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Dr. Jin Hyung Lee
Korea Institute of Ceramic Engineering and Technology (KICET)

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0 Silica
0 bioceramics
0 Biomass and bioenergy
0 pretreatment technologies
0 Biogenic synthesis

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Journal article
Published: 27 July 2021 in Pharmaceutics
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Cancer, which is a leading cause of death, contributes significantly to reducing life expectancy worldwide. Even though paclitaxel (PTX) is known as one of the main anticancer drugs, it has several limitations, including low solubility in aqueous solutions, a limited dosage range, an insufficient release amount, and patient resistance. To overcome these limitations, we suggest the development of PTX-loaded thermosponge nanoparticles ([email protected]), which result in improved anticancer effects, via a simple nanoprecipitation method, which allows the preparation of [email protected] with hydrophobic interactions without any chemical conjugation. Further, to improve the drug content and yield of the prepared complex, the co-organic solvent ratio was optimized. Thus, it was observed that the drug release rate increased as the drug capacity of [email protected] increased. Furthermore, increasing PTX loading led to considerable anticancer activity against multidrug resistance (MDR)-related colorectal cancer cells (HCT 15), implying a synergistic anticancer effect. These results suggest that the solubilization of high drug amounts and the controlled release of poorly water-soluble PTX using TNPs could significantly improve its anticancer therapy, particularly in the treatment of MDR-p-glycoprotein-overexpressing cancers.

ACS Style

Jin Sil Lee; Hyeryeon Oh; Daekyung Sung; Jin Hyung Lee; Won Il Choi. High Solubilization and Controlled Release of Paclitaxel Using Thermosponge Nanoparticles for Effective Cancer Therapy. Pharmaceutics 2021, 13, 1150 .

AMA Style

Jin Sil Lee, Hyeryeon Oh, Daekyung Sung, Jin Hyung Lee, Won Il Choi. High Solubilization and Controlled Release of Paclitaxel Using Thermosponge Nanoparticles for Effective Cancer Therapy. Pharmaceutics. 2021; 13 (8):1150.

Chicago/Turabian Style

Jin Sil Lee; Hyeryeon Oh; Daekyung Sung; Jin Hyung Lee; Won Il Choi. 2021. "High Solubilization and Controlled Release of Paclitaxel Using Thermosponge Nanoparticles for Effective Cancer Therapy." Pharmaceutics 13, no. 8: 1150.

Journal article
Published: 30 June 2021 in Sustainability
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A two-stage continuous process was developed for improved silica extraction from rice husk. The two-stage continuous process consists of attrition ball milling and alkaline leaching methods. To find the optimum conditions for the continuous process, the effects of alkaline leaching parameters, such as the alkaline solution type and reaction conditions, on the silica extraction yield were investigated in a batch process. The use of NaOH showed a slightly higher silica yield than KOH. The optimum reaction conditions were found to be 0.2 M, 80 °C, 3 h, and 6% (w/v) for the reaction concentration, temperature, duration time, and solid content, respectively. Attrition ball milling was used to make micron-sized rice husk particles and to improve the fluidity of the rice husk slurry. The two-stage continuous process was performed using optimum conditions as determined based on the results of the batch experiment. The two-stage continuous extraction was stably operated for 80 h with an 89% silica yield. During the operation, the solid content remained consistent at 6% (w/v). The obtained silica was characterized using inductively coupled plasma–optical emission spectrometry (ICP–OES), X-ray diffraction (XRD), and the Brunauer–Emmett–Teller (BET) method.

ACS Style

Ji Park; Yang Gu; Seon Park; Ee Hwang; Byoung-In Sang; Jinyoung Chun; Jin Lee. Two-Stage Continuous Process for the Extraction of Silica from Rice Husk Using Attrition Ball Milling and Alkaline Leaching Methods. Sustainability 2021, 13, 7350 .

AMA Style

Ji Park, Yang Gu, Seon Park, Ee Hwang, Byoung-In Sang, Jinyoung Chun, Jin Lee. Two-Stage Continuous Process for the Extraction of Silica from Rice Husk Using Attrition Ball Milling and Alkaline Leaching Methods. Sustainability. 2021; 13 (13):7350.

Chicago/Turabian Style

Ji Park; Yang Gu; Seon Park; Ee Hwang; Byoung-In Sang; Jinyoung Chun; Jin Lee. 2021. "Two-Stage Continuous Process for the Extraction of Silica from Rice Husk Using Attrition Ball Milling and Alkaline Leaching Methods." Sustainability 13, no. 13: 7350.

