We examined the effect of the double restriction-recovery cycle on survival, growth, and nutritional status of the freshwater prawn Macrobrachium borellii (Nobili, 1896), native to floodplain rivers of Argentina. The restriction period consisted of intermittent starvation (alternating four-day feeding with no feeding for four days) and a recovery period of continuous feeding following the restriction period. Survival was high over a 20-week trial with cycles of food scarcity. Prawns showed a slight impact on their growth throughout the entire first cycle of restriction-recovery; however, compensatory growth was not triggered. The reduction of growth was markedly more drastic after the second restriction period, but compensatory growth was not exhibited when the second recovery period was reestablished. Any possible impact of the double cycle of intermittent starvation on energy reserves and structure of the hepatopancreas was compensated by a posterior cycle of continuous feeding. The results demonstrated that M. borelli can successfully support long-term food scarcity throughout the juvenile and adult phases, at least intermittently and followed by a recovery period. The high nutritional flexibility is an adaptation to fluctuations in food availability and an advantage for successful ornamental aquaculture.

This work aimed to evaluate the effects of dietary phytic acid on the enzymatic activities of the hepatopancreas of the redclaw crayfish, Cherax quadricarinatus. For this purpose, a completely randomized in vitro trial was conducted with three phytic acid levels (0.56, 1.68, and 2.80%) and three phytase doses (0, 250, and 500 PU/kg DM). Solubilized protein, reducing sugars, and soluble phosphorus showed significant responses to the interaction between phytic acid and phytase . Only the main effects were detected on the released amino acids, in keeping with the main effects of alkaline protease activity, which are negatively affected by phytic acid and improved by phytase inclusion . Differences in released reducing sugars were attributed to a reduction in amylase activity by increased levels of phytic acid and not to cellulase activity, where only a negative trend of phytic acid was found . Phytic acid depresses calcium availability, which would explain the decrease in amylase activity. A 500 PU/kg DM dose improved amino acid, reduced sugars, and phosphorus release. These in vitro results might have in vivo implications for the digestibility of proteins, minerals, and energy. Further investigations are required to determine the chelated calcium effect on redclaw amylase activity, molting, and survival.