This manuscript reviews the advances in the application of biostimulants in grapevine (Vitis vinifera L.), emphasizing their physiological, agronomic, and quality impacts within a broader agricultural and scientific context. It highlights the evolution of biostimulant research and the theoretical frameworks that support their use, underscoring their growing relevance in sustainable viticulture as a response to environmental challenges and consumer demands for healthier production practices. By analyzing recent findings, the text outlines how biostimulants influence plant physiology, improve agronomic performance, and enhance fruit and wine quality, while also stressing the need for deeper understanding of their mechanisms of action and greater standardization in their application. The discussion suggests that advancing this field requires not only scientific attention but also an integrative vision that links innovation, sustainability, and practical implementation. Ultimately, the manuscript contributes to a more comprehensive appreciation of the role of biostimulants in viticulture, offering insights to guide future research and strategies for grapevine management and quality improvement.