Journal article
Published: 09 April 2021 in Energies
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The impact of attrition ball-mill pretreatment on food waste particle size, soluble chemical oxygen demand (SCOD), biochemical methane potential, and microbial community during anaerobic digestion was investigated based on milling speed and time. The uniformity of particle size improved with increasing milling speed and time. The SCOD of the pretreated samples increased to 4%, 7%, and 17% at the speeds of 150, 225, and 300 rpm, respectively, compared to the control. Milling time did not significantly change the SCOD. The cumulative methane productions of 430, 440, and 490 mL/g-VS were observed at the speeds of 150, 225, and 300 rpm, respectively, while the untreated sample exhibited the cumulative methane production of 390 mL/g-VS. Extended milling time did not improve methane production much. When the milling times of 10, 20, and 30 min were applied with the milling speed fixed at 300 rpm, the methane productions of 490, 510, and 500 mL/g-VS were observed respectively. Ball-mill pretreatment also increased the total volatile fatty acids. During the anaerobic digestion (AD) of ball-mill treated food waste, acetoclastic methanogens predominated, with a relative abundance of 48–49%. Interestingly, hydrogenotrophic methanogens were 1.6 times higher in the pretreated samples than those in the control. These results showed the potential of attrition ball milling as a food waste pretreatment for improving methane production.

ACS Style

Yang Gu; Seon Park; Ji Park; Byoung-In Sang; Byoung Jeon; Hyunook Kim; Jin Lee. Impact of Attrition Ball-Mill on Characteristics and Biochemical Methane Potential of Food Waste. Energies 2021, 14, 2085 .

AMA Style

Yang Gu, Seon Park, Ji Park, Byoung-In Sang, Byoung Jeon, Hyunook Kim, Jin Lee. Impact of Attrition Ball-Mill on Characteristics and Biochemical Methane Potential of Food Waste. Energies. 2021; 14 (8):2085.

Chicago/Turabian Style

Yang Gu; Seon Park; Ji Park; Byoung-In Sang; Byoung Jeon; Hyunook Kim; Jin Lee. 2021. "Impact of Attrition Ball-Mill on Characteristics and Biochemical Methane Potential of Food Waste." Energies 14, no. 8: 2085.

Journal article
Published: 18 January 2021 in Energies
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This study evaluated Al-ZSM-5 nanocrystals grown from silicalite-1 seed crystals as catalysts for the methane dehydroaromatization (MDA) reaction. Silicalite-1 seed crystals sized between 30 and 40 nm were used to grow Al-ZSM-5 under various synthesis conditions. The size of Al-ZSM-5 was significantly affected by the Si/Al ratio (SAR), synthesis time, and silica nutrients/seed crystal ratio (NSR). Larger crystals were obtained with an increased SAR in the synthesis sols. Gradual growth of Al-ZSM-5 occurred with synthesis time, although the growth in crystal size ceased at 5 h of synthesis at 120 °C, indicating the rapid growth of Al-ZSM-5 aided by the silicalite-1 seeds. Precise tuning of Al-ZSM-5 size was possible by changing the nutrient/silicalite-1 seed ratio; a higher NSR led to larger crystals. Two representative Al-ZSM-5 crystals with SARs of 35 and 140 were prepared for catalyst testing, and the crystal sizes were tailored to <100 nm by controlling NSR. The MDA reaction was conducted in the presence of the prepared Al-ZSM-5. The catalyst size exhibited distinct differences in catalyst stability, while the SAR of catalysts did not produce noticeable changes in the catalyst stability of the Al-ZSM-5 crystals and commercial zeolites in this reaction system.

ACS Style

Hyun Su Kim; Su Kyung Kang; Haoxiang Zhang; Elsa Tsegay Tikue; Jin Hyung Lee; Pyung Soo Lee. Al-ZSM-5 Nanocrystal Catalysts Grown from Silicalite-1 Seeds for Methane Conversion. Energies 2021, 14, 485 .

AMA Style

Hyun Su Kim, Su Kyung Kang, Haoxiang Zhang, Elsa Tsegay Tikue, Jin Hyung Lee, Pyung Soo Lee. Al-ZSM-5 Nanocrystal Catalysts Grown from Silicalite-1 Seeds for Methane Conversion. Energies. 2021; 14 (2):485.

Chicago/Turabian Style

Hyun Su Kim; Su Kyung Kang; Haoxiang Zhang; Elsa Tsegay Tikue; Jin Hyung Lee; Pyung Soo Lee. 2021. "Al-ZSM-5 Nanocrystal Catalysts Grown from Silicalite-1 Seeds for Methane Conversion." Energies 14, no. 2: 485.

