This page has only limited features, please log in for full access.

Dr. Carlos García
Instituto de Biotecnología de León (INBIOTEC), León, Spain; and Universidad de León, Campus Vegazana S-N, E-24071 León, Spain

Basic Info

Basic Info is private.

Research Keywords & Expertise

0 Biotechnology
0 Genetic Engineering
0 Molecular Biology
0 OMICS
0 Fungal secondary metabolism

Fingerprints

OMICS
Biotechnology
Fungal secondary metabolism
Molecular Biology

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Review
Published: 12 August 2021 in Sustainable Chemistry
Reads 0
Downloads 0

Polymers are essential materials in our daily life. The synthesis of value-added polymers is mainly performed from fossil fuel-derived monomers. However, the adoption of the circular economy model based on the bioeconomy will reduce the dependence on fossil fuels. In this context, biorefineries have emerged to convert biomass into bioenergy and produce high value-added products, including molecules that can be further used as building blocks for the synthesis of biopolymers and bioplastics. The achievement of catalytic systems able to polymerize the natural monomer counterparts, such as terpenes or terpenoids, is still a challenge in the development of polymers with good mechanical, thermal, and chemical properties. This review describes the most common types of bioplastics and biopolymers and focuses specifically on the polymerization of terpenes and terpenoids, which represent a source of promising monomers to create bio-based polymers and copolymers.

ACS Style

Marta. E. G. Mosquera; Gerardo Jiménez; Vanessa Tabernero; Joan Vinueza-Vaca; Carlos García-Estrada; Katarina Kosalková; Alberto Sola-Landa; Belén Monje; Carolina Acosta; Rafael Alonso; Miguel Ángel Valera. Terpenes and Terpenoids: Building Blocks to Produce Biopolymers. Sustainable Chemistry 2021, 2, 467 -492.

AMA Style

Marta. E. G. Mosquera, Gerardo Jiménez, Vanessa Tabernero, Joan Vinueza-Vaca, Carlos García-Estrada, Katarina Kosalková, Alberto Sola-Landa, Belén Monje, Carolina Acosta, Rafael Alonso, Miguel Ángel Valera. Terpenes and Terpenoids: Building Blocks to Produce Biopolymers. Sustainable Chemistry. 2021; 2 (3):467-492.

Chicago/Turabian Style

Marta. E. G. Mosquera; Gerardo Jiménez; Vanessa Tabernero; Joan Vinueza-Vaca; Carlos García-Estrada; Katarina Kosalková; Alberto Sola-Landa; Belén Monje; Carolina Acosta; Rafael Alonso; Miguel Ángel Valera. 2021. "Terpenes and Terpenoids: Building Blocks to Produce Biopolymers." Sustainable Chemistry 2, no. 3: 467-492.

Review
Published: 27 May 2021 in Biology
Reads 0
Downloads 0

Diseases caused by trypanosomatids (Sleeping sickness, Chagas disease, and leishmaniasis) are a serious public health concern in low-income endemic countries. These diseases are produced by single-celled parasites with a diploid genome (although aneuploidy is frequent) organized in pairs of non-condensable chromosomes. To explain the way they reproduce through the analysis of natural populations, the theory of strict clonal propagation of these microorganisms was taken as a rule at the beginning of the studies, since it partially justified their genomic stability. However, numerous experimental works provide evidence of sexual reproduction, thus explaining certain naturally occurring events that link the number of meiosis per mitosis and the frequency of mating. Recent techniques have demonstrated genetic exchange between individuals of the same species under laboratory conditions, as well as the expression of meiosis specific genes. The current debate focuses on the frequency of genomic recombination events and its impact on the natural parasite population structure. This paper reviews the results and techniques used to demonstrate the existence of sex in trypanosomatids, the inheritance of kinetoplast DNA (maxi- and minicircles), the impact of genetic exchange in these parasites, and how it can contribute to the phenotypic diversity of natural populations.

ACS Style

Camino Gutiérrez-Corbo; Bárbara Domínguez-Asenjo; María Martínez-Valladares; Yolanda Pérez-Pertejo; Carlos García-Estrada; Rafael Balaña-Fouce; Rosa Reguera. Reproduction in Trypanosomatids: Past and Present. Biology 2021, 10, 471 .

AMA Style

Camino Gutiérrez-Corbo, Bárbara Domínguez-Asenjo, María Martínez-Valladares, Yolanda Pérez-Pertejo, Carlos García-Estrada, Rafael Balaña-Fouce, Rosa Reguera. Reproduction in Trypanosomatids: Past and Present. Biology. 2021; 10 (6):471.

Chicago/Turabian Style

Camino Gutiérrez-Corbo; Bárbara Domínguez-Asenjo; María Martínez-Valladares; Yolanda Pérez-Pertejo; Carlos García-Estrada; Rafael Balaña-Fouce; Rosa Reguera. 2021. "Reproduction in Trypanosomatids: Past and Present." Biology 10, no. 6: 471.

Protocol
Published: 12 May 2021 in Methods in Molecular Biology
Reads 0
Downloads 0

Penicillium chrysogenum, recently re-identified as Penicillium rubens, is the microorganism used for the industrial production of penicillin. This filamentous fungus (mold) probably represents the best example of adaptation of a microorganism to industrial production conditions and therefore, it can be considered as a model organism for the study of primary and secondary metabolism under a highly stressful environment. In this regard, biosynthesis and production of benzylpenicillin can be used as an interesting phenotypic trait for those studies. In this chapter, we describe P. chrysogenum culture procedures for the production of benzylpenicillin and the process of antibiotic quantitation either by bioassay or by high-performance liquid chromatography (HPLC).

ACS Style

Katarina Kosalková; Isabel-Clara Sánchez-Orejas; Laura Cueto; Carlos García-Estrada. Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC. Methods in Molecular Biology 2021, 2296, 195 -207.

