Transepidermal drug delivery achieves high drug concentrations at the action site and ensures continuous drug delivery and better patient compliance with fewer adverse effects. However, drug delivery through topical application is still limited in terms of drug penetration. Chitosan is a promising enhancer to overcome this constraint, as it can enhance drug diffusion by opening the tight junctions of the stratum corneum. Therefore, here, we developed a novel chitosan nanosponge (CNS) with an optimal ratio and molecular weight of chitosan to improve drug penetration through skin. To prepare the CNS, two types of chitosan (3 and 10 kDa) were each conjugated with poloxamer 407 using para-nitrophenyl chloroformate, and the products were mixed with poloxamer 407 at ratios of 5:5, 8:2, and 10:0. The resulting mixtures were molded to produce flexible soft nanosponges by simple nanoprecipitation. The CNSs were highly stable in biological buffer for four weeks and showed no toxicity in human dermal fibroblasts. The CNSs increased drug permeability through human cadaver skin in a Franz-type diffusion cell, with substantially higher permeability with 3 kDa chitosan at a ratio of 8:2. This suggests the applicability of the novel CNS as a promising carrier for efficient transepidermal drug delivery.

Antimicrobial air filters are required to protect humans from the risk of secondary bioaerosol pollution as well as airborne particles. Three plant extracts (tea-tree oil, rosemary, and garlic) were selected to replace antimicrobial chemicals in air filters. The antimicrobial activity of plant extracts was investigated using Micrococcus luteus and Escherichia coli. Phytochemicals present in the three plant extracts were identified using a gas chromatograph coupled with a mass spectrometer. The extracts were spray-coated on polyethylene terephthalate filter surfaces using silicate polymeric coating and evaluated via X-ray photoelectron spectroscopy and a scanning electron microscope with energy dispersive spectroscopy. After coating, an increase of 9.1% in the pressure drop was observed. The strain Micrococcus luteus was used to evaluate the antimicrobial activity of the air filter. After bioaerosol exposure, the tea-tree oil-coated filters immediately induced M. luteus cell inactivation (40–55%), whereas the rosemary and garlic coated filters did not. However, 48 h after exposure, a significant M. luteus inactivation of 99.99%, 99.0%, and 99.9% was recorded for concentrations of 2.89, 6.73, and 11.51 mg/cm² for the tea-tree, rosemary, and garlic extracts, respectively. The coated filters exhibited high antimicrobial activity, thereby indicating significant potential for application as self-cleaning air filters.

A key aspect of biochip and biosensor preparation is optimization of the optical or electrochemical techniques that combine high sensitivity and specificity. Among them, optical techniques such as the use of fluorescent polymeric nanoparticles have resulted in dramatic progress in the field of diagnostics due to their range of advantages. We herein report a facile approach for the development of novel fluorescein polymeric nanoparticles (FPNPs) with immobilization of specific biomolecules for application in a highly sensitive optical biosensor. A series of three amphiphilic fluorescein polymers (poly(FMA-r-NAS-r-MA)), comprising hydrophobic fluorescein O-methacrylate (FMA), hydrophilic N-acryloxysuccinimide (NAS), and methacrylic acid (MA) monomers were synthesized through radical polymerization. In an aqueous environment, these fluorescein polymers self-assembled into spherical shaped nanoparticles with a well-defined particle size, narrow particle size distribution, and enhanced fluorescence properties. The bio-immobilization properties of the FPNPs were also tunable by control of the activated N-hydroxysuccinimide ester group in the polymer series. Furthermore, the fluorescence sensitivity of bovine serum albumin detection by the FPNPs indicates that the limit of detection and sensitivity were improved compared to conventional fluorescence dye-labelled proteins. These novel FPNPs therefore represent a suitable technology for disease diagnosis and biomarker detection to ultimately improve the sensitivity of existing analytical methodologies in a facile and cost-effective manner.

The global burden of bone-related diseases is increasing in the aging society; thus, improved bone targeted imaging for their early identification and treatment are needed. In this study, we screened novel peptide ligands for hydroxyapatite, a major inorganic component of teeth and bones, and identified a peptide enabling in vivo bone targeting and real-time fluorescence bone detection. To isolate peptides highly specific for hydroxyapatite, we used negative and positive selection from a randomized 8-mer peptide phage library and identified hydroxyapatite-specific peptides (HA-pep2, HA-pep3, and HA-pep7). Among these three peptides, HA-pep3 showed the highest binding capacity and superior dissociation constant towards hydroxyapatite surfaces over time (~ 88.3% retained on hydroxyapatite after two weeks). Furthermore, HA-pep3 was highly specific for hydroxyapatite compared to other calcium salt-based materials. Using this superior specificity, HA-pep3 showed higher accumulation in skull, spine, and joints in comparison with scrambled control peptide during real-time whole-body imaging. Ex vivo analysis of the major organs and bone from mice demonstrated that the fluorescence intensity in bone was about 3.32 folds higher in the case of HA-pep3 than the one exhibited by the scrambled control peptide. Our study identified a novel approach for targeting ligands for bone specific imaging and can be useful for drug delivery applications.

We determined the potential of three mechanical pulverizers—a continuous ball mill (CBM), an air classifier mill (ACM), and a high-speed mill (HSM)—in the continuous pretreatment of corn stover. The mean diameters of the pulverized biomasses were not significantly different in the three cases, and the glucose yields from the CBM-, ACM-, and HSM-pulverized samples were 29%, 49%, and 44%, respectively. The energy requirements and process capacities for the ACM and HSM were similar. We conclude that the ACM and HSM could be used in the continuous pretreatment of corn stover and would be useful in biofuel production.