This page has only limited features, please log in for full access.

Unclaimed
Eric Lainé
USC INRAE 1328, Laboratoire LBLGC, Antenne Scientifique Universitaire de Chartres, University of Orleans, 28000 Chartres, France

Basic Info

Basic Info is private.

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 02 February 2021 in Molecules
Reads 0
Downloads 0

Lignans, phenolic plant secondary metabolites, are derived from the phenylpropanoid biosynthetic pathway. Although, being investigated for their health benefits in terms of antioxidant, antitumor, anti-inflammatory and antiviral properties, the role of these molecules in plants remains incompletely elucidated; a potential role in stress response mechanisms has been, however, proposed. In this study, a non-targeted metabolomic analysis of the roots, stems, and leaves of wild-type and PLR1-RNAi transgenic flax, devoid of (+) secoisolariciresinol diglucoside ((+) SDG)—the main flaxseed lignan, was performed using 1H-NMR and LC-MS, in order to obtain further insight into the involvement of lignan in the response of plant to osmotic stress. Results showed that wild-type and lignan-deficient flax plants have different metabolic responses after being exposed to osmotic stress conditions, but they both showed the capacity to induce an adaptive response to osmotic stress. These findings suggest the indirect involvement of lignans in osmotic stress response.

ACS Style

Kamar Hamade; Ophélie Fliniaux; Jean-Xavier Fontaine; Roland Molinié; Elvis Otogo Nnang; Solène Bassard; Stéphanie Guénin; Laurent Gutierrez; Eric Lainé; Christophe Hano; Serge Pilard; Akram Hijazi; Assem El Kak; François Mesnard. NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax. Molecules 2021, 26, 767 .

AMA Style

Kamar Hamade, Ophélie Fliniaux, Jean-Xavier Fontaine, Roland Molinié, Elvis Otogo Nnang, Solène Bassard, Stéphanie Guénin, Laurent Gutierrez, Eric Lainé, Christophe Hano, Serge Pilard, Akram Hijazi, Assem El Kak, François Mesnard. NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax. Molecules. 2021; 26 (3):767.

Chicago/Turabian Style

Kamar Hamade; Ophélie Fliniaux; Jean-Xavier Fontaine; Roland Molinié; Elvis Otogo Nnang; Solène Bassard; Stéphanie Guénin; Laurent Gutierrez; Eric Lainé; Christophe Hano; Serge Pilard; Akram Hijazi; Assem El Kak; François Mesnard. 2021. "NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax." Molecules 26, no. 3: 767.

Journal article
Published: 02 July 2020 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Mature fruits (i.e., achenes) of milk thistle (Silybum marianum (L.) Gaertn., Asteraceae) accumulate high amounts of silymarin (SILM), a complex mixture of bioactive flavonolignans deriving from taxifolin. Their biological activities in relation with human health promotion and disease prevention are well described. However, the conditions of their biosynthesis in planta are still obscure. To fill this gap, fruit development stages were first precisely defined to study the accumulation kinetics of SILM constituents during fruit ripening. The accumulation profiles of the SILM components during fruit maturation were determined using the LC-MS analysis of these defined developmental phases. The kinetics of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and peroxidase (POX) activities suggest in situ biosynthesis of SILM from l-Phenylalanine during fruit maturation rather than a transport of precursors to the achene. In particular, in contrast to laccase activity, POX activity was associated with the accumulation of silymarin, thus indicating a possible preferential involvement of peroxidase(s) in the oxidative coupling step leading to flavonolignans. Reference genes have been identified, selected and validated to allow accurate gene expression profiling of candidate biosynthetic genes (PAL, CAD, CHS, F3H, F3'H and POX) related to SILM accumulation. Gene expression profiles were correlated with SILM accumulation kinetic and preferential location in pericarp during S. marianum fruit maturation, reaching maximum biosynthesis when desiccation occurs, thus reinforcing the hypothesis of an in situ biosynthesis. This observation led us to consider the involvement of abscisic acid (ABA), a key phytohormone in the control of fruit ripening process. ABA accumulation timing and location during milk thistle fruit ripening appeared in line with a potential regulation of the SLIM accumulation. A possible transcriptional regulation of SILM biosynthesis by ABA was supported by the presence of ABA-responsive cis-acting elements in the promoter regions of the SILM biosynthetic genes studied. These results pave the way for a better understanding of the biosynthetic regulation of SILM during the maturation of S. marianum fruit and offer important insights to better control the production of these medicinally important compounds.