AMA Style

Katarina Kosalková, Isabel-Clara Sánchez-Orejas, Laura Cueto, Carlos García-Estrada. Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC. Methods in Molecular Biology. 2021; 2296 ():195-207.

Chicago/Turabian Style

Katarina Kosalková; Isabel-Clara Sánchez-Orejas; Laura Cueto; Carlos García-Estrada. 2021. "Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC." Methods in Molecular Biology 2296, no. : 195-207.

Review
Published: 26 June 2020 in Genes
Reads 0
Downloads 0

Penicillin biosynthesis by Penicillium chrysogenum is one of the best-characterized biological processes from the genetic, molecular, biochemical, and subcellular points of view. Several omics studies have been carried out in this filamentous fungus during the last decade, which have contributed to gathering a deep knowledge about the molecular mechanisms underlying improved productivity in industrial strains. The information provided by these studies is extremely useful for enhancing the production of penicillin or other bioactive secondary metabolites by means of Biotechnology or Synthetic Biology.

ACS Style

Carlos García-Estrada; Juan Martín; Laura Cueto; Carlos Barreiro. Omics Approaches Applied to Penicillium chrysogenum and Penicillin Production: Revealing the Secrets of Improved Productivity. Genes 2020, 11, 712 .

AMA Style

Carlos García-Estrada, Juan Martín, Laura Cueto, Carlos Barreiro. Omics Approaches Applied to Penicillium chrysogenum and Penicillin Production: Revealing the Secrets of Improved Productivity. Genes. 2020; 11 (6):712.

Chicago/Turabian Style

Carlos García-Estrada; Juan Martín; Laura Cueto; Carlos Barreiro. 2020. "Omics Approaches Applied to Penicillium chrysogenum and Penicillin Production: Revealing the Secrets of Improved Productivity." Genes 11, no. 6: 712.

Review
Published: 31 March 2020 in Marine Drugs
Reads 0
Downloads 0

Neglected Tropical Diseases (NTD) represent a serious threat to humans, especially for those living in poor or developing countries. Almost one-sixth of the world population is at risk of suffering from these diseases and many thousands die because of NTDs, to which we should add the sanitary, labor and social issues that hinder the economic development of these countries. Protozoan-borne diseases are responsible for more than one million deaths every year. Visceral leishmaniasis, Chagas disease or sleeping sickness are among the most lethal NTDs. Despite not being considered an NTD by the World Health Organization (WHO), malaria must be added to this sinister group. Malaria, caused by the apicomplexan parasite Plasmodium falciparum, is responsible for thousands of deaths each year. The treatment of this disease has been losing effectiveness year after year. Many of the medicines currently in use are obsolete due to their gradual loss of efficacy, their intrinsic toxicity and the emergence of drug resistance or a lack of adherence to treatment. Therefore, there is an urgent and global need for new drugs. Despite this, the scant interest shown by most of the stakeholders involved in the pharmaceutical industry makes our present therapeutic arsenal scarce, and until recently, the search for new drugs has not been seriously addressed. The sources of new drugs for these and other pathologies include natural products, synthetic molecules or repurposing drugs. The most frequent sources of natural products are microorganisms, e.g., bacteria, fungi, yeasts, algae and plants, which are able to synthesize many drugs that are currently in use (e.g. antimicrobials, antitumor, immunosuppressants, etc.). The marine environment is another well-established source of bioactive natural products, with recent applications against parasites, bacteria and other pathogens which affect humans and animals. Drug discovery techniques have rapidly advanced since the beginning of the millennium. The combination of novel techniques that include the genetic modification of pathogens, bioimaging and robotics has given rise to the standardization of High-Performance Screening platforms in the discovery of drugs. These advancements have accelerated the discovery of new chemical entities with antiparasitic effects. This review presents critical updates regarding the use of High-Throughput Screening (HTS) in the discovery of drugs for NTDs transmitted by protozoa, including malaria, and its application in the discovery of new drugs of marine origin.

ACS Style

María Álvarez-Bardón; Yolanda Pérez-Pertejo; César Ordóñez; Daniel Sepúlveda-Crespo; Nestor M. Carballeira; Babu L. Tekwani; Sankaranarayanan Murugesan; Maria Martinez-Valladares; Carlos García-Estrada; Rosa M. Reguera; Rafael Balaña-Fouce. Screening Marine Natural Products for New Drug Leads against Trypanosomatids and Malaria. Marine Drugs 2020, 18, 187 .

AMA Style

María Álvarez-Bardón, Yolanda Pérez-Pertejo, César Ordóñez, Daniel Sepúlveda-Crespo, Nestor M. Carballeira, Babu L. Tekwani, Sankaranarayanan Murugesan, Maria Martinez-Valladares, Carlos García-Estrada, Rosa M. Reguera, Rafael Balaña-Fouce. Screening Marine Natural Products for New Drug Leads against Trypanosomatids and Malaria. Marine Drugs. 2020; 18 (4):187.

Chicago/Turabian Style

María Álvarez-Bardón; Yolanda Pérez-Pertejo; César Ordóñez; Daniel Sepúlveda-Crespo; Nestor M. Carballeira; Babu L. Tekwani; Sankaranarayanan Murugesan; Maria Martinez-Valladares; Carlos García-Estrada; Rosa M. Reguera; Rafael Balaña-Fouce. 2020. "Screening Marine Natural Products for New Drug Leads against Trypanosomatids and Malaria." Marine Drugs 18, no. 4: 187.

