Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with a very poor prognosis. Its treatment is hindered by a lack of new therapeutic alternatives and the existence of the blood–brain barrier (BBB), which restricts the access of drugs commonly used in ALS, such as riluzole, to the brain. To overcome these limitations and increase brain targeting, riluzole-loaded nanostructured lipid carriers (NLC) were prepared and functionalized with lactoferrin (Lf), facilitating transport across the BBB by interacting with Lf receptors expressed in the brain endothelium. NLC were characterized with respect to their physicochemical properties (size, zeta potential, polydispersity index) as well as their stability, encapsulation efficiency, morphology, in vitro release profile, and biocompatibility. Moreover, crystallinity and melting behavior were assessed by DSC and PXRD. Nanoparticles exhibited initial mean diameters between 180 and 220 nm and a polydispersity index below 0.3, indicating a narrow size distribution. NLC remained stable over at least 3 months. Riluzole encapsulation efficiency was very high, around 94–98%. FTIR and protein quantification studies confirmed the conjugation of Lf on the surface of the nanocarriers, with TEM images showing that the functionalized NLC presented a smooth surface and uniform spherical shape. An MTT assay revealed that the nanocarriers developed in this study did not cause a substantial reduction in the viability of NSC-34 and hCMEC/D3 cells at a riluzole concentration up to 10 μM, being therefore biocompatible. The results suggest that Lf-functionalized NLC are a suitable and promising delivery system to target riluzole to the brain.

HIV/AIDS stands as a global burden, and vaginal microbicides constitute a promising strategy for topical pre-exposure prophylaxis. Preceding the development of a microbicide containing tenofovir disoproxil fumarate (TDF) and emtricitabine (FTC), in silico and in vitro studies were performed to evaluate the physicochemical characteristics of both drugs, and to study their biophysical impact in lipid model systems. Results from these pre-formulation studies defined hydrogels as adequate vehicles to incorporate TDF-loaded liposomes and FTC. After studying interactions with mucin, zwitterionic liposomes with a mean diameter of 134 ± 13 nm, an encapsulation TDF efficiency of approximately 84%, and a transition temperature of 41 °C were selected. The chosen liposomal formulation was non-cytotoxic to HEC-1-A and CaSki cells, and was able to favor TDF permeation across polysulfone membranes (J_ss = 9.9 μg·cm⁻²·h⁻¹). After the incorporation of TDF-loaded liposomes and FTC in carbomer hydrogels, the drug release profile was sustained over time, reaching around 60% for both drugs within 3–6 h, and best fitting the Weibull model. Moreover, liposomal hydrogels featured pseudoplastic profiles that were deemed suitable for topical application. Overall, the proposed liposomal hydrogels may constitute a promising formulation for the vaginal co-delivery of TDF/FTC.

The $Wtb$ vertex structure and the search for new anomalous couplings is studied using top quark measurements obtained at the LHC, for a center-of-mass energy of 8 TeV. By combining the latest and most precise results on the single top quark production cross section and the measurements of the $W$-boson helicity fractions (${F}_{0}$ and ${F}_{L}$), it is possible to set new limits, at a 95% C.L., on the real and imaginary components of the new couplings. The combination of the LHC observables clearly improves the limits obtained when using the individual results alone. The updated measurements of the $W$-boson helicity fractions and the $s+t$ channels' electroweak single top quark production, at the Tevatron, improve the LHC limits when a world combination of all observables ($\mathrm{LHC}+\text{Tevatron}$) is performed.

Chronic skin exposure to external hostile agents (e.g., UV radiation, microorganisms, and oxidizing chemicals) may increase oxidative stress, causing skin damage and aging. Because of their well-known skincare and protective benefits, quercetin (Q) and omega-3 fatty acids (ω₃) have attracted the attention of the dermocosmetic and pharmaceutical sectors. However, both bioactives have inherent properties that limit their efficient skin delivery. Therefore, nanostructured lipid carriers (NLCs) and enriched PFC^® hydrogels (HGs) have been developed as a dual-approach vehicle for Q and/or ω₃ skin topical administration to improve bioactives’ stability and skin permeation. Two NLC formulations were prepared with the same lipid composition but differing in surfactant composition (NLC1—soy lecithin and poloxamer 407; NLC2—Tween^® 80 and dioctyl sodium sulfosuccinate (DOSS)), which have an impact on physicochemical properties and pharmaceutical and therapeutic performance. Despite both NLCs presenting high Q loading capacity, NLC2′s physicochemical properties make them more suitable for topical skin administration and ensure longer colloidal stability. Additionally, NLC2 demonstrated a more sustained Q release, indicating higher bioactive storage while improving permeability. The occlusive effect of NLCs-enriched HGs also has a positive impact on skin permeability. Q-loaded NLC2, with or without ω₃, -enriched HGs demonstrated efficacy as antioxidant and photoprotective formulations as well as effective reduction in S. aureus growth, indicating that they constitute a promising approach for topical skin administration to prevent skin aging and other damaging cutaneous processes.

Three series of fluorescent phenanthroimidazoles bearing heterocyclic spacers were synthesized in moderate to excellent yields and evaluated as optical chemosensors for ions as well as two-photon absorbing chromophores. Interaction of compounds 5–7 with anions and cations in acetonitrile and acetonitrile/H₂O (95 : 5) showed them to be selective receptors for several anions (AcO⁻, CN⁻ and F⁻) and cations (Fe³⁺, Cu²⁺ and Pd²⁺), with compound 7a being the most sensitive receptor for Fe³⁺ and Cu²⁺. On the other hand, compounds 5a, 7b and 7c were the most sensitive receptors for AcO⁻, CN⁻ and F⁻. The binding stoichiometry between the receptors and the anions and cations was found to be 1 : 2 (ligand to anion/metal cation). The binding process was also followed by ¹H NMR titrations. The evaluation of the TPA properties of chosen phenanthroimidazoles 7a–c by the two-photon induced fluorescence method revealed that compound 7c, which contains a bithienyl spacer, exhibited the highest TPA cross-section (σ₂) value.