Biodegradable composite membranes containing propolis were produced from PCL/PLLA blends using a simple and low-cost solvent casting method, and subsequently their physicochemical, mechanical, and antibacterial properties were characterized. SEM analysis revealed that the addition of propolis has created honeycomb-like structures on the film surfaces. The flexibility of the films increased in the presence of propolis, which may provide ease of use during application. Propolis disrupted the organized structure of both polymers at the molecular level and caused decreases in the melting points. The films with propolis showed faster degradation in physiological conditions due to this molecular disruption. Moreover, the PLLA/PCL/propolis composite films exhibited remarkable antibacterial activities againstS. aureus. Collectively, the data suggest that the produced films might be used as an alternative to exiting barrier membranes in guided tissue regeneration.

Spinal cord injury is a devastating condition of the central nervous system, in which traditional treatments are largely ineffective due to the complex nature of the injured tissue. Therefore, biomaterial-based systems have been developed as possible alternative strategies to repair the damaged tissue. In the present study, we aimed to design bioactive agent loaded scaffolds composed of two layers with distinct physical properties to improve tissue regeneration. An electrospun layer with aligned nanofibers was made of collagen (Col) Type-I, poly(lactide-co-glycolide) (PLGA) and laminin to promote cell attachment of mesenchymal-like stem cells towards the direction of fibers, while a Col-based second layer was fabricated by plastic compression to act as a releasing system for NT-3 and chondroitinase ABC, so that axonal growth could be stimulated. Results showed that a source of mesenchymal stem cell (MSC)-like cells, adipose tissue-derived stem cells cultured on the fibrous layer of the matrices were able to adhere and proliferate, where the aligned fibers promoted the cell growth in an organized way. Furthermore, the bilayered matrices also promoted dorsal root ganglion neurite outgrowth. The bilayered matrice with Col/PLGA + laminin top layer appears to promote higher neurite growth. Collectively, the designed constructs show promising structural properties and biological performance for being employed as a scaffold for engineering the spinal cord tissue.

Poly(lactic acid) (PLA)-based biocomposites incorporating collagen (COLL) and hydroxyapatite (HA) were produced via melt micro-compounding and subsequent injection molding. 1,4-phenylene diisocyanate (PDI) was employed as a compatibilizer, while poly(ethylene glycol) (PEG) was used as a plasticizer. The morphological, thermal, rheological, and mechanical properties, as well as surface wettability, degradation behavior, and cytotoxicity, were comprehensively evaluated. SEM and DSC analyses revealed the phase distribution and thermal transitions, while rheological measurements showed that PEG reduced melt viscosity by increasing chain mobility. Mechanical performance was evaluated using tensile, impact, and DMA tests on standard specimens, indicating that HA primarily enhanced stiffness (elastic modulus), whereas PEG improved toughness, resulting in higher impact strength. Biodegradable bone screw prototypes were produced with the same formulations and subjected to torsion, enzymatic degradation, and MTT cytotoxicity tests. Degradation results indicated that biocomposites containing PEG, collagen, and HA exhibited accelerated mass loss. Overall, the 70/20/10 PLA/COLL/HA/PEG/PDI formulation was more suitable for soft (trabecular) bone tissue, while the 70/10/20 PLA/COLL/HA/PDI formulation showed advantages for hard (cortical) bone tissue applications.