Review
Published: 19 November 2019 in Current Medicinal Chemistry
Reads 0
Downloads 0

DNA topoisomerases (Top) are a group of isomerase enzymes responsible for controlling the topological problems caused by DNA double helix in the cell during the processes of replication, transcription and recombination. Interestingly, these enzymes have been known since long to be key molecular machines in several cellular processes through overwinding or underwinding of DNA in all living organisms. Leishmania, a trypanosomatid parasite responsible for causing fatal diseases mostly in impoverished populations of low-income countries, has a set of six classes of Top enzymes. These are placed in the nucleus and the single mitochondrion and can be deadly targets of suitable drugs. Given the fact that there are clear differences in structure and expression between parasite and host enzymes, numerous studies have reported the therapeutic potential of Top inhibitors as antileishmanial drugs. In this regard, numerous compounds have been described as Top type IB and Top type II inhibitors in Leishmania parasites, such as camptothecin derivatives, indenoisoquinolines, indeno-1,5- naphthyridines, fluoroquinolones, anthracyclines and podophyllotoxins. The aim of this review is to highlight several facts about Top and Top inhibitors as potential antileishmanial drugs, which may represent a promising strategy for the control of this disease of public health importance.

ACS Style

Rosa M. Reguera; Ehab Kotb Elmahallawy; Carlos Garcia-Estrada; Rubén Carbajo-Andrés; Rafael Balaña-Fouce. DNA Topoisomerases of Leishmania Parasites; Druggable Targets for Drug Discovery. Current Medicinal Chemistry 2019, 26, 5900 -5923.

AMA Style

Rosa M. Reguera, Ehab Kotb Elmahallawy, Carlos Garcia-Estrada, Rubén Carbajo-Andrés, Rafael Balaña-Fouce. DNA Topoisomerases of Leishmania Parasites; Druggable Targets for Drug Discovery. Current Medicinal Chemistry. 2019; 26 (32):5900-5923.

Chicago/Turabian Style

Rosa M. Reguera; Ehab Kotb Elmahallawy; Carlos Garcia-Estrada; Rubén Carbajo-Andrés; Rafael Balaña-Fouce. 2019. "DNA Topoisomerases of Leishmania Parasites; Druggable Targets for Drug Discovery." Current Medicinal Chemistry 26, no. 32: 5900-5923.

Protocol
Published: 15 August 2018 in Methods in Molecular Biology
Reads 0
Downloads 0

Xanthophyllomyces dendrorhous, a heterobasidiomycetous yeast that represents the teleomorphic state of Phaffia rhodozyma, is used as a natural source of several carotenoids, such as the xanthophyll astaxanthin. Here, we describe the culture procedure for the production of carotenoids in X. dendrorhous and a simple and rapid analytical method for the optimized extraction and HPLC determination of intracellular β-carotene, astaxanthin, canthaxanthin, and zeaxanthin.

ACS Style

Carlos García-Estrada; Katarina Kosalkova; Isabel Clara Sánchez Y Orejas. Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous. Methods in Molecular Biology 2018, 1852, 283 -295.

AMA Style

Carlos García-Estrada, Katarina Kosalkova, Isabel Clara Sánchez Y Orejas. Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous. Methods in Molecular Biology. 2018; 1852 ():283-295.

Chicago/Turabian Style

Carlos García-Estrada; Katarina Kosalkova; Isabel Clara Sánchez Y Orejas. 2018. "Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous." Methods in Molecular Biology 1852, no. : 283-295.

Journal article
Published: 06 August 2018 in Journal of Proteomics
Reads 0
Downloads 0

Biosynthesis of benzylpenicillin in filamentous fungi (e.g. Penicillium chrysogenum - renamed as Penicillium rubens- and Aspergillus nidulans) depends on the addition of CoA-activated forms of phenylacetic acid to isopenicillin N. Phenylacetic acid is also detoxified by means of the homogentisate pathway, which begins with the hydroxylation of phenylacetic acid to 2-hydroxyphenylacetate in a reaction catalysed by the pahA-encoded phenylacetate hydroxylase. This catabolic step has been tested in three different penicillin-producing strains of P. rubens (P. notatum, P. chrysogenum NRRL 1951 and P. chrysogenum Wisconsin 54–1255) in the presence of sucrose and lactose as non-repressing carbon sources. P. chrysogenum Wisconsin 54–1255 was able to accumulate 2-hydroxyphenylacetate at late culture times. Analysis of the P. rubens genome showed the presence of several PahA homologs, but only Pc16g01770 was transcribed under penicillin production conditions. Gene knock-down experiments indicated that the protein encoded by Pc16g01770 seems to have residual activity in phenylacetic acid degradation, this catabolic activity having no effect on benzylpenicillin biosynthesis. Proteome-wide analysis of the Wisconsin 54–1255 strain in response to phenylacetic acid revealed that this molecule has a positive effect on some proteins directly related to the benzylpenicillin biosynthetic pathway, the synthesis of amino acid precursors and other important metabolic processes. The adaptive response of Penicillium rubens to benzylpenicillin production conditions remains to be fully elucidated. This article provides important information about the molecular mechanisms interconnected with phenylacetate (benzylpenicillin side chain precursor) utilization and penicillin biosynthesis, and will contribute to the understanding of the complex physiology and adaptation mechanisms triggered by P. rubens (P. chrysogenum Wisconsin 54–1255) under benzylpenicillin production conditions.

ACS Style

Mohammad-Saeid Jami; Juan-Francisco Martín; Carlos Barreiro; Rebeca Domínguez-Santos; María-Fernanda Vasco-Cárdenas; María Pascual; Carlos García-Estrada. Catabolism of phenylacetic acid in Penicillium rubens. Proteome-wide analysis in response to the benzylpenicillin side chain precursor. Journal of Proteomics 2018, 187, 243 -259.

