Physical exercise strongly affects human metabolism and causes biochemical changes. This study aimed to investigate the relationship between routine plasma biomarker levels and recovery efficiency in soccer players during an entire competitive match season. The players participating in the study were divided into a midfielder/defender group (seven midfielders and seven defenders) and a goalie/substitute group (six persons—goalkeepers and players with a short cumulative match-time). The fasting capillary blood samples were taken 17–24 h after each competitive match. The blood plasma was used to determine the creatinine, urea, alkaline phosphatase, creatine kinase, lactate dehydrogenase, aspartate and alanine aminotransferase, iron and magnesium levels of the athletes. The levels of (AST) (aspartate aminotransferase), (ALT) (alanine aminotransferase) and (Cr) creatinine were higher in the midfielder/defender group than in the control group, but only AST and Cr significantly varied over time (AST decreased, and Cr increased with time). The (LDH) (lactate dehydrogenase) activity and urea level were significantly lower in the midfielder/defender group than in the goalie/substitute group, and it significantly varied over time (LDH decreased, and urea increased with time). No differences in the (CK) creatine kinase and (ALP) alkaline phosphatase activities between the groups was found, although CK increased significantly with time in the midfielder/defender group (particularly midfielders in the spring round). In midfielders, the AST activity and the iron level were significantly lower in the spring than in the autumn round. On the contrary, ALT, CK, urea and magnesium levels were significantly higher in the spring than in autumn round. A long-term measurement of biochemical parameters in elite soccer players indicated that AST, CK, LDH and creatinine levels, when analyzed together, could constitute a useful set of markers for monitoring recovery periods.

(1) Background: This study aimed to assess whether older adults exhibit greater discrepancies between intended and actual motor unit recruitment, which could affect the quality of muscle activation and potentially increase the risk of falls. (2) Methods: Forty-eight physically active older women were assessed (65 ± 6 years, 164 ± 6 cm, and 76 ± 7 kg). The bioelectrical activity (EMG) of the vastus lateralis oblique (VLO) and vastus medialis oblique (VMO) muscles were assessed during isometric testing with the knee joint bent to 75 degrees. The participants were instructed to press against a stable bar for 5 s at a specific percentage of their perceived force level (at 15%, 30%, and 60% of MVC) when the EMG activity was recorded. Balance was assessed using a stabilometric platform in a standing position. (3) Results: In all three thresholds, the bioelectrical activity of the VLO and VMO muscles significantly deviated from what was expected under the assumption of a nearly linear relationship between muscle force and bioelectrical activity. In each of the three thresholds, it did not exceed 10% MVC and significantly differed only between the 15% and 60% MVC thresholds. No significant differences were found between the dominant and non-dominant sides. A significant relationship was observed between the sway area (Area 95%) and the activity of the non-dominant limb VLO muscle. (4) Conclusions: Our results suggest that older adults experience deficits in muscle activation perception, leading to discrepancies between intended and actual muscle engagement, which may affect functional task performance and potentially increase fall risk.

Introduction: Basketball performance requires not only intermittent high-intensity movements—such as sprinting, jumping, and rapid directional changes—but also rapid decision-making under cognitive and psychological stress. Transcranial direct current stimulation (tDCS) has emerged as a potential modality to enhance both physical and mental performance due to its capacity to modulate cortical excitability and promote synaptic plasticity. Although the broader literature suggests that tDCS can benefit motor performance and endurance across various sports, its specific impact on basketball remains underexplored. Methods: This scoping review aimed to summarize current evidence on the effects of tDCS in basketball. A comprehensive literature search was conducted across databases including PubMed/Medline, Google Scholar, and Cochrane, identifying studies published between January 2008 and February 2025. Only clinical trials investigating tDCS interventions in basketball players were included. Eleven articles met the inclusion criteria and were synthesized narratively, with a focus on stimulation parameters (site, duration, intensity) and performance outcomes (shooting accuracy, dribbling, sprinting, decision-making, fatigue). Results: The reviewed studies indicated that tDCS—particularly when applied over the motor cortex—was associated with moderate improvements in shooting accuracy, dribbling time, repeated-sprint performance, and decision-making under fatigue. Some studies reported delayed rather than immediate benefits, suggesting that tDCS may prime neural networks for enhanced learning and retention. However, not all findings were consistent; certain interventions produced minimal or no significant effects, especially regarding subjective mental fatigue and cognitive workload. The variability in electrode placements and stimulation protocols highlights the need for methodological standardization. Conclusions: Current evidence partially supports the potential of tDCS to improve specific performance domains in basketball, particularly in skill acquisition, neuromuscular efficiency, and decision-making. Nevertheless, the findings are limited by small sample sizes, heterogeneous protocols, and a lack of long-term follow-up. Future research should prioritize larger, multisite studies with standardized tDCS parameters and ecologically valid outcome measures to confirm the efficacy and practical relevance of tDCS in competitive basketball settings.

