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Protein crystallization still remains mostly an empirical science, as the production of crystals with the required quality for X-ray analysis is dependent on the intensive screening of the best protein crystallization and crystal’s derivatization conditions. Herein, this demanding step was addressed by the development of a high-throughput and low-budget microfluidic platform consisting of an ion exchange membrane (117 Nafion® membrane) sandwiched between a channel layer (stripping phase compartment) and a wells layer (feed phase compartment) forming 75 independent micro-contactors. This microfluidic device allows for a simultaneous and independent screening of multiple protein crystallization and crystal derivatization conditions, using Hen Egg White Lysozyme (HEWL) as the model protein and Hg2+ as the derivatizing agent. This microdevice offers well-regulated crystallization and subsequent crystal derivatization processes based on the controlled transport of water and ions provided by the 117 Nafion® membrane. Diffusion coefficients of water and the derivatizing agent (Hg2+) were evaluated, showing the positive influence of the protein drop volume on the number of crystals and crystal size. This microfluidic system allowed for crystals with good structural stability and high X-ray diffraction quality and, thus, it is regarded as an efficient tool that may contribute to the enhancement of the proteins’ crystals structural resolution.
M. Polino; H. Rho; M. Pina; R. Mallada; A. Carvalho; M. Romão; Isabel Coelhoso; J. Gardeniers; J. Crespo; Carla Portugal. Protein Crystallization in a Microfluidic Contactor with Nafion®117 Membranes. Membranes 2021, 11, 549 .
AMA StyleM. Polino, H. Rho, M. Pina, R. Mallada, A. Carvalho, M. Romão, Isabel Coelhoso, J. Gardeniers, J. Crespo, Carla Portugal. Protein Crystallization in a Microfluidic Contactor with Nafion®117 Membranes. Membranes. 2021; 11 (8):549.
Chicago/Turabian StyleM. Polino; H. Rho; M. Pina; R. Mallada; A. Carvalho; M. Romão; Isabel Coelhoso; J. Gardeniers; J. Crespo; Carla Portugal. 2021. "Protein Crystallization in a Microfluidic Contactor with Nafion®117 Membranes." Membranes 11, no. 8: 549.
There is an increasing demand from the cosmetic, pharmaceutical and food industries for naturally sourced ferulic acid. This study used alkaline extraction under mild operating conditions to recover ferulic acid from corn fibre, with the aim of developing a cost-effective process. The aim was to develop an efficient process of mild alkaline extraction, with an efficient balance between the maximum concentration of ferulic acid and its recovery yield, while producing low-viscous extracts. Alkaline extraction experiments were performed at different scales (laboratory, semi-pilot and pilot scales) to select various operating conditions (type of raw material, concentration of NaOH, liquid-to-solid ratio, time and temperature of extraction) involved in this process. The optimised mild alkaline extraction conditions obtained were: wet corn fibre, without milling and drying, as a starting material followed by extraction with an alkaline solution of NaOH with a concentration of 0.25 M, use of a liquid-to-solid ratio of 10.0 g(extracting solution+ water in the fibre) g−1dry corn fibre, during a period of 7 h at a temperature of 30 ºC. A low-cost, efficient extraction process of ferulic acid from corn fibre (without any pre-treatment) was developed at pilot scale. The resultant extract had ferulic acid concentration and recovery yield, respectively of 2.10 ± 0.09 gferulic acid.L−1 and 11.14 ± 1.00 Kgferulic acid Ton−1dry corn fibre.
Rita Valério; Mafalda Cadima; João G. Crespo; Carla Brazinha. Extracting Ferulic Acid from Corn Fibre Using Mild Alkaline Extraction: A Pilot Scale Study. Waste and Biomass Valorization 2021, 1 -11.
AMA StyleRita Valério, Mafalda Cadima, João G. Crespo, Carla Brazinha. Extracting Ferulic Acid from Corn Fibre Using Mild Alkaline Extraction: A Pilot Scale Study. Waste and Biomass Valorization. 2021; ():1-11.
Chicago/Turabian StyleRita Valério; Mafalda Cadima; João G. Crespo; Carla Brazinha. 2021. "Extracting Ferulic Acid from Corn Fibre Using Mild Alkaline Extraction: A Pilot Scale Study." Waste and Biomass Valorization , no. : 1-11.