Review
Published: 21 December 2020 in Sustainability
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The development of engineered silica particles by using low-cost renewable or waste resources is a key example of sustainability. Rice husks have emerged as a renewable resource for the production of engineered silica particles as well as bioenergy. This review presents a state-of-the-art process for the development of engineered silica particles from rice husks via a bottom-up process. The first part of this review focuses on the extraction of Si from rice husks through combustion and chemical reactions. The second part details the technologies for synthesizing engineered silica particles using silicate obtained from rice husks. These include technologies for the precipitation of silica particles, the control of morphological properties, and the synthesis of ordered porous silica particles. Finally, several issues that need to be resolved before this process can be commercialized are addressed for future research.

ACS Style

Jinyoung Chun; Jin Hyung Lee. Recent Progress on the Development of Engineered Silica Particles Derived from Rice Husk. Sustainability 2020, 12, 10683 .

AMA Style

Jinyoung Chun, Jin Hyung Lee. Recent Progress on the Development of Engineered Silica Particles Derived from Rice Husk. Sustainability. 2020; 12 (24):10683.

Chicago/Turabian Style

Jinyoung Chun; Jin Hyung Lee. 2020. "Recent Progress on the Development of Engineered Silica Particles Derived from Rice Husk." Sustainability 12, no. 24: 10683.

Journal article
Published: 20 December 2020 in Applied Sciences
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Antimicrobial air filters are required to protect humans from the risk of secondary bioaerosol pollution as well as airborne particles. Three plant extracts (tea-tree oil, rosemary, and garlic) were selected to replace antimicrobial chemicals in air filters. The antimicrobial activity of plant extracts was investigated using Micrococcus luteus and Escherichia coli. Phytochemicals present in the three plant extracts were identified using a gas chromatograph coupled with a mass spectrometer. The extracts were spray-coated on polyethylene terephthalate filter surfaces using silicate polymeric coating and evaluated via X-ray photoelectron spectroscopy and a scanning electron microscope with energy dispersive spectroscopy. After coating, an increase of 9.1% in the pressure drop was observed. The strain Micrococcus luteus was used to evaluate the antimicrobial activity of the air filter. After bioaerosol exposure, the tea-tree oil-coated filters immediately induced M. luteus cell inactivation (40–55%), whereas the rosemary and garlic coated filters did not. However, 48 h after exposure, a significant M. luteus inactivation of 99.99%, 99.0%, and 99.9% was recorded for concentrations of 2.89, 6.73, and 11.51 mg/cm2 for the tea-tree, rosemary, and garlic extracts, respectively. The coated filters exhibited high antimicrobial activity, thereby indicating significant potential for application as self-cleaning air filters.

ACS Style

Ha Ram Byun; Seon Young Park; Ee Taek Hwang; Byoung In Sang; Jiho Min; Daekyung Sung; Won Il Choi; Sunghyun Kim; Jin Hyung Lee. Antimicrobial Air Filter Coating with Plant Extracts Against Airborne Microbes. Applied Sciences 2020, 10, 9120 .

AMA Style

Ha Ram Byun, Seon Young Park, Ee Taek Hwang, Byoung In Sang, Jiho Min, Daekyung Sung, Won Il Choi, Sunghyun Kim, Jin Hyung Lee. Antimicrobial Air Filter Coating with Plant Extracts Against Airborne Microbes. Applied Sciences. 2020; 10 (24):9120.

Chicago/Turabian Style

Ha Ram Byun; Seon Young Park; Ee Taek Hwang; Byoung In Sang; Jiho Min; Daekyung Sung; Won Il Choi; Sunghyun Kim; Jin Hyung Lee. 2020. "Antimicrobial Air Filter Coating with Plant Extracts Against Airborne Microbes." Applied Sciences 10, no. 24: 9120.

Journal article
Published: 01 September 2020 in Polymers
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Suppression of pathogenic bacterial growth to increase food and agricultural productivity is important. We previously developed novel hexapeptides (KCM12 and KCM21) with antimicrobial activities against various phytopathogenic bacteria and N2 plasma-treated buffer (NPB) as an alternative method for bacterial inactivation and as an antibiofilm agent of crops. Here, we developed an enhanced antibiofilm method based on antimicrobial hexapeptides with N2 plasma-treated buffer against plant pathogens. Our results demonstrated that hexapeptides effectively inhibited the growth of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and the biofilm it formed. Potent biofilm formation-inhibiting effects of hexapeptides were observed at concentrations of above 20 µM, and samples treated with hexapeptide above 100 µM reduced the ability of the bacteria to produce biofilm by 80%. 3D confocal laser scanning microscopy imaging data revealed that the antimicrobial activity of hexapeptides was enough to affect the cells embedded inside the biofilm. Finally, combination treatment with NPB and antimicrobial hexapeptides increased the antibiofilm effect compared with the effect of single processing against multilayered plant pathogen biofilms. These findings show that the combination of hexapeptides and NPB can be potentially applied for improving crop production.