AMA Style

Mohammad-Saeid Jami, Juan-Francisco Martín, Carlos Barreiro, Rebeca Domínguez-Santos, María-Fernanda Vasco-Cárdenas, María Pascual, Carlos García-Estrada. Catabolism of phenylacetic acid in Penicillium rubens. Proteome-wide analysis in response to the benzylpenicillin side chain precursor. Journal of Proteomics. 2018; 187 ():243-259.

Chicago/Turabian Style

Mohammad-Saeid Jami; Juan-Francisco Martín; Carlos Barreiro; Rebeca Domínguez-Santos; María-Fernanda Vasco-Cárdenas; María Pascual; Carlos García-Estrada. 2018. "Catabolism of phenylacetic acid in Penicillium rubens. Proteome-wide analysis in response to the benzylpenicillin side chain precursor." Journal of Proteomics 187, no. : 243-259.

Review
Published: 19 June 2018 in Fermentation
Reads 0
Downloads 0

Transcription factors are key regulatory proteins in all living beings. In fungi, transcription factors include either broad-domain regulatory proteins that affect the expression of many genes involved in biosynthetic processes, or proteins encoded by cluster-associated (also called pathway-specific) regulatory genes. Belonging to the most interesting transcription factors in fungi are binuclear zinc finger proteins. In addition to the transcription factors in the strict sense, other proteins play a very important role in the expression of genes for primary and secondary metabolism in fungi, such as winged helix regulators, the LaeA protein and the velvet complex. LaeA appears to be involved in heterochromatin reorganization, and the velvet complex proteins, which are nuclear factors that associate with LaeA, also have a determining role in both differentiation (sporulation) and secondary metabolite biosynthesis. The genes involved in the biosynthesis of β-lactam antibiotics are well known and serve as an excellent model to understand the transcriptional control of genes involved in the biosynthesis of secondary metabolites. Interaction between different regulatory proteins in the promoter regions may represent a cross-talk regulation between different gene clusters.

ACS Style

Carlos García-Estrada; Rebeca Domínguez-Santos; Katarina Kosalková; Juan-Francisco Martín. Transcription Factors Controlling Primary and Secondary Metabolism in Filamentous Fungi: The β-Lactam Paradigm. Fermentation 2018, 4, 47 .

AMA Style

Carlos García-Estrada, Rebeca Domínguez-Santos, Katarina Kosalková, Juan-Francisco Martín. Transcription Factors Controlling Primary and Secondary Metabolism in Filamentous Fungi: The β-Lactam Paradigm. Fermentation. 2018; 4 (2):47.

Chicago/Turabian Style

Carlos García-Estrada; Rebeca Domínguez-Santos; Katarina Kosalková; Juan-Francisco Martín. 2018. "Transcription Factors Controlling Primary and Secondary Metabolism in Filamentous Fungi: The β-Lactam Paradigm." Fermentation 4, no. 2: 47.

Review
Published: 30 July 2017 in Journal of Fungi
Reads 0
Downloads 0

Carotenoids are organic lipophilic yellow to orange and reddish pigments of terpenoid nature that are usually composed of eight isoprene units. This group of secondary metabolites includes carotenes and xanthophylls, which can be naturally obtained from photosynthetic organisms, some fungi, and bacteria. One of the microorganisms able to synthesise carotenoids is the heterobasidiomycetous yeast Xanthophyllomyces dendrorhous, which represents the teleomorphic state of Phaffia rhodozyma, and is mainly used for the production of the xanthophyll astaxanthin. Upgraded knowledge on the biosynthetic pathway of the main carotenoids synthesised by X. dendrorhous, the biotechnology-based improvement of astaxanthin production, as well as the current omics approaches available in this yeast are reviewed in depth.

ACS Style

Jose L. Barredo; Carlos García-Estrada; Katarina Kosalkova; Carlos Barreiro. Biosynthesis of Astaxanthin as a Main Carotenoid in the Heterobasidiomycetous Yeast Xanthophyllomyces dendrorhous. Journal of Fungi 2017, 3, 44 .

AMA Style

Jose L. Barredo, Carlos García-Estrada, Katarina Kosalkova, Carlos Barreiro. Biosynthesis of Astaxanthin as a Main Carotenoid in the Heterobasidiomycetous Yeast Xanthophyllomyces dendrorhous. Journal of Fungi. 2017; 3 (3):44.

Chicago/Turabian Style

Jose L. Barredo; Carlos García-Estrada; Katarina Kosalkova; Carlos Barreiro. 2017. "Biosynthesis of Astaxanthin as a Main Carotenoid in the Heterobasidiomycetous Yeast Xanthophyllomyces dendrorhous." Journal of Fungi 3, no. 3: 44.

Original research article
Published: 20 June 2017 in Frontiers in Microbiology
Reads 0
Downloads 0

One study using the rumen simulation technique (RUSITEC) investigated the changes in the ruminal microbiota and anaerobic fermentation in response to the addition of different lipid supplements to a ruminant diet. A basal diet with no oil added was the control, and the treated diets were supplemented with sunflower oil (2%) only, or sunflower oil (2%) in combination with fish oil (1%) or algae oil (1%). Four fermentation units were used per treatment. RUSITEC fermenters were inoculated with rumen digesta. Substrate degradation, fermentation end-products (volatile fatty acids, lactate, gas, methane and ammonia), and microbial protein synthesis were determined. Fatty acid profiles and microbial community composition were evaluated in digesta samples. Numbers of representative bacterial species and microbial groups were determined using qPCR. Composition of the microbial community and diversity were based on T-RFLP spectra. The addition of oils had not effects on substrate degradation or microbial protein synthesis. Differences among diets in NDF degradation were not significant (P = 0.132), but the contrast comparing oil–supplemented diets with the control was significant (P = 0.039). Methane production was reduced (P < 0.05) with all oil supplements. Propionate production was increased when diets containing oil were fermented. Compared with the control, the addition of algae oil decreased the percentage C18:3 c9c12c15 in rumen digesta, and that of C18:2 c9t11 was increased when the control diet was supplemented with any oil. Marine oils decreased the hydrogenation of C18 unsaturated fatty acids. Microbial diversity was not affected by oil supplementation. Cluster analysis showed that diets with additional fish or algae oils formed a group separated from the sunflower oil diet. Supplementation with marine oils decreased the numbers of Butyrivibrio producers of stearic acid, and affected the numbers of protozoa, methanogens, Selenomonas ruminantium and Streptococcus bovis, but not total bacteria. In conclusion, there is a potential to manipulate the rumen fermentation and microbiota with the addition of sunflower, fish or algae oils to ruminant diets at appropriate concentrations. Specifically, supplementation of ruminant mixed rations with marine oils will reduce methane production, the acetate to propionate ratio and the fatty acid hydrogenation in the rumen.

