This page has only limited features, please log in for full access.

Unclaimed
Oleg V. Gorshkov
Kazan Institute of Biochemistry and Biophysics FRC Kazan Scientific Center of RAS Lobachevsky Str 2/31 Kazan 420111 Russia

Basic Info

Basic Info is private.

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Letter
Published: 23 April 2021 in New Phytologist
Reads 0
Downloads 0

Plant fibers constitute a major part of renewable biomass and are a valuable resource for various industries, composite material chemistry, and bioconversion processes. Plant fibers are specialized long cells with thick cell walls that are a convenient model for investigating fundamental issues such as individual cell biogenesis, cell wall formation, and cell specialization. Plant fibers usually accumulate secondary cell walls, which contain lignin, cellulose, and hemicelluloses like xylan, and are laid over thin primary cell walls.

ACS Style

Natalia Mokshina; Oleg Gorshkov; Hironori Takasaki; Hitomi Onodera; Shingo Sakamoto; Tatyana Gorshkova; Nobutaka Mitsuda. FIBexDB: a new online transcriptome platform to analyze development of plant cellulosic fibers. New Phytologist 2021, 231, 512 -515.

AMA Style

Natalia Mokshina, Oleg Gorshkov, Hironori Takasaki, Hitomi Onodera, Shingo Sakamoto, Tatyana Gorshkova, Nobutaka Mitsuda. FIBexDB: a new online transcriptome platform to analyze development of plant cellulosic fibers. New Phytologist. 2021; 231 (2):512-515.

Chicago/Turabian Style

Natalia Mokshina; Oleg Gorshkov; Hironori Takasaki; Hitomi Onodera; Shingo Sakamoto; Tatyana Gorshkova; Nobutaka Mitsuda. 2021. "FIBexDB: a new online transcriptome platform to analyze development of plant cellulosic fibers." New Phytologist 231, no. 2: 512-515.

Journal article
Published: 20 May 2020 in Industrial Crops and Products
Reads 0
Downloads 0

Plant fibers, owing to their unique properties, have been used by mankind for the production of textiles and composite materials for thousands of years. Currently, the use of plant fibers in various innovative fields has become very popular as they are an environmentally friendly, biodegradable, and renewable resource. Each of the numerous fiber-consuming industrial fields has special demands on the properties of fiber. The quality of plant fibers is a multicomponent parameter that depends on morphological and anatomical characteristics, as well as on the structure and proportions of fiber bundle chemical constituents. These features are formed during plant ontogenesis and can be subjected to management by manipulating the key stages of fiber development: fiber elongation by intrusive growth and cell wall thickening. To determine the molecular keys for manipulating certain fiber features related to crop quality, we summarized the RNA-Seq data obtained for different flax tissues and revealed the genes specifically upregulated in bast fibers. These genes were additionally subdivided into three groups considering the differential expression of the genes at the stage of fiber intrusive elongation versus the stage of cell wall thickening. The first group included genes specifically expressed in fibers without differential expression at the two stages. The second included those upregulated at the intrusive elongation stage compared to the further stage of development, and the third included those upregulated at tertiary cell wall deposition. The qPCR analysis of the expression level of the selected genes in fibers isolated from the developing plants of several varieties of fiber and linseed, Linum usitatissimum, as well as of two wild flax species revealed that the samples cluster into different groups in accordance with bast fiber yield and quality. The genes with tissue- and stage-specific characters of expression provide new insights into the mechanisms of fiber development and may serve as a target for molecular-genetic modulation of crop quality, as there is a high possibility of exclusively affecting bast fiber parameters, without influencing other tissues.

ACS Style

Natalia Mokshina; Oleg Gorshkov; Dmitry Galinousky; Tatyana Gorshkova. Genes with bast fiber-specific expression in flax plants - Molecular keys for targeted fiber crop improvement. Industrial Crops and Products 2020, 152, 112549 .

AMA Style

Natalia Mokshina, Oleg Gorshkov, Dmitry Galinousky, Tatyana Gorshkova. Genes with bast fiber-specific expression in flax plants - Molecular keys for targeted fiber crop improvement. Industrial Crops and Products. 2020; 152 ():112549.

