Rosemary (Rosmarinus officinalis L.) is an evergreen and a xerophytic shrub that is frequently employed in culinary, decorative, and industrial applications. It shows significant variations in biomass and essential oil (EO) yield due to effects of biotic and abiotic factors. Biostimulants are widely used in agriculture, and in organic agricultural systems, they may contribute significantly to the stability and/or to the increase in crop yields, ensuring respect for the environment. The aim of this study was to assess the effect of different types of biostimulants on the yield performance of rosemary. Four commercial formulations of biostimulants based on Eklonia maxima, Ascophyllum nodosum, fulvic acids, and protein hydrolysates were used for the tests. Water was used as a control. Six applications were performed. At harvest, several morphological and productive parameters were determined. All parameters were affected by biostimulant application. The highest fresh and dry yields were obtained with the application of fulvic acids (13.1 t ha⁻¹ of fresh biomass and 4.3 t ha⁻¹ of dry biomass) and protein hydrolysates (13.6 t ha⁻¹ of fresh biomass and 4.4 t ha⁻¹ of dry biomass) with values 60% higher than that of the control. Regarding the EO content in the dry biomass, the highest value (1.72% v/w) was found in control plants, whilst the lowest (1.14%) was observed in plants treated with fulvic acids. In the case of the EO yield, the lowest value (46.5 kg ha⁻¹) per unit area was found in the control. The application of Eklonia maxima and protein hydrolysates produced the highest EO yield values, 65 kg ha⁻¹ and 66.5 kg ha⁻¹, respectively. This study highlights the use of biostimulants to increase the yield performance of rosemary when grown in an organic agricultural system.

The determination of the actual crop water requirement (CWR) today represents an important prerogative for combating climate change. A three-year trial was conducted to ad-dress the need to provide adequate support to processing tomato growers in defining the correct amounts of water to be supplied. In fact, the objective of this work was to calculate the water requirement of processing tomatoes, specifically analyzing their irrigation needs using the CROPWAT 8.0 software and through capacitive and tensiometric probes. Furthermore, for both methods, the tomato yield was evaluated both by supplying 100% of its water requirement and by supplying, through regulated deficit irrigation (RDI), 70% of its water requirement. Subsequently, for each irrigation strategy employed and for each CWR calculation method, the water footprint was calculated by analyzing the blue, green, and grey components. In the years 2022 and 2023, there was an overestimation of CWR of 13.5% for IR100 and 13.94% for IR70, and 14.53% for IR100 and 11.65% for IR70, respectively, while in 2024 there was an underestimation, with values of 9.17% and 5.22% for the IR100 and IR70 treatments compared to the values obtained with the probes. The total WF of tomatoes varied between 33.42 and 51.91 m³ t⁻¹ with the CROPWAT model and between 35.82 and 47.19 m³ t⁻¹ with the probes for IR100, while for RDI70, the values ranged between 38.72 and 59.44 m³ t⁻¹ with the CROPWAT method and between 35.81 and 53.95 m³ t⁻¹ with the probe method. In water-scarce regions, integrating the CROPWAT 8.0 model (enhanced with real-world data) and implementing smart systems can significantly improve water management, refine decision-making processes, and mitigate environmental impacts. This approach directly addresses the urgent need for water security within sustainable agriculture.

This study explores the revival of cotton (Gossypium spp. L.) farming in Italy through sustainable practices, addressing economic and water-related challenges by integrating cultivar selection, arbuscular mycorrhizal fungi (AMF) inoculation, and deficit irrigation under organic farming. Field trials evaluated two widely grown Mediterranean cultivars (Armonia and ST-318) under three irrigation levels (I-100: 100% crop water requirement; I-70: 70%; I-30: 30%) across two Sicilian soil types (sandy loam vs. clay-rich). Under I-100, lint yields reached 0.99 t ha⁻¹, while severe deficit (I-30) yielded only 0.40 t ha⁻¹. However, moderate deficit (I-70) maintained 75–79% of full yields, proving a viable strategy. AMF inoculation significantly enhanced plant height (68.52 cm vs. 65.85 cm), boll number (+22.1%), and seed yield (+12.5%) (p < 0.001). Cultivar responses differed: Armonia performed better under water stress, while ST-318 thrived with full irrigation. Site 1, with higher organic matter, required 31–38% less water and achieved superior irrigation water productivity (1.43 kg m⁻³). Water stress also shortened phenological stages, allowing earlier harvests—important for avoiding autumn rains. These results highlight the potential of combining adaptive irrigation, resilient cultivars, and AMF to restore sustainable cotton production in the Mediterranean, emphasizing the importance of soil-specific management.

