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Aldo Barreiro Felpeto
CIIMAR/CIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos, 4450-208 Matosinhos, Portugal

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Journal article
Published: 30 July 2020 in Biomolecules
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The development of alternative ecological and effective antifouling technologies is still challenging. Synthesis of nature-inspired compounds has been exploited, given the potential to assure commercial supplies of potential ecofriendly antifouling agents. In this direction, the antifouling activity of a series of nineteen synthetic small molecules, with chemical similarities with natural products, were exploited in this work. Six (4, 5, 7, 10, 15 and 17) of the tested xanthones showed in vivo activity toward the settlement of Mytilus galloprovincialis larvae (EC50: 3.53–28.60 µM) and low toxicity to this macrofouling species (LC50 > 500 µM and LC50/EC50: 17.42–141.64), and two of them (7 and 10) showed no general marine ecotoxicity (Artemia salina mortality) after 48 h of exposure. Regarding the mechanism of action in mussel larvae, the best performance compounds 4 and 5 might be acting by the inhibition of acetylcholinesterase activity (in vitro and in silico studies), while 7 and 10 showed specific targets (proteomic studies) directly related with the mussel adhesive structure (byssal threads), given by the alterations in the expression of Mytilus collagen proteins (PreCols) and proximal thread proteins (TMPs). A quantitative structure-activity relationship (QSAR) model was built with predictive capacity to enable speeding the design of new potential active compounds.

ACS Style

Joana Almeida; Andreia Palmeira; Alexandre Campos; Isabel Cunha; Micaela Freitas; Aldo Felpeto; Maria Turkina; Vitor Vasconcelos; Madalena Pinto; Marta Correia-Da-Silva; Maria Emília Sousa. Structure-Antifouling Activity Relationship and Molecular Targets of Bio-Inspired(thio)xanthones. Biomolecules 2020, 10, 1126 .

AMA Style

Joana Almeida, Andreia Palmeira, Alexandre Campos, Isabel Cunha, Micaela Freitas, Aldo Felpeto, Maria Turkina, Vitor Vasconcelos, Madalena Pinto, Marta Correia-Da-Silva, Maria Emília Sousa. Structure-Antifouling Activity Relationship and Molecular Targets of Bio-Inspired(thio)xanthones. Biomolecules. 2020; 10 (8):1126.

Chicago/Turabian Style

Joana Almeida; Andreia Palmeira; Alexandre Campos; Isabel Cunha; Micaela Freitas; Aldo Felpeto; Maria Turkina; Vitor Vasconcelos; Madalena Pinto; Marta Correia-Da-Silva; Maria Emília Sousa. 2020. "Structure-Antifouling Activity Relationship and Molecular Targets of Bio-Inspired(thio)xanthones." Biomolecules 10, no. 8: 1126.

Journal article
Published: 27 March 2020 in Marine Drugs
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Organisms belonging to Synechococcus sp. genera are observed in all freshwater, brackish, and marine waters of the world. They play a relevant role in these ecosystems, since they are one of the main primary producers, especially in open ocean. Eventually, they form mass blooms in coastal areas, which are potentially dangerous for the functioning of marine ecosystems. Allelopathy could be an important factor promoting the proliferation of these organisms. According to the authors’ best knowledge, there is no information on the allelopathic activity and allelopathic compounds exhibited by different Synechococcus sp. phenotypes. Therefore, the research conducted here aimed to study the bioactivity of compounds produced by three phenotypes of Synechococcus sp. by studying their influence on the growth, chlorophyll fluorescence, and photosynthetic pigments of eighteen cyanobacteria and microalgae species. We demonstrated that three different Synechococcus sp. phenotypes, including a phycocyanin (PC)-rich strain (Type 1; green strain) and phycoerythrin (PE)-rich strains containing phycoerythrobilin (PEB) and phycocyanobilin (PCB) (Type 2; red strain and Type 3a; brown strain), had a significant allelopathic effect on the selected species of cyanobacteria, diatoms, and green algae. For all green algae, a decrease in cell abundance under the influence of phenotypes of donor cyanobacteria was shown, whereas, among some target cyanobacteria and diatom species, the cell-free filtrate was observed to have a stimulatory effect. Our estimates of the stress on photosystem II (Fv/Fm) showed a similar pattern, although for some diatoms, there was an effect of stress on photosynthesis, while a stimulatory effect on growth was also displayed. The pigment content was affected by allelopathy in most cases, particularly for chlorophyll a, whilst it was a bit less significant for carotenoids. Our results showed that Synechococcus sp. Type 3a had the strongest effect on target species, while Synechococcus sp. Type 1 had the weakest allelopathic effect. Furthermore, GC-MS analysis produced different biochemical profiles for the Synechococcus strains. For every phenotype, the most abundant compound was different, with oxime-, methoxy-phenyl- being the most abundant substance for Synechococcus Type 1, eicosane for Synechococcus Type 2, and silanediol for Synechococcus Type 3a.

ACS Style

Zofia Konarzewska; Sylwia Śliwińska-Wilczewska; Aldo Barreiro Felpeto; Vitor Vasconcelos; Adam Latała. Assessment of the Allelochemical Activity and Biochemical Profile of Different Phenotypes of Picocyanobacteria from the Genus Synechococcus. Marine Drugs 2020, 18, 179 .

AMA Style

Zofia Konarzewska, Sylwia Śliwińska-Wilczewska, Aldo Barreiro Felpeto, Vitor Vasconcelos, Adam Latała. Assessment of the Allelochemical Activity and Biochemical Profile of Different Phenotypes of Picocyanobacteria from the Genus Synechococcus. Marine Drugs. 2020; 18 (4):179.

