Spirulina, a cyanobacterial biomass, is renowned for its high protein content and bioactive compounds, making it a promising candidate for health-promoting applications. This study explores the ultrasound-assisted deep eutectic solvent (DES) extraction technique for isolating antioxidants and anticancer proteins from Spirulina biomass, aiming to enhance extraction efficiency and preserve protein bioactivity. The extraction process was optimized using response surface methodology (RSM), varying parameters such as biomass concentration, sonication amplitude, and extraction duration. The optimized extraction conditions—5% biomass concentration, 40% sonication amplitude, and 22-minute extraction—achieved a high protein yield of 80.62%, with a protein concentration of 442.88 mg/g extract and an essential amino acid content of 39.91%. The extracted proteins exhibited remarkable bioactivity, including strong antioxidant properties, with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of 0.25 mg GAE/g, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity of 0.58 mg TE/g, and ferric reducing antioxidant power (PFRAP) of 9.63 mg gallic acid equivalent (GAE)/g. Additionally, the protein extract displayed selective cytotoxicity against colorectal cancer cell lines, with half-maximal inhibitory concentration (IC₅₀) values of 10.25 mg/mL for Caco-2 and 15.40 mg/mL for HT-29 cells, while maintaining low toxicity towards normal Vero cells. Apoptosis rates of 70.43% in Caco-2 and 51.33% in HT-29 cells further confirm the anticancer potential of the extract. The functional properties of the extracted protein, including high foaming capacity (100%), emulsifying capacity (94.05%), and digestibility (85.77%), underscore its potential for diverse applications in food, pharmaceutical, and nutraceutical industries. This eco-friendly and efficient extraction approach aligns with sustainable development goals and offers a viable strategy for harnessing Spirulina’s bioactive potential.

Edible insect honeybee brood and natural honey are rich in proteins and saccharides, with inherent bioactive properties such as antioxidant activity. To enhance their antioxidative potential under simple thermal conditions, this research employed spontaneous aging via a moist-dried heating process, primarily driven by the Maillard reaction. Honeybee brood bio-peptides (HBb-BPs), produced through Rhizopus oligosporus fermentation, were mixed with honey in varying ratios of 70:30, 50:50, and 30:70 (%w/w). The mixtures underwent interaction under controlled conditions (60 °C for 20 days at ~75% relative humidity). A comparative analysis was performed on the mixtures before and after the thermal interaction, focusing on chemical characterization and antioxidant activity (ABTS, DPPH, and FRAP assays). Results revealed that the post-process mixtures exhibited significantly enhanced antioxidant activity, with higher honey concentrations correlating to greater antioxidative effects. Furthermore, allulose and mannose were detected after processing, while levels of fructose, glucose, and free amino acids decreased. These changes likely indicate the formation of complex compounds, molecular rearrangements, and the production of phenolic compounds that contributed to the increased antioxidative capacity. This study highlights the pivotal role of the Maillard reaction in augmenting antioxidant activity, elucidates changes in sugar–amino acid interactions, and validates the effectiveness of the moist-dried heating process. These findings provide valuable insights for potential future applications of this simple and scalable method.

This study addresses the growing interest in natural functional ingredients by evaluating the prebiotic and health-promoting functions of honeybee brood biopeptides (HBb-Bps) and their conjugates. The purpose was to investigate their antioxidant activities, enzyme inhibition properties, and effects on probiotic growth and short-chain fatty acid (SCFA) production. The HBb-Bps were conjugated with honey, glucose, and fructose via the Maillard reaction. Antioxidant activities were assessed using DPPH and ABTS assays. The inhibitory effects on amylase, pancreatic lipase, and the angiotensin-converting enzyme (ACE) were measured. Probiotic growth and SCFA production were evaluated using L. plantarum TISTR846, and L. lactis TISTR1464. The HBb-Bps and their conjugates exhibited enhanced antioxidant activities post-Maillard reaction. They showed moderate enzyme inhibition, which decreased after conjugation. However, ACE inhibition increased with conjugation. The HBb-Bps significantly promoted probiotic growth and SCFA production, with further enhancement by the Maillard reaction. Overall, the HBb-Bps and their conjugates demonstrate significant prebiotic and health-promoting functions, suggesting their potential as natural ingredients in functional foods and nutraceuticals. Further research should focus on the in vivo effects and, given their solubility and stability these biopeptides could be incorporated into functional food formulations, such as health beverages, protein bars, and other fortified foods designed to deliver specific health benefits.

