This study explores the proteomic composition of follicular fluid (FF) from women undergoing oocyte retrieval for in vitro fertilisation (IVF), with a focus on the effects of polycystic ovary syndrome (PCOS). FF samples were collected from 74 patients, including 34 with PCOS and 40 oocyte donors. Proteomic profiling using machine learning identified significant differences in protein abundance between the PCOS and control groups. Of the 484 quantified proteins, 20 showed significantly altered levels in the PCOS group. Functional annotation and pathway enrichment analysis pointed to the involvement of protease inhibitors and immune-related proteins in the pathophysiology of PCOS, suggesting that inflammation and immune dysregulation may play a key role. Additionally, HDL assembly was identified as a significant pathway, with apolipoprotein-AI (APOA1) and alpha-2-macroglobulin (A2M) as the major proteins involved. Notably, myosin light polypeptide 6 was the most downregulated protein, showing the highest absolute fold change, and may serve as a novel independent biomarker for PCOS.

Ageing populations, mass “baby-free” policies and children born to mothers at the age at which they are biologically expected to become grandmothers are growing problems in most developed societies. Therefore, any opportunity to improve the quality of infertility treatments seems important for the survival of societies. The possibility of indirectly studying the quality of developing oocytes by examining their follicular fluids (hFFs) offers new opportunities for progress in our understanding the processes of final oocyte maturation and, consequently, for predicting the quality of the resulting embryos and personalising their culture. Using mass spectrometry, we studied follicular fluids collected individually during in vitro fertilisation and compared their composition with the quality of the resulting embryos. We analysed 110 follicular fluids from 50 oocyte donors, from which we obtained 44 high-quality, 39 medium-quality, and 27 low-quality embryos. We identified 2182 proteins by Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS) using a TripleTOF 5600+ hybrid mass spectrometer, of which 484 were suitable for quantification. We were able to identify several proteins whose concentrations varied between the follicular fluids of different oocytes from the same patient and between patients. Among them, the most important appear to be immunoglobulin heavy constant alpha 1 (IgA1hc) and dickkopf-related protein 3. The first one is found at higher concentrations in hFFs from which oocytes develop into poor-quality embryos, the other one exhibits the opposite pattern. None of these have, so far, had any specific links to fertility disorders. In light of these findings, these proteins should be considered a primary target for research aimed at developing a diagnostic tool for oocyte quality control and pre-fertilisation screening. This is particularly important in cases where the fertilisation of each egg is not an option for ethical or other reasons, or in countries where it is prohibited by law.

The paper proposes methodology to employ circularly polarized UWB signals for simultaneous measurement of orientation and distance changes between transmitter and receiver. The proposed technique uses the rotational Doppler effect on circularly-polarized pulsed communication. The amplitude of a circularly-polarized signal is immune to polarization misalignment in the presence of rotation, however the phase is subjected to a frequency-invariant shift proportional to the rotation angle. This significantly distorts the pulse shape in the time-domain and can be used for the measurement of the rotated angle. By combining the technique with the well-known localization capability of UWB systems, one can precisely measure not only the distance, but also the orientation. This is demonstrated by both numerical and experimental studies presented in this paper.

We prove that the zeroth Milnor–Thurston homology group coincides with the zeroth singular homology group for Peano continua. Moreover, we show that the canonical homomorphism between these homology theories is not always injective. However, we prove that

A method is proposed to adaptively change the axial-ratio beam of simple circularly-polarized antennas. It is shown, that by varying the phase shift between two orthogonal elements the axial-ratio beam shape can be dynamically adjusted. This allows control of the direction of minimum axial-ratio and the ability to increase or decrease the beamwidth in one of two planes. The method is intended for satellite navigation systems: it can improve the rejection of reflected signals in varying propagation conditions (e.g. urban canyons), while using simple and low-cost circularly-polarized antennas.