ACS Style

Julio E. Vargas; Sonia Andrés; Timothy J. Snelling; Lorena López-Ferreras; David R. Yáñez-Ruíz; Carlos Garcia-Estrada; Secundino Lopez. Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile. Frontiers in Microbiology 2017, 8, 1124 .

AMA Style

Julio E. Vargas, Sonia Andrés, Timothy J. Snelling, Lorena López-Ferreras, David R. Yáñez-Ruíz, Carlos Garcia-Estrada, Secundino Lopez. Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile. Frontiers in Microbiology. 2017; 8 ():1124.

Chicago/Turabian Style

Julio E. Vargas; Sonia Andrés; Timothy J. Snelling; Lorena López-Ferreras; David R. Yáñez-Ruíz; Carlos Garcia-Estrada; Secundino Lopez. 2017. "Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile." Frontiers in Microbiology 8, no. : 1124.

Journal article
Published: 01 March 2017 in Journal of Proteomics
Reads 0
Downloads 0

Penicillin biosynthesis in Penicillium chrysogenum is one of the best characterized secondary metabolism processes. However, the mechanism by which penicillin is secreted still remains to be elucidated. Taking into account the role played by Ca and CPP in the secretory pathway and considering the positive effect that Ca exerts on penicillin production, the analysis of global protein changes produced after CPP/CaCl addition is very helpful to decipher the processes related to the biosynthesis and secretion of penicillin.

ACS Style

Rebeca Domínguez-Santos; Katarina Kosalková; Carlos García-Estrada; Carlos Barreiro; Ana Ibáñez; Alejandro Morales; Juan-Francisco Martín. Casein phosphopeptides and CaCl2 increase penicillin production and cause an increment in microbody/peroxisome proteins in Penicillium chrysogenum. Journal of Proteomics 2017, 156, 52 -62.

AMA Style

Rebeca Domínguez-Santos, Katarina Kosalková, Carlos García-Estrada, Carlos Barreiro, Ana Ibáñez, Alejandro Morales, Juan-Francisco Martín. Casein phosphopeptides and CaCl2 increase penicillin production and cause an increment in microbody/peroxisome proteins in Penicillium chrysogenum. Journal of Proteomics. 2017; 156 ():52-62.

Chicago/Turabian Style

Rebeca Domínguez-Santos; Katarina Kosalková; Carlos García-Estrada; Carlos Barreiro; Ana Ibáñez; Alejandro Morales; Juan-Francisco Martín. 2017. "Casein phosphopeptides and CaCl2 increase penicillin production and cause an increment in microbody/peroxisome proteins in Penicillium chrysogenum." Journal of Proteomics 156, no. : 52-62.

Book chapter
Published: 19 November 2016 in Agriculturally Important Microorganisms
Reads 0
Downloads 0

Beauveria bassiana is the most widely used biocontrol agent against many major arthropod pests. This ascomycetal fungus is able to produce infection structures and synthesize a cocktail of proteins, enzymes, organic acids, and bioactive secondary metabolites, which are responsible for the entomopathogenic activity and virulence. For commercial purposes, B. bassiana is usually formulated using conidia with different stabilizing agents. Various types of formulation include bait/solid, encapsulation, and emulsion. Commercialization and marketing strategies, including alternative marketing channels, such as earthworm compost and compost, along with the legal framework are addressed in this chapter.

ACS Style

Carlos García-Estrada; Enrique Cat; Irene Santamarta. Beauveria bassiana as Biocontrol Agent: Formulation and Commercialization for Pest Management. Agriculturally Important Microorganisms 2016, 81 -96.

AMA Style

Carlos García-Estrada, Enrique Cat, Irene Santamarta. Beauveria bassiana as Biocontrol Agent: Formulation and Commercialization for Pest Management. Agriculturally Important Microorganisms. 2016; ():81-96.

Chicago/Turabian Style

Carlos García-Estrada; Enrique Cat; Irene Santamarta. 2016. "Beauveria bassiana as Biocontrol Agent: Formulation and Commercialization for Pest Management." Agriculturally Important Microorganisms , no. : 81-96.

Mini review
Published: 23 August 2016 in Applied Microbiology and Biotechnology
Reads 0
Downloads 0

Ripening of blue-veined cheeses, such as the French Bleu and Roquefort, the Italian Gorgonzola, the English Stilton, the Danish Danablu or the Spanish Cabrales, Picón Bejes-Tresviso, and Valdeón, requires the growth and enzymatic activity of the mold Penicillium roqueforti, which is responsible for the characteristic texture, blue-green spots, and aroma of these types of cheeses. This filamentous fungus is able to synthesize different secondary metabolites, including andrastins, mycophenolic acid, and several mycotoxins, such as roquefortines C and D, PR-toxin and eremofortins, isofumigaclavines A and B, and festuclavine. This review provides a detailed description of the main secondary metabolites produced by P. roqueforti in blue cheese, giving a special emphasis to roquefortine, PR-toxin and mycophenolic acid, and their biosynthetic gene clusters and pathways. The knowledge of these clusters and secondary metabolism pathways, together with the ability of P. roqueforti to produce beneficial secondary metabolites, is of interest for commercial purposes.