Chicago/Turabian Style

Zofia Konarzewska; Sylwia Śliwińska-Wilczewska; Aldo Barreiro Felpeto; Vitor Vasconcelos; Adam Latała. 2020. "Assessment of the Allelochemical Activity and Biochemical Profile of Different Phenotypes of Picocyanobacteria from the Genus Synechococcus." Marine Drugs 18, no. 4: 179.

Journal article
Published: 20 March 2020 in Toxins
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Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.

ACS Style

Flavio Oliveira; Leticia Diez-Quijada; Maria V. Turkina; João Morais; Aldo Barreiro Felpeto; Joana Azevedo; Angeles Jos; Ana M. Camean; Vitor Vasconcelos; José Carlos Martins; Alexandre Campos. Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum. Toxins 2020, 12, 196 .

AMA Style

Flavio Oliveira, Leticia Diez-Quijada, Maria V. Turkina, João Morais, Aldo Barreiro Felpeto, Joana Azevedo, Angeles Jos, Ana M. Camean, Vitor Vasconcelos, José Carlos Martins, Alexandre Campos. Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum. Toxins. 2020; 12 (3):196.

Chicago/Turabian Style

Flavio Oliveira; Leticia Diez-Quijada; Maria V. Turkina; João Morais; Aldo Barreiro Felpeto; Joana Azevedo; Angeles Jos; Ana M. Camean; Vitor Vasconcelos; José Carlos Martins; Alexandre Campos. 2020. "Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum." Toxins 12, no. 3: 196.

Journal article
Published: 06 December 2019 in Toxins
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Only a few studies have documented the physiological effects of allelopathy from cyanobacteria against coexisting microalgae. We investigated the allelopathic ability of the bloom-forming cyanobacteria Synechococcus sp. and Nodularia spumigena filtrates on several aspects related to the physiology of the target species: population growth, cell morphology, and several indexes of photosynthesis rate and respiration. The target species were the following: two species of green algae (Oocystis submarina, Chlorella vulgaris) and two species of diatoms (Bacillaria paxillifer, Skeletonema marinoi). These four species coexist in the natural environment with the employed strains of Synechococcus sp. and N. spumigena employed. The tests were performed with single and repeated addition of cyanobacterial cell-free filtrate. We also tested the importance of the growth phase in the strength of the allelopathic effect. The negative effects of both cyanobacteria were the strongest with repeated exudates addition, and generally, Synechococcus sp. and N. spumigena were allelopathic only in the exponential growth phase. O. submarina was not negatively affected by Synechococcus filtrates in any of the parameters studied, while C. vulgaris, B. paxillifer, and S. marinoi were affected in several ways. N. spumigena was characterized by a stronger allelopathic activity than Synechococcus sp., showing a negative effect on all target species. The highest decline in growth, as well as the most apparent cell physical damage, was observed for the diatom S. marinoi. Our findings suggest that cyanobacterial allelochemicals are associated with the cell physical damage, as well as a reduced performance in respiration and photosynthesis system in the studied microalgae which cause the inhibition of the population growth. Moreover, our study has shown that some biotic factors that increase the intensity of allelopathic effects may also alter the ratio between bloom-forming cyanobacteria and some phytoplankton species that occur in the same aquatic ecosystem.

ACS Style

Sylwia Śliwińska-Wilczewska; Aldo Barreiro Felpeto; Katarzyna Możdżeń; Vitor Vasconcelos; Adam Latała. Physiological Effects on Coexisting Microalgae of the Allelochemicals Produced by the Bloom-Forming Cyanobacteria Synechococcus sp. and Nodularia Spumigena. Toxins 2019, 11, 712 .

AMA Style

Sylwia Śliwińska-Wilczewska, Aldo Barreiro Felpeto, Katarzyna Możdżeń, Vitor Vasconcelos, Adam Latała. Physiological Effects on Coexisting Microalgae of the Allelochemicals Produced by the Bloom-Forming Cyanobacteria Synechococcus sp. and Nodularia Spumigena. Toxins. 2019; 11 (12):712.

Chicago/Turabian Style

Sylwia Śliwińska-Wilczewska; Aldo Barreiro Felpeto; Katarzyna Możdżeń; Vitor Vasconcelos; Adam Latała. 2019. "Physiological Effects on Coexisting Microalgae of the Allelochemicals Produced by the Bloom-Forming Cyanobacteria Synechococcus sp. and Nodularia Spumigena." Toxins 11, no. 12: 712.

Journal article
Published: 28 October 2019 in Toxins
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Cyanobacteria and cyanotoxins constitute a serious environmental and human health problem. Moreover, concerns are raised with the use of contaminated water in agriculture and vegetable production as this can lead to food contamination and human exposure to toxins as well as impairment in crop development and productivity. The objective of this work was to assess the susceptibility of two green vegetables, spinach and lettuce, to the cyanotoxins microcystin (MC) and cylindrospermopsin (CYN), individually and in mixture. The study consisted of growing both vegetables in hydroponics, under controlled conditions, for 21 days in nutrient medium doped with MC or CYN at 10 μg/L and 50 μg/L, or CYN/MC mixture at 5 + 5 μg/L and 25 + 25 μg/L. Extracts from M. aeruginosa and C. ovalisporum were used as sources of toxins. The study revealed growth inhibition of the aerial part (Leaves) in both species when treated with 50µg/L of MC, CYN and CYN/MC mixture. MC showed to be more harmful to plant growth than CYN. Moreover spinach leaves growth was inhibited by both 5 + 5 and 25 + 25 µg/L CYN/MC mixtures, whereas lettuce leaves growth was inhibited only by 25 + 25 µg/L CYN/MC mixture. Overall, growth data evidence increased sensitivity of spinach to cyanotoxins in comparison to lettuce. On the other hand, plants exposed to CYN/MC mixture showed differential accumulation of CYN and MC. In addition, CYN, but not MC, was translocated from the roots to the leaves. CYN and MC affected the levels of minerals particularly in plant roots. The elements most affected were Ca, K and Mg. However, in leaves K was the mineral that was affected by exposure to cyanotoxins.

