Ice hockey is an intense team sport characterized by repeated bursts of fast‐paced skating, rapid changes in speed and direction and frequent physical encounters. These are performed in on‐ice shifts of ~30–80 s interspersed with longer sequences of passive recovery, resulting in about 15–25 min on‐ice time per player. Nearly 50% of the distance is covered at high‐intensity skating speeds and with an accentuated intense activity pattern in forwards compared to defensemen. During ice hockey match‐play, both aerobic and anaerobic energy systems are significantly challenged, with the heart rate increasing toward maximum levels during each shift, and with great reliance on both glycolytic and phosphagen ATP provision. The high‐intensity activity pattern favors muscle glycogen as fuel, leading to pronounced reductions despite the relatively brief playing time, including severe depletion of a substantial proportion of individual fast‐ and slow‐twitch fibers. Player‐tracking suggests that the ability to perform high‐intensity skating is compromised in the final stages of a game, which is supported by post‐game reductions in repeated‐sprint ability. Muscle glycogen degradation, in particular in individual fibers, as well as potential dehydration and hyperthermia, may be prime candidates implicated in exacerbated fatigue during the final stages of a game, whereas multiple factors likely interact to impair exercise tolerance during each shift. This includes pronounced PCr degradation, with potential inadequate resynthesis in a proportion of fast‐twitch fibers in situations of repeated intense actions. Finally, the recovery pattern is inadequately described, but seems less long‐lasting than in other team sports.

Purpose: This study evaluated how extended match time (90 + 30 min) affected physiological responses and fatigue in male soccer players. Methods: Twenty competitive players (mean ± SD: age, 20 ± 1 yr; maximal oxygen uptake, 59 ± 4 mL·min⁻¹·kg⁻¹) completed an experimental match with their activity pattern and heart rate assessed throughout the game, whereas countermovement jump performance and repeated sprint ability were tested and quadriceps muscle biopsies and venous blood samples were taken at baseline and after 90 and 120 min of match play. Results: Less high-intensity running (12%) was performed in extra time in association with fewer intense accelerations and decelerations per minute compared with normal time. Peak sprint speed was 11% lower in extra time compared with normal time, and fatigue also manifested in impaired postmatch repeated sprint ability and countermovement jump performance (all P < 0.05). Muscle glycogen declined from 373 ± 59 mmol·kg⁻¹ dry weight (dw) at baseline to 266 ± 64 mmol·kg⁻¹ dw after 90 min, with a further decline to 186 ± 56 mmol·kg⁻¹ dw after extra time (P < 0.05) and with single-fiber analyses revealing depleted or very low glycogen levels in ~75% of both slow and fast twitch fibers. Blood glucose did not change during the first 90-min but declined (P < 0.05) to 81 ± 8 mg·dL⁻¹ after extra time. Plasma glycerol and ammonia peaked at 236 ± 33 mg·dL⁻¹ and 75 ± 21 μmol·L⁻¹ after the extra period. Conclusions: These findings demonstrate exacerbated fatigue after extra time compared with normal time, which seems to be associated with muscle glycogen depletion, reductions in blood glucose levels, and hyperammonemia. Together, this points to metabolic disturbances being a major part of the integrated and multifaceted fatigue response during extended soccer match play.

Purpose: We investigated the coupling between muscle glycogen content and localization and high-intensity exercise performance using a randomized, placebo-controlled, parallel-group design with emphasis on single-fiber subcellular glycogen concentrations and sarcoplasmic reticulum Ca²⁺ kinetics. Methods: Eighteen well-trained participants performed high-intensity intermittent glycogen-depleting exercise, followed by randomization to a high- (CHO; ~1 g CHO·kg⁻¹·h⁻¹; n = 9) or low-carbohydrate placebo diet (PLA, <0.1 g CHO·kg⁻¹·h⁻¹; n = 9) for a 5-h recovery period. At baseline, after exercise, and after the carbohydrate manipulation assessments of repeated sprint ability (5 × 6-s maximal cycling sprints with 24 s of rest), neuromuscular function and ratings of perceived exertion during standardized high-intensity cycling (~90% W _max) were performed, while muscle and blood samples were collected. Results: The exercise and carbohydrate manipulations led to distinct muscle glycogen concentrations in CHO and PLA at the whole-muscle (291 ± 78 vs 175 ± 100 mmol·kg⁻¹ dry weight (dw), P = 0.020) and subcellular level in each of three local regions (P = 0.001–0.046). This was coupled with near-depleted glycogen concentrations in single fibers of both main fiber types in PLA, especially in the intramyofibrillar region (within the myofibrils). Furthermore, increased ratings of perceived exertion and impaired repeated sprint ability (~8% loss, P < 0.001) were present in PLA, with the latter correlating moderately to very strongly (r = 0.47–0.71, P = 0.001–0.049) with whole-muscle glycogen and subcellular glycogen fractions. Finally, sarcoplasmic reticulum Ca²⁺ uptake, but not release, was superior in CHO, whereas neuromuscular function, including prolonged low-frequency force depression, was unaffected by dietary manipulation. Conclusions: Together, these results support an important role of muscle glycogen availability for high-intensity exercise performance, which may be mediated by reductions in single-fiber levels, particularly in distinct subcellular regions, despite only moderately lowered whole-muscle glycogen concentrations.

