Purpose: Although chemotherapy is one of the standard treatments for gastric cancer, the disease’s resistance mechanisms continue to limit the survival rates. B7H6 (NCR3LG1), an immune checkpoint belonging to the B7 family, is significantly overexpressed in gastric cancer. This work investigated the possibility of using B7H6 suppression to improve the effectiveness of the widely used chemotherapy medication docetaxel. Materials and Methods: In this study, MKN-45 gastric cancer cells were transfected for 24 h with siRNA targeting B7H6, and then, docetaxel was added at optimal inhibitory doses (IC25 and IC50). To assess the impact of this combination therapy, cellular viability, proliferation, and migration were assessed using MTT assay, colony-forming unit assay, and wound-healing assay, respectively. Additionally, apoptosis and cell cycle status were evaluated by flow cytometry. Moreover, using qRT-PCR, the gene expression of B7H6 and indicators associated with apoptosis was also examined. Results: The sensitivity of MKN-45 cells to docetaxel was greatly increased by the siRNA-mediated knockdown of B7H6, resulting in a decrease in the drug’s IC50 value. When compared to each therapy alone, the combination of B7H6 siRNA plus docetaxel at IC50 levels exhibited a significant increase in apoptosis rate. The volume of cells arrested at the sub-G1 and G2-M phase was shown to rise when B7H6 siRNA transfection was combined with docetaxel. Furthermore, the combination treatment significantly decreased the ability of cells to migrate and form colonies. Conclusions: B7H6 suppression increases the susceptibility of MKN-45 gastric cancer cells to docetaxel treatment, resulting in decreased cellular proliferation and increased rates of apoptosis. The present work underscores the possibility of enhancing treatment results in gastric cancer by merging conventional chemotherapy with gene-silencing approaches.

Background/aim: Thermopsis turcica is a perennial species endemic to Turkey and different extracts of T. turcica have an antiproliferative effect on cancer cells, but there has not been any report on HeLa (human cervical cancer) cells. Materials and methods: To get a better understanding of the molecular mechanism of anticancer activity of methanolic extracts of leaves (LE) and flowers (FE) of T. turcica, we employed 2-DE-based proteomics to explore the proteins involved in anticancer activity in HeLa cells. Results: T. turcica extracts showed a potent cytotoxic effect on HeLa cells with the IC50 values of 1.75 mg/mL for LE and 3.25 mg/mL for FE. The induction of apoptosis by LE and FE was also consistent with increased expression of caspase mRNAs and DNA fragmentation. In terms of the proteomic approach, 27 differentially expressed proteins were detected and identified through MALDI-TOF/TOF mass spectrometry. These altered proteins were involved in cytoskeleton organization and movement, protein folding, proteolysis and translation, cell cycle and proliferation, signal transduction, cell redox homeostasis, and metabolism. Conclusion: Up-regulation of protein disulfide isomerases and down-regulation of Rho GDP-dissociation inhibitor, heterogeneous nuclear ribonucleoproteins, and heat shock proteins may contribute to the induction of apoptosis and arresting of the cell cycle in HeLa cells.

AMAÇ: İnfertilite tanısı almış ve karyotip analizi yapılmış 391 vaka (178 kadın, 213 erkek) grubu ve 40 çiftten oluşan kontrol grubunun kromozomal polimorfizmler açısından karşılaştırılması amaçlandı. GEREÇ VE YÖNTEM: Vaka grubunun kromozom polimorfizmleri, arşivdeki dosyalar ve preparatlar kullanılarak retrospektif değerlendirildi. Kontrol grubuna ait kan örneklerinden kapalı lenfosit kültürü yapılarak karyotip analizi yapıldı. BULGULAR: Toplam satellit polimorfizmleri (13 ps+,14 ps+, 15 ps+, 21 ps+ ve 22 ps+) kadın vaka grubunda kontrole göre önemli düzeyde yüksek bulundu. Vaka grubunda 1qh+, 9qh-, inv(9), 13ps+, 14ps+, 15ps+, 16qh+, 21ps+ ve 22ps+ polimorfizmlerinin dağılımlarında cinsiyetle ilişkili bir farklılık belirlenmedi. İnfertil kadınlarda en sık 9qh+ (%14,04) varyantına rastlandı. Polimorfizm 9qh+ oranı, kontrol grubuna göre vaka grubunda yüksek olmasına rağmen fark anlamsız bulundu. SONUÇ: İnfertilite ve kromozom polimorfizmleri arasında olası bir ilişkinin varlığına dair bulgular elde edilmiş olmasına rağmen, bu ilişkinin daha net ortaya konabilmesi için örneklem sayısının artırılması ve geniş kapasiteli yeni teknolojilerin kullanıldığı çalışmaların yapılması gereklidir.

Aim: Castor oil has recently gained attention for its wound-healing properties due to its rich phytochemical composition. In particular, the ricinoleic acid exhibits anti-inflammatory, antibacterial, and skin barrier-supporting effects. In addition, the development of environmentally sustainable and biodegradable materials has increased the interest in plant-based formulations. This study aimed to evaluate the wound healing potential of castor oil-based biocompatible greases at different concentrations using an in vitro scratch assay on human keratinocyte (HaCaT) cells. Methods: HaCaT cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin–Streptomycin under standard incubation conditions (5% CO₂, 37 °C). Once confluent, a scratch was created in the cell monolayer using a sterile 200 μL pipette tip. Castor oil-based biocompatible greases at different concentrations (0.1%, 1%, 5%, and 10%) were applied to the experimental groups, while standard culture medium served as the negative control. The term “castor oil-based biocompatible grease” refers to castor oil applied at different concentrations in culture medium, representing a component of a proposed grease formulation. Cells were incubated for 0, 12, 24, and 48 hours. Wound closure was evaluated by inverted microscopy and analyzed quantitatively using ImageJ software. Results: Quantitative analyses demonstrated a concentration-dependent effect of castor oil-based grease on wound closure. The 5% concentration group showed the greatest wound closure rate at 12 hours, comparable to the negative control. Lower concentrations (0.1% and 1%) exhibited slower closure rates, whereas the 10% group showed reduced cell migration and morphological deterioration, suggesting possible cytotoxicity. The order of wound closure effectiveness was 5% castor oil > negative control > Tween control > 10% > 0.1% > 1%. Conclusion: Castor oil-based biocompatible greases influenced wound healing in a dose-dependent manner, with optimal effects observed at 5% concentration. These findings highlight the potential of castor oil-based greases as biocompatible, biodegradable, and environmentally sustainable biomaterials for wound healing applications, warranting further preclinical and clinical investigations.