ACS Style

Maria Llana-Ruiz-Cabello; Angeles Jos; Ana Cameán; Flavio Oliveira; Aldo Barreiro; Joana Machado; Joana Azevedo; Edgar Pinto; Agostinho Almeida; Alexandre Campos; Vitor Vasconcelos; Marisa Freitas. Analysis of the Use of Cylindrospermopsin and/or Microcystin-Contaminated Water in the Growth, Mineral Content, and Contamination of Spinacia oleracea and Lactuca sativa. Toxins 2019, 11, 624 .

AMA Style

Maria Llana-Ruiz-Cabello, Angeles Jos, Ana Cameán, Flavio Oliveira, Aldo Barreiro, Joana Machado, Joana Azevedo, Edgar Pinto, Agostinho Almeida, Alexandre Campos, Vitor Vasconcelos, Marisa Freitas. Analysis of the Use of Cylindrospermopsin and/or Microcystin-Contaminated Water in the Growth, Mineral Content, and Contamination of Spinacia oleracea and Lactuca sativa. Toxins. 2019; 11 (11):624.

Chicago/Turabian Style

Maria Llana-Ruiz-Cabello; Angeles Jos; Ana Cameán; Flavio Oliveira; Aldo Barreiro; Joana Machado; Joana Azevedo; Edgar Pinto; Agostinho Almeida; Alexandre Campos; Vitor Vasconcelos; Marisa Freitas. 2019. "Analysis of the Use of Cylindrospermopsin and/or Microcystin-Contaminated Water in the Growth, Mineral Content, and Contamination of Spinacia oleracea and Lactuca sativa." Toxins 11, no. 11: 624.

Journal article
Published: 29 May 2019 in Toxins
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Tetrodotoxin (TTX) is a potent alkaloid typically from tropical ecosystems, but in the last decade its presence has been more pronounced in the temperate waters of the Atlantic. In its last scientific opinion, the European Food Safety Authority (EFSA) stressed the need for data regarding TTX prevalence in European waters. To address EFSA’s concerns, benthic organisms such as mollusks, crustaceans, echinoderms and fish with different feeding habits were collected along the Portuguese continental coast, islands (São Miguel, Azores, and Madeira) and the northwestern Moroccan coast. A total of 165 samples were analyzed by ultra high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) and ultra high performance chromatography mass spectrometry (UHPLC-MS/MS). Geographical tendencies were detected as follows, by descending order: S. Miguel Island (Azores), Moroccan coast, Madeira Island and Portuguese continental coast. The toxin amounts detected were significant, above the Dutch limit value established in 2017, showing the importance and the need for continuity of these studies to gain more knowledge about the prevalence of these toxins, unraveling new vectors, in order to better assess human health risk. This work represents a general overview of new TTX bearers (7) most of them in gastropods (Patella depressa, Nucella lapillus, Onchidella celtica and Aplysia depilans), followed by echinoderms (Echinus esculentus and Ophidiaster ophidianus) and puffer fish Sphoeroides marmoratus.

ACS Style

Marisa Silva; Inés Rodríguez; Aldo Barreiro; Manfred Kaufmann; Ana Isabel Neto; Meryem Hassouani; Brahim Sabour; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. Tetrodotoxins Occurrence in Non-Traditional Vectors of the North Atlantic Waters (Portuguese Maritime Territory, and Morocco Coast). Toxins 2019, 11, 306 .

AMA Style

Marisa Silva, Inés Rodríguez, Aldo Barreiro, Manfred Kaufmann, Ana Isabel Neto, Meryem Hassouani, Brahim Sabour, Amparo Alfonso, Luis M. Botana, Vitor Vasconcelos. Tetrodotoxins Occurrence in Non-Traditional Vectors of the North Atlantic Waters (Portuguese Maritime Territory, and Morocco Coast). Toxins. 2019; 11 (6):306.

Chicago/Turabian Style

Marisa Silva; Inés Rodríguez; Aldo Barreiro; Manfred Kaufmann; Ana Isabel Neto; Meryem Hassouani; Brahim Sabour; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. 2019. "Tetrodotoxins Occurrence in Non-Traditional Vectors of the North Atlantic Waters (Portuguese Maritime Territory, and Morocco Coast)." Toxins 11, no. 6: 306.

Primary research paper
Published: 13 March 2019 in Hydrobiologia
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Picocyanobacteria represent the main fraction of ocean primary production and, due to the effects of ocean acidification and an increase in seawater temperature, this group is expected to be favored in future scenarios. For this reason, we studied the ecophysiological response of picocyanobacteria to increases in water temperature by testing the allelopathic activity of a subpolar strain of Synechocystis sp., grown at a temperature range of 10–20°C, against coexisting microalgae. We showed that cell-free filtrates of Synechocystis sp. inhibited growth and the maximum quantum yields of PSII (Fv/Fm) of Porphyridium purpureum, Fistulifera sp., and Chlorella vulgaris, and the negative effect of the picocyanobacterium was stronger at the highest temperatures at which Synechocystis sp. was grown. Similarly, in P. purpureum, the effect of filtrates reduced chlorophyll a (Chl a) and carotenoids (Car) content, and the effect depended on the temperature at which the picocyanobacterium was grown. The lipid content was observed to increase in all species, and the allelopathic effect was the strongest at the highest temperature in which Synechocystis sp. was grown. The results of the present study predict a stronger allelopathic effect of picocyanobacteria against competitors with rising temperatures. They also suggest that the potential effect of climate change would benefit this group in future phytoplankton communities.

