The electrode based on cerium oxide (CeO₂) nanorods embedded in nickel hydroxide (Ni(OH)₂) matrix were prepared and used for detecting glucose non-enzymatically. The materials were characterized by X-ray diffraction, transmission electron microscopy (TEM), and so on. The results indicate that the response of CeO₂/Ni(OH)₂ nanocomposite are significantly improved due to the synergetic effect between CeO₂ and Ni(OH)₂. The optimum CeO₂/Ni(OH)₂ nanocomposite electrode exhibits a detection range from 2 μM to 6.62 mM, a sensitivity of 594 μA mM⁻¹ cm⁻², an estimated detection limit of 1.13 μM, and a response time less than 5 s. In addition, this biosensor also shows good selectivity, long term stability, and accurate measurement in juice on sale.