Chicago/Turabian Style

Natalia Mokshina; Oleg Gorshkov; Dmitry Galinousky; Tatyana Gorshkova. 2020. "Genes with bast fiber-specific expression in flax plants - Molecular keys for targeted fiber crop improvement." Industrial Crops and Products 152, no. : 112549.

Journal article
Published: 19 February 2019 in Plants
Reads 0
Downloads 0

Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from 23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, and miR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phloem fiber development, was characterized from a miRNA-target point of view, giving new insights into its regulation.

ACS Style

Oleg Gorshkov; Tatyana Chernova; Natalia Mokshina; Natalia Gogoleva; Dmitry Suslov; Alexander Tkachenko; Tatyana Gorshkova. Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles. Plants 2019, 8, 47 .

AMA Style

Oleg Gorshkov, Tatyana Chernova, Natalia Mokshina, Natalia Gogoleva, Dmitry Suslov, Alexander Tkachenko, Tatyana Gorshkova. Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles. Plants. 2019; 8 (2):47.

Chicago/Turabian Style

Oleg Gorshkov; Tatyana Chernova; Natalia Mokshina; Natalia Gogoleva; Dmitry Suslov; Alexander Tkachenko; Tatyana Gorshkova. 2019. "Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles." Plants 8, no. 2: 47.

Chapter
Published: 10 June 2018 in Plant Biomechanics
Reads 0
Downloads 0

Plant fibers are the important elements to shape the mechanical properties of plant body, especially in the organs that have already ceased elongation. The major distinguishing parameters of fibers are a highly prosenchimatous cell shape and an increased cell wall thickness as compared to other types of plant cells. The increase of fiber cell length is largely achieved by intrusive growth—elongation with the increased rate as compared to the adjacent cells and squeezing between them along the middle lamellae. The highly pronounced intrusive growth is the cause of fiber bundle formation. Thickening of cell wall in fibers of many plant species is supplied by deposition of the tertiary cell wall (G-layer) of peculiar design and properties. Tension of cellulose microfibrils is developed in this cell wall layer, providing the contractile properties that permit to move plant organs. We summarize the currently available data describing the inherent to fibers mechanisms by which they attain their exclusive length (intrusive growth) and extreme cell wall thickness (tertiary cell wall deposition) and consider the results obtained by finite element modeling to realize the cause of cellulose microfibril tension. The suggested hypothesis is based on the entrapment of tissue- and stage-specific version of rhamnogalacturonan I between laterally interacting cellulose microfibrils.

ACS Style

Tatyana Gorshkova; Polina Mikshina; Anna Petrova; Tatyana Chernova; Natalia Mokshina; Oleg Gorshkov. Plants at Bodybuilding: Development of Plant “Muscles”. Plant Biomechanics 2018, 141 -163.

AMA Style

Tatyana Gorshkova, Polina Mikshina, Anna Petrova, Tatyana Chernova, Natalia Mokshina, Oleg Gorshkov. Plants at Bodybuilding: Development of Plant “Muscles”. Plant Biomechanics. 2018; ():141-163.

Chicago/Turabian Style

Tatyana Gorshkova; Polina Mikshina; Anna Petrova; Tatyana Chernova; Natalia Mokshina; Oleg Gorshkov. 2018. "Plants at Bodybuilding: Development of Plant “Muscles”." Plant Biomechanics , no. : 141-163.

Short communication
Published: 03 June 2018 in Plant Signaling & Behavior
Reads 0
Downloads 0

Flax phloem fibers act as constitutively formed “muscles” that support the vertical position of the high but narrow stem. The specific mechanical properties of flax fibers and of similar fibers in other plant species are provided by the development of tertiary cell wall with tensed cellulose microfibrils. The work of phloem fibers becomes especially pronounced during the restoration of stem vertical position if it was disturbed. Gravistimulation of flax plants induces considerable modification of phloem fibers at the pulling stem side – the lumen diameter increases, while the cell wall thickness goes down. Here we show that the action of phloem fibers as motors of stem vertical position restoration is coupled to the cell wall remodelling as well as the increase of osmolytes (mainly potassium and malate) content, and accumulation of the γ-amino-butyric acid that may be involved in signalling events. The molecular players that take part in these processes are suggested.