ACS Style

Aldo Barreiro Felpeto; Sylwia Śliwińska-Wilczewska; Marek Klin; Zofia Konarzewska; Vitor Vasconcelos. Temperature-dependent impacts of allelopathy on growth, pigment, and lipid content between a subpolar strain of Synechocystis sp. CCBA MA-01 and coexisting microalgae. Hydrobiologia 2019, 835, 117 -128.

AMA Style

Aldo Barreiro Felpeto, Sylwia Śliwińska-Wilczewska, Marek Klin, Zofia Konarzewska, Vitor Vasconcelos. Temperature-dependent impacts of allelopathy on growth, pigment, and lipid content between a subpolar strain of Synechocystis sp. CCBA MA-01 and coexisting microalgae. Hydrobiologia. 2019; 835 (1):117-128.

Chicago/Turabian Style

Aldo Barreiro Felpeto; Sylwia Śliwińska-Wilczewska; Marek Klin; Zofia Konarzewska; Vitor Vasconcelos. 2019. "Temperature-dependent impacts of allelopathy on growth, pigment, and lipid content between a subpolar strain of Synechocystis sp. CCBA MA-01 and coexisting microalgae." Hydrobiologia 835, no. 1: 117-128.

Journal article
Published: 01 March 2018 in Journal of Plankton Research
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Allelopathic compounds produced by cyanobacteria may play important roles in the dynamics of several biological systems. The main goal of this work was to investigate reciprocal allelopathic effects between species of two relevant groups of marine cyanobacteria: the picocyanobacterium Synechococcus sp. and the filamentous species Nodularia spumigena. Our experimental design consisted of cell-free filtrates and co-cultures. We demonstrated that Synechococcus sp. had a strong inhibitory effect on N. spumigena, and surprisingly, there was no reciprocal effect from the filamentous cyanobacteria. We detected this effect both in co-cultures and cell-free filtrate bioassays. These effects depended on light conditions in the culture of the allelopathic species. This allelopathic effect against N. spumigena triggered physiological responses leading to reduced chlorophyll a (Chl a) and carotenoid (Car) content, cell shape distortions and, often, cell lysis. Surprisingly, no evidence was found of allelopathic effects of our strain of N. spumigena (a well-known allelopathic species) against Synechococcus sp. These results support the fact that allelopathic interactions between Synechococcus sp. and N. spumigena may be a factor influencing the formation of massive bloom of the former organisms in many aquatic ecosystems, like the Baltic Sea, where the two species constitute a relevant fraction of phytoplankton biomass.

ACS Style

Aldo Barreiro Felpeto; Sylwia Śliwińska-Wilczewska; Ilona Złoch; Vitor Vasconcelos. Light-dependent cytolysis in the allelopathic interaction between picoplanktic and filamentous cyanobacteria. Journal of Plankton Research 2018, 40, 165 -177.

AMA Style

Aldo Barreiro Felpeto, Sylwia Śliwińska-Wilczewska, Ilona Złoch, Vitor Vasconcelos. Light-dependent cytolysis in the allelopathic interaction between picoplanktic and filamentous cyanobacteria. Journal of Plankton Research. 2018; 40 (2):165-177.

Chicago/Turabian Style

Aldo Barreiro Felpeto; Sylwia Śliwińska-Wilczewska; Ilona Złoch; Vitor Vasconcelos. 2018. "Light-dependent cytolysis in the allelopathic interaction between picoplanktic and filamentous cyanobacteria." Journal of Plankton Research 40, no. 2: 165-177.

Review
Published: 20 January 2018 in Toxins
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Picocyanobacteria are extremely important organisms in the world’s oceans and freshwater ecosystems. They play an essential role in primary production and their domination in phytoplankton biomass is common in both oligotrophic and eutrophic waters. Their role is expected to become even more relevant with the effect of climate change. However, this group of photoautotrophic organisms still remains insufficiently recognized. Only a few works have focused in detail on the occurrence of massive blooms of picocyanobacteria, their toxicity and allelopathic activity. Filling the gap in our knowledge about the mechanisms involved in the proliferation of these organisms could provide a better understanding of aquatic environments. In this review, we gathered and described recent information about allelopathic activity of picocyanobacteria and occurrence of their massive blooms in many aquatic ecosystems. We also examined the relationships between climate change and representative picocyanobacterial genera from freshwater, brackish and marine ecosystems. This work emphasizes the importance of studying the smallest picoplanktonic fractions of cyanobacteria.

ACS Style

Sylwia Śliwińska-Wilczewska; Jakub Maculewicz; Aldo Barreiro Felpeto; Adam Latała. Allelopathic and Bloom-Forming Picocyanobacteria in a Changing World. Toxins 2018, 10, 48 .

AMA Style

Sylwia Śliwińska-Wilczewska, Jakub Maculewicz, Aldo Barreiro Felpeto, Adam Latała. Allelopathic and Bloom-Forming Picocyanobacteria in a Changing World. Toxins. 2018; 10 (1):48.

Chicago/Turabian Style

Sylwia Śliwińska-Wilczewska; Jakub Maculewicz; Aldo Barreiro Felpeto; Adam Latała. 2018. "Allelopathic and Bloom-Forming Picocyanobacteria in a Changing World." Toxins 10, no. 1: 48.

