The relationship between detritivore diversity and decomposition can provide information on how biogeochemical cycles are affected by ongoing rates of extinction, but such evidence has come mostly from local studies and microcosm experiments. We conducted a globally distributed experiment (38 streams across 23 countries in 6 continents) using standardised methods to test the hypothesis that detritivore diversity enhances litter decomposition in streams, to establish the role of other characteristics of detritivore assemblages (abundance, biomass and body size), and to determine how patterns vary across realms, biomes and climates. We observed a positive relationship between diversity and decomposition, strongest in tropical areas, and a key role of abundance and biomass at higher latitudes. Our results suggest that litter decomposition might be altered by detritivore extinctions, particularly in tropical areas, where detritivore diversity is already relatively low and some environmental stressors particularly prevalent.

Plant litter functional diversity effects on instream decomposition change across latitudes.

This article reviews the impact of single-cell sequencing (SCS) on cancer biology research. SCS has revolutionized our understanding of cancer and tumor heterogeneity, clonal evolution, and the complex interplay between cancer cells and tumor microenvironment. SCS provides high-resolution profiling of individual cells in genomic, transcriptomic, and epigenomic landscapes, facilitating the detection of rare mutations, the characterization of cellular diversity, and the integration of molecular data with phenotypic traits. The integration of SCS with multi-omics has provided a multidimensional view of cellular states and regulatory mechanisms in cancer, uncovering novel regulatory mechanisms and therapeutic targets. Advances in computational tools, artificial intelligence (AI), and machine learning have been crucial in interpreting the vast amounts of data generated, leading to the identification of new biomarkers and the development of predictive models for patient stratification. Furthermore, there have been emerging technologies such as spatial transcriptomics and in situ sequencing, which promise to further enhance our understanding of tumor microenvironment organization and cellular interactions. As SCS and its related technologies continue to advance, they are expected to drive significant advances in personalized cancer diagnostics, prognosis, and therapy, ultimately improving patient outcomes in the era of precision oncology.

Freshwater organisms are often sensitive to pesticides, but their sensitivity varies across different taxa and with pesticide type and action mode, as shown by multiple acute toxicity tests. Such variability hampers predictions about how freshwater ecosystems may be altered by pesticide toxicity, which is especially critical for understudied areas of the world such as the tropics. Furthermore, there is little information about the sensitivity of some organisms that are key components of stream food webs; this is the case of litter-feeding detritivorous invertebrates, which contribute to the fundamental process of litter decomposition. Here, we examined the sensitivity of three common detritivores [Anchytarsus sp. (Coleoptera: Ptilodactylidae), Hyalella sp. (Amphipoda: Hyalellidae) and Lepidostoma sp. (Trichoptera: Lepidostomatidae)] to three pesticides commonly used (the insecticides bifenthrin and chlorpyrifos and the fungicide chlorothalonil) using acute (48 or 96 h) toxicity tests. Our study demonstrates that common-use pesticides provoke the mortality of half their populations at concentrations of 0.04-2.7 μg L-1. We found that all species were sensitive to the three pesticides, with the highest sensitivity found for chlorpyrifos. Additionally, we used the approach of species sensitivity distributions (SSD) to compare our study species with Daphnia magna and other temperate and tropical invertebrates. We found that the study species were among the most sensitive species to chlorpyrifos and chlorothalonil. Our results suggest that tropical detritivores merit special attention in ecological risk assessment of pesticides and highlight the need for accurate ecotoxicological information from ecologically relevant species in the tropics.

Stream ecosystem functioning is often impaired by warming and salinization, but the joint effect of both environmental stressors on key processes such as leaf litter decomposition is virtually unknown, particularly in the tropics. We experimentally explored how increased water temperature (26, 29 and 32°C) and salinity (no salt addition, 0.1, 1.0 and 10 g l⁻¹ NaCl added) affected the rates of total, microbial and detritivore-mediated decomposition, in stream microcosms containing leaf litter of Ficus insipida and larvae of Chironomus sp. collected from tropical streams. Effects of temperature were strong and consistent with previous findings: it promoted microbial decomposition and reduced decomposition mediated by detritivores, which showed greater feeding activity at 26°C. Salinity was negatively correlated with microbial decomposition at 32°C; it also had a negative influence on detritivore-mediated decomposition, which was nevertheless non-significant due to the high detritivore mortality at higher salinities. Notably, total decomposition was reduced with the joint presence of both factors (32°C and salt addition treatments, compared to 26°C and no salt addition), indicating the existence of additive effects and highlighting the relevance of multiple-stressor contexts when assessing the consequences of global change on stream ecosystems.

Cyanobacterial blooms are intensifying globally under climate warming, eutrophication, and hydrological alteration, yet most mechanistic understanding derives from temperate lakes. Tropical and neotropical freshwaters operate under persistently warm conditions, weak seasonality, and hydrological variability that can sustain extended bloom windows and alter toxin production patterns spatiotemporally, requiring targeted synthesis. This review synthesizes recent experimental and field evidence, complemented by foundational frameworks, to evaluate cyanobacterial diversity, functional ecology, and cyanotoxin dynamics in tropical freshwater habitats. We highlight recurring trait syndromes, coordinated sets of physiological and functional traits, that recur across warm systems, including buoyancy regulation, diazotrophy, and thermal tolerance, which confer competitive advantages under warm, nutrient-rich conditions. These traits are prominent in dominant genera such as Microcystis, Raphidiopsis, and Planktothrix. We assess how temperature, nutrient stoichiometry, water residence time, and light interact to modulate bloom persistence and toxin production. We summarize appropriate monitoring and management approaches suited to warm, hydrologically dynamic basins. These including strategies addressing internal loading and integrated early-warning frameworks combining molecular tools and remote sensing. Substantial gaps persist in toxin quantification, biogeochemical fluxes, molecular surveillance, and coordinated risk assessment across the tropics. We argue that region-specific, integrative frameworks are urgently needed to improve early-warning capacity and mitigate cyanoHAB risks in tropical freshwater ecosystems.