ACS Style

Samantha Drouet; Duangjai Tungmunnithum; Éric Lainé; Christophe Hano. Gene Expression Analysis and Metabolite Profiling of Silymarin Biosynthesis during Milk Thistle (Silybum marianum (L.) Gaertn.) Fruit Ripening. International Journal of Molecular Sciences 2020, 21, 4730 .

AMA Style

Samantha Drouet, Duangjai Tungmunnithum, Éric Lainé, Christophe Hano. Gene Expression Analysis and Metabolite Profiling of Silymarin Biosynthesis during Milk Thistle (Silybum marianum (L.) Gaertn.) Fruit Ripening. International Journal of Molecular Sciences. 2020; 21 (13):4730.

Chicago/Turabian Style

Samantha Drouet; Duangjai Tungmunnithum; Éric Lainé; Christophe Hano. 2020. "Gene Expression Analysis and Metabolite Profiling of Silymarin Biosynthesis during Milk Thistle (Silybum marianum (L.) Gaertn.) Fruit Ripening." International Journal of Molecular Sciences 21, no. 13: 4730.

Journal article
Published: 14 August 2019 in Antioxidants
Reads 0
Downloads 0

Silybum marianum (L.) Gaertn. (aka milk thistle) constitutes the source of silymarin (SILM), a mixture of different flavonolignans and represents a unique model for their extraction. Here we report on the development and validation of an ultrasound-assisted extraction (UAE) method of S. marianum flavonolignans follow by their quantification using LC system. The optimal conditions of this UAE method were: aqueous EtOH 54.5% (v/v) as extraction solvent, with application of an ultrasound (US) frequency of 36.6 kHz during 60 min at 45 °C with a liquid to solid ratio of 25:1 mL/g dry weight (DW). Following its optimization using a full factorial design, the extraction method was validated according to international standards of the association of analytical communities (AOAC) to ensure precision and accuracy in the quantitation of each component of the SILM mixture. The efficiency of this UAE was compared with maceration protocol. Here, the optimized and validated conditions of the UAE allowed the highest extraction yields of SILM and its constituents in comparison to maceration. During UAE, the antioxidant capacity of the extracts was retained, as confirmed by the in vitro assays CUPRAC (cupric ion reducing antioxidant capacity) and inhibition of AGEs (advanced glycation end products). The skin anti-aging potential of the extract obtained by UAE was also confirmed by the strong in vitro cell-free inhibition capacity of both collagenase and elastase. To summarize, the UAE procedure presented here is a green and efficient method for the extraction and quantification of SILM and its constituents from the fruits of S. marianum, making it possible to generate extracts with attractive antioxidant and anti-aging activities for future cosmetic applications.

ACS Style

Samantha Drouet; Emilie A. Leclerc; Laurine Garros; Duangjai Tungmunnithum; Atul Kabra; Bilal Haider Abbasi; Éric Lainé; Christophe Hano. A Green Ultrasound-Assisted Extraction Optimization of the Natural Antioxidant and Anti-Aging Flavonolignans from Milk Thistle Silybum marianum (L.) Gaertn. Fruits for Cosmetic Applications. Antioxidants 2019, 8, 304 .

AMA Style

Samantha Drouet, Emilie A. Leclerc, Laurine Garros, Duangjai Tungmunnithum, Atul Kabra, Bilal Haider Abbasi, Éric Lainé, Christophe Hano. A Green Ultrasound-Assisted Extraction Optimization of the Natural Antioxidant and Anti-Aging Flavonolignans from Milk Thistle Silybum marianum (L.) Gaertn. Fruits for Cosmetic Applications. Antioxidants. 2019; 8 (8):304.

Chicago/Turabian Style

Samantha Drouet; Emilie A. Leclerc; Laurine Garros; Duangjai Tungmunnithum; Atul Kabra; Bilal Haider Abbasi; Éric Lainé; Christophe Hano. 2019. "A Green Ultrasound-Assisted Extraction Optimization of the Natural Antioxidant and Anti-Aging Flavonolignans from Milk Thistle Silybum marianum (L.) Gaertn. Fruits for Cosmetic Applications." Antioxidants 8, no. 8: 304.

