Saad Fathallah Elalem

Prof. Saad Fathallah Elalem

orcid

Research Focus & Metrics

Academic Career Length

3Years (2024-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 January 2024 in Open Life Sciences
Verified
Journal Article
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Helmy Abdelhakim AamerHelmy Abdelhakim Aamer
Helmy Abdelhakim Aamer
SESaad Fathallah Elalem
Saad Fathallah Elalem
AAAbdulaziz Al-Askar
Abdulaziz Al-Askar
OSOmaima A. Sharaf
Omaima A. Sharaf
MGMahmoud A. Gaber
Mahmoud A. Gaber
Ahmed AbdelkhalekAhmed Abdelkhalek
Ahmed Abdelkhalek
Methanolic extract from Salsola imbricata was investigated for its phytochemical content, antioxidant, and antimicrobial properties against phytopathogenic fungi and bacteria. Phytochemical analysis revealed the presence of saponin, tannins, and alkaloids with 1.25%, 18.8 mg catechin/g of extract, and 9.12%, respectively. Total flavonoid content was 20.8 mg quercetin equivalent/g while total phenolic content was 202 mg gallic acid equivalent/g. Antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay resulted in an IC50 value of 48.61 µg/mL, while the phosphomolybdenum method yielded a value of 215.43 mg ascorbic acid equivalent/g of extract. The highest phenolic acids detected in the extract were gallic acid (712.97 µg/g), syringic acid (742.7 µg/g), and caffeic acid (474.70 µg/g) according to high-performance liquid chromatography analysis. Palmitic acid (28.38%) dominated the fatty acids identified by gas chromatography–mass spectrometry, while stigmasterol (8.34%) was the most abundant steroid. At a concentration of 3 mg/mL, the extract showed strong antibacterial activity against Pectobacterium carotovorum (10.50 mm), Ralstonia solanacearum (9.93 mm), and Pectobacterium atrosepticum (8.37 mm). Additionally, the extract significantly suppressed fungal growth of Rhizoctonia solani (38.22%) and Fusarium oxysporum (33.56%) but showed lower activity toward Botrytis cinerea (13.33%) at 5 mg/mL. In conclusion, S. imbricata extract exhibited promising antioxidant and antimicrobial properties, making it a potential candidate for further exploration in agricultural applications.

1 September 2024 in Polish Journal of Chemical Technology
Verified
Journal Article
Open access
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Ahmed AbdelkhalekAhmed Abdelkhalek
Ahmed Abdelkhalek
EAEman Abdelaziz Abdelwahab
Eman Abdelaziz Abdelwahab
SESaad Fathallah Elalem
Saad Fathallah Elalem
AAAbdulaziz Al-Askar
Abdulaziz Al-Askar
Przemysław Łukasz KowalczewskiPrzemysław Łukasz Kowalczewski
Przemysław Łukasz Kowalczewski
Said I. BehirySaid I. Behiry
Said I. Behiry
The present study appraised the inhibitory role of ethanol (PDEE) and ethyl acetate (PDEAE) extracts of Phoenix dactylifera L. against three molecularly identified fungi: Fusarium oxysporum , Botrytis cinerea , and Rhizoctonia solani . HPLC analysis revealed that gallic acid was the major phenolic compound in both extracts: (PDEE: 1721.90 μg/g) and (PDEAE: 101.53 μg/g). The major flavonoids in PDEE are rutin, kaempferol, and quercetin, whereas PDEAE contains kaempferol, naringenin, and quercetin. The GC-MS showed 11-octadecenoic acid methyl ester (26.25%) is the highest compound in PDEE, while diisooctyl phthalate (18.82%) is the most important compound in PDEAE. At 50 μg/mL, the inhibition percentage of PDEAE initiated the highest growth inhibition of F. oxysporum (49.63%) and R. solani (71.43%). Meanwhile, PDEE at 200 μg/mL initiated an inhibition value of 77.78% for B. cinerea . As a result, PDEAE is considered more effective than PDEE in controlling the growth of selected isolates.