ACS Style

N. Mokshina; Oleg Gorshkov; N. Ibragimova; G. Pozhvanov; T. Gorshkova. Screenplay of flax phloem fiber behavior during gravitropic reaction. Plant Signaling & Behavior 2018, 13, e1486144 .

AMA Style

N. Mokshina, Oleg Gorshkov, N. Ibragimova, G. Pozhvanov, T. Gorshkova. Screenplay of flax phloem fiber behavior during gravitropic reaction. Plant Signaling & Behavior. 2018; 13 (6):e1486144.

Chicago/Turabian Style

N. Mokshina; Oleg Gorshkov; N. Ibragimova; G. Pozhvanov; T. Gorshkova. 2018. "Screenplay of flax phloem fiber behavior during gravitropic reaction." Plant Signaling & Behavior 13, no. 6: e1486144.

Review
Published: 02 April 2018 in Fibers
Reads 0
Downloads 0

Plant fibers find wide application in various fields that demand specific parameters of fiber quality. To develop approaches for the improvement of yield and quality of bast fibers, the knowledge of the fiber developmental stages and of the key molecular players that are responsible for a certain parameter, are vitally important. In the present review the key stages of fiber development, such as initiation, intrusive growth, and formation of thickened cell wall layers (secondary and tertiary cell walls) are considered, as well as the impact of each of these stages on the final parameters of fiber yield and quality. The problems and perspectives of crop quality regulation are discussed.

ACS Style

Natalia Mokshina; Tatyana Chernova; Dmitry Galinousky; Oleg Gorshkov; Tatyana Gorshkova. Key Stages of Fiber Development as Determinants of Bast Fiber Yield and Quality. Fibers 2018, 6, 20 .

AMA Style

Natalia Mokshina, Tatyana Chernova, Dmitry Galinousky, Oleg Gorshkov, Tatyana Gorshkova. Key Stages of Fiber Development as Determinants of Bast Fiber Yield and Quality. Fibers. 2018; 6 (2):20.

Chicago/Turabian Style

Natalia Mokshina; Tatyana Chernova; Dmitry Galinousky; Oleg Gorshkov; Tatyana Gorshkova. 2018. "Key Stages of Fiber Development as Determinants of Bast Fiber Yield and Quality." Fibers 6, no. 2: 20.

Research article
Published: 01 January 2018 in Functional Plant Biology
Reads 0
Downloads 0

Restoration of stem vertical position after plant inclination is a widely spread version of plant orientation in accordance with gravity vector direction. Gravitropic behaviour of flax plants involves the formation of curvature in stem region that has ceased elongation long in advance of stem inclination. The important participants of such behaviour are phloem fibres with constitutively formed tertiary cell wall (G-layer). We performed the large-scale transcriptome profiling of phloem fibres isolated from pulling and opposite sides of gravitropic curvature and compared with control plant fibres. Significant changes in transcript abundance take place for genes encoding proteins of several ion channels, transcription factors and other regulating elements. The largest number of upregulated genes belonged to the cell wall category; many of those were specifically upregulated in fibres of pulling stem side. The obtained data permit to suggest the mechanism of fibre participation in gravitropic reaction that involves the increase of turgor pressure and the rearrangements of cell wall structure in order to improve contractile properties, and to identify the regulatory elements that operate specifically in the fibres of the pulling stem side making gelatinous phloem fibres an important element of gravitropic response in herbaceous plants.

ACS Style

Oleg Gorshkov; Natalia Mokshina; Nadezda Ibragimova; Marina Ageeva; Natalia Gogoleva; Tatyana Gorshkova. Phloem fibres as motors of gravitropic behaviour of flax plants: level of transcriptome. Functional Plant Biology 2018, 45, 203 -214.

AMA Style

Oleg Gorshkov, Natalia Mokshina, Nadezda Ibragimova, Marina Ageeva, Natalia Gogoleva, Tatyana Gorshkova. Phloem fibres as motors of gravitropic behaviour of flax plants: level of transcriptome. Functional Plant Biology. 2018; 45 (2):203-214.