Original article
Published: 29 April 2019 in Planta
Reads 0
Downloads 0

The involvement of a WRKY transcription factor in the regulation of lignan biosynthesis in flax using a hairy root system is described. Secoisolariciresinol is the main flax lignan synthesized by action of LuPLR1 (pinoresinol-lariciresinol reductase 1). LuPLR1 gene promoter deletion experiments have revealed a promoter region containing W boxes potentially responsible for the response to Fusarium oxysporum. W boxes are bound by WRKY transcription factors that play a role in the response to stress. A candidate WRKY transcription factor, LuWRKY36, was isolated from both abscisic acid and Fusarium elicitor-treated flax cell cDNA libraries. This transcription factors contains two WRKY DNA-binding domains and is a homolog of AtWRKY33. Different approaches confirmed LuWRKY36 binding to a W box located in the LuPLR1 promoter occurring through a unique direct interaction mediated by its N-terminal WRKY domain. Our results propose that the positive regulator action of LuWRKY36 on the LuPLR1 gene regulation and lignan biosynthesis in response to biotic stress is positively mediated by abscisic acid and inhibited by ethylene. Additionally, we demonstrate a differential Fusarium elicitor response in susceptible and resistant flax cultivars, seen as a faster and stronger LuPLR1 gene expression response accompanied with higher secoisolariciresinol accumulation in HR of the resistant cultivar.

ACS Style

Lucija Markulin; Cyrielle Corbin; Sullivan Renouard; Samantha Drouet; Charlène Durpoix; Charlotte Mathieu; Tatiana Lopez; Daniel Auguin; Christophe Hano; Éric Lainé. Characterization of LuWRKY36, a flax transcription factor promoting secoisolariciresinol biosynthesis in response to Fusarium oxysporum elicitors in Linum usitatissimum L. hairy roots. Planta 2019, 250, 347 -366.

AMA Style

Lucija Markulin, Cyrielle Corbin, Sullivan Renouard, Samantha Drouet, Charlène Durpoix, Charlotte Mathieu, Tatiana Lopez, Daniel Auguin, Christophe Hano, Éric Lainé. Characterization of LuWRKY36, a flax transcription factor promoting secoisolariciresinol biosynthesis in response to Fusarium oxysporum elicitors in Linum usitatissimum L. hairy roots. Planta. 2019; 250 (1):347-366.

Chicago/Turabian Style

Lucija Markulin; Cyrielle Corbin; Sullivan Renouard; Samantha Drouet; Charlène Durpoix; Charlotte Mathieu; Tatiana Lopez; Daniel Auguin; Christophe Hano; Éric Lainé. 2019. "Characterization of LuWRKY36, a flax transcription factor promoting secoisolariciresinol biosynthesis in response to Fusarium oxysporum elicitors in Linum usitatissimum L. hairy roots." Planta 250, no. 1: 347-366.

Review
Published: 20 March 2019 in Planta
Reads 0
Downloads 0

This paper provides an overview on activity, stereospecificity, expression and regulation of pinoresinol–lariciresinol reductases in plants. These enzymes are shared by the pathways to all 8–8′ lignans derived from pinoresinol. Pinoresinol–lariciresinol reductases (PLR) are enzymes involved in the lignan biosynthesis after the initial dimerization of two monolignols. They catalyze two successive reduction steps leading to the production of lariciresinol or secoisolariciresinol from pinoresinol. Two secoisolariciresinol enantiomers can be synthetized with different fates. Depending on the plant species, these enantiomers are either final products (e.g., in the flaxseed where it is stored after glycosylation) or are the starting point for the synthesis of a wide range of lignans, among which the aryltetralin type lignans are used to semisynthesize anticancer drugs such as Etoposide®. Thus, the regulation of the gene expression of PLRs as well as the possible specificities of these reductases for one reduction step or one enantiomer are key factors to fine-tune the lignan synthesis. Results published in the last decade have shed light on the presence of more than one PLR in each plant and revealed various modes of action. Nevertheless, there are not many results published on the PLRs and most of them were obtained in a limited range of species. Indeed, a number of them deal with wild and cultivated flax belonging to the genus Linum. Despite the occurrence of lignans in bryophytes, pteridophytes and monocots, data on PLRs in these taxa are still missing and indeed the whole diversity of PLRs is still unknown. This review summarizes the data, published mainly in the last decade, on the PLR gene expression, enzymatic activity and biological function.

ACS Style

Lucija Markulin; Cyrielle Corbin; Sullivan Renouard; Samantha Drouet; Laurent Gutierrez; Ivan Mateljak; Daniel Auguin; Christophe Hano; Elisabeth Fuss; Eric Lainé. Pinoresinol–lariciresinol reductases, key to the lignan synthesis in plants. Planta 2019, 249, 1695 -1714.

