Root-knot nematodes (RKN) are a serious threat to sustainable agroecosystems. They attack a wide range of crops all over the world and lead to significant yield losses. This chapter explores the use of bio-organic management strategies instead of harmful chemical control measures. We evaluate the possibilities of decreasing the RKN population by effectively using cropping practices such as crop rotation, resistant cultivars, and soil solarization. Most importantly, the chapter discusses about biological control agents (BCA) such as nematode-trapping fungi, endoparasitic fungi, Bacillus pumilus, Pasteuria penetrans, and other useful microorganisms to suppress the nematode population. Finally, we conclude by discussing the use of soil amendments such as botanicals, plant extracts, oil cakes, and other organic materials like manure and vermicompost to inhibit the RKN population and enhance soil health. This chapter provides us with valuable information on how to control nematode populations effectively and promote sustainable and environmentally friendly agricultural practices.