Italy supplies approximately 96% of EU-farmed Manila clams. Following a reduction in wild seed availability, farmers started to depend on hatchery-produced seed, mainly imported from other countries. Indeed, only one hatchery is currently operating in Italy. This study quantifies the environmental impacts of seed production in this Italian hatchery facility to inform future planning for improving the sustainability of the supply chain. The environmental performance of the Manila clam hatchery was evaluated using the Life Cycle Assessment methodology. A cradle-to-gate analysis was performed, covering the following production phases: (1) microalgae production, (2) broodstock maintenance and conditioning, and (3) larval rearing until marketable size. The functional unit adopted was 1 kg of live clam seed. The main driver of the environmental impacts was electricity consumption, contributing over 80% for all impact categories. Other inputs showed minor contributions to different impact categories, including liquid oxygen, water pumps, and high-density polyethylene. This study highlights that the environmental burden associated with seed production could be reduced by switching to alternative technologies to meet energy needs, such as investments in photovoltaic and wind energy production systems.

The quality aspects of Ostrea edulis (O. edulis) cultured in Valli di Comacchio were examined across different seasons. Nutritional quality parameters, antioxidant activity, total carotenoids, and contaminants were determined in winter, summer, and autumn (December, June, and October). Seasonal variations in nutritional parameters were observed. In particular, in the winter samples, proteins, eicosapentaenoic acid, docosahexaenoic acid, threonine, tyrosine, valine and methionine, isoleucine, potassium, and carotenoids showed the highest values, whereas oleic acid, linolenic acid, lysine, and magnesium exhibited the maximum values in the summer. Finally, lipids, carbohydrates, histidine, and magnesium were present at high values in the autumn. The antioxidant activity values differed between the two methods used (DPPH and photochemiluminescence assays); however, the oysters collected in June presented lower antioxidant capacity. Contaminant levels were always below the imposed concentration limits; however, higher levels of toxic metals were detected in the winter, while polycyclic aromatic hydrocarbons were detected in the summer and autumn. It is important to monitor the nutritional and toxicological quality of Ostrea edulis throughout the cultivation seasons, not only to enhance knowledge of this species and improve its cultivation systems but also to determine the optimal period for human consumption from both nutritional and toxicological perspectives.

Oyster aquaculture offers promising opportunities for diversifying marine production in the Romanian Black Sea, where favorable environmental conditions and recent regulatory developments support shellfish farming. This study aimed to generate baseline data for the cultivation of the Pacific oyster, Magallana gigas, through an in situ experimental trial conducted off Mamaia Bay, Romania. A 50 m experimental long-line system was deployed at 13.5 m depth, and triploid oysters were cultured for one year at two depth horizons (3 m and 6 m). The growth performance, meat yield, Condition Index, microbiological quality, environmental parameters, and epibiotic communities were monitored monthly. Cultivation depth significantly influenced oyster growth, with individuals reared at 6 m consistently achieving a greater wet weight and shell length than those at 3 m. Growth rates peaked during spring, and meat yield values indicated good commercial quality. Environmental monitoring showed strong seasonal variability, with high summer temperatures and reduced dissolved oxygen associated with increased mortality. Microbiological analyses revealed higher bacterial loads during warm months. The Condition Index classified the oysters as generally “fine” to occasionally “special”. Overall, the results demonstrate that Black Sea conditions can support successful cultivation of M. gigas, although seasonal environmental stress and epibiosis require appropriate farm management.

One of the main threats to aquaculture is represented by microbial pathogens, causing mass mortality episodes in hatcheries, which result in huge economic losses. Among the many disinfection methods applied to reduce this issue, the use of chemicals and beneficial microorganisms (probiotics) seems to be the most efficient. The aim of this study is to test the efficacy of two of them: a chemical, peracetic acid (PAA), and a probiotic, Phaeobacter inhibens. Tests were run on microalgae of the species Isochrysis galbana (var T-ISO). For both remedies, the microalgae survival rate and final cell concentration (cell/mL) were monitored. PAA analysis tested six different concentrations of the chemical: 7.5 µg, 10 µg/L, 20 µg/L, 30 µg/L, 40 µg/L, and 60 µg/L. Meanwhile, P. inhibens was tested with a concentration of 104 CFU/mL. Analysis for both the remedies was conducted on a laboratory scale using glass flasks, and on an industrial scale inside photobioreactors (PBRs). Among all the treatments, the one with PAA dosed with a concentration of 60 µg/L gave the best results, as the culture reached a final density of 8.61 × 10⁶ cell/mL. However, none of the remedies involved in the experiment harmed microalgae or their growth. The results match perfectly with the condition requested for the tested remedies: to obtain an optimal breakdown of pathogens without interfering with culture growth. These features make PAA and P. inhibens good candidates for disinfection methods in aquaculture facilities.