AMA Style

Lucija Markulin, Cyrielle Corbin, Sullivan Renouard, Samantha Drouet, Laurent Gutierrez, Ivan Mateljak, Daniel Auguin, Christophe Hano, Elisabeth Fuss, Eric Lainé. Pinoresinol–lariciresinol reductases, key to the lignan synthesis in plants. Planta. 2019; 249 (6):1695-1714.

Chicago/Turabian Style

Lucija Markulin; Cyrielle Corbin; Sullivan Renouard; Samantha Drouet; Laurent Gutierrez; Ivan Mateljak; Daniel Auguin; Christophe Hano; Elisabeth Fuss; Eric Lainé. 2019. "Pinoresinol–lariciresinol reductases, key to the lignan synthesis in plants." Planta 249, no. 6: 1695-1714.

Journal article
Published: 27 February 2019 in Plants
Reads 0
Downloads 0

Painted nettle (Plectranthus scutellarioides (L.) R.Br.) is an ornamental plant belonging to Lamiaceae family, native of Asia. Its leaves constitute one of the richest sources of trans-rosmarinic acid, a well-known antioxidant and antimicrobial phenolic compound. These biological activities attract interest from the cosmetic industry and the demand for the development of green sustainable extraction processes. Here, we report on the optimization and validation of an ultrasound-assisted extraction (USAE) method using ethanol as solvent. Following preliminary single factor experiments, the identified limiting extraction parameters (i.e., ultrasound frequency, extraction duration, and ethanol concentration) were further optimized using a full factorial design approach. The method was then validated following the recommendations of the association of analytical communities (AOAC) to ensure the precision and accuracy of the method used to quantify trans-rosmarinic acid. Highest trans-rosmarinic acid content was obtained using pure ethanol as extraction solvent following a 45-minute extraction in an ultrasound bath operating at an ultrasound frequency of 30 kHz. The antioxidant (in vitro radical scavenging activity) and antimicrobial (directed toward Staphylococcus aureus ACTT6538) activities were significantly correlated with the trans-rosmarinic acid concentration of the extract evidencing that these key biological activities were retained following the extraction using this validated method. Under these conditions, 110.8 mg/g DW of trans-rosmarinic acid were extracted from lyophilized P. scutellarioides leaves as starting material evidencing the great potential of this renewable material for cosmetic applications. Comparison to other classical extraction methods evidenced a clear benefit of the present USAE method both in terms of yield and extraction duration.

ACS Style

Duangjai Tungmunnithum; Laurine Garros; Samantha Drouet; Sullivan Renouard; Eric Lainé; Christophe Hano. Green Ultrasound Assisted Extraction of trans Rosmarinic Acid from Plectranthus scutellarioides (L.) R.Br. Leaves. Plants 2019, 8, 50 .

AMA Style

Duangjai Tungmunnithum, Laurine Garros, Samantha Drouet, Sullivan Renouard, Eric Lainé, Christophe Hano. Green Ultrasound Assisted Extraction of trans Rosmarinic Acid from Plectranthus scutellarioides (L.) R.Br. Leaves. Plants. 2019; 8 (3):50.

Chicago/Turabian Style

Duangjai Tungmunnithum; Laurine Garros; Samantha Drouet; Sullivan Renouard; Eric Lainé; Christophe Hano. 2019. "Green Ultrasound Assisted Extraction of trans Rosmarinic Acid from Plectranthus scutellarioides (L.) R.Br. Leaves." Plants 8, no. 3: 50.

Journal article
Published: 14 October 2018 in Molecules
Reads 0
Downloads 0

Flaxseeds are a functional food representing, by far, the richest natural grain source of lignans, and accumulate substantial amounts of other health beneficial phenolic compounds (i.e., flavonols, hydroxycinnamic acids). This specific accumulation pattern is related to their numerous beneficial effects on human health. However, to date, little data is available concerning the relative impact of genetic and geographic parameters on the phytochemical yield and composition. Here, the major influence of the cultivar over geographic parameters on the flaxseed phytochemical accumulation yield and composition is evidenced. The importance of genetic parameters on the lignan accumulation was further confirmed by gene expression analysis monitored by RT-qPCR. The corresponding antioxidant activity of these flaxseed extracts was evaluated, both in vitro, using ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), and iron chelating assays, as well as in vivo, by monitoring the impact of UV-induced oxidative stress on the lipid membrane peroxidation of yeast cells. Our results, both the in vitro and in vivo studies, confirm that flaxseed extracts are an effective protector against oxidative stress. The results point out that secoisolariciresinol diglucoside, caffeic acid glucoside, and p-coumaric acid glucoside are the main contributors to the antioxidant capacity. Considering the health benefits of these compounds, the present study demonstrates that the flaxseed cultivar type could greatly influence the phytochemical intakes and, therefore, the associated biological activities. We recommend that this crucial parameter be considered in epidemiological studies dealing with flaxseeds.