ACS Style

Carlos García-Estrada; Juan-Francisco Martín. Biosynthetic gene clusters for relevant secondary metabolites produced by Penicillium roqueforti in blue cheeses. Applied Microbiology and Biotechnology 2016, 100, 8303 -8313.

AMA Style

Carlos García-Estrada, Juan-Francisco Martín. Biosynthetic gene clusters for relevant secondary metabolites produced by Penicillium roqueforti in blue cheeses. Applied Microbiology and Biotechnology. 2016; 100 (19):8303-8313.

Chicago/Turabian Style

Carlos García-Estrada; Juan-Francisco Martín. 2016. "Biosynthetic gene clusters for relevant secondary metabolites produced by Penicillium roqueforti in blue cheeses." Applied Microbiology and Biotechnology 100, no. 19: 8303-8313.

Review
Published: 01 August 2016 in Veterinary Parasitology
Reads 0
Downloads 0

Canine leishmaniasis (CanL) is a parasite-borne disease mainly induced by Leishmania infantum in the Old World and Leishmania chagasi (infantum) in the New World. CanL is a zoonosis transmitted by the bite of infected Phlebotominae flies that act as vectors. CanL is a very serious disease that usually produces death when remains untreated and can be a focus of transmission to other dogs or humans. Infected dogs and other domestic and wild animals act as reservoirs and are a real threat to uninfected/healthy dogs and humans in endemic areas where the sand flies are present. Prevention of new infections in dogs can help to stop the current increase of the disease in humans, reinforcing the concept of “One Health” approach. The management of CanL is being performed using prophylactic measures in healthy dogs − insecticides impregnated in collars or immunostimulants applied by spot-on devices − and chemotherapy in animals that suffer from the disease. Antimonials as first-line monotherapy have proven efficacy in reducing most of the clinical signs of CanL, but they need to be administered during several days, and no complete parasite clearance is achieved, favouring the presence of relapses among treated dogs. Therefore, new drugs, such as miltefosine, or combinations of this drug or antimonials with allopurinol are in the pipeline of clinical treatment of CanL. Recently, there has been an emergence of protective − prophylactic − and curative − autogenous vaccines − immunotherapy tools to face CanL, whose results are still under study. This review highlights the current use of preventive and eradicative weapons to fight against this disease, which is a scourge for dogs and a continuous threat to human beings.

ACS Style

Rosa M Reguera; Miguel Morán; Yolanda Pérez-Pertejo; Carlos Garcia-Estrada; Rafael Balaña-Fouce. Current status on prevention and treatment of canine leishmaniasis. Veterinary Parasitology 2016, 227, 98 -114.

AMA Style

Rosa M Reguera, Miguel Morán, Yolanda Pérez-Pertejo, Carlos Garcia-Estrada, Rafael Balaña-Fouce. Current status on prevention and treatment of canine leishmaniasis. Veterinary Parasitology. 2016; 227 ():98-114.

Chicago/Turabian Style

Rosa M Reguera; Miguel Morán; Yolanda Pérez-Pertejo; Carlos Garcia-Estrada; Rafael Balaña-Fouce. 2016. "Current status on prevention and treatment of canine leishmaniasis." Veterinary Parasitology 227, no. : 98-114.

Journal article
Published: 01 August 2015 in Biochimie
Reads 0
Downloads 0

Penicillin biosynthesis in Penicillium chrysogenum (re-identified as Penicillium rubens) is a good example of a biological process subjected to complex global regulatory networks and serves as a model to study fungal secondary metabolism. The winged-helix family of transcription factors recently described, which includes the forkhead type of proteins, is a key type of regulatory proteins involved in this process. In yeasts and humans, forkhead transcription factors are involved in different processes (cell cycle regulation, cell death control, pre-mRNA processing and morphogenesis); one member of this family of proteins has been identified in the P. chrysogenum genome (Pc18g00430). In this work, we have characterized this novel transcription factor (named PcFKH1) by generating knock-down mutants and overexpression strains. Results clearly indicate that PcFKH1 positively controls antibiotic biosynthesis through the specific interaction with the promoter region of the penDE gene, thus regulating penDE mRNA levels. PcFKH1 also binds to the pcbC promoter, but with low affinity. In addition, it also controls other ancillary genes of the penicillin biosynthetic process, such as phlA (encoding phenylacetyl CoA ligase) and ppt (encoding phosphopantetheinyl transferase). PcFKH1 also plays a role in conidiation and spore pigmentation, but it does not seem to be involved in hyphal morphology or cell division in the improved laboratory reference strain Wisconsin 54-1255. A genome-wide analysis of processes putatively coregulated by PcFKH1 and PcRFX1 (another winged-helix transcription factor) in P. chrysogenum provided evidence of the global effect of these transcription factors in P. chrysogenum metabolism.

ACS Style

Rebeca Domínguez-Santos; Carlos García-Estrada; Katarina Kosalkova; Carlos Prieto; Irene Santamarta; Juan-Francisco Martín. PcFKH1, a novel regulatory factor from the forkhead family, controls the biosynthesis of penicillin in Penicillium chrysogenum. Biochimie 2015, 115, 162 -176.

AMA Style

Rebeca Domínguez-Santos, Carlos García-Estrada, Katarina Kosalkova, Carlos Prieto, Irene Santamarta, Juan-Francisco Martín. PcFKH1, a novel regulatory factor from the forkhead family, controls the biosynthesis of penicillin in Penicillium chrysogenum. Biochimie. 2015; 115 ():162-176.