Research article
Published: 01 January 2018 in Marine and Freshwater Research
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The production and release of allelopathic compounds is an important adaptation by which some species of cyanobacteria can achieve a competitive advantage over other primary producers. In the present study we tested the allelopathic activity of the picocyanobacterium Synechococcus sp. against the following coexisting unicellular eukaryote microalgae: Porphyridium purpureum, Stichococcus bacillaris, Prymnesium parvum and Nitzschia dissipata. With these species, we covered a wide range of taxonomic groups. We demonstrated that both the addition of Synechococcus sp. cell-free filtrate and coculture inhibited the growth, chlorophyll content and photosynthetic rate of P. purpureum and S. bacillaris. Conversely, P. parvum, a well-known mixotroph, was positively affected by both Synechococcus sp. treatments. In contrast, N. dissipata was not affected by either the picocyanobacterial filtrate or coculture. These results suggest that the negative allelopathic effect is related to a reduction in the photosynthetic rate, and that Synechococcus sp. allelopathy should be taken into account in the interactions between picocyanobacteria and eukaryote competitors coexisting in a planktonic system.

ACS Style

Sylwia Śliwińska-Wilczewska; Aldo Barreiro Felpeto; Jakub Maculewicz; Amanda Sobczyk; Vitor Vasconcelos; Adam Latała. Allelopathic activity of the picocyanobacterium Synechococcus sp. on unicellular eukaryote planktonic microalgae. Marine and Freshwater Research 2018, 69, 1472 -1479.

AMA Style

Sylwia Śliwińska-Wilczewska, Aldo Barreiro Felpeto, Jakub Maculewicz, Amanda Sobczyk, Vitor Vasconcelos, Adam Latała. Allelopathic activity of the picocyanobacterium Synechococcus sp. on unicellular eukaryote planktonic microalgae. Marine and Freshwater Research. 2018; 69 (9):1472-1479.

Chicago/Turabian Style

Sylwia Śliwińska-Wilczewska; Aldo Barreiro Felpeto; Jakub Maculewicz; Amanda Sobczyk; Vitor Vasconcelos; Adam Latała. 2018. "Allelopathic activity of the picocyanobacterium Synechococcus sp. on unicellular eukaryote planktonic microalgae." Marine and Freshwater Research 69, no. 9: 1472-1479.

Journal article
Published: 01 November 2017 in Journal of Experimental Marine Biology and Ecology
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Allelopathy of picocyanobacterium Synechococcus sp. may be involved in the formation of massive blooms and their subsequent global expansion in many aquatic ecosystems. However, the effect of the allelopathic activity of this species on filamentous cyanobacteria remains unknown. In this study, we tested the allelopathic activity of Synechococcus sp. on growth, pigment content and chlorophyll fluorescence of filamentous cyanobacteria Aphanizomenon flos-aquae, Nostoc sp., Phormidium sp. and Rivularia sp. by single and repeated addition of cell-free filtrate. Negative effects against Nostoc sp. and Phormidium sp. were amplified by repeated filtrate additions compared with single filtrate addition. A maximum reduction in growth and a maximum quantum yield of photosystem II (PSII) photochemistry (Fv/Fm) relative to controls were observed for Phormidium sp. However, the addition of picocyanobacterial filtrate stimulated the growth and Fv/Fm of A. flos-aquae. Synechococcus sp. filtrate had no allelopathic effects on the growth and Fv/Fm of Rivularia sp. Moreover, filtrates caused significant decreases in the chlorophyll a (Chl a) contents of Phormidium sp. and Rivularia sp. cells. The addition of filtrate also resulted in increased cell carotenoid (Car) content in A. flos-aquae and Nostoc sp. These results showed for the first time that picocyanobacterium Synechococcus sp. negatively and positively affected coexisting filamentous cyanobacteria.

ACS Style

Sylwia Śliwińska-Wilczewska; Jakub Maculewicz; Aldo Barreiro Felpeto; Vitor Vasconcelos; Adam Latała. Allelopathic activity of picocyanobacterium Synechococcus sp. on filamentous cyanobacteria. Journal of Experimental Marine Biology and Ecology 2017, 496, 16 -21.

AMA Style

Sylwia Śliwińska-Wilczewska, Jakub Maculewicz, Aldo Barreiro Felpeto, Vitor Vasconcelos, Adam Latała. Allelopathic activity of picocyanobacterium Synechococcus sp. on filamentous cyanobacteria. Journal of Experimental Marine Biology and Ecology. 2017; 496 ():16-21.

Chicago/Turabian Style

Sylwia Śliwińska-Wilczewska; Jakub Maculewicz; Aldo Barreiro Felpeto; Vitor Vasconcelos; Adam Latała. 2017. "Allelopathic activity of picocyanobacterium Synechococcus sp. on filamentous cyanobacteria." Journal of Experimental Marine Biology and Ecology 496, no. : 16-21.

Journal article
Published: 19 July 2017 in Oikos
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It has been hypothesized that allelopathy can prevent competitive exclusion and promote phytoplankton diversity in aquatic ecosystems, where numerous species coexist on a limited number of resources. However, experimental proof-of-principle is not available to support this hypothesis. Here we present the first experimental evidence to support this hypothesis by demonstrating that allelopathy promotes the coexistence of two phytoplankton species, Ankistrodesmus falcatus and Oscillatoria sp., that compete for a single limiting nutrient. By performing long-term competition experiments in nitrate-limited continuous cultures, and by describing the population dynamics using a mechanistic model, we demonstrate that when allelopathy comes into play, one of the following outcomes is possible depending on the relative initial abundances of the species: dominance of the stronger competitor for nitrate (the non-allelopathic species), oscillatory coexistence, or dominance of the weaker competitor (the allelopathic species). Our model analysis revealed that sustained oscillatory coexistence of the two species would be a common outcome of this experiment. Our study confirms for the first time, based on laboratory experiments combined with mechanistic models, that allelopathy can alter the predicted outcome of inter-specific competition in a nutrient-limited environment and increase the potential for the coexistence of more species than resources, thereby contributing to the identification of endogenous mechanisms that explain the extreme diversity of phytoplankton communities.