ACS Style

Laurine Garros; Samantha Drouet; Cyrielle Corbin; Cédric Decourtil; Thibaud Fidel; Julie Lebas De Lacour; Emilie A. Leclerc; Sullivan Renouard; Duangjai Tungmunnithum; Joël Doussot; Bilal Haider Abassi; Benoit Maunit; Éric Lainé; Ophélie Fliniaux; François Mesnard; Christophe Hano. Insight into the Influence of Cultivar Type, Cultivation Year, and Site on the Lignans and Related Phenolic Profiles, and the Health-Promoting Antioxidant Potential of Flax (Linum usitatissimum L.) Seeds. Molecules 2018, 23, 2636 .

AMA Style

Laurine Garros, Samantha Drouet, Cyrielle Corbin, Cédric Decourtil, Thibaud Fidel, Julie Lebas De Lacour, Emilie A. Leclerc, Sullivan Renouard, Duangjai Tungmunnithum, Joël Doussot, Bilal Haider Abassi, Benoit Maunit, Éric Lainé, Ophélie Fliniaux, François Mesnard, Christophe Hano. Insight into the Influence of Cultivar Type, Cultivation Year, and Site on the Lignans and Related Phenolic Profiles, and the Health-Promoting Antioxidant Potential of Flax (Linum usitatissimum L.) Seeds. Molecules. 2018; 23 (10):2636.

Chicago/Turabian Style

Laurine Garros; Samantha Drouet; Cyrielle Corbin; Cédric Decourtil; Thibaud Fidel; Julie Lebas De Lacour; Emilie A. Leclerc; Sullivan Renouard; Duangjai Tungmunnithum; Joël Doussot; Bilal Haider Abassi; Benoit Maunit; Éric Lainé; Ophélie Fliniaux; François Mesnard; Christophe Hano. 2018. "Insight into the Influence of Cultivar Type, Cultivation Year, and Site on the Lignans and Related Phenolic Profiles, and the Health-Promoting Antioxidant Potential of Flax (Linum usitatissimum L.) Seeds." Molecules 23, no. 10: 2636.

Communication
Published: 10 October 2018 in Molecules
Reads 0
Downloads 0

A selective acylation protocol using cerium chloride (CeCl3) as catalyst was applied to functionalize silybinin (1), a natural antioxidant flavonolignan from milk thistle fruit, in order to increase its solubility in lipophilic media while retaining its strong antioxidant activity. The selective esterification of 1 at the position 3-OH with a palmitate acyl chain leading to the formation of the 3-O-palmitoyl-silybin (2) was confirmed by both mass spectroscopy (MS) and nuclear magnetic resonance (NMR) analyses. The antioxidant activity of 1 was at least retained and even increased with the CUPRAC assay designed to estimate the antioxidant activity of both hydrophilic and lipophilic compounds. Finally, the 3-O-palmitoylation of 1, resulting in the formation of 2, also increased its anti-lipoperoxidant activity (i.e., inhibition of conjugated diene production) in two different lipophilic media (bulk oil and o/w emulsion) subjected to accelerated storage test.

ACS Style

Samantha Drouet; Joël Doussot; Laurine Garros; David Mathiron; Solène Bassard; Alain Favre-Réguillon; Roland Molinié; Éric Lainé; Christophe Hano. Selective Synthesis of 3-O-Palmitoyl-Silybin, a New-to-Nature Flavonolignan with Increased Protective Action against Oxidative Damages in Lipophilic Media. Molecules 2018, 23, 2594 .

AMA Style

Samantha Drouet, Joël Doussot, Laurine Garros, David Mathiron, Solène Bassard, Alain Favre-Réguillon, Roland Molinié, Éric Lainé, Christophe Hano. Selective Synthesis of 3-O-Palmitoyl-Silybin, a New-to-Nature Flavonolignan with Increased Protective Action against Oxidative Damages in Lipophilic Media. Molecules. 2018; 23 (10):2594.