Chicago/Turabian Style

Rebeca Domínguez-Santos; Carlos García-Estrada; Katarina Kosalkova; Carlos Prieto; Irene Santamarta; Juan-Francisco Martín. 2015. "PcFKH1, a novel regulatory factor from the forkhead family, controls the biosynthesis of penicillin in Penicillium chrysogenum." Biochimie 115, no. : 162-176.

Book chapter
Published: 16 June 2015 in Fungal Biology
Reads 0
Downloads 0

Secondary metabolites produced by fungi have a tremendous impact on the human society; some are exploited for their antibiotic and pharmaceutical activities, others are involved in disease interactions with other organisms such as plants or animals. The Springer book series on fungal biology includes two volumes on the Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites accommodating the importance of these substances and the tremendous progress in the research on fungal secondary metabolism during recent years. While the first volume was published in 2014, you now have the second volume in your hand. In this introductory chapter, we bridge volume I—which covers the best-studied fungal secondary metabolites such as the penicillin and cephalosporin antibiotics, the immunosuppressive cyclosporine A, the anticholesterolemic agents lovastatin and compactin, the mycotoxins aflatoxin, ochratoxin A, roquefortine C, gibberellins, fusarins, fusaric acid and ergot alkaloids, the carotenoid, xanthophyll, bikaverin and fusarubin pigments, the pyripyropene meroterpenoids, and the iron-chelating siderophores—with other important aspects of secondary metabolites comprised in the second volume. Articles in the latter cover the spectrum from key transcriptional players in the regulation of secondary metabolite biosynthesis and its epigenetic control to approaches for the detection of new gene clusters and substances by genome mining, metagenomics/metatranscriptomics and metabolomics to the use of secondary metabolite profiles in fungal chemotaxonomy. Further reviews deal with the still underexplored fungal endophytes as a reservoir of novel biologically active natural products, the role of secondary metabolites in the interaction of fungi with plants and animals, and with a special group of fungal peptide secondary metabolites, the peptaibols.

ACS Style

Rer. Nat Susanne Zeilinger; Carlos García-Estrada; Juan-Francisco Martín. Fungal Secondary Metabolites in the “OMICS” Era. Fungal Biology 2015, 1 -12.

AMA Style

Rer. Nat Susanne Zeilinger, Carlos García-Estrada, Juan-Francisco Martín. Fungal Secondary Metabolites in the “OMICS” Era. Fungal Biology. 2015; ():1-12.

Chicago/Turabian Style

Rer. Nat Susanne Zeilinger; Carlos García-Estrada; Juan-Francisco Martín. 2015. "Fungal Secondary Metabolites in the “OMICS” Era." Fungal Biology , no. : 1-12.

Comparative study
Published: 22 May 2015 in Applied Microbiology and Biotechnology
Reads 0
Downloads 0

The production of mycotoxins and other secondary metabolites in Penicillium roqueforti is of great interest because of its long history of use in blue-veined cheese manufacture. In this article, we report the cloning and characterization of the roquefortine gene cluster in three different P. roqueforti strains isolated from blue cheese in the USA (the type strain), France, and the UK (Cheshire cheese). All three strains showed an identical roquefortine gene cluster organization and almost identical (98-99%) gene nucleotide sequences in the entire 16.6-kb cluster region. When compared with the Penicillium chrysogenum roquefortine/meleagrin seven-gene cluster, the P. roqueforti roquefortine cluster contains only four genes (rds, rdh, rpt, and gmt) encoding the roquefortine dipeptide synthetase, roquefortine D dehydrogenase, roquefortine prenyltransferase, and a methyltransferase, respectively. Silencing of the rds or rpt genes by the RNAi strategy reduced roquefortine C production by 50% confirming the involvement of these two key genes in roquefortine biosynthesis. An additional putative gene, orthologous of the MFS transporter roqT, is rearranged in all three strains as a pseudogene. The same four genes and a complete (not rearranged) roqT, encoding a MFS transporter containing 12 TMS domains, occur in the seven-gene cluster in P. chrysogenum although organized differently. Interestingly, the two "late" genes of the P. chrysogenum roquefortine/meleagrin gene cluster that convert roquefortine C to glandicoline B and meleagrin are absent in the P. roqueforti four-gene cluster. No meleagrin production was detected in P. roqueforti cultures grown in YES medium, while P. chrysogenum produces meleagrin in these conditions. No orthologous genes of the two missing meleagrin synthesizing genes were found elsewhere in the recently released P. roqueforti genome. Our data suggest that during evolution, the seven-gene cluster present in P. chrysogenum, and probably also in other glandicoline/meleagrin producing fungi, has been trimmed down to a short cluster in P. roqueforti leading to the synthesis of roquefortine C rather than meleagrin as a final product.

ACS Style

Katarina Kosalkova; Rebeca Domínguez-Santos; Monika Coton; Emmanuel Coton; Carlos Garcia-Estrada; P. Liras; J. F. Martín. A natural short pathway synthesizes roquefortine C but not meleagrin in three different Penicillium roqueforti strains. Applied Microbiology and Biotechnology 2015, 99, 7601 -7612.

AMA Style

Katarina Kosalkova, Rebeca Domínguez-Santos, Monika Coton, Emmanuel Coton, Carlos Garcia-Estrada, P. Liras, J. F. Martín. A natural short pathway synthesizes roquefortine C but not meleagrin in three different Penicillium roqueforti strains. Applied Microbiology and Biotechnology. 2015; 99 (18):7601-7612.

Chicago/Turabian Style

Katarina Kosalkova; Rebeca Domínguez-Santos; Monika Coton; Emmanuel Coton; Carlos Garcia-Estrada; P. Liras; J. F. Martín. 2015. "A natural short pathway synthesizes roquefortine C but not meleagrin in three different Penicillium roqueforti strains." Applied Microbiology and Biotechnology 99, no. 18: 7601-7612.