Ferulic acid may be used as a nutraceutical ingredient or as a substrate to produce bio-vanillin. There is an increasing market demand for ferulic acid obtained from natural sources such as low-cost agro-industrial by-products, due to its potential applications as nutraceutical ingredient and as a substrate to produce bio-vanillin. This work aims to study ferulic acid recovery from corn fibre (one of the most abundant natural sources of ferulic acid), involving an integrated process of hydrothermal pre-treatment followed by enzymatic hydrolysis. The objective is primarily to produce natural extracts with a maximum ferulic acid recovery yield, but it is also to assess their antioxidant and antiproliferative properties and their cytotoxicity. Different commercial enzyme preparations were tested for release of ferulic acid from corn fibre. The best results were obtained for Ultraflo ®XL in a concentration of 2 % (wenzyme preparation/w dry corn fibre) at a pH of 5 and at 55 °C, presenting a recovery yield of esterified ferulic acid of 7.83 ± 1.35 % (wrecovered ferulic acid/wtotal esterified ferulic acid), which corresponds to 0.13 ± 0.02 % (wferulic acid/w dry corn fibre). When using a hydrothermal pre-treatment at a temperature of 140 °C for 40 min, prior to the use of the same enzymatic hydrolysis procedure, the recovery yield of esterified ferulic acid increased to 28.94 ± 2.40 % (w recovered ferulic acid/ wtotal esterified ferulic acid), which corresponds to 4.9 ± 0.3 % (wferulic acid/wdry corn fibre). The use of this pre-treatment leads not only to the highest yield of ferulic acid, but also to the lowest concentration of furfural and hydroxymethylfurfural, without the formation of formic and levulinic acid (not detected). All pre-treatments tested led to an improved quality of the extract in terms of bioactivity.
Rita Valério; Ana Teresa Serra; João Baixinho; Martim Cardeira; Naiara Fernández; Maria Rosário Bronze; Luís C. Duarte; Maria L. Tavares; João G. Crespo; Carla Brazinha. Combined hydrothermal pre-treatment and enzymatic hydrolysis of corn fibre: Production of ferulic acid extracts and assessment of their antioxidant and antiproliferative properties. Industrial Crops and Products 2021, 170, 113731 .
AMA StyleRita Valério, Ana Teresa Serra, João Baixinho, Martim Cardeira, Naiara Fernández, Maria Rosário Bronze, Luís C. Duarte, Maria L. Tavares, João G. Crespo, Carla Brazinha. Combined hydrothermal pre-treatment and enzymatic hydrolysis of corn fibre: Production of ferulic acid extracts and assessment of their antioxidant and antiproliferative properties. Industrial Crops and Products. 2021; 170 ():113731.
Chicago/Turabian StyleRita Valério; Ana Teresa Serra; João Baixinho; Martim Cardeira; Naiara Fernández; Maria Rosário Bronze; Luís C. Duarte; Maria L. Tavares; João G. Crespo; Carla Brazinha. 2021. "Combined hydrothermal pre-treatment and enzymatic hydrolysis of corn fibre: Production of ferulic acid extracts and assessment of their antioxidant and antiproliferative properties." Industrial Crops and Products 170, no. : 113731.
This work aimed to produce bionanocomposites of chitosan incorporated with zinc oxide nanoparticles (ZnO NPs) synthesized using food industry by-products and to characterize them. Such nanoparticles are highlighted due to their low cost, antimicrobial activity, accessibility, and sustainability synthesis. Four different levels of ZnO NPs (0, 0.5, 1.0, and 2.0% w/w of chitosan) were tested, and the bionanocomposites were characterized in terms of their hydrophobicity, mechanical, optical, and barrier properties. Overall, the incorporation of ZnO NPs changed the composites from brittle to ductile, with enhanced elongation at break and reduced Young Modulus and tensile strength. Thus, ZnO NPs acted as plasticizer, turning the films more flexible, due to the presence of organic compounds on the NPs. This also favored permeability of oxygen and of water vapor, but the good barrier properties were maintained. Optical properties did not change statistically with the ZnO NPs incorporation. Thus, the characterization presented in this paper may contribute to support a decision on the choice of the material’s final application.
Victor Souza; Marta Alves; Catarina Santos; Isabel Ribeiro; Carolina Rodrigues; Isabel Coelhoso; Ana Fernando. Biodegradable Chitosan Films with ZnO Nanoparticles Synthesized Using Food Industry By-Products—Production and Characterization. Coatings 2021, 11, 646 .
AMA StyleVictor Souza, Marta Alves, Catarina Santos, Isabel Ribeiro, Carolina Rodrigues, Isabel Coelhoso, Ana Fernando. Biodegradable Chitosan Films with ZnO Nanoparticles Synthesized Using Food Industry By-Products—Production and Characterization. Coatings. 2021; 11 (6):646.
Chicago/Turabian StyleVictor Souza; Marta Alves; Catarina Santos; Isabel Ribeiro; Carolina Rodrigues; Isabel Coelhoso; Ana Fernando. 2021. "Biodegradable Chitosan Films with ZnO Nanoparticles Synthesized Using Food Industry By-Products—Production and Characterization." Coatings 11, no. 6: 646.
Corn fiber from the corn starch industry is a by-product produced in large quantity that is mainly used in animal feed formulations, though it is still rich in valuable components, such as arabinoxylans, with proven film-forming ability. During arabinoxylans’ recovery under alkaline extraction, a dark-colored biopolymer fraction is obtained. In this work, a purified arabinoxylan extract from corn fiber with an intense brownish color was decolorized using hydrogen peroxide as the decolorizing agent. Biodegradable films prepared by casting the decolorized extract exhibited a light-yellow color, considered more appealing, envisaging their application in food packaging. Films were prepared with glycerol as plasticizer and citric acid as cross-linker. Although the cross-linking reaction was not effective, films presented antioxidant activity, a water vapor permeability similar to that of non-decolorized films, and other polysaccharides’ and mechanical properties that enable their application as packaging materials of low-water-content food products.