ACS Style

Aldo Barreiro Felpeto; Shovonlal Roy; Vitor Vasconcelos. Allelopathy prevents competitive exclusion and promotes phytoplankton biodiversity. Oikos 2017, 127, 85 -98.

AMA Style

Aldo Barreiro Felpeto, Shovonlal Roy, Vitor Vasconcelos. Allelopathy prevents competitive exclusion and promotes phytoplankton biodiversity. Oikos. 2017; 127 (1):85-98.

Chicago/Turabian Style

Aldo Barreiro Felpeto; Shovonlal Roy; Vitor Vasconcelos. 2017. "Allelopathy prevents competitive exclusion and promotes phytoplankton biodiversity." Oikos 127, no. 1: 85-98.

Journal article
Published: 08 December 2015 in Toxins
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Okadaic acid and its analogues are potent phosphatase inhibitors that cause Diarrheic Shellfish Poisoning (DSP) through the ingestion of contaminated shellfish by humans. This group of toxins is transmitted worldwide but the number of poisoning incidents has declined over the last 20 years due to legislation and monitoring programs that were implemented for bivalves. In the summer of 2012 and 2013, we collected a total of 101 samples of 22 different species that were made up of benthic and subtidal organisms such echinoderms, crustaceans, bivalves and gastropods from Madeira, São Miguel Island (Azores archipelago) and the northwestern coast of Morocco. The samples were analyzed by UPLC-MS/MS. Our main objective was to detect new vectors for these biotoxins. We can report nine new vectors for these toxins in the North Atlantic: Astropecten aranciacus, Arbacia lixula, Echinaster sepositus, Holothuria sanctori, Ophidiaster ophidianus, Onchidella celtica, Aplysia depilans, Patella spp., and Stramonita haemostoma. Differences in toxin contents among the species were found. Even though low concentrations were detected, the levels of toxins that were present, especially in edible species, indicate the importance of these types of studies. Routine monitoring should be extended to comprise a wider number of vectors other than for bivalves of okadaic acid and its analogues.

ACS Style

Marisa Silva; Inés Rodriguez; Aldo Barreiro; Manfred Kaufmann; Ana Isabel Neto; Meryem Hassouani; Brahim Sabour; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. New Invertebrate Vectors of Okadaic Acid from the North Atlantic Waters—Portugal (Azores and Madeira) and Morocco. Toxins 2015, 7, 5337 -5347.

AMA Style

Marisa Silva, Inés Rodriguez, Aldo Barreiro, Manfred Kaufmann, Ana Isabel Neto, Meryem Hassouani, Brahim Sabour, Amparo Alfonso, Luis M. Botana, Vitor Vasconcelos. New Invertebrate Vectors of Okadaic Acid from the North Atlantic Waters—Portugal (Azores and Madeira) and Morocco. Toxins. 2015; 7 (12):5337-5347.

Chicago/Turabian Style

Marisa Silva; Inés Rodriguez; Aldo Barreiro; Manfred Kaufmann; Ana Isabel Neto; Meryem Hassouani; Brahim Sabour; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. 2015. "New Invertebrate Vectors of Okadaic Acid from the North Atlantic Waters—Portugal (Azores and Madeira) and Morocco." Toxins 7, no. 12: 5337-5347.

Journal article
Published: 21 September 2015 in Toxins
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Ciguatera fish poisoning (CFP) is a syndrome caused by the ingestion of fish contaminated with Ciguatoxins (CTXs). These phycotoxins are produced mainly by dinoflagellates that belong to the genus Gambierdiscus that are transformed in more toxic forms in predatory fish guts, and are more present in the Indo-Pacific and Caribbean areas. It is estimated that CFP causes per year more than 10,000 intoxications worldwide. With the rise of water temperature and anthropogenic intervention, it is important to study the prevalence of CFP in more temperate waters. Through inter- and subtidal sampling, 22 species of organisms were collected, in Madeira and Azores archipelagos and in the northwestern Moroccan coast, during September of 2012 and June and July of 2013. A total of 94 samples of 22 different species of bivalves, gastropods, echinoderms and crustaceans where analyzed by Ultra Performance Liquid Chromatography-Mass Spectometry-Ion Trap-Time of Flight (UPLC-MS-IT-TOF) and Ultra Performance Chromatography- Mass Spectrometry (UPLC-MS). Our main aim was to detect new vectors and ascertain if there were some geographical differences. We detected for the first time putative CTXs in echinoderms, in two starfish species—M. glacialis and O. ophidianus. We detected differences regarding uptake values by organisms and geographical location. Toxin amounts were significant, showing the importance and the need for continuity of these studies to gain more knowledge about the prevalence of these toxins, in order to better access human health risk. In addition, we suggest monitoring of these toxins should be extended to other vectors, starfish being a good alternative for protecting and accessing human health risk.

ACS Style

Marisa Silva; Inés Rodriguez; Aldo Barreiro; Manfred Kaufmann; Ana Isabel Neto; Meryem Hassouani; Brahim Sabour; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. First Report of Ciguatoxins in Two Starfish Species: Ophidiaster ophidianus and Marthasterias glacialis. Toxins 2015, 7, 3740 -3757.