Chicago/Turabian Style

Samantha Drouet; Joël Doussot; Laurine Garros; David Mathiron; Solène Bassard; Alain Favre-Réguillon; Roland Molinié; Éric Lainé; Christophe Hano. 2018. "Selective Synthesis of 3-O-Palmitoyl-Silybin, a New-to-Nature Flavonolignan with Increased Protective Action against Oxidative Damages in Lipophilic Media." Molecules 23, no. 10: 2594.

Review
Published: 01 May 2018 in Cosmetics
Reads 0
Downloads 0

A number of approaches can be implemented to ensure plant-based material authentication for cosmetic applications. Doing this requires knowledge and data dealing with botany, molecular biology, and analytical chemistry, the main techniques of which are described here. A comprehensive and critical view of the methods is provided with comments as well as examples of their application domains.

ACS Style

Samantha Drouet; Laurine Garros; Christophe Hano; Duangjai Tungmunnithum; Sullivan Renouard; Daniel Hagège; Benoît Maunit; Éric Lainé. A Critical View of Different Botanical, Molecular, and Chemical Techniques Used in Authentication of Plant Materials for Cosmetic Applications. Cosmetics 2018, 5, 30 .

AMA Style

Samantha Drouet, Laurine Garros, Christophe Hano, Duangjai Tungmunnithum, Sullivan Renouard, Daniel Hagège, Benoît Maunit, Éric Lainé. A Critical View of Different Botanical, Molecular, and Chemical Techniques Used in Authentication of Plant Materials for Cosmetic Applications. Cosmetics. 2018; 5 (2):30.

Chicago/Turabian Style

Samantha Drouet; Laurine Garros; Christophe Hano; Duangjai Tungmunnithum; Sullivan Renouard; Daniel Hagège; Benoît Maunit; Éric Lainé. 2018. "A Critical View of Different Botanical, Molecular, and Chemical Techniques Used in Authentication of Plant Materials for Cosmetic Applications." Cosmetics 5, no. 2: 30.

Journal article
Published: 14 April 2018 in Molecules
Reads 0
Downloads 0

Fruits of Silybum marianum (L.) Gaernt are the main source of taxifolin derived flavonolignans. Together, these molecules constitute a mixture called silymarin with many useful applications for cosmetic and pharmaceutic industries. Here, a validated method for the separation of the silymarin constituents has been developed to ensure precision and accuracy in their quantification. Each compound was separated with a high reproducibility. Precision and repeatability of the quantification method were validated according to the AOAC recommendations. The method was then applied to study the natural variability of wild accessions of S. marianum. Analysis of the variation in the fruits composition of these 12 accessions from Pakistan evidenced a huge natural diversity. Correlation analysis suggested a synergistic action of the different flavonolignans to reach the maximal antioxidant activity, as determined by cupric ion reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP) assays. Principal component analysis (PCA) separated the 12 accessions into three distinct groups that were differing from their silymarin contents, whereas hierarchical clustering analysis (HCA) evidenced strong variations in their silymarin composition, leading to the identification of new silybin-rich chemotypes. These results proved that the present method allows for an efficient separation and quantification of the main flavonolignans with potent antioxidant activities.

ACS Style

Samantha Drouet; Bilal Haider Abbasi; Annie Falguières; Waqar Ahmad; Sumaira; Clothilde Ferroud; Joël Doussot; Jean Raymond Vanier; Éric Lainé; Christophe Hano. Single Laboratory Validation of a Quantitative Core Shell-Based LC Separation for the Evaluation of Silymarin Variability and Associated Antioxidant Activity of Pakistani Ecotypes of Milk Thistle (Silybum Marianum L.). Molecules 2018, 23, 904 .

AMA Style

Samantha Drouet, Bilal Haider Abbasi, Annie Falguières, Waqar Ahmad, Sumaira, Clothilde Ferroud, Joël Doussot, Jean Raymond Vanier, Éric Lainé, Christophe Hano. Single Laboratory Validation of a Quantitative Core Shell-Based LC Separation for the Evaluation of Silymarin Variability and Associated Antioxidant Activity of Pakistani Ecotypes of Milk Thistle (Silybum Marianum L.). Molecules. 2018; 23 (4):904.

Chicago/Turabian Style

Samantha Drouet; Bilal Haider Abbasi; Annie Falguières; Waqar Ahmad; Sumaira; Clothilde Ferroud; Joël Doussot; Jean Raymond Vanier; Éric Lainé; Christophe Hano. 2018. "Single Laboratory Validation of a Quantitative Core Shell-Based LC Separation for the Evaluation of Silymarin Variability and Associated Antioxidant Activity of Pakistani Ecotypes of Milk Thistle (Silybum Marianum L.)." Molecules 23, no. 4: 904.

