Food insecurity is a prime difficulty in Ethiopia. Food security policies and intervention mechanisms require empirical evidence on factors related to enhancement of food security. This study analyzed the determinants of households’ food security status in pastoral and agro-pastoral districts of Afar regional state. Multistage sampling technique was used to identify sampled households. The sample size of each kebele ¹ 1 Kebele is the smallest administrative hierarchy in Ethiopia. The country is a federal state of regions where every region is structured into zones; zones are divided into districts; every district is again divided into kebeles. View all notes was determined proportionally and selected 150 households. The data were analyzed by descriptive statistics, Foster Greer Thorbecke index and multiple linear regression model. By taking 2100 kcal/AE/day as a cut-off point, 72.7% of the households were found food insecure. The results of multiple linear regression model indicated that family size (adult equivalent) and mothers’ time constraint have a significantly negative effect on food security status at less than 1%. Livestock ownership (TLU) and sex (male) have a significantly positive effect on food security status at less than 1% and 5%, respectively. Fundamentally, efforts from federal and regional government and other stakeholders are needed to improve livestock production, productivity and commercialization. Awareness creation of effective family planning via giving special attention to female-headed households is worthily helpful to maximize food security in the study area.

Embryonic development is one of the most sensitive and critical stages when maternal effects may influence the offspring’s phenotype. In birds and other oviparous species, embryonic development is confined to the eggs, therefore females must deposit resources into the eggs to prepare the offspring for the prevailing post-natal conditions. However, the mechanisms of such phenotypic adjustments remain poorly understood. We simulated a maternal nutritional transfer by injecting 1 mg of l-methionine solution into Japanese quail eggs before the onset of incubation. The increase in early methionine concentration in eggs activated the insulin/insulin-like signalling and mechanistic target of rapamycin (IIS/mTOR) signalling pathways and affected post-natal developmental trajectories. Chicks from methionine-supplemented eggs had higher expression of liver IGF1 and mTOR genes at hatching but were similar in size, and the phenotypic effects of increased growth became apparent only a week later and remained up to three weeks. Circulating levels of insulin-like growth factor-1 (IGF-1) and expression of ribosomal protein serine 6 kinase 1 (RPS6K1), the mTOR downstream effector, were elevated only three weeks after hatching. These results show that specific nutritional cues may have phenotypic programming effects by sequentially activating specific nutrient-sensing pathways and achieving transgenerational phenotypic plasticity.

Limited resources affect an organism’s physiology through the conserved metabolic pathway, the mechanistic target of rapamycin (mTOR). Males and females often react differently to nutritional limitation, but whether it leads to differential mTOR pathway expression remains unknown. Recently, we found that dietary restriction (DR) induced significant changes in the expression of mTOR pathway genes in female Japanese quails (Coturnix japonica). We simultaneously exposed 32 male and female Japanese quails to either 20%, 30%, 40% restriction or ad libitum feeding for 14 days and determined the expression of six key genes of the mTOR pathway in the liver to investigate sex differences in the expression patterns. We found that DR significantly reduced body mass, albeit the effect was milder in males compared to females. We observed sex-specific liver gene expression. DR downregulated mTOR expression more in females than in males. Under moderate DR, ATG9A and RPS6K1 expressions were increased more in males than in females. Like females, body mass in males was correlated positively with mTOR and IGF1, but negatively with ATG9A and RS6K1 expressions. Our findings highlight that sexes may cope with nutritional deficits differently and emphasise the importance of considering sexual differences in studies of dietary restriction.

