The most promising plant from the genus Agrimony (Agrimonia L.) of the Rosaceae family for use in medical practice is Agrimonia eupatoria L. Phytochemical and pharmacological research in Agrimonia eupatoria L. herb extract, obtained with using 40% ethanol solution as an extractant were carried out. A total of 11 free and 17 bound monosaccharides, 17 amino acids were found in the studied extract, 9 of which are essential. Gallic and ellagic acids, gallocatechin, epigallocatechin, catechin, epicatechin, and epicatechin gallate were identified in the extract of A. eupatoria by the HPLC method; as well as hydroxycinnamic acids: hydroxyphenylacetate, caffeic, syringic, p-coumaric, ferulic, sinapic, cinnamic and quinic acid; flavonoids: quercetin-3-D-glucoside (isoquercitrin), neohesperidin, naringenin, luteolin were found, and their quantitative content was determined, as well by spectrophotometric methods. The herb extract of A. eupatoria belongs to practically non-toxic substances and has pronounced anti-inflammatory (at a dose of 10.0 mg/kg anti-exudative activity reached a maximum in 5 h (88.17%)) and hepatoprotective activity (at a dose of 25 mg/kg it reduce AlAt level by 1.1 and 1.2 times, respectively; AsAt by 1.2 and 1.1 times, respectively), reduces the level of lipid peroxidation and stabilizes the membrane structures of liver cells. Thus, the herb extract of A. eupatoria is a promising substance for the creation of phytomedicines with anti-inflammatory and hepatoprotective activity.

The aim. Extracts of cranberry large leaves have prospects when used to correct insulin-resistant conditions. There­fore, to create new drugs based on cranberry leaves, you need to develop instructions for cultivating the plant, harvesting raw materials and methods of quality control. Therefore, the aim of the research was to determine the pa­rameters of standardization of large cranberry leaves (Oxycoccus macrocarpus (Ait.) Pursh) and swamp cranberries (Oxycoccus palustris Pers.). Materials and methods. Macro- and microscopic studies of raw materials were performed according to the method of SPhU 2.8.23 “Microscopic examination of medicinal plant raw materials”. Macroscopic examinations were per­formed using a magnifying glass and MBS-9 binocular microscope. Identification of basic substances was performed by TLC, testing and quantification of flavonoids according to SPhU methods. Results. The morphological and anatomical features of the leaves of common cranberry and large cranberry were determined. The general features of the structure of stems and leaves species and different differences for each species are were revealed. TLC identification of the main BAS of raw materials was developed and standardization parameters were determined. Conclusions. The parameters of cranberry leaf standardization are determined by the following indicators: mac­ro-and microscopic features, TLC identification of the main BAS raw materials (hyperoside, rutin and caffeic acid), impurities (not more than 2 %), brown stems not more than 5 %, weight loss during drying (not more than 10 %), total ash (not more than 7 %) and not less than 1 % of flavonoids, in terms of hyperoside

Background/Objectives: The Canadian goldenrod (Solidago canadensis L.) is one of the most widespread species of the genus Solidago from the Asteraceae family. It has a rich composition of biologically active compounds and is traditionally used to address kidney, urinary tract, and liver diseases. Previously, it was proven that the S. canadensis extract obtained with a 40% ethanol solution had the most promising anti-inflammatory and hepatoprotective activity. Therefore, this extract was selected for the further formulation of amino acid preparations and 3D-printed dosage forms. The aims of the present study were to investigate the chemical composition, toxicity, and antimicrobial, anti-inflammatory, and hepatoprotective activity of S. canadensis dry extract, its amino acid preparations, and 3D-printed dosage forms. Results: A total of 18 phenolic compounds and 14 amino acids were determined in the extracts. The S. canadensis herb extracts were verified to be practically non-toxic preparations (toxicity class V, LD₅₀ > 5000 mg/kg). They also showed moderate antimicrobial activity against Staphylococcus aureus, Enterococcus faecalis, and β-hemolytic Streptococcus pyogenes. The most pronounced hepatoprotective activity was observed with S. canadensis herb extract and its amino acid preparations with phenylalanine, alanine, and lysine at a dose of 25 mg/kg body weight. The most pronounced anti-inflammatory activity was found with S. canadensis herb extract and its preparation with arginine. According to the calculated docking score array and the analysis of binding modes in the active sites of COX-1 and COX-2, the flavonoid fraction and caffeic acid in the S. canadensis extracts presented moderate inhibitory activity. Conclusions: The development of innovative 3D-printed oral dosage forms represents a promising strategy to formulate dietary supplements or pharmaceutical preparations for these herbal extracts.