According to the World Health Organization (WHO), a healthy lifestyle is defined as a way of living that lowers the risk of becoming seriously ill or dying prematurely. Physical activity, as a well-known contributor to overall health, plays a vital role in supporting such a lifestyle. Exercise induces complex molecular responses that mediate both acute metabolic stress and long-term physiological adaptations. FGF21 (fibroblast growth factor 21) and GDF15 (growth differentiation factor 15) are recognized as metabolic stress markers, while their receptors play critical roles in cellular signaling. However, the differential gene expression patterns of these molecules in trained and untrained individuals following exhaustive exercise remain poorly understood. This study aimed to examine the transcriptional and protein-level responses in trained and untrained individuals performed a treadmill maximal exercise test to voluntary exhaustion. Blood samples were collected at six time points (pre-exercise, immediately post-exercise, and 0.5 h, 6 h, 24 h, and 48 h post-exercise). Gene expression of FGF21, GDF15, FGFR1 (fibroblast growth factor receptors), FGFR3, FGFR4, KLB (β-klotho), and GFRAL (glial cell line-derived neurotrophic factor receptor alpha-like) was analyzed using RT-qPCR, while plasma protein levels of FGF21 and GDF15 were quantified via ELISA. The results obtained were statistically analyzed by using Shapiro–Wilk, Mann–Whitney U, and Wilcoxon tests in Statistica 13 software. Untrained individuals demonstrated significant post-exercise upregulation of FGFR3, FGFR4, KLB, and GFRAL. FGF21 and GDF15 protein levels were consistently lower in trained individuals (p < 0.01), with no significant correlations between gene and protein expression. Trained individuals showed more stable expression of genes, while untrained individuals exhibited transient upregulation of genes after exercise.

Objectives: Rupture of the anterior cruciate ligament (ACL) is a severe injury common in sports. It also has a high rate of re-injury. The aim of this work was to assess hamstring muscle fatigue in active football players after ACL reconstruction who were cleared to play and to determine symmetry between the lateral and medial hamstring muscles. Methods: In professional football players post ACL reconstruction (n = 25) and non-injured players (n = 26), the bioelectrical activity of the medial (biceps femoris—BF) and lateral (semimembranosus and semitendinosus—SEM) hamstring muscles was measured during 60 s of isometric contraction. The fatigue variables were calculated using the Continuous Wavelet Transform (CWT) tool. Results: The football players following ACL reconstruction demonstrated significant asymmetry in fatigue of the lateral and medial hamstring muscles, with greater fatigue in the SEM compared to the BF muscle. Moreover, in those after reconstruction, the changes are more pronounced, with higher muscle fatigue in both limbs (they have lower MDF than non-injured players) and more severe SEM muscle insufficiency (noted in both limbs but with greater intensity in the non-operated one). Conclusions: The higher SEM muscle fatigue observed in this study influenced the lateral-to-medial activation ratio within the hamstring muscle, which may be a probable cause of this muscle’s insufficiency in laterally stabilizing the knee in the frontal and transverse plane. Furthermore, the hamstring muscles after reconstruction were more fatigued in both limbs, which may be another risk factor for ACL graft rupture. Therefore, increased fatigue in specific hamstring muscles may indicate the direction in which knee stabilization is compromised due to ACL overload. A muscle that becomes fatigued and inefficient more quickly also becomes ineffective in performing its function sooner, which can lead to increased overloading forces acting on the ACL graft.

Wydawnictwo Naukowe Uniwersytetu Szczecińskiego