gün maruziyetten sonra 100IJs/cm²'de belirlenmiştir (S. feltiae-KV06: 0.6±1.4 ve H. bacteriophora-EO7: 0.0±0.0). Farklı zaman aralıklarındaki LC_50,90 değerleri her iki izolat arasında gözardı edilebilecek düzeyde farklılık göstermekle birlikte, S. feltiae-KV06’nın B. lateralis nimflerine karşı H. bacteriophora-EO7’dan daha etkili olduğu görülmektedir. Bununla birlikte, her iki izolatın da farklı sıcaklık, nem ve beton, parke ve fayans gibi farklı yüzeylerde de zararlı üzerindeki etkinliklerinin test edilmesi gerekmektedir. Two Turkish entomopathogenic nematodes isolates, Steinernema feltiae-KV06 (Filipjev) (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora-EO7 (Rhabditida: Heterorhabtidae), were tested against the cockroach, Blatta lateralis Walker (Blattodea: Blattidae) to determine their potential for its control. The isolates were obtained from Kelebekler Vadisi and Eğriova Nature Parks in Ankara Province, Turkey. These isolates were applied at concentrations of 100, 250, 500 and 1000 IJ/cm² to B. lateralis nymphs in 9cm diameter Petri dishes. The mortalities were recorded at 2, 4, 6, 8, 10, 12 and 14 days after application. At 1000 IJs/cm², S. feltiae-KV06 and H. bacteriophora-EO7 caused the highest mortality ( 100.0±0.0 %) with an LT₅₀ of 2.3 and 2.6 days, respectively, and an LT₉₀ of 6.6 and 7.9 days, respectively. The lowest mortality rate caused by both species was at 100IJs/cm² after 2 days of exposure (S. feltiae-KV06: 0.6±1.4 ; H. bacteriophora-EO7: 0.0±0.0 ). There was no significant difference in the efficacy of the isolates at all concentrations and for all time periods. The effectiveness of both isolates against this pest should be tested at different temperatures and humidities and on different surfaces such as ceramic, concrete and parquet.

Ditylenchus dipsaci (Kühn, 1857) Filipjev, 1936 (Rhabditida: Anguinidae) is one of the destructive agents of garlic and reduces yield and market value. One of the most practical and eco-friendly methods for nematode management is using resistant varieties. In the study, two endemic garlic species, Allium tuncelianum (Kolman) Ozhatay, Mathew & Siraneci and Allium macrochaetum subsp. macrochaetum Boiss. & Hausskn. (Alliaceae: Amaryllidaceae), 10 mutant and 32 landraces garlic genotypes, Allium sativum L. (Alliaceae: Amaryllidaceae) were investigated for their resistance reactions to D. dipsaci and effect of D. dipsaci on some plant growth parameters. All experiments were conducted at Atatürk Horticultural Central Research Institute in 2019-2020. None of the genotypes was found resistant to D. dipsaci, and reproduction factors, which ranged from 2.6 to 12.7, were grouped from susceptible to highly susceptible. The Tunceli garlic genotype had the lowest reproduction factor (2.6), 36.6% less than the highly susceptible Muğla6 genotype. Alata1, Muğla1, Muğla7 and Kula genotypes had the lowest decrease rate with nematode treatment at least in one of the plant growth parameters. The genotypes that had lower nematode multiplication and displayed better development under nematode infestation in this study are recommended for the field infested with D. dipsaci as sources for garlic breeding.

Investigating alternatives to synthetic chemicals in pest management is essential. The bioactivity of Artemisia absinthium and A. dracunculus essential oils (EOs) was evaluated against two stored-product insect pests (Sitophilus granaries and Tribolium castaneum) and two root-knot nematodes (Meloidogyne incognita and M. chitwoodi). EOs were hydrodistilled and analyzed by GC-MS: A. absinthium consisted mainly of myrtenyl acetate (47.10%) and β-thujone (11.41%); A. dracunculus of methyleugenol (26.02%), β-asarone (14.04%), and elemicin (10.95%). In contact bioassays, A. absinthium caused 93.2–96.8% mortality against S. granarius, but minimal effect on T. castaneum. A. dracunculus caused 66.3–73.5% mortality against S. granarius, no effect on T. castaneum, yet reduced progeny by 87%. Both EOs strongly repelled T. castaneum but weakly affected S. granarius. A. dracunculus exhibited 100% nematicidal efficacy against M. incognita and 94.9% against M. chitwoodi; A. absinthium showed limited effect. A. dracunculus showed minimal hatching inhibition (<30%), whereas A. absinthium increased hatching. Sparse Partial Least Squares (sPLS) prioritized pulegone, methyleugenol, β-asarone, β-thujone, myrtenyl acetate, and β-phellandrene as constituents co-varying with AChE/BChE responses, with docking identifying pulegone as the top ligand. These findings demonstrate species-specific bioactivity patterns: A. dracunculus shows strong nematicidal and reproductive inhibitory effects, whereas A. absinthium shows potent acute toxicity and repellency against S. granarius, supporting multi-target biopesticide strategies to mitigate risks of synthetic chemical treatments.