As a group of emerging contaminants of global concern, tire additives and their transformation products (TATPs) are causing a severe threat to aquatic ecosystems, particularly the highly lethal effects of N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine quinone (6PPD-Q) on certain fish species. Yet, the contamination status of TATPs in the lake ecosystems remains largely uncharacterized. This study conducted the first nationwide monitoring of the distribution characteristics of TATPs in 208 lake sediments collected from five lake regions across China. All the 13 TATPs were identified in lake sediments, with the total levels varying between 1.4 and 1355 ng/g, and 4-hydroxydiphenylamine (4-OH-PPD) as the most dominant. The total levels of TATPs decreased in the following order: Yunnan-Guizhou Plateau > Inner Mongolia-Xinjiang Region, Eastern Plain > Qinghai-Tibet Plateau, and Northeast Plain (p < 0.05). The geographical distribution of TATPs in lake sediments was significantly driven by total organic carbon content, temperature, and population density. N,N'-di-2-naphthyl-p-phenylenediamine, 6PPD-Q, N,N'-diphenyl-p-phenylenediamine, and 4-OH-PPD belonged to high-priority contaminants. Our study emphasizes that emerging pollutant TATPs place significant pressure on lake ecosystems and deserve urgent attention.