Verónica Weng; Carla Brazinha; Isabel Coelhoso; Vitor Alves. Decolorization of a Corn Fiber Arabinoxylan Extract and Formulation of Biodegradable Films for Food Packaging. Membranes 2021, 11, 321 .
AMA StyleVerónica Weng, Carla Brazinha, Isabel Coelhoso, Vitor Alves. Decolorization of a Corn Fiber Arabinoxylan Extract and Formulation of Biodegradable Films for Food Packaging. Membranes. 2021; 11 (5):321.
Chicago/Turabian StyleVerónica Weng; Carla Brazinha; Isabel Coelhoso; Vitor Alves. 2021. "Decolorization of a Corn Fiber Arabinoxylan Extract and Formulation of Biodegradable Films for Food Packaging." Membranes 11, no. 5: 321.
Corn fibre, a co-product of the starch industry, is rich in compounds with high added value, such as ferulic acid and arabinoxylans, which are released during alkaline extraction. This work aims to optimise an efficient separation method for the recovery of these two compounds from a corn fibre alkaline extract, allowing an efficient valorisation of this co-product. Ultrafiltration was selected as separation method, due to its potential to fractionate these compounds. In order to minimise the loss of membrane permeance, due to mass transfer limitations caused by the high arabinoxylan viscosity, the impact of relevant ultrafiltration operating parameters (membrane molecular weight cut-off, fluid dynamics conditions, transmembrane pressure, and operating temperature) were evaluated. A Nadir UP 150 membrane was found to be an adequate choice, allowing for an efficient separation of ferulic acid from arabinoxylans, with null rejection of ferulic acid, a high estimated rejection of arabinoxylans 98.0% ± 1.7%, and the highest permeance of all tested membranes. A response surface methodology (RSM) was used to infer the effect of ultrafiltration conditions (crossflow velocity, transmembrane pressure and operating temperature) on the rejection of ferulic acid, retention of arabinoxylans (assessed through apparent viscosity of the retentate stream), and permeance. Through mathematical modelling it was possible to determine that the best conditions are the highest operating temperature and initial crossflow velocity tested (66 °C and 1.06 m.s−1, respectively), and the lowest transmembrane pressure tested (0.7 bar).
Rita Valério; João Crespo; Claudia Galinha; Carla Brazinha. Effect of Ultrafiltration Operating Conditions for Separation of Ferulic Acid from Arabinoxylans in Corn Fibre Alkaline Extract. Sustainability 2021, 13, 4682 .
AMA StyleRita Valério, João Crespo, Claudia Galinha, Carla Brazinha. Effect of Ultrafiltration Operating Conditions for Separation of Ferulic Acid from Arabinoxylans in Corn Fibre Alkaline Extract. Sustainability. 2021; 13 (9):4682.
Chicago/Turabian StyleRita Valério; João Crespo; Claudia Galinha; Carla Brazinha. 2021. "Effect of Ultrafiltration Operating Conditions for Separation of Ferulic Acid from Arabinoxylans in Corn Fibre Alkaline Extract." Sustainability 13, no. 9: 4682.
Intelligent food packaging is emerging as a novel technology, capable of monitoring the quality and safety of food during its shelf-life time. This technology makes use of indicators and sensors that are applied in the packaging and that detect changes in physiological variations of the foodstuffs (due to microbial and chemical degradation). These indicators usually provide information, e.g., on the degree of freshness of the product packed, through a color change, which is easily identified, either by the food distributor and the consumer. However, most of the indicators that are currently used are non-renewable and non-biodegradable synthetic materials. Because there is an imperative need to improve food packaging sustainability, choice of sensors should also reflect this requirement. Therefore, this work aims to revise the latest information on bio-based sensors, based on compounds obtained from natural extracts, that can, in association with biopolymers, act as intelligent or smart food packaging. Its application into several perishable foods is summarized. It is clear that bioactive extracts, e.g., anthocyanins, obtained from a variety of sources, including by-products of the food industry, present a substantial potential to act as bio-sensors. Yet, there are still some limitations that need to be surpassed before this technology reaches a mature commercial stage.
Carolina Rodrigues; Victor Souza; Isabel Coelhoso; Ana Fernando. Bio-Based Sensors for Smart Food Packaging—Current Applications and Future Trends. Sensors 2021, 21, 2148 .
AMA StyleCarolina Rodrigues, Victor Souza, Isabel Coelhoso, Ana Fernando. Bio-Based Sensors for Smart Food Packaging—Current Applications and Future Trends. Sensors. 2021; 21 (6):2148.
Chicago/Turabian StyleCarolina Rodrigues; Victor Souza; Isabel Coelhoso; Ana Fernando. 2021. "Bio-Based Sensors for Smart Food Packaging—Current Applications and Future Trends." Sensors 21, no. 6: 2148.