AMA Style

Marisa Silva, Inés Rodriguez, Aldo Barreiro, Manfred Kaufmann, Ana Isabel Neto, Meryem Hassouani, Brahim Sabour, Amparo Alfonso, Luis M. Botana, Vitor Vasconcelos. First Report of Ciguatoxins in Two Starfish Species: Ophidiaster ophidianus and Marthasterias glacialis. Toxins. 2015; 7 (9):3740-3757.

Chicago/Turabian Style

Marisa Silva; Inés Rodriguez; Aldo Barreiro; Manfred Kaufmann; Ana Isabel Neto; Meryem Hassouani; Brahim Sabour; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. 2015. "First Report of Ciguatoxins in Two Starfish Species: Ophidiaster ophidianus and Marthasterias glacialis." Toxins 7, no. 9: 3740-3757.

Journal article
Published: 14 March 2014 in Aquatic Ecology
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We chose four species of freshwater phytoplankton: the chlorophyceans Ankistrodesmus falcatus, Chlamydomonas reinhardtii and Selenastrum capricornutum, and the cyanobacteria Oscillatoria sp. in order to study their competitive abilities for nitrate and their allelopathic properties. We parameterized models of nitrate uptake and growth with laboratory experiments. According to them, the species were ranked (from the best to the worst competitors): S. capricornutum, C. reinhardtii, A. falcatus and Oscillatoria sp. C. reinhardtii and Oscillatoria sp. were previously reported as allelopathic. In the present work, Oscillatoria sp. was allelopathic only against A. falcatus. However, none of our species was sensitive to C. reinhardtii. Additionally, we found an unknown allelopathic effect of A. falcatus against Oscillatoria sp. Our findings point out the high specificity of allelopathic interactions. With these data, we constructed a model of interspecific competition for nitrate, including allelopathic interactions. By performing model simulations, we studied how three factors influence the outcome of competition: relative abundance of competing species, resistance to allelopathy, and nitrate concentration. Our simulations showed that the initial ratio of species abundances will significantly determine the outcome of competition. If the worst competitor was the allelopathic species, the more it needs to outnumber the competing species, unless it is very sensitive to allelopathy (not defended). Nitrate has an important influence, showing a non-intuitive outcome of competition experiments at low nitrate concentrations, where the worst competitor (allelopathic species) wins competition in the majority of cases, whereas at intermediate concentrations, the better competitor dominates except for unfavorable ratios of abundances. With the increased amounts of nitrate, conditions again favor the worst competitor (the stronger allelopathic species). Despite the potential for two species coexistence showed by previous theoretical analysis of systems was similar to ours, our simulations did not detect this outcome. We hypothesized that this is due to the strong allelopathic effect of Oscillatoria sp.

ACS Style

Aldo Barreiro; Vitor Vasconcelos. Interactions between allelopathic properties and growth kynetics in four freshwater phytoplankton species studied by model simulations. Aquatic Ecology 2014, 48, 191 -205.

AMA Style

Aldo Barreiro, Vitor Vasconcelos. Interactions between allelopathic properties and growth kynetics in four freshwater phytoplankton species studied by model simulations. Aquatic Ecology. 2014; 48 (2):191-205.

Chicago/Turabian Style

Aldo Barreiro; Vitor Vasconcelos. 2014. "Interactions between allelopathic properties and growth kynetics in four freshwater phytoplankton species studied by model simulations." Aquatic Ecology 48, no. 2: 191-205.

Journal article
Published: 31 December 2013 in Marine Drugs
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The oceans remain a major source of natural compounds with potential in pharmacology. In particular, during the last few decades, marine cyanobacteria have been in focus as producers of interesting bioactive compounds, especially for the treatment of cancer. In this study, the anticancer potential of extracts from twenty eight marine cyanobacteria strains, belonging to the underexplored picoplanktonic genera, Cyanobium, Synechocystis and Synechococcus, and the filamentous genera, Nodosilinea, Leptolyngbya, Pseudanabaena and Romeria, were assessed in eight human tumor cell lines. First, a crude extract was obtained by dichloromethane:methanol extraction, and from it, three fractions were separated in a Si column chromatography. The crude extract and fractions were tested in eight human cancer cell lines for cell viability/toxicity, accessed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and lactic dehydrogenase release (LDH) assays. Eight point nine percent of the strains revealed strong cytotoxicity; 17.8% showed moderate cytotoxicity, and 14.3% assays showed low toxicity. The results obtained revealed that the studied genera of marine cyanobacteria are a promising source of novel compounds with potential anticancer activity and highlight the interest in also exploring the smaller filamentous and picoplanktonic genera of cyanobacteria.

ACS Style

Margarida Costa; Mónica Garcia; João Costa-Rodrigues; Maria Sofia Costa; Maria João Ribeiro; Maria Helena Fernandes; Piedade Barros; Aldo Barreiro; Vitor Vasconcelos; Rosário Martins. Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds. Marine Drugs 2013, 12, 98 -114.

AMA Style

Margarida Costa, Mónica Garcia, João Costa-Rodrigues, Maria Sofia Costa, Maria João Ribeiro, Maria Helena Fernandes, Piedade Barros, Aldo Barreiro, Vitor Vasconcelos, Rosário Martins. Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds. Marine Drugs. 2013; 12 (1):98-114.

Chicago/Turabian Style

Margarida Costa; Mónica Garcia; João Costa-Rodrigues; Maria Sofia Costa; Maria João Ribeiro; Maria Helena Fernandes; Piedade Barros; Aldo Barreiro; Vitor Vasconcelos; Rosário Martins. 2013. "Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds." Marine Drugs 12, no. 1: 98-114.

