Background/Objectives: Urinary tract infections (UTIs) are infections that involve the urethra, bladder, and, in much more severe cases, even kidneys. These infections represent one of the most common diseases worldwide. Various pathogens are responsible for this condition, the most common being Escherichia coli (E. coli). Bromelain is a proteolytic complex obtained from the stem and stalk of Ananas comosus (L.) Merr. showing several beneficial activities. In addition to bromelain, N-acetylcysteine (NAC) has also been used. Methods: The purpose of this experiment was to evaluate the antibacterial, anti-motility, and anti-biofilm effects of a new polyenzymatic complex (DIF17BRO^®) in combination with NAC (the Formulation) on various strains of E. coli isolated from patients with UTIs. Subsequently, the anti-inflammatory and antioxidant effects of the Formulation were studied in an ex vivo model of cystitis, using bladder samples from mice exposed to E. coli lipopolysaccharide (LPS). Results: Our results showed that the Formulation significantly affects the capability of bacteria to form biofilm and reduces the bacteria amount in the mature biofilm. Moreover, it combines the interesting properties of NAC and a polyenzyme plant complex based on bromelain in a right dose to affect the E. coli adhesion capability. Finally, the Formulation exhibited protective effects, as confirmed by the inhibitory activities on multiple inflammatory and oxidative stress-related pathways on bladder specimens exposed to LPS. Conclusions: This blend of active compounds could represent a promising and versatile approach to use to overcome the limitations associated with conventional therapies.

Background: antimicrobial resistance represents a critical issue leading to delayed wound healing; hence, it is necessary to develop novel strategies to address this phenomenon. Objectives: this study aimed to explore the antimicrobial/anti-virulence action of Methylglyoxal-MGO alone or combined with novel technologies such as Light-Emitting Diodes-LED and Complex Magnetic Fields-CMFs against resistant clinical strains isolated from chronic wounds. Methods: characterized planktonic Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans isolates were used. Antimicrobial activity was evaluated by measuring optical density, Colony Forming Units-CFU, and synergy between MGO/LED or CMFs. Cellular membrane permeability by propidium iodide fluorescence and fluidity by Laurdan generalized polarization measurements were performed. P. aeruginosa motility was tested using the soft agar method. A docking study was performed to evaluate the possible interaction between MGO and urease in P. aeruginosa. Results: single/combined treatments showed significant antimicrobial activity. Major CFU reduction was detected after CMFs/MGO+CMFs application on C. albicans. Treatments exhibited significant changes in membrane permeability and fluidity. The treatments decreased P. aeruginosa motility with a major reduction after LED application. Docking analysis showed that MGO could bind with P. aeruginosa urease leading to defective folding and functional alterations. Conclusions: the results suggest that these treatments could represent promising and green therapeutic solutions against resistant isolates from chronic wounds.

Chronic wound infections associated with resistant polymicrobial biofilms are often refractory to conventional therapies with sustained healing time. This study evaluated the efficacy of non-antibiotic treatments including Methylglyoxal—MGO—Light-Emitting Diode—LED—and Complex Magnetic Fields—CMFs—alone/combined against the biofilms of two polymicrobial mixes (MIX 1, MIX 2) containing S. aureus, P. aeruginosa and C. albicans using the Lubbock chronic wound biofilm model. At 24 h after treatment, the effects were evaluated by (i) CFU/mg reduction, (ii) Confocal Laser Scanning Microscopy—CLSM—and (iii) Scanning Electron Microscopy—SEM. All treatments significantly reduced biofilms in terms of CFU/mg in both mixes versus the controls, 24 h after treatment. MGO showed remarkable activity, especially against P. aeruginosa. In MIX 1, LED/MGO + LED was highly effective against C. albicans. The combinations MGO + LED/MGO + CMFs enhanced the antibiofilm activity compared to each single treatment against S. aureus and P. aeruginosa, in both MIX1/MIX2. CLSM and SEM analysis showed biofilm disaggregation and reduction in cell viability with combined treatments, and Candida hyphal inhibition after CMFs application. In conclusion, the results demonstrate that MGO, alone or combined with LED or CMFs, shows high efficacy against resistant biofilms in the LCWB model 24 h after treatment, and encourage further studies on potential non-antibiotic and eco-friendly strategies as future alternative therapeutic approaches for chronic wound infections.

Biofilm plays a crucial role in the pathogenesis and chronicity of urinary tract infections (UTIs). The present work aimed to evaluate the anti-biofilm effects of Formulation (DIF17BRO^® plus NAC) in combination with ciprofloxacin (CPX) on Escherichia coli strains. The antimicrobial activity of ciprofloxacin was evaluated by minimum inhibitory concentration (MIC) determination, and the antibiofilm effects of ciprofloxacin alone and combined with Formulation were evaluated on E. coli ATCC700926, E. coli ATCC10536, E. coli PNT, and E. coli PCA mature biofilms in terms of CFU/mL and biomass quantifications. Moreover, the potential protective effects of Formulation plus ciprofloxacin was tested in a Galleria mellonella in vivo infection assay. Our results underlined the increased microbial reduction in the mature biofilm in the presence of the combination Formulation and CPX, even at a lower concentration of CPX. Formulation increased the percentage of biofilm biomass reduction, inducing a disruption of the biofilm structure itself. Our present findings confirm that MIC CPX combined with Formulation also induced an antimicrobial effect in the G. mellonella assay. Formulation facilitated the perturbation of the biofilm polymeric matrix, enhancing the antibiotic penetration and its antimicrobial action on bacteria, underlining Formulation’s role as an enhancer of ciprofloxacin antibacterial action.

The microorganisms living around and inside the human host in an extraordinary way for their morphologies often remind, microscopically we find every day foodstuffs that in our tables. Background: Many food products “are similar” to bacteria, a sort of “convergent evolution” between the microscopic and the macroscopic. Here below, we present some “similarities“between bacteria and food suggesting “New Recipes” of the Abruzzo Region folk tradition in Central Italy, which handed down from father to son constituted the history of our land. We believe that the history of a people is the true identity of man and passion for their roots should never be circumvented. Passion that binds us to our traditions, the same that binds us to our work, microbiology, making it natural for us during lunch hour, in the laboratory, to speak of the particular similarities of bacteria found with the foods we consume daily. Methods: We compared to same macroscopic food products to the following bacteria: Helicobacter pylori, H. heilmannii, Neisseria meningitidis, Staphylococcus aureus, Leptospira interrogans, and Pseudomonas aeruginosa. Results: In particular, we identified for each microorganism “similarities“with food and proposed New Recipes inspired for their “assonance” to microbial morphology and behavior and hence, suggest the following: “the Conversion”, “the Unidentical Fusilli Twins”, “the Dangerous Peaches”, “the Greek Grape Cluster Cake”, “the Black Cuttlefish Spirals sauce” and “the Biofilm Green Zucchini”. Conclusions: Therefore, arriving at a “special” approach that wants to link the microscopic and macroscopic worlds underlining that each shape is not casual and that everything is connected.