ACS Style

BoHyun Kim; Hyemi Seo; Jin Hyung Lee; Sunghyun Kim; Won Il Choi; Daekyung Sung; Eunpyo Moon. Enhanced Antibiofilm Effects of N2 Plasma-Treated Buffer Combined with Antimicrobial Hexapeptides Against Plant Pathogens. Polymers 2020, 12, 1992 .

AMA Style

BoHyun Kim, Hyemi Seo, Jin Hyung Lee, Sunghyun Kim, Won Il Choi, Daekyung Sung, Eunpyo Moon. Enhanced Antibiofilm Effects of N2 Plasma-Treated Buffer Combined with Antimicrobial Hexapeptides Against Plant Pathogens. Polymers. 2020; 12 (9):1992.

Chicago/Turabian Style

BoHyun Kim; Hyemi Seo; Jin Hyung Lee; Sunghyun Kim; Won Il Choi; Daekyung Sung; Eunpyo Moon. 2020. "Enhanced Antibiofilm Effects of N2 Plasma-Treated Buffer Combined with Antimicrobial Hexapeptides Against Plant Pathogens." Polymers 12, no. 9: 1992.

Journal article
Published: 07 September 2019 in Journal of Industrial and Engineering Chemistry
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Rice husk is a promising abundant bioresource for the production of high value-added silica materials because it has the highest SiO2 content among all plant-based resources. In this study, ordered mesoporous silica with various pore structures are synthesized from rice husk by combining acid leaching, chemical dissolution, and co-assembly with additional surfactants. Depending upon the type of the surfactant used and the co-assembly conditions, various mesoporous silica that have controlled pore structures (mesocellular forms and hexagonal nanochannel structures), pore sizes (3–60 nm), large surface areas (297–895 m2 g−1), and pore volumes (0.81–1.77 cm3 g−1) are successfully synthesized from a sodium silicate solution, which was made from high-purity silica (99.8%) extracted from rice husk. The synthesis of high value-added silica from an abundant bioresource can open up new avenues for sustainable and environment-friendly industrial development.

ACS Style

Jinyoung Chun; Yang Mo Gu; Jongkook Hwang; Kyeong Keun Oh; Jin Hyung Lee. Synthesis of ordered mesoporous silica with various pore structures using high-purity silica extracted from rice husk. Journal of Industrial and Engineering Chemistry 2019, 81, 135 -143.

AMA Style

Jinyoung Chun, Yang Mo Gu, Jongkook Hwang, Kyeong Keun Oh, Jin Hyung Lee. Synthesis of ordered mesoporous silica with various pore structures using high-purity silica extracted from rice husk. Journal of Industrial and Engineering Chemistry. 2019; 81 ():135-143.

Chicago/Turabian Style

Jinyoung Chun; Yang Mo Gu; Jongkook Hwang; Kyeong Keun Oh; Jin Hyung Lee. 2019. "Synthesis of ordered mesoporous silica with various pore structures using high-purity silica extracted from rice husk." Journal of Industrial and Engineering Chemistry 81, no. : 135-143.

Journal article
Published: 01 June 2019 in Water-Energy Nexus
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Lignocellulosic biomass can be used for producing biochemicals and biofuels through a sugar platform. However, the required pretreatment processes and hydrolysate conditioning typically involve high operational and installation costs. This study investigated the possibility of facile biofuel production using an attrition mill. After milling for one hour, 73.5% (w/w) of corn stover particles had diameters of less than 100 µm, with a mean diameter of 61.3 ± 3.3 µm. Attrition milling of corn stover produced only 0.39 ± 0.01 g L-1 soluble phenolic compounds. The enzymatic conversion of corn stover particles less than 100 µm was 79.8% after 72 h enzymatic hydrolysis. The hydrolysate (i.e., natural sugar) was not inhibitory towards the growth of Saccharomyces cerevisiae. The attrition mill did not require high temperature or pressure conditions and did not inhibit cell growth. Therefore, when the attrition mill is used for pretreatment, a simple biofuel process that does not require washing, detoxification, and solvent recovery could be feasible.

ACS Style

Yang Mo Gu; Ha Ram Byun; Yang-Hoon Kim; Dae-Young Park; Jin Hyung Lee. Assessing the potential of facile biofuel production from corn stover using attrition mill treatment. Water-Energy Nexus 2019, 2, 46 -49.

