This study explores the relationship between nutrient concentration (NC) and epidermal thickness (d) of the leaves of hydroponically grown Brassica rapa and its attenuation coefficients (m) using portable Time-Domain Optical Coherence Tomography (TD-OCT), which is a non-invasive imaging technique that uses low-coherence interferometry to generate axial scans of plants’ leaves by measuring the time delay and intensity of backscattered light. The portable TD-OCT system in this study has an axial and lateral resolution of 7 m and 3 m, respectively, a scanning depth of 12 mm, and a 1310 nm Super Luminescent Diode (SLD). Several studies suggest that the differences in d and m are related to nutritional, physiological, and anatomical status. The study used the Kratky method, a simple non-circulating hydroponic system, to cultivate Brassica rapa with varying NC (25%, 50%, 75%, 100% (control), and 125%). Each treatment group used two plants. The TD-OCT sample probe was placed on a fixed holder and was oriented vertically so that light was directed downward onto the leaf’s surface to obtain the depth profile (A-scan). The distance between the probe and the leaf was adjusted to obtain the optimum interference signal. Five averaged A-scans were obtained per leaf on the 7th, 18th, and 21st days post nutrient exposure. The logarithm of the averaged A-scan is linearly fitted to extract m. The results showed a positive correlation between NC and m, which suggests that plants produce more chlorophyll and develop denser cells and increase m. There was no correlation obtained between NC and d. The study demonstrates the potential of TD-OCT as a non-destructive tool for assessing plant health and monitoring growth dynamics in hydroponic systems and m as a sensitive indicator of plant health as compared to d. The continued exploration of TD-OCT applications in agriculture can contribute to improving crop management strategies and promoting sustainable food production practices.