The gibberellic acid-stimulated Arabidopsis (GASA) gene family plays a crucial role in growth, development, and stress response, and it is specific to plants. This gene family has been extensively studied in various plant species, and its functional role in pineapple has yet to be characterized. In this study, 15 AcGASA genes were identified in pineapple through a genome-wide scan and categorized into three major branches based on a phylogenetic tree. All AcGASA proteins share a common structural domain with 12 cysteine residues, but they exhibit slight variations in their physicochemical properties and motif composition. Predictions regarding subcellular localization suggest that AcGASA proteins are present in the cell membrane, Golgi apparatus, nucleus, and cell wall. An analysis of gene synteny indicated that both tandem and segmental repeats have a significant impact on the expansion of the AcGASA gene family. Our findings demonstrate the differing regulatory effects of these hormones (GA, NAA, IAA, MeJA, and ABA) on the AcGASA genes. We analyzed the expression profiles of GASA genes in different pineapple tissue parts, and the results indicated that AcGASA genes exhibit diverse expression patterns during the development of different plant tissues, particularly in the regulation of floral organ development. This study provides a comprehensive understanding of GASA family genes in pineapple. It serves as a valuable reference for future studies on the functional characterization of GASA genes in other perennial herbaceous plants.

AP2/ERF (APETALA2/Ethylene Responsive Factor) transcription factors, a class of plant-specific transcription factors, play a pivotal role in plant growth, development, metabolism, and stress response. The pineapple (Ananas comosus (L.) Merr.), a perennial fruit, belongs to the Bromeliaceae family. It is an economically important crop worldwide, which is consumed as fresh fruit, canned fruit, a fiber source, and even pharmaceutical raw material. We identified 75 AcoAP2/ERF genes in the pineapple genome, with four manually curated. They were distributed evenly on 23 chromosomes, except on LG20 and LG23. Sequence lengths, molecular weights, and intron numbers were diverse. The majority of pineapple AcoAP2/ERF genes were localized in nuclear while seven AcoERFs were located in mitochondrial or chloroplast. All pineapple AcoAP2/ERF genes possess an AP2 domain and are divided into 10 clades. Most originate from whole-genome or segmental duplication instead of transposon events. Utilizing pineapple calluses as experimental material, qRT–PCR analysis revealed that the expression of the majority of AcoAP2/ERF genes was induced in response to abscisic acid (ABA), gibberellic acid (GA), ethylene (ET), and naphthalene acetic acid (NAA). In this study, we cloned the promoter sequence of the AcoERF24 gene and divided it into three fragments to construct individual vectors. These vectors were subsequently introduced into Arabidopsis thaliana for β-glucuronidase (GUS) activity analysis, revealing variations in activity levels among the different fragments. This study not only deepens our understanding of the AcoAP2/ERF genes family in olives but also provides an important basis for subsequent studies on the regulation of AcoERF24 gene expression and biological functions.