Soil greenhouse gas (GHG) fluxes relate to soil carbon and nitrogen budgets and have a significant impact on climate change. Nevertheless, the temporal variation and magnitude of the fluxes of all three major GHGs (CO₂, CH₄ and N₂O) and their influencing factors have not been elucidated clearly in primary forests on the eastern Qinghai-Tibetan Plateau. Herein, field chamber GHG fluxes from May to November, soil microbial community and enzyme activity were analyzed in a fir-dominated (Abies fargesii var. faxoniana) primary forest. The emission rates of CO₂ and N₂O ranged between 64.69–243.22 mg CO₂ m⁻² h⁻¹ and 1.69–5.46 ug N₂O m⁻² h⁻¹, exhibiting a temporally unimodal pattern with a peak in July. The soil acted as a CH₄ sink, and the uptake rate varied between 52.96 and 84.67 μg CH₄ m⁻² h⁻¹ with the higher uptake rates in June and November. The temporal variation in the CO₂ flux was significantly correlated with the geometric mean of enzyme activities, suggesting that the soil CO₂ flux was determined by microbial activity rather than soil microbial biomass. The soil N₂O flux was positively related to nitrate concentration with marginal significance, probably because N₂O was a byproduct of nitrification and denitrification processes. The soil CH₄ uptake was closely associated with methanotrophic biomass (18:1ω7c). The results highlight divergent temporal dynamics of GHG fluxes owing to different driving mechanisms and an important CH₄ sink in the primary forest soil, helping to evaluate the carbon and nitrogen budgets of primary forests on the eastern Qinghai-Tibetan Plateau.

Morphological plasticity (MP) is an essential strategy for plants in nutrient acquisition, disturbance alleviation, and community coexistence during environmental and climatic changes. However, to date, there has been little research concerning the MP for alpine–subalpine forests on the Qinghai–Tibet plateau. These forests are representative of the ectomycorrhizal (ECM) type, and morphological traits of these ECM roots, such as root tip lengths, diameters, and their adherent hyphal lengths and exploration types, have rarely been studied in the context of nutrient and environmental gradients. In this study, we examined the morphological traits of ECM roots for faxon fir (Abies fargesii var. faxoniana), which dominated in subalpine forests across nine elevations on the Eastern Qinghai–Tibet plateau. By quantifying ca. 90,000 root tips, the hyphal lengths of ectomycorrhizal extraradical mycelium (EEM, i.e., short- and long-distance exploration types) reached up to 1.1 × 10⁶ cm/m³ in soil, which decreased significantly due to gradually increasing altitude. In contrast, the variability of ECM root traits (diameter, length, and superficial area) was highly conserved along the altitudinal gradients, yet the root tip lengths were positively associated with soil protease enzyme activity. The increase in diameter and length of ECM root tips was climate-independent yet significantly associated with increasing root N concentration. In the studied forests, a long-distance exploration type of ECM hyphae was controlled by precipitation (p < 0.05), whereas the short-distance one was controlled by precipitation and temperature simultaneously. The EEM lengths of short- and long-distance exploration types were associated with high C concentration and low N concentration in host tree root tissues. Our findings demonstrated that MP expression in nutrient-foraging strategies for the dominant coniferous trees facilitates the adaptation to changing environments by specialized hyphal structures. In conclusion, ECM root tips and hyphal structures are two dimensions of functional traits linked to root N concentration in opposite ways, and their MP collectively ensures the temporal stability and resistance of subalpine forests on the Qinghai–Tibet plateau. These results provide new insights into ECM morphological traits and their adaptation in changing environments, which is valuable for understanding responses of subalpine forests to climate change.