Journal article
Published: 21 August 2013 in Journal Of Plankton Research
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Because we found previously that Chlamydomonas reinhardtii produces allelochemicals to which the rotifer Brachionus calyciflorus is sensitive, we explored its effects on other freshwater plankton. We used Chlamydomonas under light-, nitrogen- and phosphorus-limitation to test its allelopathic effect on Microcystis aeruginosa, Cryptomonas ozolinii, Ochromonas danica, Tetrahymena thermophila and Paramecium aurelia. Allelopathy depended strongly on the target organism. Only Cryptomonas suffered a marked negative effect. Among the resource limiting regimes, light limitation exerted the greatest effect.

ACS Style

Aldo Barreiro; Nelson G. Hairston; Aldo Barreiro Felpeto. The influence of resource limitation on the allelopathic effect of Chlamydomonas reinhardtii on other unicellular freshwater planktonic organisms. Journal Of Plankton Research 2013, 35, 1339 -1344.

AMA Style

Aldo Barreiro, Nelson G. Hairston, Aldo Barreiro Felpeto. The influence of resource limitation on the allelopathic effect of Chlamydomonas reinhardtii on other unicellular freshwater planktonic organisms. Journal Of Plankton Research. 2013; 35 (6):1339-1344.

Chicago/Turabian Style

Aldo Barreiro; Nelson G. Hairston; Aldo Barreiro Felpeto. 2013. "The influence of resource limitation on the allelopathic effect of Chlamydomonas reinhardtii on other unicellular freshwater planktonic organisms." Journal Of Plankton Research 35, no. 6: 1339-1344.

Journal article
Published: 05 June 2013 in Marine Drugs
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The prevalence of poisoning events due to harmful algal blooms (HABs) has declined during the last two decades through monitoring programs and legislation, implemented mainly for bivalves. However, new toxin vectors and emergent toxins pose a challenge to public health. Several locations on the Portuguese coast were surveyed between 2009 and 2010 for three distinct biotoxin groups [saxitoxin (PST), spirolide (SPX) and okadaic acid (OA)], in 14 benthic species of mollusks and echinoderms. Our main goals were to detect new vectors and unravel the seasonal and geographical patterns of these toxins. PSTs were analyzed by the Lawrence method, SPXs by LC-MS/MS, and OA by LC-MS/MS and UPLC-MS/MS. We report 16 new vectors for these toxins in the North Atlantic. There were differences in toxin contents among species, but no significant geographical or seasonal patterns were found. Our results suggest that legislation should be adjusted to extend the monitoring of marine toxins to a wider range of species besides edible bivalves.

ACS Style

Marisa Silva; Aldo Barreiro; Paula Rodriguez; Paz Otero; Joana Azevedo; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. New Invertebrate Vectors for PST, Spirolides and Okadaic Acid in the North Atlantic. Marine Drugs 2013, 11, 1936 -1960.

AMA Style

Marisa Silva, Aldo Barreiro, Paula Rodriguez, Paz Otero, Joana Azevedo, Amparo Alfonso, Luis M. Botana, Vitor Vasconcelos. New Invertebrate Vectors for PST, Spirolides and Okadaic Acid in the North Atlantic. Marine Drugs. 2013; 11 (6):1936-1960.

Chicago/Turabian Style

Marisa Silva; Aldo Barreiro; Paula Rodriguez; Paz Otero; Joana Azevedo; Amparo Alfonso; Luis M. Botana; Vitor Vasconcelos. 2013. "New Invertebrate Vectors for PST, Spirolides and Okadaic Acid in the North Atlantic." Marine Drugs 11, no. 6: 1936-1960.

Journal article
Published: 03 May 2013 in Limnology and Oceanography
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The nature of the growth-rate response of grazing zooplankton to algal concentration depends critically upon food quality, which is often a plastic function of environmental conditions. Phytoplankton quality depends upon nutrient and light availability, which together affect cell C : P and C : N, so that the links from external environment to phytoplankton and then to zooplankton can vary in ways that influence consumer-resource interaction strengths, and ultimately, food web structure. We measured the effects of resource-limitation on the population growth rate of rotifers, Brachionus calyciflorus, feeding on the alga Chlamydomonas reinhardtii, and grown in nitrogen-limiting, phosphorus-limiting, or nutrient-sufficient conditions. Consumption of nutrient-sufficient algae produced a Type II numerical response with a low half saturation constant (Ks) and high asymptotic growth rate (λmax) consistent with high food quality; P-limited algae also yielded a Type II response but with high Ks and low λmax, meaning poor food quality. In contrast, N-limited Chlamydomonas resulted in a novel Type IV numerical response in which rotifer growth rate rose to an intermediate λmax and then declined at the highest algal densities, suggesting the production of toxic compounds whose negative effect on growth increases as a function of cell density. Experiments with mixed diets confirmed that N-limited food was indeed toxic, whereas P-limited food was simply nutritionally deficient compared with light-limited algae. Additional experiments showed that even the filtrate of N-limited algae can be detrimental to rotifer growth.

ACS Style

Aldo Barreiro Felpeto; Nelson G. Hairston Jr.. Indirect bottom-up control of consumer-resource dynamics: Resource-driven algal quality alters grazer numerical response. Limnology and Oceanography 2013, 58, 827 -838.

AMA Style

Aldo Barreiro Felpeto, Nelson G. Hairston Jr.. Indirect bottom-up control of consumer-resource dynamics: Resource-driven algal quality alters grazer numerical response. Limnology and Oceanography. 2013; 58 (3):827-838.

Chicago/Turabian Style

Aldo Barreiro Felpeto; Nelson G. Hairston Jr.. 2013. "Indirect bottom-up control of consumer-resource dynamics: Resource-driven algal quality alters grazer numerical response." Limnology and Oceanography 58, no. 3: 827-838.