Galium aparine L., family Rubiaceae, is a widely spread species in the Galium genus. The herb of G. aparine is part of folk remedies and dietary supplements. In this study, we analyzed the chemical composition and immunomodulatory activities of G. aparine herb ethanolic extracts obtained from the plant material by maceration with 20%, 60% or 96% ethanol. The contents of hydroxycinnamic acid derivatives, flavonoids and polyphenols were determined spectrophotometrically, with extractives and polysaccharides quantified gravimetrically. The qualitative composition was studied using UHPLC-DAD-MS/MS analysis; isolation not previously described in G. aparine quercetin rhamnoglucoside was carried out through column chromatography, and the immunomodulatory activity of extracts was determined in the reaction of lymphocyte blast transformation. Major constitutes of extracts were iridoids, i.e., monotropein, 10-desacetylasperulosidic acid and asperulosidic acid; p-hydroxybenzoic acid; hydroxycinnamic acid derivatives, i.e., 3-O-caffeoylquinic, 5-O-caffeoylquinic, 3,4-O-dicaffeoylquinic, 3,5-O-dicaffeoylquinic, 4,5-O-dicaffeoylquinic acids and caffeic acid derivatives; flavonoids, i.e., rutin, quercetin 3-O-rhamnoglucoside-7-O-glucoside, and isorhamnetin 3-O-glucorhamnoside. Significantly, quercetin 3-O-rhamnoglucoside-7-O-glucoside was first isolated and identified in Galium species so far investigated. All G. aparine herb ethanolic extracts stimulate the transformational activity of immunocompetent blood cells, with 96% ethanolic extract being the most active. The data obtained necessitate further research into the mechanisms of immunomodulatory activity of extracts from G. aparine herb.

Extracts from aerial parts of G. aparine (cleavers) constitute a herbal remedy with monography in British Herbal Pharmacopeia. On the European market, there are several drugs and food supplements consisting of Galium extracts. In folk medicine, cleavers was used topically in Europe, Asia, and the Americas to treat skin diseases. In several remedies, cleavers is also listed as an immunomodulatory active herb influencing the defense response of the human body. The aim of this study was to investigate the immunostimulatory activity and antioxidant potential in vitro of a raw infusion of cleavers and bioactive fractions. The functional activity of lymphocytes in the reaction of the lymphocyte blast transformation (RLBT) method was used for immunomodulatory activity assays and direct scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), and hydrogen peroxide (H₂O₂) was chosen for the examination of antioxidant activity. It was shown that both the raw extract and fractions show significant immunostimulatory and scavenging activities. The obtained data partially justify the traditional use of cleavers as topical remedy for skin infections and for wounds.

The key objective of this study was to determine the yield and chemical composition of eight commercial samples and one collected sample of common vervain (Verbena officinalis L.) herb essential oil (EO) originating from seven different countries, and subsequently, to assess its potential for treating anxiety and depression. According to GC-MS analysis, 90 compounds were identified, 49 of which were discovered for the first time in V. officinalis EO. The plants with the highest oil content are from Greece (4.7 mL/kg) and South Carolina, USA (5.2 mL/kg). The chemical markers for the studied chemotypes of EO of V. officinalis are the terpenoids o-cymene, p-cymene, L-carvone, thymol, carvacrol, α-curcumin, hexahydrofarnesyl acetone, phytol, (E)-β-ionone, and phenylpropene anethole. The chemotype from the UK demonstrated the greatest affinity to the continuum under study; it has the highest levels of similarity—85.2% with the chemotype from Greece, 69.4% with the chemotype from the USA, 68.2% with the chemotype from Estonia (2), 58.7% with the chemotype from Germany, and 58.6% with the chemotype from Hungary. The chemotypes identified have the potential for use in the treatment of anxiety and depression.