The continuous petroleum-based plastics manufacturing generates disposal issues, spreading the problem of plastic pollution and its rise in the environment. Recently, innovative techniques and scientific research promoted biopolymers as the primary alternative for traditional plastics, raising and expanding global bioplastic production. Due to its unmatched biological and functional attributes, chitosan (Ch) has been substantially explored and employed as a biopolymeric matrix. Nevertheless, the hydrophilicity and the weak mechanical properties associated with this biopolymer represent a significant intrinsic restriction to its implementation into some commercial applications, namely, in food packaging industries. Distinct methodologies have been utilized to upgrade the mechanical and barrier properties of Ch, such as using organic or inorganic nanofillers, crosslinkers, or blends with other polymers. This review intends to analyze the most recent works that combine the action of different nanoparticle types with Ch films to reinforce their mechanical and barrier properties.
João Pires; Camila Paula; Victor Souza; Ana Fernando; Isabel Coelhoso. Understanding the Barrier and Mechanical Behavior of Different Nanofillers in Chitosan Films for Food Packaging. Polymers 2021, 13, 721 .
AMA StyleJoão Pires, Camila Paula, Victor Souza, Ana Fernando, Isabel Coelhoso. Understanding the Barrier and Mechanical Behavior of Different Nanofillers in Chitosan Films for Food Packaging. Polymers. 2021; 13 (5):721.
Chicago/Turabian StyleJoão Pires; Camila Paula; Victor Souza; Ana Fernando; Isabel Coelhoso. 2021. "Understanding the Barrier and Mechanical Behavior of Different Nanofillers in Chitosan Films for Food Packaging." Polymers 13, no. 5: 721.
Low-molecular weight perfluorocarbons (PFC) are suitable for therapeutic/biomedical applications involving O2/CO2/NO capture, transport and release (e.g., blood substitutes). However, PFC-in-Water emulsions face challenges related to inadequate emulsion stability, wide-sized droplet distribution and reduced shelf-life, which may be tackled by producing PFC-based nanoemulsions. A novel surfactant system comprising of Tween 80 and (1H,1H,2H,2H-perfluorooctyl)phosphocholine (FC8) was explored to produce monomodal narrow-sized distribution of PFC-in-Water nanoemulsions by ultrasound emulsification. Perfluorodecalin (PFD) was used as the model PFC. Surfactant solutions and emulsions were characterised for surface tension, interfacial tension and emulsion kinetic stability. In comparison to Tween 80, at 4.3 mM, the fluorinated FC8 surfactant-based emulsion droplets were 32 % smaller and six times more stable. Reduction of interfacial tension from 41.5 ± 0.5 mN.m−1 for Tween 80 alone to 20.7 ± 1.8 mN.m−1 for FC8 alone was related to better droplet size distribution of resultant emulsion, with a decrease in Zavg from 255.4 ± 3.9 nm to 172.8 ± 0.72 nm. Equimolar mixture of surfactants resulted in Zavg of 175.8 ± 1.1 nm at a reduced sonication time of 15 min, leading to safer surfactant system with lower energy and operating costs for emulsions preparation.
Usman Taqui Syed; Ana M.A. Dias; Joao Crespo; Carla Brazinha; Hermínio C. de Sousa. Studies on the formation and stability of perfluorodecalin nanoemulsions by ultrasound emulsification using novel surfactant systems. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2021, 616, 126315 .
AMA StyleUsman Taqui Syed, Ana M.A. Dias, Joao Crespo, Carla Brazinha, Hermínio C. de Sousa. Studies on the formation and stability of perfluorodecalin nanoemulsions by ultrasound emulsification using novel surfactant systems. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2021; 616 ():126315.
Chicago/Turabian StyleUsman Taqui Syed; Ana M.A. Dias; Joao Crespo; Carla Brazinha; Hermínio C. de Sousa. 2021. "Studies on the formation and stability of perfluorodecalin nanoemulsions by ultrasound emulsification using novel surfactant systems." Colloids and Surfaces A: Physicochemical and Engineering Aspects 616, no. : 126315.
Algae and seaweeds are used in cookery since the beginnings of human civilization, particularly in several Asian cultures. Phenolic compounds are secondary metabolites produced by aquatic and terrestrial plants for their natural defense against external stimuli, which possess powerful antimicrobial and antioxidant properties that can be very important for the food industry. The main objective of this study was to develop a whey protein concentrate active coating, incorporated with a Fucus vesiculosus extract in order to delay the lipid oxidation of chicken breasts. Ten hydroethanolic extracts from F. vesiculosus were obtained and their antioxidant capacity was evaluated through two antioxidant activity assays: the DPPH radical scavenging activity and β-carotene bleaching assay. The total content in phenolics compounds was also determined by Folin-Ciocalteu method. The chosen extract was the one obtained from the freeze-dried F. vesiculosus using 75% (v/v) ethanol as extraction solvent. The extract was successfully incorporated into a whey protein film and successfully strengthened the thickness, tensile strength, and elastic modulus. The active film also was able to inhibit the chicken breasts lipid oxidation for 25 days of storage.
