Epizootic rabbit enteropathy (ERE) affects young rabbits and represents 32% of the enteropathies in rabbit production farms in Mexico. The etiology of this syndrome has not been clarified yet. A metataxonomic and histopathology study of ERE was carried out to compare the gastrointestinal microbiota and histopathological lesions of healthy and positive-ERE rabbits. The metataxonomic study was done using an Illumina MiSeq (MiSeq^® system, Illumina, San Diego California, USA) massive segmentation platform, and a Divisive Amplicon Denoising Algorithm 2 (DADA2 algorithm) was used to obtain Shannon and Simpson diversity indices as well as the relative abundance of the identified communities. For the histopathological study, paraffin sections of the cecum, ileo-cecal valve, and colon were stained with eosin and hematoxylin. AxioVision 4.9 software (Carl Zeiss MicroImaging GmbH, Jena, Germany) was used to measure the crypt depths. Statistical analysis was done using PERMANOVA analysis for the metataxonomic study and ANOVA for the histopathology study. Histopathologic analysis showed smaller sizes of crypts in the colon of ERE rabbits. Differences were observed in the diversity and abundance of the gastrointestinal microbiota between the analyzed groups. The genus Clostridium and the species Cloacibacillus porcorum and Akkermansia muciniphila were associated with ERE. The results obtained from this study can provide information for future clarification of the etiology and proposals of effective treatments.

The effects of kafirins on protein and amino acid ileal digestibility have not been evaluated in vivo in pigs. The aim of this study was to determine the effects of protein profile on apparent ileal digestibility (AID) of amino acids. We used a sorghum hybrid with low tannin content (<0.5%). The same hybrid was harvested from 2 different plots with different kafirin profile. Sorghum with greater content of total kafirins had less content of γ- and α1-kafirins and higher content of β- and α2-kafirins than that with lower content of total kafirins. Two sorghum-soybean meal (SBM) diets were formulated: 1) low kafirin (LK) content (32.2 g/kg) and 2) high kafirin (HK) content (48.1 g/kg). A control diet (maize-SBM) and a reference SBM-diet were also prepared. The reference diet was fed to all pigs following the experimental period and was used to estimate the AID of cereals by the difference method. "T" cannulas were fixed in the distal ileum of 18 barrows (6 by treatment), divided into 2 groups of 9 pigs. The pigs were fed 2.5 times their maintenance requirement of digestible energy (110 kcal/kg BW^0.75). The AID of dry matter, protein, amino acids, and energy of the experimental diets was measured; the AID of cereals (maize, LK sorghum and HK sorghum) was estimated by the difference method. The maize-SBM diet was more digestible than the sorghum-SBM diets, only with respect to valine (P < 0.05). The AID of valine in the maize-SBM diet was higher than that in sorghum-SBM diets. The changes in kafirin profile between the diets only affected the AID of threonine (P < 0.01), which decreased by 9.5 percentage units in LK diet compared with HK diet. Regarding the AID of cereals, maize exhibited greater AID than sorghum, with respect to valine (P < 0.01) and serine (P < 0.10). A comparison of sorghum with LK and HK content showed that the AID of threonine and serine increased by 50.5 (P < 0.001) and 19.2 percentage units (P < 0.05) in the latter, respectively. The higher content of γ-kafirins in LK sorghum negatively affected threonine and serine digestibility, implying that the AID of amino acids is affected more by the profile than the content of kafirins.

Context: Tannins are blamed for negatively affecting nutritive value of sorghum. Kafirins are sorghum storage proteins and may be associated with lower animal performance. They have been tested in poultry diets; however, information for pig diets is scarce. Aims: Our aim was to determine the role of kafirins and tannins in sorghum nutritive value for swine. Methods: Two experiments evaluated effects of sorghum tannin and kafirin levels on (1) nitrogen (N) and energy balance, and (2) performance of pigs. Two sorghum hybrids with low (LT) and high (HT) tannins were grown at two rates of N fertiliser to achieve low (LK) and high (HK) kafirins (i.e. four sorghum treatments: LTLK, LTHK, HTLK and HTHK) and added to soybean meal based diets. A fifth diet included maize instead of sorghum. Five pigs (48.8 ± 0.4 kg) were used in a 5 × 5 Latin square design to measure N and energy balance, with 5 days of diet adaptation and 4 days of urine and faeces collection. The performance experiment included 60 pigs (54.0 ± 12.4 kg, 30 barrows and 30 gilts, six of each per treatment) placed in individual pens with free access to water and feed. Key results: Energy balance showed a tannin × kafirin interaction (P < 0.05) for dry matter feed intake, which diminished with the LTHK diet and was unaffected with the HTHK diet. Dry matter digestibility was higher (P < 0.001) with maize, intermediate with LT, and lower with HT diets. Pigs fed maize retained more N (P < 0.05) and had higher energy digestibility (P < 0.001) than pigs fed sorghum. HT diets had lower (P < 0.01) energy digestibility than LT diets. In the finishing phase, average daily feed intake showed a tannin × kafirin interaction (P < 0.05); pigs fed the HTHK diet had lower average daily feed intake than other pigs. This lowered average daily liveweight gain and consequently final weight. Conclusions: Tannins negatively affected sorghum nutritive value; high kafirins worsened average daily feed intake and liveweight gain, and hence final weight. Implications: Management of kafirin content should be considered to avoid adverse performance effects in pigs fed sorghum.