This study investigates the development of protein powder from honey bee drone broods using foam-mat drying, a scalable method suitable for community enterprises, as well as the preservation of bee broods as a food ingredient. Initially, honey bee broods were pre-treated by boiling and steaming, with steamed bee brood (S_BB) showing the highest protein content (44.71 g/100 g dry basis). A factorial design optimized the powder formulation through the foam-mat drying process, incorporating varying concentrations of S_BB, glycerol monostearate (GMS), and carboxymethyl cellulose (CMC). The physicochemical properties of the resulting powder, including yield, color spaces, water activity, solubility, protein content, and total amino acids, were evaluated. The results showed that foam-mat drying produced a stable protein powder. The binders (CMC and GMS) increased the powder’s yield and lightness but negatively affected the hue angle (yellow-brown), protein content, and amino acid content. The optimal quantities of the three variables (S_BB, GMS, and CMC) were determined to be 30 g, 6 g, and 1.5 g, or 80%, 16%, and 4%, respectively. Under this formulation, the protein powder exhibited a protein content of 19.89 g/100 g. This research highlights the potential of bee brood protein powder as a sustainable and nutritious alternative protein source, enhancing food diversification and security.

Honeybee brood biopeptides (HBb-Bps) are a novel source of bioactive compounds with potential health benefits. In this study, HBb-Bps were conjugated with honey via a Maillard reaction and their physicochemical properties, digestive stability, antioxidant capacity, and anti-obesogenic effects were evaluated. Simulated gastrointestinal digestion revealed significantly enhanced resistance after conjugation, with the residual content increasing from 46.99% for native HBb-Bps to 86.12% for the honey-conjugated forms; furthermore, antioxidant activity was largely preserved according to the DPPH and ABTS assays. In the in vivo experiments, 30 male BrlHan: WIST@Jcl (GALAS) (Wistar) rats were fed a high-fat diet (HFD) to induce obesity and orally administered honey-conjugated HBb-Bps at doses of 200, 500, or 1000 mg/kg body weight for 16 weeks. The highest dose led to significant reductions in body weight gain, the Lee index, and body mass index. The serum lipid profiles markedly improved, with decreases in the total cholesterol, triglyceride, and LDL levels, as well as cardiovascular risk indices. Furthermore, fecal analysis showed increased levels of short-chain fatty acids, particularly butyrate. These changes suggest enhanced gut microbial activity; however, the prebiotic effects were inferred from the SCFA profiles, as the gut microbiota composition was not directly analyzed. In conclusion, honey-conjugated HBb-Bps improve gastrointestinal stability and exhibit antioxidant, hypolipidemic, and gut-modulating effects, supporting their potential use as functional ingredients for managing diet-induced metabolic disorders.

Honeybee brood is a nutrient-rich food source containing natural umami-active compounds, such as glutamic acid, aspartic acid, and 5′-nucleotides, which are responsible for its characteristic umami taste. This study aimed to optimize drying conditions to enhance the umami composition and sensory properties of honeybee brood umami powder (HBb-UP). A factorial design was employed to evaluate the effects of drying temperature and time on umami-related amino acids, 5′-nucleotides, and equivalent umami concentration (EUC). Drying temperature and time significantly influenced the formation of umami compounds, with the optimized drying condition (65 °C for 3 h) promoting higher umami composition and improved sensory attributes of HBb-UP. Volatile flavor analysis using GC–MS and an electronic nose revealed a diverse range of aroma compounds contributing to the overall flavor profile. Descriptive sensory evaluation and electronic tongue analysis indicated that umami and saltiness were the dominant taste attributes, accompanied by mild seasoning and fishy notes associated with interactions between amino acids and nucleotides. Principal component analysis demonstrated positive correlations among umami-related amino acids, nucleotides, EUC, and sensory attributes, confirming their combined contribution to taste perception. These findings highlight the potential of optimized HBb-UP as a natural flavor enhancer and functional ingredient for use in sustainable food systems.