Mariana Andrade; Cássia Barbosa; Victor Souza; Isabel Coelhoso; João Reboleira; Susana Bernardino; Rui Ganhão; Susana Mendes; Ana Fernando; Fernanda Vilarinho; Ana Sanches Silva; Fernando Ramos. Novel Active Food Packaging Films Based on Whey Protein Incorporated with Seaweed Extract: Development, Characterization, and Application in Fresh Poultry Meat. Coatings 2021, 11, 229 .
AMA StyleMariana Andrade, Cássia Barbosa, Victor Souza, Isabel Coelhoso, João Reboleira, Susana Bernardino, Rui Ganhão, Susana Mendes, Ana Fernando, Fernanda Vilarinho, Ana Sanches Silva, Fernando Ramos. Novel Active Food Packaging Films Based on Whey Protein Incorporated with Seaweed Extract: Development, Characterization, and Application in Fresh Poultry Meat. Coatings. 2021; 11 (2):229.
Chicago/Turabian StyleMariana Andrade; Cássia Barbosa; Victor Souza; Isabel Coelhoso; João Reboleira; Susana Bernardino; Rui Ganhão; Susana Mendes; Ana Fernando; Fernanda Vilarinho; Ana Sanches Silva; Fernando Ramos. 2021. "Novel Active Food Packaging Films Based on Whey Protein Incorporated with Seaweed Extract: Development, Characterization, and Application in Fresh Poultry Meat." Coatings 11, no. 2: 229.
In this study, we report the impact of the magnetic field on protein permeability through magnetic-responsive, block copolymer, nanocomposite membranes with hydrophilic and hydrophobic characters. The hydrophilic nanocomposite membranes were composed of spherical polymeric nanoparticles (NPs) synthesized through polymerization-induced self-assembly (PISA) with iron oxide NPs coated with quaternized poly(2-dimethylamino)ethyl methacrylate. The hydrophobic nanocomposite membranes were prepared via nonsolvent-induced phase separation (NIPS) containing poly (methacrylic acid) and meso-2,3-dimercaptosuccinic acid-coated superparamagnetic nanoparticles (SPNPs). The permeation experiments were carried out using bovine serum albumin (BSA) as the model solute, in the absence of the magnetic field and under permanent and cyclic magnetic field conditions OFF/ON (strategy 1) and ON/OFF (strategy 2). It was observed that the magnetic field led to a lower reduction in the permeate fluxes of magnetic-responsive membranes during BSA permeation, regardless of the magnetic field strategy used, than that obtained in the absence of the magnetic field. Nevertheless, a comparative analysis of the effect caused by the two cyclic magnetic field strategies showed that strategy 2 allowed for a lower reduction of the original permeate fluxes during BSA permeation and higher protein sieving coefficients. Overall, these novel magneto-responsive block copolymer nanocomposite membranes proved to be competent in mitigating biofouling phenomena in bioseparation processes.
Lakshmeesha Upadhyaya; Mona Semsarilar; Damien Quemener; Rodrigo Fernández-Pacheco; Gema Martinez; Isabel Coelhoso; Suzana Nunes; João Crespo; Reyes Mallada; Carla Portugal. Block Copolymer-Based Magnetic Mixed Matrix Membranes—Effect of Magnetic Field on Protein Permeation and Membrane Fouling. Membranes 2021, 11, 105 .
AMA StyleLakshmeesha Upadhyaya, Mona Semsarilar, Damien Quemener, Rodrigo Fernández-Pacheco, Gema Martinez, Isabel Coelhoso, Suzana Nunes, João Crespo, Reyes Mallada, Carla Portugal. Block Copolymer-Based Magnetic Mixed Matrix Membranes—Effect of Magnetic Field on Protein Permeation and Membrane Fouling. Membranes. 2021; 11 (2):105.
Chicago/Turabian StyleLakshmeesha Upadhyaya; Mona Semsarilar; Damien Quemener; Rodrigo Fernández-Pacheco; Gema Martinez; Isabel Coelhoso; Suzana Nunes; João Crespo; Reyes Mallada; Carla Portugal. 2021. "Block Copolymer-Based Magnetic Mixed Matrix Membranes—Effect of Magnetic Field on Protein Permeation and Membrane Fouling." Membranes 11, no. 2: 105.
Usman T. Syed; Inês Leonardo; Ruth Lahoz; Frédéric B. Gaspar; Rosa Huertas; Maria T. B. Crespo; Manuel Arruebo; J. G. Crespo; Victor Sebastian; Carla Brazinha. Microengineered Membranes for Sustainable Production of Hydrophobic Deep Eutectic Solvent-Based Nanoemulsions by Membrane Emulsification for Enhanced Antimicrobial Activity. ACS Sustainable Chemistry & Engineering 2020, 8, 1 .
