Diana Bedolla

Dr. Diana Bedolla

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Research Focus & Metrics

Academic Career Length

18Years (2009-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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28 June 2021 in Nature Communications
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Journal Article
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HBHenricus T. S. Boschker
Henricus T. S. Boschker
PCPerran L. M. Cook
Perran L. M. Cook
LPLubos Polerecky
Lubos Polerecky
RERaghavendran Thiruvallur Eachambadi
Raghavendran Thiruvallur Eachambadi
HLHelena Lozano
Helena Lozano
FMFilip Meysman
Filip Meysman
Filamentous cable bacteria display long-range electron transport, generating electrical currents over centimeter distances through a highly ordered network of fibers embedded in their cell envelope. The conductivity of these periplasmic wires is exceptionally high for a biological material, but their chemical structure and underlying electron transport mechanism remain unresolved. Here, we combine high-resolution microscopy, spectroscopy, and chemical imaging on individual cable bacterium filaments to demonstrate that the periplasmic wires consist of a conductive protein core surrounded by an insulating protein shell layer. The core proteins contain a sulfur-ligated nickel cofactor, and conductivity decreases when nickel is oxidized or selectively removed. The involvement of nickel as the active metal in biological conduction is remarkable, and suggests a hitherto unknown form of electron transport that enables efficient conduction in centimeter-long protein structures.

30 November 2016 in Analytical Chemistry
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Journal Article
Closed access
PZPaolo Zucchiatti
Paolo Zucchiatti
EMElisa Mitri
Elisa Mitri
SKSaša Kenig
Saša Kenig
FBFulvio Billè
Fulvio Billè
GKGeorge Kourousias
George Kourousias
Lisa VaccariLisa Vaccari
Lisa Vaccari

27 May 2021 in Angewandte Chemie International Edition
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Bayden Robert WoodBayden Robert Wood
Bayden Robert Wood
KKKamila Kochan
Kamila Kochan
DBDiana Bedolla
Diana Bedolla
NSNatalia Salazar‐Quiroz
Natalia Salazar‐Quiroz
SGSamantha Leigh Grimley
Samantha Leigh Grimley
PHPhilip Heraud
Philip Heraud

14 May 2015 in Scientific Reports
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Journal Article
Open access
AGAlessandra Gianoncelli
Alessandra Gianoncelli
Lisa VaccariLisa Vaccari
Lisa Vaccari
GKG. Kourousias
G. Kourousias
DCD. Cassese
D. Cassese
DBDiana Bedolla
Diana Bedolla
MKM. Kiskinova
M. Kiskinova
Radiation damage of biological samples remains a limiting factor in high resolution X-ray microscopy (XRM). Several studies have attempted to evaluate the extent and the effects of radiation damage, proposing strategies to minimise or prevent it. The present work aims to assess the impact of soft X-rays on formalin fixed cells on a systematic manner. The novelty of this approach resides on investigating the radiation damage not only with XRM, as often reported in relevant literature on the topic, but by coupling it with two additional independent non-destructive microscopy methods: Atomic Force Microscopy (AFM) and FTIR Microscopy (FTIRM). Human Embryonic Kidney 293 cells were exposed to different radiation doses at 1 keV. In order to reveal possible morphological and biochemical changes, the irradiated cells were systematically analysed with AFM and FTIRM before and after. Results reveal that while cell morphology is not substantially affected, cellular biochemical profile changes significantly and progressively when increasing dose, resulting in a severe breakdown of the covalent bonding network. This information impacts most soft XRM studies on fixed cells and adds an in-depth understanding of the radiation damage for developing better prevention strategies.

1 May 2019 in Journal of Hazardous Materials
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Journal Article
Closed access
MBMansi Bakshi
Mansi Bakshi
CLClarisse Liné
Clarisse Liné
DBDiana Bedolla
Diana Bedolla
RSRicardo José Stein
Ricardo José Stein
RKRalf Kaegi
Ralf Kaegi
CLCamille Larue
Camille Larue

30 May 2018 in The Analyst
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Journal Article
Closed access
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Elisabetta GiorginiElisabetta Giorgini
Elisabetta Giorgini
SSSimona Sabbatini
Simona Sabbatini
RRRomina Rocchetti
Romina Rocchetti
Valentina NotarstefanoValentina Notarstefano
Valentina Notarstefano
CRCorrado Rubini
Corrado Rubini
Lisa VaccariLisa Vaccari
Lisa Vaccari
In the present study, human primary oral squamous carcinoma cells treated with cisplatin and 5-fluorouracil were analyzed, for the first time, by in vitro FTIR Microspectroscopy (FTIRM), to improve the knowledge on the biochemical pathways activated by these two chemotherapy drugs. To date, most of the studies regarding FTIRM cellular analysis have been executed on fixed cells from immortalized cell lines. FTIRM analysis performed on primary tumor cells under controlled hydrated conditions provides more reliable information on the biochemical processes occurring in in vivo tumor cells. This spectroscopic analysis allows to get on the same sample and at the same time an overview of the composition and structure of the most remarkable cellular components. In vitro FTIRM analysis of primary oral squamous carcinoma cells evidenced a time-dependent drug-specific cellular response, also including apoptosis triggering. Furthermore, the univariate and multivariate analyses of IR data evidenced meaningful spectroscopic differences ascribable to alterations affecting cellular proteins, lipids and nucleic acids. These findings suggest for the two drugs different pathways and extents of cellular damage, not provided by conventional cell-based assays (MTT assay and image-based cytometry).