AMA Style

Yang Mo Gu, Ha Ram Byun, Yang-Hoon Kim, Dae-Young Park, Jin Hyung Lee. Assessing the potential of facile biofuel production from corn stover using attrition mill treatment. Water-Energy Nexus. 2019; 2 (1):46-49.

Chicago/Turabian Style

Yang Mo Gu; Ha Ram Byun; Yang-Hoon Kim; Dae-Young Park; Jin Hyung Lee. 2019. "Assessing the potential of facile biofuel production from corn stover using attrition mill treatment." Water-Energy Nexus 2, no. 1: 46-49.

Journal article
Published: 13 April 2019 in Energies
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We determined the potential of three mechanical pulverizers—a continuous ball mill (CBM), an air classifier mill (ACM), and a high-speed mill (HSM)—in the continuous pretreatment of corn stover. The mean diameters of the pulverized biomasses were not significantly different in the three cases, and the glucose yields from the CBM-, ACM-, and HSM-pulverized samples were 29%, 49%, and 44%, respectively. The energy requirements and process capacities for the ACM and HSM were similar. We conclude that the ACM and HSM could be used in the continuous pretreatment of corn stover and would be useful in biofuel production.

ACS Style

Yang Mo Gu; Sunghyun Kim; Daekyung Sung; Byoung-In Sang; Jin Hyung Lee. Feasibility of Continuous Pretreatment of Corn Stover: A Comparison of Three Commercially Available Continuous Pulverizing Devices. Energies 2019, 12, 1422 .

AMA Style

Yang Mo Gu, Sunghyun Kim, Daekyung Sung, Byoung-In Sang, Jin Hyung Lee. Feasibility of Continuous Pretreatment of Corn Stover: A Comparison of Three Commercially Available Continuous Pulverizing Devices. Energies. 2019; 12 (8):1422.

Chicago/Turabian Style

Yang Mo Gu; Sunghyun Kim; Daekyung Sung; Byoung-In Sang; Jin Hyung Lee. 2019. "Feasibility of Continuous Pretreatment of Corn Stover: A Comparison of Three Commercially Available Continuous Pulverizing Devices." Energies 12, no. 8: 1422.

Journal article
Published: 16 September 2018 in Energies
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A combined ball milling and ethanol organosolv process is proposed for the pretreatment of three types of herbaceous biomass, giant miscanthus, corn stover, and wheat straw. The combined pretreatment was effective at both removing lignin and increasing the glucan content. After 120 min pretreatment, the glucan content increased to 63.09%, and 55.89% of the acid-insoluble lignin was removed from the giant miscanthus sample. The removal of cellulose, hemicellulose, and acetyl groups were correlated with the removal of lignin. The pretreatment of corn stover showed the highest removal of cellulose, but this was dependent on the removal of acid-insoluble lignin. The slope of the regression lines, which shows the correlation between the removal of lignin and cellulose, was lower than other correlations. The changes in biomass size were analyzed using size distribution graphs. With increasing pretreatment time, the particle size reduction improved in the three types of herbaceous biomass. Because of the combined physicochemical pretreatment, the enzymatic digestibility improved, and a maximum of 91% glucan digestibility was obtained from the pretreated corn stover when 30 FPU/g-glucan enzyme was added. Finally, compositional analysis of the recovered lignin from the remaining black liquor was investigated.

ACS Style

Seong Ju Kim; Byung Hwan Um; Dong Joong Im; Jin Hyung Lee; Kyeong Keun Oh. Combined Ball Milling and Ethanol Organosolv Pretreatment to Improve the Enzymatic Digestibility of Three Types of Herbaceous Biomass. Energies 2018, 11, 2457 .

AMA Style

Seong Ju Kim, Byung Hwan Um, Dong Joong Im, Jin Hyung Lee, Kyeong Keun Oh. Combined Ball Milling and Ethanol Organosolv Pretreatment to Improve the Enzymatic Digestibility of Three Types of Herbaceous Biomass. Energies. 2018; 11 (9):2457.

Chicago/Turabian Style

Seong Ju Kim; Byung Hwan Um; Dong Joong Im; Jin Hyung Lee; Kyeong Keun Oh. 2018. "Combined Ball Milling and Ethanol Organosolv Pretreatment to Improve the Enzymatic Digestibility of Three Types of Herbaceous Biomass." Energies 11, no. 9: 2457.

Journal article
Published: 01 June 2018 in Water-Energy Nexus
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ACS Style

Michael H. Kim; Joseph J. DeFrank; Jin Hyung Lee; Hyunook Kim. Continuous fed-batch alternating aerobic and anoxic system for biodegradation of Decontaminating Solution 2 with high nitrogen content. Water-Energy Nexus 2018, 1, 47 -55.

