Cotton, with a cultivated area of 31.92 × 10⁶ ha⁻¹ across 80 countries and an estimated annual turnover of USD 5.68 billion, is the world’s leading natural textile fiber. However, many cotton-producing countries have neglected to improve production practices, adversely affecting the environment and society. A systematic review of the sustainable cotton cultivation literature was performed for the first time to identify and suggest context-specific agricultural strategies that can be applied within different agroecosystems. The key aspects include (1) inoculation with arbuscular mycorrhizal species such as Gigaspora margarita, Funneliformis mosseae, and Acaulospora scrobiculata to enhance root exploration, biomass, and nutrient uptake; (2) using grass, legume, and brassica cover crops as a valid alternative to monoculture and fallow crop rotations to reduce resource depletion and increase the sustainability of cotton production; (3) adopting drip and mulched drip irrigation systems over traditional furrow and sprinkler systems for water conservation; (4) exploring the feasibility of prematurely terminating irrigation in humid subtropical and Mediterranean climates as an alternative to chemical defoliation without affecting cotton yield. This paper, which describes various farming practices adopted in different climates, provides farmers a guide for eco-friendly cotton agronomic management without sacrificing productivity.

This study explores the revival of cotton (Gossypium spp. L.) farming in Italy through sustainable practices, addressing economic and water-related challenges by integrating cultivar selection, arbuscular mycorrhizal fungi (AMF) inoculation, and deficit irrigation under organic farming. Field trials evaluated two widely grown Mediterranean cultivars (Armonia and ST-318) under three irrigation levels (I-100: 100% crop water requirement; I-70: 70%; I-30: 30%) across two Sicilian soil types (sandy loam vs. clay-rich). Under I-100, lint yields reached 0.99 t ha⁻¹, while severe deficit (I-30) yielded only 0.40 t ha⁻¹. However, moderate deficit (I-70) maintained 75–79% of full yields, proving a viable strategy. AMF inoculation significantly enhanced plant height (68.52 cm vs. 65.85 cm), boll number (+22.1%), and seed yield (+12.5%) (p < 0.001). Cultivar responses differed: Armonia performed better under water stress, while ST-318 thrived with full irrigation. Site 1, with higher organic matter, required 31–38% less water and achieved superior irrigation water productivity (1.43 kg m⁻³). Water stress also shortened phenological stages, allowing earlier harvests—important for avoiding autumn rains. These results highlight the potential of combining adaptive irrigation, resilient cultivars, and AMF to restore sustainable cotton production in the Mediterranean, emphasizing the importance of soil-specific management.

Cotton production faces sustainability challenges due to the lack of effective sustainable defoliants for mechanical harvesting, which constrains the expansion of organic cotton (currently 0.5% of global production). In this framework, this study evaluated pelargonic acid, a rapidly biodegradable compound, as a sustainable defoliant alternative, comparing it with the synthetic pyraflufen-ethyl and a water placebo. A two-year field trial (2023–2024) in Sicily, southern Italy, tested three application rates per treatment in a randomized complete block design. Parameters assessed included defoliation efficacy, root diameter, boll number per plant, average boll weight, raw yield, lint yield, and seed yield. Results indicated significant “Year × Treatment” interaction effects on all parameters. Pelargonic acid applied at 16 L ha⁻¹ achieved the highest boll number per plant in 2024, significantly exceeding pyraflufen-ethyl at its label-recommended rate, with treatments at 12 L ha⁻¹ also producing larger root diameters than the synthetic defoliant. Pelargonic acid at 18 L ha⁻¹ in 2023 achieved complete defoliation, matching the efficacy of pyraflufen-ethyl, while the lowest pelargonic rate (12 L ha⁻¹) produced >90% leaf drop across both years. These findings position pelargonic acid as a rapidly degradable alternative to synthetic defoliants, directly addressing a key bottleneck in sustainable cotton production.