Journal article
Published: 26 March 2018 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands.

ACS Style

Sullivan Renouard; Cyrielle Corbin; Samantha Drouet; Barbara Medvedec; Joël Doussot; Cyril Colas; Benoit Maunit; Avninder S. Bhambra; Eric Gontier; Nathalie Jullian; François Mesnard; Michèle Boitel; Bilal Haider Abbasi; Randolph R. J. Arroo; Eric Lainé; Christophe Hano. Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans. International Journal of Molecular Sciences 2018, 19, 990 .

AMA Style

Sullivan Renouard, Cyrielle Corbin, Samantha Drouet, Barbara Medvedec, Joël Doussot, Cyril Colas, Benoit Maunit, Avninder S. Bhambra, Eric Gontier, Nathalie Jullian, François Mesnard, Michèle Boitel, Bilal Haider Abbasi, Randolph R. J. Arroo, Eric Lainé, Christophe Hano. Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans. International Journal of Molecular Sciences. 2018; 19 (4):990.

Chicago/Turabian Style

Sullivan Renouard; Cyrielle Corbin; Samantha Drouet; Barbara Medvedec; Joël Doussot; Cyril Colas; Benoit Maunit; Avninder S. Bhambra; Eric Gontier; Nathalie Jullian; François Mesnard; Michèle Boitel; Bilal Haider Abbasi; Randolph R. J. Arroo; Eric Lainé; Christophe Hano. 2018. "Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans." International Journal of Molecular Sciences 19, no. 4: 990.

Journal article
Published: 10 March 2014 in Molecules
Reads 0
Downloads 0

Flax (Linum usitatissimum L.) seeds are widely used for oil extraction and the cold-pressed flaxseed (or linseed) cakes obtained during this process constitute a valuable by-product. The flavonol herbacetin diglucoside (HDG) has been previously reported as a constituent of the flaxseed lignan macromolecule linked through ester bonds to the linker molecule hydroxymethylglutaric acid. In this context, the development and validation of a new approach using microwave-assisted extraction (MAE) of HDG from flaxseed cakes followed by quantification with a reverse-phase HPLC system with UV detection was purposed. The experimental parameters affecting the HDG extraction yield, such as microwave power, extraction time and sodium hydroxide concentration, from the lignan macromolecule were optimized. A maximum HDG concentration of 5.76 mg/g DW in flaxseed cakes was measured following an irradiation time of 6 min, for a microwave power of 150 W using a direct extraction in 0.1 M NaOH in 70% (v/v) aqueous methanol. The optimized method was proven to be rapid and reliable in terms of precision, repeatability, stability and accuracy for the extraction of HDG. Comparison with a conventional extraction method demonstrated that MAE is more effective and less time-consuming.

ACS Style

Ophélie Fliniaux; Cyrielle Corbin; Aina Ramsay; Sullivan Renouard; Vickram Beejmohun; Joël Doussot; Annie Falguieres; Clotilde Ferroud; Frédéric Lamblin; Eric Lainé; Albrecht Roscher; Eric Grand; François Mesnard; Christophe Hano. Microwave-Assisted Extraction of Herbacetin Diglucoside from Flax (Linum usitatissimum L.) Seed Cakes and Its Quantification using an RP-HPLC-UV System. Molecules 2014, 19, 3025 -3037.

AMA Style

Ophélie Fliniaux, Cyrielle Corbin, Aina Ramsay, Sullivan Renouard, Vickram Beejmohun, Joël Doussot, Annie Falguieres, Clotilde Ferroud, Frédéric Lamblin, Eric Lainé, Albrecht Roscher, Eric Grand, François Mesnard, Christophe Hano. Microwave-Assisted Extraction of Herbacetin Diglucoside from Flax (Linum usitatissimum L.) Seed Cakes and Its Quantification using an RP-HPLC-UV System. Molecules. 2014; 19 (3):3025-3037.

Chicago/Turabian Style

Ophélie Fliniaux; Cyrielle Corbin; Aina Ramsay; Sullivan Renouard; Vickram Beejmohun; Joël Doussot; Annie Falguieres; Clotilde Ferroud; Frédéric Lamblin; Eric Lainé; Albrecht Roscher; Eric Grand; François Mesnard; Christophe Hano. 2014. "Microwave-Assisted Extraction of Herbacetin Diglucoside from Flax (Linum usitatissimum L.) Seed Cakes and Its Quantification using an RP-HPLC-UV System." Molecules 19, no. 3: 3025-3037.

