A sustainable use of locally available wastes from agriculture as supplementary cementitious materials (SCMs) is an alternative solution for the prevention of excessive raw material usage, reduction of CO₂ emission and cost-effective concrete production. This paper studies the reactivity of non-traditional waste SCMs: Wheat straw ash (WSA), mixture of wheat and soybean straw ash (WSSA) and soybean straw ash (SSA), which are abundant as agricultural by-products in Serbia. The chemical evaluation using XRF technique, thermal analysis (TGA/DSC), XRD and FTIR methods were performed along with physical properties tests to investigate the feasibility of utilizing biomass ashes as cement substitutes. The obtained results demonstrate a high pozzolanic activity of WSA, which is attributed to a high reactive silica content of the ash and its satisfactory level of fineness. A wider hump in XRD pattern of WSA compared to WSSA and SSA confirmed that it abounds in amorphous (reactive) phase. The insufficient activity index of soybean-based biomass ashes, characterized with a low silica content, was improved by additional grinding and/or blending with amorphous silica-rich material. This points out the mechanical activation, i.e., grinding procedure, and chemical activation, i.e., modification of the chemical composition, as techniques efficient at producing pozzolanic materials from biomass wastes. Tested biomass ashes are characterized with negligible leaching values of heavy metals, thereby satisfying eco-friendly principles of SCM utilization. The application of biomass ashes as SCMs leads to substantial cost savings, as well as benefits to the environment, such as lower consumption of cement, reduction of CO₂ emissions during the production of cement and sustainable waste management.

As the cement industry is responsible for 7% of the global CO₂ emissions, locally and abundantly available materials are vastly valorized, and their use is assuming a significant role in this domain. Over the last few decades, significant research in the development of supplementary cementitious materials (SCMs) derived from industrial wastes, such as fly ash (FA), has been conducted. However, facing environmental pressures, coal power plants are closing across the planet. Hence there is an urgent need to identify sustainable SCMs that can replace FA in the concrete industry. Furthermore, the usage of FA in cement-based composites does not often produce satisfactory results from the aspect of certain properties, such as freeze–thaw durability. Therefore, the application of natural zeolites (NZs) for these purposes has emerged as an area of interest in the civil engineering practice. This paper presents the results of experimental research regarding the influence of NZ, as a mineral admixture, on the basic physical and mechanical properties of cement mortars, with a focus on frost resistance and drying shrinkage. The amount of NZ was varied from 10 to 30% in relation to cement mass. The findings indicate that NZ positively influences the drying shrinkage reduction regardless of the replacement level, while the best results concerning frost resistance can be achieved in cement blends with 10% NZ.

Using a Taguchi experimental design, this research focuses on utilizing indigenous bacteria from the Danube River to enhance the self-healing capabilities and structural integrity of cementitious materials. Bacillus licheniformis and Bacillus muralis were used as individual bacterium or in co-culture, with a concentration of 8 logs CFU, while the humidity variation involved testing wet and wet–dry conditions. Additionally, artificial neural network (ANN) modeling of the compressive strength of cement samples results in improvements in compressive strength, particularly under wet–dry conditions. By inducing targeted bacterial activity, the formation of calcium carbonate precipitates was initiated, which effectively sealed formed cracks, thus restoring and even enhancing the material’s strength. In addition to short-term improvements, this study also evaluates long-term improvements, with compressive strength measured over periods extending to 180 days. The results demonstrate sustained self-healing capabilities and strength improvements under varied environmental conditions, emphasizing the potential for long-term application in real-world infrastructure. This study also explores the role of environmental conditions, such as wet and wet–dry cycles, in optimizing the self-healing process, revealing that cyclic exposure conditions further improve the efficiency of strength recovery. The findings suggest that autochthonous bacterial co-cultures can be a viable solution for enhancing the durability and lifespan of concrete structures. This research provides a foundation for further exploration into bio-based self-healing mechanisms and their practical applications in the concrete industry.

This paper presents an analysis of the stabilization of different soil types using a hydraulic binder. A study was carried out on soils that can be classified into two groups: cohesive and non-cohesive soils. Clay soils of medium and low plasticity according to the USCS classification were used as cohesive materials, while the sandy material containing dust was considered as non-cohesive material. Samples were taken from fifteen locations in Vojvodina province, Serbia. A hydraulic binder was used as a binder based on cement and lime. The amounts of the binder were estimated at 3, 5, 7, and 9%. In order to determine the basic physical and mechanical characteristics of the specimens, the following tests were performed: unconfined compressive strength after 7 and 28 days, indirect tensile strength after 7 and 28 days, as well as the California Bearing Ratio. Based on the obtained results, it can be concluded that increasing the amount of binder results in an increase in the subgrade load-bearing capacity. However, it should be emphasized that the subgrade containing non-cohesive material had a lower growth in the load-bearing capacity than those with the cohesive material.

This study investigates the potential of bioaugmentation with Bacillus species to enhance wastewater treatment and develop a bacterial–CaCO₃ system for self-healing cement applications. Utilizing microbiologically induced calcium carbonate precipitation (MICP), this study evaluates the dual functionality of Bacillus licheniformis and B. muralis strains. For wastewater treatment, the bioaugmentation process achieved significant pollutant reductions, including a 99.52% decrease in biochemical oxygen demand (BOD₅), a 92.13% reduction in chemical oxygen demand (COD), and a substantial removal of heavy metals and nutrients. This process also produced high concentrations of CaCO₃ precipitate enriched with viable bacterial cells, demonstrating an eco-friendly approach to improving water quality. For self-healing cement applications, bioaugmented CaCO₃ crystals were coated with nutrient and sodium silicate layers to form a bacterial–CaCO₃ coupled system. This system demonstrated a 92% recovery in compressive strength after 180 days, highlighting its ability to autonomously repair microcracks in cement-based materials. The layered encapsulation strategy ensured bacterial viability and a controlled activation mechanism, offering a scalable and sustainable solution for infrastructure resilience. This dual-function approach addresses critical environmental and construction challenges by linking efficient wastewater treatment with innovative self-healing material development, contributing to global sustainability and circular economy goals.

The integration of bacterial biotechnology into construction and geotechnical practices is redefining approaches to material sustainability, infrastructure longevity, and environmental resilience. Over the past two decades, research activity in construction biotechnology has expanded rapidly, with more than 350 publications between 2000 and 2024 and a five-fold increase in annual output since 2020. Beyond bibliometric growth, technical studies have demonstrated the remarkable performance of bacterial systems: for example, microbial-induced calcium carbonate precipitation (MICP) can increase the compressive strength of treated soils by 60–70% and reduce permeability by more than 90% in field-scale trials. In concrete applications, bacterial self-healing has been shown to seal cracks up to 0.8 mm wide and improve water tightness by 70–90%. Similarly, biofilm-mediated corrosion barriers can extend the durability of reinforced steel by significantly reducing chloride ingress, while bacterial biopolymers such as xanthan gum and curdlan enhance soil cohesion and water retention in eco-grouting and erosion control. The novelty of this review lies in its interdisciplinary scope, integrating microbiological mechanisms, materials science, and engineering practice to highlight how bacterial processes can transition from laboratory models to real-world applications. By combining quantitative evidence with critical assessment of scalability, biosafety, and regulatory challenges, this paper provides a comprehensive framework that positions construction biotechnology as a transformative pathway towards low-carbon, adaptive, and resilient infrastructure systems.