The use of biostimulants in agriculture provides a sustainable and efficient technology to improve resource‐use efficiency. Biostimulants may boost vegetative growth, enhancing plant tolerance to biotic and abiotic stress. The tomato (Solanum lycopersicum L.) is sensitive to drought stress, particularly during fruit setting and fruit development stages. In Italy, long‐storage tomato genotypes characterised by drought resistance were selected. In this 2‐year study, the foliar application of different biostimulants (betaine, seaweed extracts, vegetal protein hydrolysate and animal protein hydrolysate) was evaluated to determine effects on yield and quality of a local tomato landrace (Pizzutello) cultivated in Sicily without irrigation. The highest dry matter (9.9%) and solid soluble content (6.9° Brix) were observed in plants treated with betaine. Plants treated with A. nodosum or animal protein hydrolysate showed the highest potassium concentrations, whereas those supplied with vegetal protein hydrolysate had the highest calcium concentrations. Tomato treated with betaine were found to have the highest nitrate concentrations. The highest marketable yield (13.8 t ha⁻¹) was recorded in plants treated with vegetal protein hydrolysate, with an increase of 17.4% compared to the control plants. The highest unmarketable yield was observed in control plants and in those treated with betaine (1.1 t ha^‐1). In conclusion, we can say that each biostimulant had a different effect on the different parameters analysed. Overall, the application of biostimulants has improved tomato growth, productivity and quality in limited water conditions. Our results highlight the potential of biostimulant applications to optimise both the yield and fruit quality of renowned local varieties. This study demonstrated the improvement in the agronomic performance of the Pizzutello tomato, which is particularly significant not only in response to the growing consumer demand for high‐quality traditional tomatoes, but also for the enhancement of the technological traits valued by the food industry.

Sugarcane (Saccharum spp. L.), traditionally cultivated in tropical and subtropical regions, is being explored for its agronomic viability in Mediterranean climates. This study assessed the bio-agronomic performance of seven sugarcane varieties and two accessions grown in Sicily, to enhance the fermentation process to produce rum agricole, a spirit derived from fresh cane juice. Agronomic evaluations revealed significant varietal differences, with juice yields of 5850−14,312 L ha⁻¹ and sugar yields of 1.84–5.33 t ha⁻¹. Microbial control was achieved through the addition of lactic acid, which effectively suppressed undesirable bacterial growth and improved fermentation quality. Furthermore, the application of two selected Saccharomyces cerevisiae strains (MN113 and SPF21), isolated from high-sugar matrices such as manna and honey byproducts, affected the production of volatile compounds, particularly esters and higher alcohols. Sensory analysis confirmed a more complex aromatic profile in cane wines fermented with these selected yeasts, with overall acceptance scores reaching 7.5. Up to 29 aroma-active compounds were identified, including ethyl esters and higher alcohols. This research represents the first integrated approach combining lactic acid treatment and novel yeast strains for the fermentation of sugarcane juice in a Mediterranean context. The findings highlight the potential for high-quality rum agricole production in Sicily.

Reducing synthetic fertilizer inputs in durum wheat while maintaining crop performance is a key challenge for Mediterranean cereal systems, where soil constraints frequently limit nutrient availability and canopy function. This study evaluated the physiological and soil‐nutrient responses of durum wheat ( Triticum turgidum subsp. durum cv. Antalis) to a multi‐strain Bacillus consortium ( B. licheniformis , B. methylotrophicus , B. megaterium ) applied through different delivery strategies under fertilized and unfertilized field conditions. Field trials were conducted during the 2023–2024 season at two contrasting Sicilian sites (Campo Carboj and Sparacia) using a randomized complete block design with three replicates. Treatments included seed coating, soil spraying at three rates and combined seed + soil applications. Post‐harvest soil available P (Olsen, expressed as P ₂ O ₅ ) and plant traits were assessed, alongside physiological indicators of canopy function (SPAD chlorophyll index, stomatal conductance [G _s ] and NDVI across key BBCH stages). Across both sites, microbial inoculation increased soil P ₂ O ₅ availability, with the strongest responses at Sparacia (clay‐rich, more P‐immobilizing conditions), where several microbial treatments produced increases comparable to mineral fertilization and, in some cases, exceeded +50% relative to the unfertilized control. Microbial application also enhanced crop physiology, particularly at Sparacia: non‐fertilized treatments increased plant height (up to +23%), sustained higher SPAD values, improved G _s and increased NDVI from tillering through grain filling, indicating improved canopy vigour under nutrient limitation. Responses at Campo Carboj were generally smaller but consistently positive. Combined seed + soil applications (notably S6–S7) provided the most robust improvements, supporting the agronomic value of multi‐modal inoculation strategies. Overall, the consortium partially compensated for fertilizer withdrawal by improving soil P availability and sustaining physiological performance , with the magnitude varying by site and driven by edaphic context.