AMA StyleUsman T. Syed, Inês Leonardo, Ruth Lahoz, Frédéric B. Gaspar, Rosa Huertas, Maria T. B. Crespo, Manuel Arruebo, J. G. Crespo, Victor Sebastian, Carla Brazinha. Microengineered Membranes for Sustainable Production of Hydrophobic Deep Eutectic Solvent-Based Nanoemulsions by Membrane Emulsification for Enhanced Antimicrobial Activity. ACS Sustainable Chemistry & Engineering. 2020; 8 (44):1.
Chicago/Turabian StyleUsman T. Syed; Inês Leonardo; Ruth Lahoz; Frédéric B. Gaspar; Rosa Huertas; Maria T. B. Crespo; Manuel Arruebo; J. G. Crespo; Victor Sebastian; Carla Brazinha. 2020. "Microengineered Membranes for Sustainable Production of Hydrophobic Deep Eutectic Solvent-Based Nanoemulsions by Membrane Emulsification for Enhanced Antimicrobial Activity." ACS Sustainable Chemistry & Engineering 8, no. 44: 1.
Corn fiber, a by-product of the starch industry, is presently incorporated in animal feed. However, it has arabinoxylans as added-value components (besides ferulic acid) that should be valorized. In this work, the raw material, a fraction enriched in arabinoxylans from corn fiber, previously produced by alkaline extraction from corn fiber and pre-concentrated by ultrafiltration, was further purified. The use of ultrafiltration operated in diafiltration mode (dia-ultrafiltration) was evaluated for the purification of the arabinoxylans fraction. The objective was to maximize the removal of the small contaminants from the fraction and to maximize the permeability and/or the permeate flux, by selecting the relevant operating conditions involved in this process. The removal of contaminants (%) was estimated when their apparent rejection stabilized. Edible films were produced, from the resultant purified arabinoxylans fraction, using glycerol as plasticizer (30% dry basis). Additionally, films with the incorporation of ferulic acid were developed, in order to obtain barriers with antioxidant activity. The films were characterized in terms of mechanical properties, antioxidant activity and permeability to water vapor. The films prepared presented a good potential to be used as packaging for food products with low water content.
Maria Serra; Verónica Weng; Isabel M. Coelhoso; Vitor D. Alves; Carla Brazinha. Purification of Arabinoxylans from Corn Fiber and Preparation of Bioactive Films for Food Packaging. Membranes 2020, 10, 95 .
AMA StyleMaria Serra, Verónica Weng, Isabel M. Coelhoso, Vitor D. Alves, Carla Brazinha. Purification of Arabinoxylans from Corn Fiber and Preparation of Bioactive Films for Food Packaging. Membranes. 2020; 10 (5):95.
Chicago/Turabian StyleMaria Serra; Verónica Weng; Isabel M. Coelhoso; Vitor D. Alves; Carla Brazinha. 2020. "Purification of Arabinoxylans from Corn Fiber and Preparation of Bioactive Films for Food Packaging." Membranes 10, no. 5: 95.
Nowadays, the reutilization of anaesthetic gases is accomplished by capturing carbon dioxide with soda lime, a solid adsorbent mostly composed by calcium and sodium hydroxide. To overcome the issues regarding the use of soda lime, this work proposes an alternative process to remove carbon dioxide through the use of a membrane contactor combined with a biocompatible ionic liquid (IL), cholinium lysinate, with high absorption capacity (5.9 molCO2/kgIL). The carbon dioxide removal rate and IL solution regeneration, were assessed, varying the feed gas composition, relative humidity and ionic liquid flow rate conditions. Overall mass transfer coefficients and separation factors were determined. From the results obtained, the proposed system is feasible to remove carbon dioxide from anaesthetic gas circuits. Moreover, the system working operation time obtained was 63 hours, which in a mass basis comparison with soda lime (current technology), is 3 to 5 times higher.
Charles Martins; L.A. Neves; Ricardo Chagas; Luísa Ferreira; C.A.M. Afonso; J.G. Crespo; I.M. Coelhoso. CO2 removal from anaesthesia circuits using gas-ionic liquid membrane contactors. Separation and Purification Technology 2020, 250, 116983 .
AMA StyleCharles Martins, L.A. Neves, Ricardo Chagas, Luísa Ferreira, C.A.M. Afonso, J.G. Crespo, I.M. Coelhoso. CO2 removal from anaesthesia circuits using gas-ionic liquid membrane contactors. Separation and Purification Technology. 2020; 250 ():116983.
Chicago/Turabian StyleCharles Martins; L.A. Neves; Ricardo Chagas; Luísa Ferreira; C.A.M. Afonso; J.G. Crespo; I.M. Coelhoso. 2020. "CO2 removal from anaesthesia circuits using gas-ionic liquid membrane contactors." Separation and Purification Technology 250, no. : 116983.
Sofia C. Fraga; Anna Kujawska; Wojciech Kujawski; Carla Brazinha; João G. Crespo. Corrigendum to ‘Transport of dilute organics through dense membranes: Assessing impact on membrane-solute interactions’ [J. Membr. Sci. 523 (2017) 346–354]. Journal of Membrane Science 2020, 603, 117424 .