The genus Alchemilla L. has about 1000 species in the world flora, of which 35 grow in Ukraine. The most common in Ukraine are A. flabellata Bus., A. phegophila Juz. and A. subrenata Bus. Despite the widespread distribution of species of the genus Alchemilla L. and the publication of a monograph on the herb of the collective species - Herba Alchemillae (Alchemiilla vulgaris L. sensu latiore) in the State Pharmacopoeia of Ukraine, the chemical composition, and pharmacological properties of species of the genus Alchemilla L. are insufficiently studied, therefore, it is advisable to study the chemical composition of the most common species of flora of Ukraine for their introduction into medical and pharmaceutical practice. The aim of the work was to study the component composition and quantitative content of essential oils of flowers and leaves of some species of the genus Alchemilla L. Materials and methods. Flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus. harvested in the Ivano-Frankivsk region in 2020 - 2021. The component composition and quantitative content of essential oils were determined by chromato-mass spectrometric method. The compounds were identified by comparing the obtained mass spectra of the chromatographic peak with the mass spectra of the reference compounds and based on comparison with the spectra of the database. Quantitative determination of the content of substances in the raw material was performed in comparison with a standard sample of menthol. Results. The essential oil of flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus were obtained. It was found that the highest content of essential oils was characterized by Alchemilla. flabellata Bus. flowers (16884.6 mg / kg), and the least essential oil was contained in the leaves of Alchemilla Phegophila Juz. (4895.5 mg / kg). As a result of studying the component composition of essential oils of flowers and leaves of Alchemilla flabellata Bus., Alchemilla Phegophila Juz. and Alchemilla subrenata Bus. 48, 51 and 47 compounds were identified, of which 44, 48 and 43 were identified, respectively. 31 components of essential oil were common in the studied samples of raw materials. Conclusions. Due to the component composition of the essential oil of flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus., as well as considering the known pharmacological activity of its components, it is advisable to conduct further pharmacological studies of raw materials of the genus Alchemilla L. to study their antibacterial, anti-inflammatory and antitumor properties

The liver is an organ with several important biofunctions, for which there are very few effective and safe preparations that promote the functioning, protection, and regeneration of cells. Sufficiently safe preparations with hepatoprotective activity can be found in plants. The aim of our study was to investigate the chemical composition of an extract made from American cranberry (Oxycoccus macrocarpus (Ait.) Pursh) leaves and its amino-acids preparations as well as their possible hepatoprotective activity. Using the UPLC-MS/MS method, we identified 19 phenolic compounds (8 flavonoids (flavones and flavonols), 4 anthocyanins, 3 hydroxycinnamic acids, and 2 catechins). The prophylactic and therapeutic administration of the American cranberry-leaves extracts led to a decrease in the lipid-peroxidation process during a study of tetrachloromethane acute toxic damage in the liver of rats. As a result of animal studies, the most effective hepatoprotective activity was found in the extract preparations with valine and arginine.

Анотація. Технологічні параметри Alchemilla s ubcrenata B us . досліджено та визначено втрату маси при сушці, питому, об’ємну та насипну масу, пористість та вільний об’єм лікарської рослинної сировини. Експериментально встановлено оптимальні умови отримання екстрактів досліджуваної сировини (розмір частинок, співвідношення сировина – екстрагент, час настоювання, частота екстрагування).