: To determine the fluconazole and Amphotericin B susceptibility pattern among isolates by disk diffusion, Vitek-2 method, and micro-broth dilution methods (MBD).Fungal infections are now becoming more prevalent than bacterial in causing opportunistic and nosocomial infections. are among the most common invasive fungi that are seen in most patients with predisposing co-morbidities such as diabetes and hypertension. Therefore; identification of the species and antifungal susceptibility testing is essential for proper patient management as various species respond differently to antifungals and for the prevention of emergence of drug resistance. isolated from different specimens were included in the study, speciation of the isolates was achieved by Vitek 2 automated machine and also with corn meal agar inoculation (CMA). The comparison of antifungal susceptibility testing was done by three different methods which included Vitek 2 systems, disk diffusion, and Micro-broth dilution methods. The antifungal susceptibility was tested for fluconazole and Amphotericin B. A total of 50 isolates were randomly selected and speciated. Species-wise distribution showed s to be the most common one accounting for 34 (69%) followed by 12(24%) and 4 (12%) of them were . The overall resistance among these isolates was as follows: by micro –broth dilution fluconazole resistance to was 6% and to Amphotericin B was 8%. By disc diffusion, fluconazole resistance was 10%, Amphotericin B was 14%. With the Vitek 2 system, fluconazole and Amphotericin B showed resistance of 10% and 6% respectively. Speciation and Antifungal susceptibility testing of solates are of great significance regardless of the specimen isolated from, which helps in the management and knowing the epidemiology of the susceptibility pattern of the . For this to become a reality a reliable, easy, and sensitive method of antifungal susceptibility needs to be used in every Microbiology laboratory as a routine practice.

Dengue fever is one of the common viral illnesses linked with significant morbidity and mortality. Of late, there is rapid increase in dengue cases in India. The present study aimed to known dengue seropositivity in different seasons of a year, other laboratory parameters and its geographical distribution. During the study period of 15 months 4172 patients hospitalized with probable dengue fever were included. Dengue serology was done for all cases by ELISA(J. Mitra & Co). Demographic details were taken to create geographical maps of the disease. Of the 4172 suspected dengue cases, 817 had confirmed dengue illness. Of these 817 cases, 584 showed Ns1 antigen (71.4%), 150 IgM antibody (18.3%), and 38 had both Ns1 and Ig M (4.6%). Platelet count less than 1,00,000 were seen in 566 (69.27%) patients. Dengue was noticeably more during monsoon time (June to September) of a year. Through geographical maps major clusters were noticed urban areas of Mysuru and Krishnaraja Nagara , while few clusters were also observed in Periyapatna and T Narsipura taluks of Mysuru district. To minimize the load of dengue cases and its death rate better community awareness and vector control measures need to be strengthened during monsoon especially in areas where burden of the disease is more. This study helps in early preparedness of the authorities concerned in controlling possible epidemics in future.

Deciphering fosfomycin\\\'s antimicrobial action: comparative evaluation of susceptibility testing techniques on clinical isolates - IJMR- Print ISSN No: - 2394-546X Online ISSN No:- 2394-5478 Article DOI No:- 10.18231/j.ijmr.2025.013, Indian Journal of Microbiology Research-Indian J Microbiol Res

Brucellosis distributed worldwide is a classical zoonotic disease. Complications associated with gangrene are quite uncommon and may or might not occur during the course of the disease. Here, we report a pediatric age group patient who presented with fever and gangrene over the upper and lower limbs and abdomen associated with brucellosis.

Introduction: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2), which caused primary COVID-19 infection in millions worldwide, raised significant concerns as viral mutations emerged, leading to reinfections among previously infected and vaccinated individuals. This phenomenon became particularly evident during the second and third waves of the COVID-19 pandemic in India. Methods: The current study observed the rates of reinfections due to SARS-CoV-2 and their relation to vaccination details by retrospectively analyzing the data of patients who had reinfections during the second and third waves of COVID-19. Results: Among the 458 study participants, 62/458 (13.54%) with primary infection had confirmed re-infection. Of the 458 people infected with COVID-19 for the first time, 60 (13%) were asymptomatic and 398 (87%) were symptomatic. The most frequently observed symptoms were fever during primary (70.3%) and re-infection (72.58%). A statistically significant association was observed between vomiting symptoms and infection status ( p < 0.01). Conclusions: SARS-CoV-2 is re-emerging with new variants and varied presentations, posing a challenge to clinicians regarding specific diagnoses. The symptoms of COVID-19 during primary and re-infection did not differ greatly. Although vaccination protects individuals against reinfections, the exact duration of protection remains unknown and may necessitate booster doses at frequent intervals.