AMA StyleSofia C. Fraga, Anna Kujawska, Wojciech Kujawski, Carla Brazinha, João G. Crespo. Corrigendum to ‘Transport of dilute organics through dense membranes: Assessing impact on membrane-solute interactions’ [J. Membr. Sci. 523 (2017) 346–354]. Journal of Membrane Science. 2020; 603 ():117424.
Chicago/Turabian StyleSofia C. Fraga; Anna Kujawska; Wojciech Kujawski; Carla Brazinha; João G. Crespo. 2020. "Corrigendum to ‘Transport of dilute organics through dense membranes: Assessing impact on membrane-solute interactions’ [J. Membr. Sci. 523 (2017) 346–354]." Journal of Membrane Science 603, no. : 117424.
Codfish blood and sardine cooking wastewaters were processed using membrane ultrafiltration that allowed for the preparation of bioactive peptides enriched fractions. The raw materials and corresponding permeates were characterized chemically and in terms of biological properties. The fractionation process was evaluated by analyzing the selective permeation of small peptides (10 kDa was achieved for both raw materials with the studied membranes. Also, low values of rejection of peptides <1 kDa were accomplished, namely 2% with UP010 from codfish blood and 23% when operated at minimum pressure (1.0 bar) with GH from sardine wastewaters. The peptide fractions from codfish blood with MW and UP010 exhibited the highest ABTS+ and ORAC values. Peptide fractions from sardine wastewaters with GH demonstrated no improvement in antioxidant activity compared to sardine wastewaters. The antimicrobial results showed that the peptide fractions from codfish blood with UP010 and from sardine with GH at 1.0 bar were capable of inhibiting Escherichia coli growth.
Soudabeh Ghalamara; Sara Silva; Carla Brazinha; Manuela Pintado. Valorization of Fish by-Products: Purification of Bioactive Peptides from Codfish Blood and Sardine Cooking Wastewaters by Membrane Processing. Membranes 2020, 10, 44 .
AMA StyleSoudabeh Ghalamara, Sara Silva, Carla Brazinha, Manuela Pintado. Valorization of Fish by-Products: Purification of Bioactive Peptides from Codfish Blood and Sardine Cooking Wastewaters by Membrane Processing. Membranes. 2020; 10 (3):44.
Chicago/Turabian StyleSoudabeh Ghalamara; Sara Silva; Carla Brazinha; Manuela Pintado. 2020. "Valorization of Fish by-Products: Purification of Bioactive Peptides from Codfish Blood and Sardine Cooking Wastewaters by Membrane Processing." Membranes 10, no. 3: 44.
In this work, the influence of surface topography on protein crystallization over Nafion® is investigated. Two types of Nafion® based membranes were modified by soft lithographic techniques in order to create different topographies at the micro and nano scale and subsequently tested. From the analysis of the induction time, nucleation and crystal growth rate of Trypsin from Bovine Pancreas, all the patterned Nafion® based membranes show an enhanced nucleation and crystal growth. To provide additional insight to the experimental observations, the wettability properties of the prepared samples and the ratio of the Gibbs free energy of heterogeneous nucleation to homogeneous nucleation were evaluated. The crystallization outcome results from the combined effect of both, the structural and chemical properties of the nucleant Nafion® surface.
M. Polino; C. A. M. Portugal; H. Le The; Roald M. Tiggelaar; J. Eijkel; J. G. Crespo; I. M. Coelhoso; M. P. Pina; R. Mallada. Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques. Crystal Growth & Design 2020, 20, 2174 -2186.
AMA StyleM. Polino, C. A. M. Portugal, H. Le The, Roald M. Tiggelaar, J. Eijkel, J. G. Crespo, I. M. Coelhoso, M. P. Pina, R. Mallada. Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques. Crystal Growth & Design. 2020; 20 (4):2174-2186.
Chicago/Turabian StyleM. Polino; C. A. M. Portugal; H. Le The; Roald M. Tiggelaar; J. Eijkel; J. G. Crespo; I. M. Coelhoso; M. P. Pina; R. Mallada. 2020. "Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques." Crystal Growth & Design 20, no. 4: 2174-2186.
Chitosan-based composites play an important role in food packaging applications and can be used either as films or as edible coatings. Due to their high costs and lower performance (i.e., lower barrier against water vapor, thermal, and mechanical properties) when compared to the traditional petroleum-based plastics, the use of such biopolymers in large-scale is still limited. Several approaches of chitosan composites in the packaging industry are emerging to overcome some of the disadvantages of pristine polymers. Thus, this work intends to present the current trends and the future challenges towards production and application of chitosan composites in the food packaging industry.
Victor G. L. Souza; João R. A. Pires; Carolina Rodrigues; Isabel M. Coelhoso; Ana Luísa Fernando. Chitosan Composites in Packaging Industry—Current Trends and Future Challenges. Polymers 2020, 12, 417 .