Many countries have banned the use of antibiotics in livestock feed due to the development of antibiotic-resistant bacteria. Therefore, it is necessary to identify suitable alternatives to antibiotics in order to maintain intestinal health and improve piglet performance after weaning. The objective of this study was to evaluate how the intake of two functional foods—potato protein concentrate (PP) and Saccharomyces boulardii (Sb), either isolated or combined—would affect productive performance and some digestive morphophysiological responses after two weeks post-weaning. We evaluated five diets: SB, with Sb; PPC, with PP; PPC-SB, with a combination of PP and Sb; C−, without antibiotics, Sb, or PP; and C+, with antibiotics. Neither the absence of antibiotics in the diet nor the inclusion of PP and Sb affected performance or digestive organ development during the post-weaning phase. Piglets fed the C+ diet and the PP-Sb combination had higher concentrations of occludin proteins and longer villi in the jejunum compared to those fed C−, PPC, and SB. The use of Sb alone or in combination with PP increased fecal Lactobacillus, while antibiotics reduced coliform bacteria. The PP-Sb combination prevented the atrophy of the jejunal villi and promoted adhesion between enterocytes in the jejunum. Including these functional foods in piglet diets contributed to maintaining intestinal health during the critical post-weaning phase. However, further research is needed to validate and enhance the conclusions of this study.

Hospital-Associated Infections (HAIs) are caused by microorganisms that are not present before patients are admitted to healthcare facilities, and usually have multidrug resistance profiles. There is ample information and active research in human medicine to create preventive and control measures, but there have been fewer efforts in animal medicine, and studies in only a few countries have been examining how this problem presents in veterinary hospitals. In Mexico, there have been no studies on the presence of multidrug-resistant bacteria associated with HAIs in veterinary medicine. Therefore, the surfaces of inanimate objects and equipment in a university veterinary hospital for small species were sampled to search for bacteria with the potential to cause HAIs. After isolation, molecular identification and multidrug resistance tests were carried out. One bacterial strain was found to be resistant to carbapenems, third-generation cephalosporines, and penicillin/β-lactamase inhibitors. Additionally, other susceptible bacterial genera were identified as potential nosocomial pathogens in humans and animals. The presence of multidrug-resistant bacteria was confirmed. Further studies should be conducted to determine the isolate’s origin and its relationship with reported human clinical genotypes. This type of study highlights the importance of epidemiological surveillance and the need to not underestimate the potential risk posed by multidrug-resistant microorganisms.

Many countries have banned the use of antibiotics in livestock feed owing to the develop-ment of antibiotic-resistant bacteria. Therefore, it is necessary to identify suitable alterna-tives to antibiotics to maintain intestinal health and improve piglet performance after the weaning. The objective of the present research was to evaluate the effect of isolated or combined intake of potato protein concentrate (PPC) and Saccharomyces boulardii (SB) on productive performance and some morphophysiological responses of the digestive tract in the two post-weaning weeks. We probe negative control without antibiotics, PPC, or SB; positive control with only antibiotics, and three other diets with SB, PPC, or a combination of PPC-SB. The absence of antibiotics in the diet or the inclusion of PPC and SB did not af-fect performance and digestive organ development after the weaning. The piglets fed a combination of PPC-SB had a higher concentration of protein occludins in the jejunum and intestinal villi than those fed a C- diet and had a similar effect to the C+ diet. The use of SB alone or in combination with PPC increased fecal Lactobacillus and antibiotics re-duced coliform bacteria. The PPC-SB combination prevented atrophy of the jejunal villi and promoted adhesion between enterocytes in the jejunum.