The hybridoma cell screening method is usually done manually by human eyes during the production process for monoclonal antibody drugs. This traditional screening method has certain limitations, such as low efficiency and subjectivity bias. Furthermore, most of the existing deep learning-based image segmentation methods have certain drawbacks, due to different shapes of hybridoma cells and uneven location distribution. In this paper, we propose a deep hybridoma cell image segmentation method based on residual and attention U-Net (RA-UNet). Firstly, the feature maps of the five modules in the network encoder are used for multi-scale feature fusion in a feature pyramid form and then spliced into the network decoder to enrich the semantic level of the feature maps in the decoder. Secondly, a dual attention mechanism module based on global and channel attention mechanisms is presented. The global attention mechanism (non-local neural network) is connected to the network decoder to expand the receptive field of the feature map and bring more rich information to the network. Then, the channel attention mechanism SENet (the squeeze-and-excitation network) is connected to the non-local attention mechanism. Consequently, the important features are enhanced by the learning of the feature channel weights, and the secondary features are suppressed, hence improving the cell segmentation performance and accuracy. Finally, the focal loss function is used to guide the network to learn the hard-to-classify cell categories. Furthermore, we evaluate the performance of the proposed RA-UNet method on a newly established hybridoma cell image dataset. Experimental results show that the proposed method has good reliability and improves the efficiency of hybridoma cell segmentation compared with state-of-the-art networks such as FCN, UNet, and UNet++. The results show that the proposed RA-UNet model has improvements of 0.8937%, 0.9926%, 0.9512%, and 0.9007% in terms of the dice coefficients, PA, MPA, and MIoU, respectively.

This paper proposes a screen-shooting resilient watermarking scheme via learned invariant keypoints and QT; that is, if the watermarked image is displayed on the screen and captured by a camera, the watermark can be still extracted from the photo. A screen-shooting resilient watermarking algorithm should meet the following two basic requirements: robust keypoints and a robust watermark algorithm. In our case, we embedded watermarks by combining the feature region filtering model to SuperPoint (FRFS) neural networks, quaternion discrete Fourier transform (QDFT), and tensor decomposition (TD). First we applied FRFS to locate the embedding feature regions which are decided by the keypoints that survive screen-shooting. Second, we structured watermark embedding regions centered at keypoints. Third, the watermarks were embedded by the QDFT and TD (QT) algorithm, which is robust for capturing process attacks. In a partial shooting scenario, the watermark is repeatedly embedded into different regions in an image to enhance robustness. Finally, we extracted the watermarks from at least one region at the extraction stage. The experimental results showed that the proposed scheme is very robust for camera shooting (including partial shooting) different shooting scenarios, and special attacks. Moreover, the efficient mechanism of screen-shooting resilient watermarking could have propietary protection and leak tracing applications.

Robust reversible watermarking in an encrypted domain is a technique that preserves privacy and protects copyright for multimedia transmission in the cloud. In general, most models of buildings and medical organs are constructed by three-dimensional (3D) models. A 3D model shared through the internet can be easily modified by an unauthorized user, and in order to protect the security of 3D models, a robust reversible 3D models watermarking method based on homomorphic encryption is necessary. In the proposed method, a 3D model is divided into non-overlapping patches, and the vertex in each patch is encrypted by using the Paillier cryptosystem. On the cloud side, in order to utilize addition and multiplication homomorphism of the Paillier cryptosystem, three direction values of each patch are computed for constructing the corresponding histogram, which is shifted to embed watermark. For obtaining watermarking robustness, the robust interval is designed in the process of histogram shifting. The watermark can be extracted from the symmetrical direction histogram, and the original encrypted model can be restored by histogram shifting. Moreover, the process of watermark embedding and extraction are symmetric. Experimental results show that compared with the existing watermarking methods in encrypted 3D models, the quality of the decrypted model is improved. Moreover, the proposed method is robust to common attacks, such as translation, scaling, and Gaussian noise.

With high performances of image capturing tools, image information can be easily obtained by screenshots that make image copyright protection a challenging task. The existing screen-shooting watermarking algorithms suffer from a huge running time, in addition to their low robustness against different screenshot attacks, such as different distances and capturing angles of the screenshots. In this paper, a fast and robust high-capacity flexible watermarking algorithm for screenshot images is proposed. Firstly, Oriented FAST and Rotated BRIEF (ORB) feature points are extracted from the input image. Secondly, the feature points are then sorted in a descending order according to their response values. Then, the first five non-overlapping feature points are selected for the embedding by using Hamming window-based filtering method. Furthermore, we exploit the multi-resolution property of Discrete Wavelet Transform (DWT) and energy compaction property of Singular Value Decomposition (SVD) to embed the watermark. Therefore, the classical DWT combined with Singular Value Decomposition (SVD) are adopted to improve the robustness and capacity of the proposed watermarking algorithm. At the extraction side, the sum of the response values for the three RGB channels of the color-ripped image is calculated to improve the feature point localization accuracy. Experimental results show that the proposed screen-shooting watermarking algorithm improves running speed while ensuring the robustness. Furthermore, it has less time complexity and high robustness compared with the state-of-the-art watermarking algorithms against different screenshot attacks.

To protect the copyright of the color image, a color image watermarking scheme based on quaternion discrete Fourier transform (QDFT) and tensor decomposition (TD) is presented. Specifically, the cover image is partitioned into non-overlapping blocks, and then QDFT is performed on each image block. Then, the three imaginary frequency components of QDFT are used to construct a third-order tensor. The third-order tensor is decomposed by Tucker decomposition and generates a core tensor. Finally, an improved odd–even quantization technique is employed to embed a watermark in the core tensor. Moreover, pseudo-Zernike moments and multiple output least squares support vector regression (MLS–SVR) network model are used for geometric distortion correction in the watermark extraction stage. The scheme utilizes the inherent correlations among the three RGB channels of a color image, and spreads the watermark into the three channels. The experimental results indicate that the proposed scheme has better fidelity and stronger robustness for common image-processing and geometric attacks, can effectively resist each color channel exchange attack. Compared with the existing schemes, the presented scheme achieves better performance.