AMA StyleVictor G. L. Souza, João R. A. Pires, Carolina Rodrigues, Isabel M. Coelhoso, Ana Luísa Fernando. Chitosan Composites in Packaging Industry—Current Trends and Future Challenges. Polymers. 2020; 12 (2):417.
Chicago/Turabian StyleVictor G. L. Souza; João R. A. Pires; Carolina Rodrigues; Isabel M. Coelhoso; Ana Luísa Fernando. 2020. "Chitosan Composites in Packaging Industry—Current Trends and Future Challenges." Polymers 12, no. 2: 417.
The advances on the development of novel materials capable to enhance the shelf life of food products may contribute to reduce the current worldwide food waste problem. Zinc oxide nanoparticles (ZnO NPs) are considered GRAS (Generally Recognized as Safe) by the Food and Drug Administration (FDA) and due to their good antimicrobial properties are suitable to be applied as active compounds in food packaging. ZnO NPs were synthesized to be tested in active bionanocomposites through an eco-friendlier route using apple peel wastes. This work aimed to develop bionanocomposites based on chitosan and incorporated with ZnO NPs to characterize its bioactivity via in vitro and in situ studies, using fresh poultry meat as the food matrix. Overall, bio-based biodegradable films presented good antimicrobial activity, being the intrinsic antimicrobial properties of chitosan enhanced by the ZnO NPs added on the system. When used as primary packaging of the meat, the samples protected with the films presented a decrease on the deterioration speed, which was represented by the preservation of the initial reddish color of the meat and reduction on the oxidation process and microbiological growth. The nanoparticles enhanced especially the antioxidant properties of the films and proved to be potential food preservatives agents to be used in active food packaging.
Victor Gomes Lauriano Souza; Carolina Rodrigues; Sara Valente; Catarina Pimenta; João Ricardo Afonso Pires; Marta M. Alves; Catarina F. Santos; Isabel M. Coelhoso; Ana Luísa Fernando. Eco-Friendly ZnO/Chitosan Bionanocomposites Films for Packaging of Fresh Poultry Meat. Coatings 2020, 10, 110 .
AMA StyleVictor Gomes Lauriano Souza, Carolina Rodrigues, Sara Valente, Catarina Pimenta, João Ricardo Afonso Pires, Marta M. Alves, Catarina F. Santos, Isabel M. Coelhoso, Ana Luísa Fernando. Eco-Friendly ZnO/Chitosan Bionanocomposites Films for Packaging of Fresh Poultry Meat. Coatings. 2020; 10 (2):110.
Chicago/Turabian StyleVictor Gomes Lauriano Souza; Carolina Rodrigues; Sara Valente; Catarina Pimenta; João Ricardo Afonso Pires; Marta M. Alves; Catarina F. Santos; Isabel M. Coelhoso; Ana Luísa Fernando. 2020. "Eco-Friendly ZnO/Chitosan Bionanocomposites Films for Packaging of Fresh Poultry Meat." Coatings 10, no. 2: 110.
A novel cellulose-based cross-linked polymer, dicarboxymethyl cellulose (DCMC), has been synthesized and used for methylene blue (MB) removal. Inductively coupled plasma atomic emission spectrometry (ICP-AES), Fourier-transform infrared spectroscopy (FTIR), nitrogen porosimetry, and optical microscopy were employed to characterize the structure of the cellulose-based adsorbent. The number of carboxylate groups per gram of polymer (CG) was calculated with sodium content determined by ICP-AES. Systematic equilibrium and kinetic adsorption studies were performed to assess the polymer suitability for dye removal. The effect of pH on its adsorption capacity was also studied and the equilibrium adsorption data was analyzed using Langmuir, Freundlich, and Sips isotherms. At pH = 3, the adsorption isotherms followed the Langmuir model with a maximum adsorption capacity of 887.6 mg/g. At pH = 6.4, the adsorption isotherms produced S-shape curves and were best fitted with the Sips model. The maximum MB uptake increased to 1354.6 mg/g. Pseudo first-order and second-order models were used to fit the kinetic data. A pseudo second-order kinetic model provided the best correlation for the adsorption of MB onto DCMC. Adsorption coupled with membrane filtration achieved 95% methylene blue removal and DCMC can be successfully regenerated and reused in consecutive experiments.
Diana Gago; Ricardo Chagas; Luísa M. Ferreira; Svetlozar Velizarov; Isabel Coelhoso. A Novel Cellulose-Based Polymer for Efficient Removal of Methylene Blue. Membranes 2020, 10, 13 .
AMA StyleDiana Gago, Ricardo Chagas, Luísa M. Ferreira, Svetlozar Velizarov, Isabel Coelhoso. A Novel Cellulose-Based Polymer for Efficient Removal of Methylene Blue. Membranes. 2020; 10 (1):13.
Chicago/Turabian StyleDiana Gago; Ricardo Chagas; Luísa M. Ferreira; Svetlozar Velizarov; Isabel Coelhoso. 2020. "A Novel Cellulose-Based Polymer for Efficient Removal of Methylene Blue." Membranes 10, no. 1: 13.