AMA Style

Michael H. Kim, Joseph J. DeFrank, Jin Hyung Lee, Hyunook Kim. Continuous fed-batch alternating aerobic and anoxic system for biodegradation of Decontaminating Solution 2 with high nitrogen content. Water-Energy Nexus. 2018; 1 (1):47-55.

Chicago/Turabian Style

Michael H. Kim; Joseph J. DeFrank; Jin Hyung Lee; Hyunook Kim. 2018. "Continuous fed-batch alternating aerobic and anoxic system for biodegradation of Decontaminating Solution 2 with high nitrogen content." Water-Energy Nexus 1, no. 1: 47-55.

Journal article
Published: 01 June 2018 in Water-Energy Nexus
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Lignocellulosic biomass pretreatment is a key step to determine the efficacy of biofuel production because of the recalcitrance of lignocellulosic feedstock. The effects of various water inputs, i.e., 0, 25, 100, and 400% (w/w), on the ball milling pretreatment of corn stover and enzymatic hydrolysis were studied under two milling temperatures (80°C and 100°C) and three milling times (10, 20, and 30 min). Ball milling reduced corn stover particle size and disrupted the rigid cell wall matrix. As milling time increased, corn stover size decreased remarkably. Changes in corn stover were analyzed using size distribution graphs and geometric mean diameter with respect to milling temperature and time. Milling with 0% (w/w) water resulted in the rapid grinding of corn stover. The highest glucose yield (66.96%) was obtained after milling at 80°C for 30 min with no water. The corn stovers milled with 0% or 25% (w/w) water showed higher glucose yields when milled at 80°C than at 100°C, while the samples milled with 100% or 400% (w/w) water showed higher glucose yields at 100°C. Finally, several ball milling conditions were proposed to construct a combinational pretreatment process based on the study results. These results provide a basis for minimizing water use during lignocellulosic biofuel production and improving sustainability.

ACS Style

Yang Mo Gu; Hyunook Kim; Byoung-In Sang; Jin Hyung Lee. Effects of water content on ball milling pretreatment and the enzymatic digestibility of corn stover. Water-Energy Nexus 2018, 1, 61 -65.

AMA Style

Yang Mo Gu, Hyunook Kim, Byoung-In Sang, Jin Hyung Lee. Effects of water content on ball milling pretreatment and the enzymatic digestibility of corn stover. Water-Energy Nexus. 2018; 1 (1):61-65.

Chicago/Turabian Style

Yang Mo Gu; Hyunook Kim; Byoung-In Sang; Jin Hyung Lee. 2018. "Effects of water content on ball milling pretreatment and the enzymatic digestibility of corn stover." Water-Energy Nexus 1, no. 1: 61-65.

Journal article
Published: 01 October 2017 in Bioresource Technology
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The impact of planetary mill pretreatment on corn stover size, crystallinity, and enzyme digestibility was investigated based on the grinding ball properties in an effort to reduce energy consumption. Alumina, zirconia, and steel balls were used, of which steel balls had the highest specific gravity but the lowest surface roughness while alumina balls had the lowest specific gravity and the highest roughness. The planetary mill pretreatment effectively reduced the size and crystallinity of the corn stover for all the ball types. Steel balls showed the highest size reduction effect in short milling time such as a 20min and alumina balls were effective at improving the surface area of the corn stover in a 60min milling time. The use of alumina balls resulted in the highest enzyme digestibility with a glucose yield of 91.9% when the milling time was 60min.

ACS Style

Jin Hyung Lee; Jeong Heo Kwon; Tae Hyun Kim; Won Il Choi. Impact of planetary ball mills on corn stover characteristics and enzymatic digestibility depending on grinding ball properties. Bioresource Technology 2017, 241, 1094 -1100.

AMA Style

Jin Hyung Lee, Jeong Heo Kwon, Tae Hyun Kim, Won Il Choi. Impact of planetary ball mills on corn stover characteristics and enzymatic digestibility depending on grinding ball properties. Bioresource Technology. 2017; 241 ():1094-1100.

Chicago/Turabian Style

Jin Hyung Lee; Jeong Heo Kwon; Tae Hyun Kim; Won Il Choi. 2017. "Impact of planetary ball mills on corn stover characteristics and enzymatic digestibility depending on grinding ball properties." Bioresource Technology 241, no. : 1094-1100.

Journal article
Published: 01 July 2017 in Carbon
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ACS Style

Joah Han; Jeong Heo Kwon; Jae-Won Lee; Jin Hyung Lee; Kwang Chul Roh. An effective approach to preparing partially graphitic activated carbon derived from structurally separated pitch pine biomass. Carbon 2017, 118, 431 -437.