Glycogen particles are situated in key areas of the muscle cell in the vicinity of the main energy‐consumption sites and may be utilised heterogeneously dependent on the nature of the metabolic demands. The present study aimed to investigate the time course of fibre type‐specific utilisation of muscle glycogen in three distinct subcellular fractions (intermyofibrillar, IMF; intramyofibrillar, Intra; and subsarcolemmal, SS) during repeated high‐intensity intermittent exercise. Eighteen moderately to well‐trained male participants performed three periods of 10 × 45 s cycling at ∼105% watt max (EX1–EX3) coupled with 5 × 6 s maximal sprints at baseline and after each period. Muscle biopsies were sampled at baseline and after EX1 and EX3. A higher glycogen breakdown rate in type 2 compared to type 1 fibres was found during EX1 for the Intra (−72 vs. −45%) and IMF (−59 vs. −35%) glycogen fractions (P < 0.001) but with no differences for SS glycogen (−52 vs. −40%). In contrast, no fibre type differences were observed during EX2–EX3, where the utilisation of Intra and IMF glycogen in type 2 fibres was reduced, resulting in depletion of all three subcellular fractions to very low levels post‐exercise within both fibre types. Importantly, large heterogeneity in single‐fibre glycogen utilisation was present with an early depletion of especially Intra glycogen in individual type 2 fibres. In conclusion, there is a clear fibre type‐ and localisation‐specific glycogen utilisation during high‐intensity intermittent exercise, which varies with time course of exercise and is characterised by exacerbated pool‐specific glycogen depletion at the single‐fibre level. image Key points: Muscle glycogen is the major fuel during high‐intensity exercise and is stored in distinct subcellular areas of the muscle cell in close vicinity to the main energy consumption sites. In the present study quantitative electron microscopy imaging was used to investigate the utilisation pattern of three distinct subcellular muscle glycogen fractions during repeated high‐intensity intermittent exercise. It is shown that the utilisation differs dependent on fibre type, subcellular localisation and time course of exercise and with large single‐fibre heterogeneity. These findings expand on our understanding of subcellular muscle glycogen metabolism during exercise and may help us explain how reductions in muscle glycogen can attenuate muscle function even at only moderately lowered whole‐muscle glycogen concentrations.

The aim of the study was to compare the activity pattern, heart rate (HR), technical involvement, and subjective perceptions in U13 boys and girls playing team handball in five game formats. Activity pattern, heart rate (HR), technical involvement, perceived fun, and exertion were recorded from four girls teams (n = 24) and four boys teams (n = 24) played during a 1-day tournament consisting of five different game formats of 15-min duration: Medium court size, 4v4 (M4v4), 5v5 (M5v5), and 6v6 (M6v6), and large court size, 5v5 (L5v5) and 6v6 (L6v6). Girls covered more total distance (TD) and high-speed running (HSR, 13–17.9 km·h⁻¹) on the large court compared to the medium court (p < 0.05; ES = 2.1–3.1 and 1.2–2.5, respectively). Boys covered more distance as HSR and sprinting on the large court compared to the medium court, but only more TD on the large court compared to the medium court with the same number of players, (p < 0.05; ES = 1.0–1.8, 1.0–1.8, and 1.1–1.8, respectively). Team handball for U13 boys and girls is a high-intensity activity irrespective of court size. Increasing the court size with a fixed number of players increased the total distance and HSR, whereas manipulating the number of players on a fixed court size appears to influence technical involvement.

Article: The relationship between age and fitness profiles in elite male ice hockey players - The Journal of Sports Medicine and Physical Fitness 2021 April;61(4):512-8 - Minerva Medica - Journals