Optical Coherence Tomography (OCT) plays a crucial role in diagnosing ocular diseases, yet conventional CNN-based models face limitations such as high computational overhead, noise sensitivity, and data imbalance. This paper introduces HDL-ACO, a novel Hybrid Deep Learning (HDL) framework that integrates Convolutional Neural Networks with Ant Colony Optimization (ACO) to enhance classification accuracy and computational efficiency. The proposed methodology involves pre-processing the OCT dataset using discrete wavelet transform and ACO-optimized augmentation, followed by multiscale patch embedding to generate image patches of varying sizes. The hybrid deep learning model leverages ACO-based hyperparameter optimization to enhance feature selection and training efficiency. Furthermore, a Transformer-based feature extraction module integrates content-aware embeddings, multi-head self-attention, and feedforward neural networks to improve classification performance. Experimental results demonstrate that HDL-ACO outperforms state-of-the-art models, including ResNet-50, VGG-16, and XGBoost, achieving 95% training accuracy and 93% validation accuracy. The proposed framework offers a scalable, resource-efficient solution for real-time clinical OCT image classification.

This proposed work implements various classification approaches to classify the H&E-stained Follicular Lymphoma tissue sample. As part of the process, k-means clustering is used to isolate cytological components like the nucleus extracellular and cytoplasmic regions from images. Then feature space vector is constructed by combining global feature extraction techniques like LBP, LDP, GLCM, and other local feature-extraction techniques. To classify FL images into their respective grades, feature space vectors obtained from feature extraction algorithms are input into a multiclass SVM. Moreover, classification accuracies were explicitly tested with different classifiers like CNN and other pre-trained deep learning networks that can directly operate on raw images without any preprocessing to classify the FL images into their respective grades. The efficacy of different classifiers is presented. This preliminary work provides a proof of concept for incorporating automated FL tissue diagnostic systems into future pathology workflows to supplement the pathologists'.

This paper proposes a noble image segment technique to differentiate between large malignant cells called centroblasts vs. centrocytes. A new approach is introduced, which will provide additional input to an oncologist to ease the prognosis. Firstly, a H&E-stained image is projected onto L*a*b* color space to quantify the visual differences. Secondly, this transformed image is segmented with the help of k-means clustering into its three cytological components (i.e., nuclei, cytoplasm, and extracellular), followed by pre-processing techniques in the third step, where adaptive thresholding and the area filling function are applied to give them proper shape for further analysis. Finally, the demarcation process is applied to pre-processed nuclei based on the local fitting criterion function for image intensity in the neighborhood of each point. Integration of these local neighborhood centers leads us to define the global criterion of image segmentation. Unlike active contour models, this technique is independent of initialization. This paper achieved 92% sensitivity and 88.9% specificity in comparing manual vs. automated segmentation.

Recent trends with Histopathological imagery led to rapid progress towards quantifying the perceptive issues, while prognostic, due to subjective variability among readers. This variability leads to distinguished prognosis reports and generates variability in treatment as well. Latest advancements in image analysis tools have allowed the powerful computer-assisted diagnostic system to assist oncologist in their diagnosis process on radiological data. In this study, we present a thoughtful analysis of texture heterogeneity and morphological characteristic on various lymphomas like Follicular, Neuroblastoma, Breast, and Prostate tissue images for classifying them into respective grades. The study presents a systematic survey of the computational steps of these Lymphomas, which includes recent scenario of diagnosis process to classify these lymphomas into respective grades along with its limitations, followed by it shows the pre-requisite of the computer-assisted diagnosis system and finally explains various segmentation techniques based on image descriptor and subsequent classification of biopsy into respective grades. This paper reviews recent state of the art technology i.e. Computer Assisted Diagnosis (CAD) for Histopathology and also briefly describes the recent development in histology and its application towards quantifying the perceptive issue in the domain of histopathology being pursued in the United State and India.

At now, the majority of approaches rely on manual techniques for annotating cell types subsequent to clustering the data obtained from single-cell RNA sequencing (scRNA-seq). These approaches require a significant amount of physical exertion and depend substantially on the user's skill, perhaps resulting in uneven outcomes and inconsistency in treatment. In this paper, we provide a computer-assisted interpretation of every single cell of a tissue sample, along with an in-depth exploration of an individual cell's molecular, phenotypic and functional attributes. The paper will also perform k-means clustering followed by silhouette validation based on similar phenotype and functional attributes, and also, cell type annotation is performed, where we match a cell's gene profile against some known database by applying certain statistical conditions. Finally, all the genes are mapped spatially on the tissue sample. This paper is an aid to medicine to know which cells are expressed/not expressed in a tissue sample and their spatial location on the tissue sample.