AMA Style

Joah Han, Jeong Heo Kwon, Jae-Won Lee, Jin Hyung Lee, Kwang Chul Roh. An effective approach to preparing partially graphitic activated carbon derived from structurally separated pitch pine biomass. Carbon. 2017; 118 ():431-437.

Chicago/Turabian Style

Joah Han; Jeong Heo Kwon; Jae-Won Lee; Jin Hyung Lee; Kwang Chul Roh. 2017. "An effective approach to preparing partially graphitic activated carbon derived from structurally separated pitch pine biomass." Carbon 118, no. : 431-437.

Journal article
Published: 01 June 2017 in Journal of Industrial and Engineering Chemistry
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ACS Style

Jin Hyung Lee; Jeong Heo Kwon; Jae-Won Lee; Hye-Sun Lee; Jeong Ho Chang; Byoung-In Sang. Preparation of high purity silica originated from rice husks by chemically removing metallic impurities. Journal of Industrial and Engineering Chemistry 2017, 50, 79 -85.

AMA Style

Jin Hyung Lee, Jeong Heo Kwon, Jae-Won Lee, Hye-Sun Lee, Jeong Ho Chang, Byoung-In Sang. Preparation of high purity silica originated from rice husks by chemically removing metallic impurities. Journal of Industrial and Engineering Chemistry. 2017; 50 ():79-85.

Chicago/Turabian Style

Jin Hyung Lee; Jeong Heo Kwon; Jae-Won Lee; Hye-Sun Lee; Jeong Ho Chang; Byoung-In Sang. 2017. "Preparation of high purity silica originated from rice husks by chemically removing metallic impurities." Journal of Industrial and Engineering Chemistry 50, no. : 79-85.

Short communication
Published: 28 August 2015 in Bioresource Technology
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A facile butanol bioproduction process was developed using planetary milling, and Pinus rigida wood waste as a model substrate for fermentable sugars. The use of planetary milling as the pretreatment eliminates the need for washing and transfer of the biomass prior to enzymatic hydrolysis. Moreover, using this pretreatment process resulted in the production of only 0.072 ± 0.003 g/L soluble phenolic compounds, a concentration that was not inhibitory towards Clostridium beijerinckii NCIMB 8052. As the milling was performed in a compatible buffer (50 mM acetate, pH 4.8), the enzymatic hydrolysis step was initiated by simply adding the cellulase cocktail powder directly to pretreated biomass without washing the biomass or exchanging the buffer, resulting in a glucose yield of 31 g/L (84.02%). Fermentation of the hydrolysate samples by C. beijerinckii NCIMB 8052 gave slightly better butanol yields than cultures grown in a typical lab media (P2), with final concentrations of 6.91and 6.66 g/L, respectively.

ACS Style

Jeong Heo Kwon; Hyunsoo Kang; Byoung-In Sang; Yunje Kim; Jiho Min; Robert J. Mitchell; Jin Hyung Lee. Feasibility of a facile butanol bioproduction using planetary mill pretreatment. Bioresource Technology 2015, 199, 283 -287.

AMA Style

Jeong Heo Kwon, Hyunsoo Kang, Byoung-In Sang, Yunje Kim, Jiho Min, Robert J. Mitchell, Jin Hyung Lee. Feasibility of a facile butanol bioproduction using planetary mill pretreatment. Bioresource Technology. 2015; 199 ():283-287.

Chicago/Turabian Style

Jeong Heo Kwon; Hyunsoo Kang; Byoung-In Sang; Yunje Kim; Jiho Min; Robert J. Mitchell; Jin Hyung Lee. 2015. "Feasibility of a facile butanol bioproduction using planetary mill pretreatment." Bioresource Technology 199, no. : 283-287.