Book chapter
Published: 01 January 2015 in Microencapsulation and Microspheres for Food Applications
Reads 0
Downloads 0
ACS Style

Iñigo Arozarena; Victor M. Balcão; Soraia Vilela Borges; Diego Alvarenga Botrel; Jennifer Burgain; Marco V. Chaud; Magda Corgneau; Ziortza Cruz; Gabriel Davidov-Pardo; Anna Chiara De Prisco; Patrick Degen; Francesco Donsì; Milla Gabriela Dos Santos; Daniel Edelhoff; Carmen Sílvia Favaro-Trindade; Regiane Victória De Barros Fernandes; Vincenzo Fogliano; Claire Gaiani; Gabriela Gallardo; Chunmei Gao; Carlos García-Estrada; Cássia A. Glasser; Vural Gökmen; Lía V. Guardiola; Stefan Henning; Laura G. Hermida; Mingzhu Liu; Weilin Liu; Zhen Liu; Shaoyu Lü; María R. Marin-Arroyo; Gian Luigi Mauriello; David Julian McClements; Montserrat Navarro; Idoia Olabarrieta; Graciela W. Padua; Jerome P. Paques; Sandra Rainieri; Glaucia Aguiar Rocha-Selmi; Leonard M.C. Sagis; Joël Scher; Mariarenata Sessa; Harjinder Singh; Dieter Suter; Ismail Tontul; Ayhan Topuz; Antonio Dario Troise; Marta M.D.C. Vila; Paola Vitaglione; Bi-Cheng Wu; Jinlong Yang; Aiqian Ye; Xinyu Zhang. List of Contributors. Microencapsulation and Microspheres for Food Applications 2015, 1 .

AMA Style

Iñigo Arozarena, Victor M. Balcão, Soraia Vilela Borges, Diego Alvarenga Botrel, Jennifer Burgain, Marco V. Chaud, Magda Corgneau, Ziortza Cruz, Gabriel Davidov-Pardo, Anna Chiara De Prisco, Patrick Degen, Francesco Donsì, Milla Gabriela Dos Santos, Daniel Edelhoff, Carmen Sílvia Favaro-Trindade, Regiane Victória De Barros Fernandes, Vincenzo Fogliano, Claire Gaiani, Gabriela Gallardo, Chunmei Gao, Carlos García-Estrada, Cássia A. Glasser, Vural Gökmen, Lía V. Guardiola, Stefan Henning, Laura G. Hermida, Mingzhu Liu, Weilin Liu, Zhen Liu, Shaoyu Lü, María R. Marin-Arroyo, Gian Luigi Mauriello, David Julian McClements, Montserrat Navarro, Idoia Olabarrieta, Graciela W. Padua, Jerome P. Paques, Sandra Rainieri, Glaucia Aguiar Rocha-Selmi, Leonard M.C. Sagis, Joël Scher, Mariarenata Sessa, Harjinder Singh, Dieter Suter, Ismail Tontul, Ayhan Topuz, Antonio Dario Troise, Marta M.D.C. Vila, Paola Vitaglione, Bi-Cheng Wu, Jinlong Yang, Aiqian Ye, Xinyu Zhang. List of Contributors. Microencapsulation and Microspheres for Food Applications. 2015; ():1.

Chicago/Turabian Style

Iñigo Arozarena; Victor M. Balcão; Soraia Vilela Borges; Diego Alvarenga Botrel; Jennifer Burgain; Marco V. Chaud; Magda Corgneau; Ziortza Cruz; Gabriel Davidov-Pardo; Anna Chiara De Prisco; Patrick Degen; Francesco Donsì; Milla Gabriela Dos Santos; Daniel Edelhoff; Carmen Sílvia Favaro-Trindade; Regiane Victória De Barros Fernandes; Vincenzo Fogliano; Claire Gaiani; Gabriela Gallardo; Chunmei Gao; Carlos García-Estrada; Cássia A. Glasser; Vural Gökmen; Lía V. Guardiola; Stefan Henning; Laura G. Hermida; Mingzhu Liu; Weilin Liu; Zhen Liu; Shaoyu Lü; María R. Marin-Arroyo; Gian Luigi Mauriello; David Julian McClements; Montserrat Navarro; Idoia Olabarrieta; Graciela W. Padua; Jerome P. Paques; Sandra Rainieri; Glaucia Aguiar Rocha-Selmi; Leonard M.C. Sagis; Joël Scher; Mariarenata Sessa; Harjinder Singh; Dieter Suter; Ismail Tontul; Ayhan Topuz; Antonio Dario Troise; Marta M.D.C. Vila; Paola Vitaglione; Bi-Cheng Wu; Jinlong Yang; Aiqian Ye; Xinyu Zhang. 2015. "List of Contributors." Microencapsulation and Microspheres for Food Applications , no. : 1.

Book chapter
Published: 01 January 2015 in Microencapsulation and Microspheres for Food Applications
Reads 0
Downloads 0
ACS Style

Ziortza Cruz; Carlos Garcia-Estrada; Idoia Olabarrieta; Sandra Rainieri. Lipid Nanoparticles. Microencapsulation and Microspheres for Food Applications 2015, 313 -331.

AMA Style

Ziortza Cruz, Carlos Garcia-Estrada, Idoia Olabarrieta, Sandra Rainieri. Lipid Nanoparticles. Microencapsulation and Microspheres for Food Applications. 2015; ():313-331.

Chicago/Turabian Style

Ziortza Cruz; Carlos Garcia-Estrada; Idoia Olabarrieta; Sandra Rainieri. 2015. "Lipid Nanoparticles." Microencapsulation and Microspheres for Food Applications , no. : 313-331.