Resources are needed for growth, reproduction and survival, and organisms must trade off limited resources among competing processes. Nutritional availability in organisms is sensed and monitored by nutrient-sensing pathways that can trigger physiological changes or alter gene expression. Previous studies have proposed that one such signalling pathway, the mechanistic target of rapamycin (mTOR), underpins a form of adaptive plasticity when individuals encounter constraints in their energy budget. Despite the fundamental importance of this process in evolutionary biology, how nutritional limitation is regulated through the expression of genes governing this pathway and its consequential effects on fitness remain understudied, particularly in birds. We used dietary restriction to simulate resource depletion and examined its effects on body mass, reproduction and gene expression in Japanese quails (Coturnix japonica). Quails were subjected to feeding at 20%, 30% and 40% restriction levels or ad libitum for 2 weeks. All restricted groups exhibited reduced body mass, whereas reductions in the number and mass of eggs were observed only under more severe restrictions. Additionally, dietary restriction led to decreased expression of mTOR and insulin-like growth factor 1 (IGF1), whereas the ribosomal protein S6 kinase 1 (RPS6K1) and autophagy-related genes (ATG9A and ATG5) were upregulated. The pattern in which mTOR responded to restriction was similar to that for body mass. Regardless of the treatment, proportionally higher reproductive investment was associated with individual variation in mTOR expression. These findings reveal the connection between dietary intake and the expression of mTOR and related genes in this pathway.

Methionine (Met) is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and growth. The present study examined the effect of in ovo injection of DL-Met and L-Met sources and genotypes on chicken embryonic-intestinal development and health. Fertilized eggs of the two genotypes, TETRA-SL layer hybrid (TSL) - commercial layer hybrid and Hungarian Partridge colored hen breed (HPC) - a native genotype, were randomly distributed into four treatments for each genotype. The treatment groups include the following: 1) control non-injected eggs (NoIn); 2) saline-injected (SaIn); 3) DL-Met injected (DLM); and 4) L-Met injected (LM). The in ovo injection was carried out on 17.5 d of embryonic development; after hatching, eight chicks per group were sacrificed, and the jejunum was extracted for analysis. The results showed that both DLM and LM groups had enhanced intestinal development as evidenced by increased villus width, villus height, and villus area (P < 0.05) compared to the control. The DLM group had significantly reduced crypt depth, glutathione content (GSH), glutathione S-transferase 3 alpha (GST3), occludin (OCLN) gene expression and increased villus height to crypt depth ratio in the TSL genotype than the LM group (P < 0.05). The HPC genotype has overexpressed insulin-like growth factor 1 (IGF1) gene, tricellulin (MD2), occludin (OCLN), superoxide dismutase 1 (SOD1), and GST3 genes than the TSL genotype (P < 0.05). In conclusion, these findings showed that in ovo injection of Met enhanced intestinal development, and function, with genotypes responding differently under normal conditions. Genotypes also influenced the expression of intestinal antioxidants, tight junction, and growth-related genes.

The contribution of beekeeping is perhaps one of the most important income-generating activities for millions of smallholder farmers in Ethiopia. This study was intended to assess beekeeping practices and potential in three districts of Afar Region, northern Ethiopia. Primary data were collected from 120 respondents proportionally selected from each district. Semi-structured questionnaire were employed to collect the primary data. Focus-group discussion was also used to support interpretation of the interview data. Basically, descriptive statistics were used to analyse the data. All respondents use traditional honey production system despite some recent trials. The mean live colony ownership of the sample beekeepers is 10.08 colonies per household, with a maximum ownership of 62 colonies. The study showed that the annual honey production per beehive varies from 4 to 17 kg, with a mean production of 9.66 kg. The majority of the respondents harvest two times per year, while 18%, 19%, and 14.2% of the respondents harvest three, four, and five times per year respectively. This might be due to the special floral calendar of tropical plants found in the areas. 67.5% of respondents supplement their colonies during dry season. Producers travel more than seven kilometres to sell their honey. On average, beekeepers sell 77.86 kg per year individually, with a range of 0 to 353 kg. The main constraints of honey production in the area are recurrent drought, poor extension service, lack of access to improved technology, deforestation, etc. Therefore, it requires intervention to change the old beekeeping practices through training and introducing improved production systems.