Journal article
Published: 16 April 2015 in Biotechnology for Biofuels
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Plant-based cellulose presents the best source of renewable sugars for biofuel production. However, the lignin associated with plant cellulose presents a hurdle as hydrolysis of this component leads to the production of inhibitory compounds, such as ferulic acid. The impacts of ferulic acid, a phenolic compound commonly found in lignin hydrolysates, on the growth, solvent production, and transcriptional responses of Clostridium beijerinckii NCIMB 8052 were determined. Addition of ferulic acid to growing cultures resulted in a decrease in the growth and solvent production by 30% and 25%, respectively, when compared to the control cultures. To better understand the toxicity of this compound, microarray analyses were performed using samples taken from these cultures at three different growth states. Several gene ontology terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified showing significant change at each status, including ATP-binding cassette (ABC) transporters, two component system, and oxidoreductase activity. Moreover, genes related with efflux systems and heat shock proteins were also strongly up-regulated. Among these, expression of the groESL operon was induced by more than fourfold and was consequently selected to improve C. beijerinckii tolerance to ferulic acid. Real-time quantitative PCR (RT-qPCR) analysis confirmed that C. beijerinckii harboring the plasmid, pSAAT-ptb_Gro, had a two- to fivefold increased groESL operon expression during growth of these cultures. Moreover, this strain was more tolerant to ferulic acid as the growth of this recombinant strain and its bioconversion of glucose into solvents were both improved. Using transcriptomics, we identified numerous genes that are differentially expressed when C. beijerinckii cultures were exposed to ferulic acid for varying amounts of time. The operon expressing groESL was consistently up-regulated, suggesting that this gene cluster may contribute to strain tolerance. This was confirmed as recombinant cultures showed both an enhanced growth and solvent yield in the presence of 0.5 g/L ferulic acid.

ACS Style

Siseon Lee; Jin Hyung Lee; Robert J. Mitchell. Analysis of Clostridium beijerinckii NCIMB 8052’s transcriptional response to ferulic acid and its application to enhance the strain tolerance. Biotechnology for Biofuels 2015, 8, 1 -14.

AMA Style

Siseon Lee, Jin Hyung Lee, Robert J. Mitchell. Analysis of Clostridium beijerinckii NCIMB 8052’s transcriptional response to ferulic acid and its application to enhance the strain tolerance. Biotechnology for Biofuels. 2015; 8 (1):1-14.

Chicago/Turabian Style

Siseon Lee; Jin Hyung Lee; Robert J. Mitchell. 2015. "Analysis of Clostridium beijerinckii NCIMB 8052’s transcriptional response to ferulic acid and its application to enhance the strain tolerance." Biotechnology for Biofuels 8, no. 1: 1-14.

Journal article
Published: 01 November 2013 in International Journal of Hydrogen Energy
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RT-qPCR data demonstrated that the yhcN gene was highly expressed when Escherichia coli was exposed to 500 ppm of ferulic acid (9.8-fold), vanillin (7.3-fold) or furfural (2.2-fold). Consequently, an E. coli yhcN::lux bioreporter strain (DMY1) was constructed. This strain is very responsive to furfural and 5-hydroxymethylfurfural, showing a maximum induced bioluminescence of 29.4- and 17.8-fold, respectively. The responses to different phenolics were also significant, with relative responses of between 4- and 16-fold. In tests with rice straw hydrolysate samples, a dual induction of DMY1 was observed and it was demonstrated that this corresponded to the activities of the phenolics and HMF within the sample. The maximum response seen with the hydrolysate sample was 3.5 and the minimum phenolic concentration detected was 39 mg/L. These results illustrate that this strain can be used to monitor for the presence of furans and phenolics commonly found within plant hydrolysate samples.close

ACS Style

Ajay Kalanjana Monnappa; Jin Hyung Lee; Robert Mitchell. Detection of furfural and 5-hydroxymethylfurfural with a yhcN::luxCDABE bioreporter strain. International Journal of Hydrogen Energy 2013, 38, 15738 -15743.

AMA Style

Ajay Kalanjana Monnappa, Jin Hyung Lee, Robert Mitchell. Detection of furfural and 5-hydroxymethylfurfural with a yhcN::luxCDABE bioreporter strain. International Journal of Hydrogen Energy. 2013; 38 (35):15738-15743.

Chicago/Turabian Style

Ajay Kalanjana Monnappa; Jin Hyung Lee; Robert Mitchell. 2013. "Detection of furfural and 5-hydroxymethylfurfural with a yhcN::luxCDABE bioreporter strain." International Journal of Hydrogen Energy 38, no. 35: 15738-15743.

Nano express
Published: 01 January 2012 in Nanoscale Research Letters
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Thiol group functionalized silica-coated magnetic nanoparticles ([email protected]) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by [email protected] was governed not only by pH but also by the molecular weight of each protein in the mixed protein.

ACS Style

Soo Youn Lee; Chi Young Ahn; Jiho Lee; Jin Hyung Lee; Jeong Ho Chang. Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles. Nanoscale Research Letters 2012, 7, 279 -279.

AMA Style

Soo Youn Lee, Chi Young Ahn, Jiho Lee, Jin Hyung Lee, Jeong Ho Chang. Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles. Nanoscale Research Letters. 2012; 7 (1):279-279.

Chicago/Turabian Style

Soo Youn Lee; Chi Young Ahn; Jiho Lee; Jin Hyung Lee; Jeong Ho Chang. 2012. "Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles." Nanoscale Research Letters 7, no. 1: 279-279.