Endoscopes are flexible medical instruments that require a high-level disinfection protocol. This study aimed to develop a fluid-dynamic system with ozonated water to obtain high-level disinfection. A colonoscope replica with an internal flexible polytetrafluoroethylene (PTFE) tube was made. The set was then contaminated, washed, and disinfected with ozonated water. The replica was placed in a tank with a fluid-dynamic circuit. The endoscope was submerged into the tank and ozonated water was pumped through the working channel. Quantitative microbiological tests were carried out between the washing stage and disinfection in the fluid-dynamic system with ozonated water. The results indicated that the ozone concentration curve in the water from the 27- liter main tank reached the value of 0.95 mg/L after 20 minutes. No bacterial growth was detected after the replica remained in the hydrodynamic system with ozonated water for 15 minutes. The results showed that the fluid-dynamic system using ozonated water is promising as it enables high-level disinfection of the colonoscope.

Resumo Objetivo Analisar as evidências científicas com relação à eficácia do uso do ozônio para desinfecção de superfícies a partir de uma revisão integrativa de literatura. Métodos Realizou-se busca nos bancos de dados eletrônicos: SciELO, MEDLINE, LILACS, PubMed, Sciense Direct. Foram analisados onze artigos, publicados no período de janeiro de 2010 a agosto de 2021. Todos empregaram o modelo de investigação experimental laboratorial e alcançaram diferentes níveis de desinfecção pelo O3, no entanto, com variadas superfícies e produtos testados, além [...] Resumo Objetivo Analisar as evidências científicas com relação à eficácia do uso do ozônio para desinfecção de superfícies a partir de uma revisão integrativa de literatura. Métodos Realizou-se busca nos bancos de dados eletrônicos: SciELO, MEDLINE, LILACS, PubMed, Sciense Direct. Foram analisados onze artigos, publicados no período de janeiro de 2010 a agosto de 2021. Todos empregaram o modelo de investigação experimental laboratorial e alcançaram diferentes níveis de desinfecção pelo O3, no entanto, com variadas superfícies e produtos testados, além

The aim of this study was to evaluate whether a system involving ozonated water and ultrasound causes de-epithelization of the human amniotic membrane (HAM). The experiment protocol was carried out in four stages. Stage I was carried out to determine the duration of the experiment. Stage II comprised the first experiment, involving four groups of samples studied in triplicate: control/natural (IN), processed with ultrasound in a liquid medium (US), processed with ozonated water (O3), and processed with ozonated water combined with ultrasound (US_O3). Stage III was performed to confirm the results, following the same steps present in Stage II. Stage IV involved the use of oxygen to confirm the hypothesis. Histological analysis was carried out to verify whether the effects of O₂ were similar to those of O₃. The system was activated, and ozonation was carried out for 10 min, as in the previous experiment, reaching a concentration level of 3.0 mg/L. The samples were submerged and positioned in the reservoir and processed separately for 55 min. The biochemical properties were assessed using Fourier transform infrared spectroscopy, and the morphology was examined using histology and scanning electron microscopy. The spectra of the samples exhibited similarities; however, subtle changes were highlighted, such as smooth band shifts and intensity changes. The morphology indicated that ultrasound achieved more efficient HAM de-epithelialization compared to ultrasound combined with ozonated water and ozonated water alone. One plausible hypothesis for this observation is that cavitation represents the primary mechanism responsible for de-epithelialization. When ultrasound is combined with ozone, the bubbles generated by ozone gas reduce the cavitation effect. This study is pioneering as it demonstrates an ultrasound system capable of the efficient de-epithelialization of the HAM.