In order to determine ileal losses of nitrogen (N) and amino acids (AA) and the coefficients of apparent and true ileal digestibility (AID, TID) of N and AA from casein in piglets and pigs, two experiments were conducted. In Experiment 1, 24 piglets were used. The piglets were weaned at 17 days of age, weighing 6.4 kg and cannulated at terminal ileum. Ileal digesta was collected at 28 – 29 and 35 – 36 days of age in period 1 and 2, respectively. Feed intake was 150 and 300 g · d⁻¹ during the first and second period. In Experiment 2, 16 castrates weighing 52.5 kg and cannulated at terminal ileum were used. The intake level of digestible energy was 2.5 times their maintenance requirement. The experiment lasted 7 days and ileal digesta was collected on day 6 – 7. Treatments consisted of four levels of N from casein: 8, 16, 24 and 32 g N · kg⁻¹ feed, respectively. Results showed that N level did not increase N or AA ileal losses. In piglets, N and AA ileal losses were similar between periods, except for period 2, where losses per kg DMI were about 47 and 64% higher for glycine and proline, respectively (p < 0.05). When ileal losses from pigs and piglets were compared, piglets had higher (p < 0.05) ileal losses of N and AA (excepted glutamic acid and alanine). A lower (p < 0.05) AID was observed in piglets in period 2 for N, methionine, glutamic acid, glycine and proline. With exception of glycine in pigs, all values for TID of N and AA of casein were superior to 0.90. Piglets had higher TID of N, leucine, isoleucine, valine and phenylalanine. These results showed that piglets have higher ileal losses than pigs.

Background: Many studies have investigated endogenous loss of proteins and amino acids (AAs) at the ileal level in growing pigs. However, only a few studies have researched this subject in piglets. Knowledge regarding AA ileal digestibility in piglets would be helpful during the formulation of diets for weaning piglets, rather than just using coefficients obtained in growing pigs. Therefore, in this study, we sought to estimate endogenous protein and AA ileal losses in piglets. Furthermore, apparent and true ileal digestibility (AID and TID) of protein and AAs from casein were measured. Results: The average flow of protein was 20.8 g/kg of dry matter intake (DMI). Basal protein loss, as estimated by regression, was 16.9 g/kg DMI. Glutamic acid, arginine, and aspartic acid (2.2, 1.4, and 1.2 g/kg DMI, respectively) were the AAs for which greater losses were seen. The AID of protein and AAs increased as the protein level in the diet increased. A higher increment in AID was observed between diets with 80 and160 g CP/kg of feed; this finding was mainly attributable to increases in glycine and arginine (46.1% and 18%, respectively). The TID of protein was 97.8, and the TID of AAs varied from 93.9 for histidine to 100.2 for phenylalanine. Conclusions: The basal endogenous protein loss in piglets was 16.9 g/kg DMI. Endogenous protein was rich in glutamic acid, aspartic acid, and arginine, which represented 32.7% of endogenous protein loss in weaning piglets. The TID of casein was high and varied from 93.0 for histidine to 100.2 for phenylalanine.

The effects of kafirins on protein and amino acid ileal digestibility have not been evaluated in vivo in pigs. The aim of this study was to determine the effects of protein profile on apparent ileal digestibility (AID) of amino acids. We used a sorghum hybrid with low tannin content (<0.5%). The same hybrid was harvested from 2 different plots with different kafirin profile. Sorghum with greater content of total kafirins had less content of γ- and α1-kafirins and higher content of β- and α2-kafirins than that with lower content of total kafirins. Two sorghum-soybean meal (SBM) diets were formulated: 1) low kafirin (LK) content (32.2 g/kg) and 2) high kafirin (HK) content (48.1 g/kg). A control diet (maize-SBM) and a reference SBM-diet were also prepared. The reference diet was fed to all pigs following the experimental period and was used to estimate the AID of cereals by the difference method. "T" cannulas were fixed in the distal ileum of 18 barrows (6 by treatment), divided into 2 groups of 9 pigs. The pigs were fed 2.5 times their maintenance requirement of digestible energy (110 kcal/kg BW^0.75). The AID of dry matter, protein, amino acids, and energy of the experimental diets was measured; the AID of cereals (maize, LK sorghum and HK sorghum) was estimated by the difference method. The maize-SBM diet was more digestible than the sorghum-SBM diets, only with respect to valine (P < 0.05). The AID of valine in the maize-SBM diet was higher than that in sorghum-SBM diets. The changes in kafirin profile between the diets only affected the AID of threonine (P < 0.01), which decreased by 9.5 percentage units in LK diet compared with HK diet. Regarding the AID of cereals, maize exhibited greater AID than sorghum, with respect to valine (P < 0.01) and serine (P < 0.10). A comparison of sorghum with LK and HK content showed that the AID of threonine and serine increased by 50.5 (P < 0.001) and 19.2 percentage units (P < 0.05) in the latter, respectively. The higher content of γ-kafirins in LK sorghum negatively affected threonine and serine digestibility, implying that the AID of amino acids is affected more by the profile than the content of kafirins.

Two experiments were conducted to test if dehydrated porcine plasma (DPP) and potato protein concentrate (PPC) could be used as an alternative to antibiotics in starter diets for piglets. Experiment one was conducted to test if DPP and PPC in an antibiotic-free diet affected pig performance, and faecal consistency. Eighty-four piglets weaned at 22 days and weighing 6.9 kg were used. Piglets were fed for two weeks with one of four diets: a positive control diet with antibiotics (C+); and three other diets without antibiotics added with DPP, PPC, or DPP and PPC (DPP+PPC) to measure the average daily feed intake (ADFI), average daily gain (ADG), feed efficiency (FE), and incidence and severity of diarrhoea (ID and ISD respectively). In experiment two, twenty-four piglets weaned at 17 days and weighing 5.7 kg, were implanted at 21 days of age with a T-cannula at the terminal ileum to measure the apparent ileal digestibility (AID) and the apparent total tract digestibility (ATTD) of nutrients. Piglets were fed one of four diets: a positive control diet with antibiotics (C+); a negative control diet without antibiotics (C–), and two diets without antibiotics added with DPP, or PPC. The results of experiment one showed that the DPP diet was the most consumed diet during the first week, and the ADG and FE were similar among treatments. During the second week and the total experimental period the ADFI, ADG, and FE were similar among diets. The ID was lower in the C+ diet than other diets. The ISD was lower in the C+ diet than DPP and DPP+PPC diets; piglets fed PPC diet were similar to piglets fed C+ and DPP and DPP+PPC diets. The results of digestibility showed that crude protein AID was higher in piglets fed C+ and PPC diets than C– and DPP diets. Dry matter ATTD and energy ATTD were higher for piglets fed PPC than other diets. Further, crude protein ATTD of DPP and PPC diets tended to have a similar digestibility to that of C+ diet. The results suggest that PPC is a potential controller of post-weaning diarrhoea.