The genus Alchemilla L. has about 1000 species in the world flora, of which 35 grow in Ukraine. The most common in Ukraine are A. flabellata Bus., A. phegophila Juz. and A. subrenata Bus. Despite the widespread distribution of species of the genus Alchemilla L. and the publication of a monograph on the herb of the collective species - Herba Alchemillae (Alchemiilla vulgaris L. sensu latiore) in the State Pharmacopoeia of Ukraine, the chemical composition, and pharmacological properties of species of the genus Alchemilla L. are insufficiently studied, therefore, it is advisable to study the chemical composition of the most common species of flora of Ukraine for their introduction into medical and pharmaceutical practice. The aim of the work was to study the component composition and quantitative content of essential oils of flowers and leaves of some species of the genus Alchemilla L. Materials and methods. Flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus. harvested in the Ivano-Frankivsk region in 2020 - 2021. The component composition and quantitative content of essential oils were determined by chromato-mass spectrometric method. The compounds were identified by comparing the obtained mass spectra of the chromatographic peak with the mass spectra of the reference compounds and based on comparison with the spectra of the database. Quantitative determination of the content of substances in the raw material was performed in comparison with a standard sample of menthol. Results. The essential oil of flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus were obtained. It was found that the highest content of essential oils was characterized by Alchemilla. flabellata Bus. flowers (16884.6 mg / kg), and the least essential oil was contained in the leaves of Alchemilla Phegophila Juz. (4895.5 mg / kg). As a result of studying the component composition of essential oils of flowers and leaves of Alchemilla flabellata Bus., Alchemilla Phegophila Juz. and Alchemilla subrenata Bus. 48, 51 and 47 compounds were identified, of which 44, 48 and 43 were identified, respectively. 31 components of essential oil were common in the studied samples of raw materials. Conclusions. Due to the component composition of the essential oil of flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus., as well as considering the known pharmacological activity of its components, it is advisable to conduct further pharmacological studies of raw materials of the genus Alchemilla L. to study their antibacterial, anti-inflammatory and antitumor properties

Jasminum L. of the Oleaceae family is a genus of plants cultivated for its aromatic flowers, which are a source of essential oil (EO). In temperate countries, jasmine, or pseudo jasmine, is often called Philadelphus coronarius L. of the Hydrangeáceae or Philadelphaceae family due to its similar fragrance. The aim. The aim of the study was to compare the component composition of volatile compounds of hydrodistillates and hexane extracts from flowers of Philadelphus coronarius L. and Jasminum officinale L.. Materials and Methods. Hydrodistillates obtained from dried flowers of J. officinale and from dried and fresh flowers of P. coronarius, as well as hexane extracts from similar raw materials, were analyzed by GC-MS. Research results. 109 compounds were identified. It was found that in the EO of J. officinale, obtained by hydrodistillation, the terpenoid content is 90.31 %, while in the hexane extract of the same raw material, the terpenoid content is only 36.24 %. In the EO of P. coronarius, obtained by hydrodistillation of dry flowers, the terpenoid content is 50.04 %, and from fresh flowers – 45.13 %. In the hexane extract of dry flowers of P. coronarius, the terpenoid content is only 14.63 %, while in the extract of fresh flowers – 52.55 %. In the EO of J. officinale obtained by hydrodistillation, the dominant components are (E)-geranyl linalool (12.86 %), linalool (10.72 %), (Z)-3-hexen-1-ol benzoate (7.82 %), α-farnesene (7.72 %), D-limonene (6.43 %), methyl anthranilate (5.9 %), (Z)-9-tricosene (4.15 %). In the EO obtained by hydrodistillation from dried flowers of P. coronarius, the dominant components are (1R)-(-)-myrtenal (12.73 %), myrtanal (11.09 %), pentadecanal (9.42 %), tricosane (8.33 %), (Z)-jasmone (7.09 %). In the EO, it is obtained by hydrodistillation from fresh P. coronarius flowers, the dominant components are: nerolidol (19.42 %), ethyl palmitate (19.13 %), methyl 2-methylpalmitate (16.44 %), myrtanal (9.91 %), pentadecanal (5.28 %), (Z)-jasmone (2.72 %). Conclusions. The conducted studies identified the main differences in volatile compounds in distillates and hexane extracts of P. coronarius and J. officinale. A total of 109 compounds were identified in the objects, and the dominant components were established. During the drying process of P. coronarius flowers, the composition of the EO significantly changes. Only hexane extracts from dried flowers of J. officinale and P. coronarius contain triterpene squalene in significant amounts (13.96 % and 6.72 %). Common to the hexane extracts of the studied objects are aromatic compounds: benzyl alcohol, 2,4-di-tert-butylphenol; aliphatic compounds: 2,4-dimethyl-heptane, octanal, decanal