Article original
Published: 01 August 2007 in Phytothérapie
Reads 0
Downloads 0

Les graines du lin contiennent des teneurs très élevées en composés phénoliques, les lignanes, en particulier en sécoisolaricirésinol diglucoside (SDG). Ses propriétés antioxydantes et de liaison aux récepteurs oestrogéniques pourraient expliquer ses activités biologiques en rapport avec la prévention de certains cancers et des maladies cardiovasculaires. On observe cependant des résultats contradictoires parmi les études épidémiologiques publiées à ce jour. Toutefois, malgré la qualification de phytoestrogène du SDG, il n’a jamais été observé de stimulation de croissance de cellules cancéreuses. La connaissance du devenir des lignanes ingérés et de leurs interactions possibles avec notre métabolisme, en particulier hormonal, progresse. La récente disponibilité de produit purifié permet maintenant d’en étudier les effets in vitro. Linseed contains an high lignan content, peculiarly secoisolariciresinol diglucoside (SDG). Antioxydant properties as well as it’s ability to bind estrogen receptors could explain its biological activities and preventive action against cancers and cardiovascular disease. Epidemiological studies have yielded contradictory results on the benefits of SDG. Nevertheless it is worth to note that, although classified as phyoestrogen, no detrimental effect of SDG (such as tumorous cell growth stimulation) has been observed. Our knowledge is increasing in the field of lignan intake and metabolism and further interaction with our metabolism, particularly the sexual hormones one. The recent availability of purified products now allows in vitro studies to better understand the biological activities of lignans.

ACS Style

E. Lainé; Christophe Hano; F. Lamblin. Les lignanes phytoestrogènes du lin sont-ils des bienfaiteurs méconnus? Phytothérapie 2007, 5, 121 -128.

AMA Style

E. Lainé, Christophe Hano, F. Lamblin. Les lignanes phytoestrogènes du lin sont-ils des bienfaiteurs méconnus? Phytothérapie. 2007; 5 (3):121-128.

Chicago/Turabian Style

E. Lainé; Christophe Hano; F. Lamblin. 2007. "Les lignanes phytoestrogènes du lin sont-ils des bienfaiteurs méconnus?" Phytothérapie 5, no. 3: 121-128.

Original article
Published: 31 May 2006 in Planta
Reads 0
Downloads 0

The transcription activity of the pinoresinol–lariciresinol reductase (PLR) gene of Linum usitatissimum (so-called LuPLR), a key gene in lignan synthesis, was studied by RT-PCR and promoter–reporter transgenesis. The promoter was found to drive transcription of a GUSint reporter gene in the seed coats during the flax seed development. This fitted well with the tissue localization monitored by semi-quantitative RT-PCR of LuPLR expression. Accumulation of the main flax lignan secoisolariciresinol diglucoside was coherent with LuPLR expression during seed development. This three-way approach demonstrated that the LuPLR gene is expressed in the seed coat of flax seeds, and that the synthesis of PLR enzyme occurs where flax main lignan is found stored in mature seeds, confirming its involvement in SDG synthesis.

ACS Style

Christophe Hano; I. Martin; O. Fliniaux; Baptiste Legrand; Laurent Gutierrez; R. R. J. Arroo; F. Mesnard; F. Lamblin; E. Lainé. Pinoresinol–lariciresinol reductase gene expression and secoisolariciresinol diglucoside accumulation in developing flax (Linum usitatissimum) seeds. Planta 2006, 224, 1291 -1301.

AMA Style

Christophe Hano, I. Martin, O. Fliniaux, Baptiste Legrand, Laurent Gutierrez, R. R. J. Arroo, F. Mesnard, F. Lamblin, E. Lainé. Pinoresinol–lariciresinol reductase gene expression and secoisolariciresinol diglucoside accumulation in developing flax (Linum usitatissimum) seeds. Planta. 2006; 224 (6):1291-1301.

Chicago/Turabian Style

Christophe Hano; I. Martin; O. Fliniaux; Baptiste Legrand; Laurent Gutierrez; R. R. J. Arroo; F. Mesnard; F. Lamblin; E. Lainé. 2006. "Pinoresinol–lariciresinol reductase gene expression and secoisolariciresinol diglucoside accumulation in developing flax (Linum usitatissimum) seeds." Planta 224, no. 6: 1291-1301.