Chicago/Turabian Style

Oleg Gorshkov; Natalia Mokshina; Nadezda Ibragimova; Marina Ageeva; Natalia Gogoleva; Tatyana Gorshkova. 2018. "Phloem fibres as motors of gravitropic behaviour of flax plants: level of transcriptome." Functional Plant Biology 45, no. 2: 203-214.

Research article
Published: 01 January 2017 in Functional Plant Biology
Reads 0
Downloads 0

Cellulose synthesising complex consists of cellulose synthase (CESA) subunits encoded by a multigene family; different sets of CESA genes are known to be expressed during primary and secondary cell wall formation. We examined the expression of LusCESAs in flax (Linum usitatissimum L.) cellulosic fibres at various stages of development and in the course of graviresponse by means of RNA-Seq and quantitative PCR. Transcripts for both primary and secondary cell wall-related CESAs were abundant in fibres depositing highly cellulosic tertiary cell walls. Gravistimulation of flax plants temporally increased the abundance of CESA transcripts, specifically in phloem fibres located at the pulling stem side. Construction of coexpression networks for LusCESAs revealed that both primary and secondary cell wall-related CESAs were involved in the joint coexpression group in fibres depositing tertiary cell walls, as distinct from other tissues, where these genes were within separate groups. The obtained data suggest that fibres depositing tertiary cell walls have a specific mechanism of cellulose biosynthesis and a specific way of its regulation.

ACS Style

Natalia Mokshina; Oleg Gorshkov; Nadezda Ibragimova; Tatyana Chernova; Tatyana Gorshkova. Cellulosic fibres of flax recruit both primary and secondary cell wall cellulose synthases during deposition of thick tertiary cell walls and in the course of graviresponse. Functional Plant Biology 2017, 44, 820 .

AMA Style

Natalia Mokshina, Oleg Gorshkov, Nadezda Ibragimova, Tatyana Chernova, Tatyana Gorshkova. Cellulosic fibres of flax recruit both primary and secondary cell wall cellulose synthases during deposition of thick tertiary cell walls and in the course of graviresponse. Functional Plant Biology. 2017; 44 (8):820.

Chicago/Turabian Style

Natalia Mokshina; Oleg Gorshkov; Nadezda Ibragimova; Tatyana Chernova; Tatyana Gorshkova. 2017. "Cellulosic fibres of flax recruit both primary and secondary cell wall cellulose synthases during deposition of thick tertiary cell walls and in the course of graviresponse." Functional Plant Biology 44, no. 8: 820.

Article
Published: 15 December 2016 in Plant Molecular Biology
Reads 0
Downloads 0

Functional specialization of cells is among the most fundamental processes of higher organism ontogenesis. The major obstacle to studying this phenomenon in plants is the difficulty of isolating certain types of cells at defined stages of in planta development for in-depth analysis. A rare opportunity is given by the developed model system of flax (Linum usitatissimum L.) phloem fibres that can be purified from the surrounding tissues at the stage of the tertiary cell wall deposition. The performed comparison of the whole transcriptome profile in isolated fibres and other portions of the flax stem, together with fibre metabolism characterization, helped to elucidate the general picture of the advanced stage of plant cell specialization and to reveal novel participants potentially involved in fibre metabolism regulation and cell wall formation. Down-regulation of all genes encoding proteins involved in xylan and lignin synthesis and up-regulation of genes for the specific set of transcription factors transcribed during tertiary cell wall formation were revealed. The increased abundance of transcripts for several glycosyltransferases indicated the enzymes that may be involved in synthesis of fibre-specific version of rhamnogalacturonan I.

ACS Style

Oleg Gorshkov; Natalia Mokshina; Vladimir Gorshkov; Svetlana Chemikosova; Yuri Gogolev; Tatyana Gorshkova. Transcriptome portrait of cellulose-enriched flax fibres at advanced stage of specialization. Plant Molecular Biology 2016, 93, 431 -449.

AMA Style

Oleg Gorshkov, Natalia Mokshina, Vladimir Gorshkov, Svetlana Chemikosova, Yuri Gogolev, Tatyana Gorshkova. Transcriptome portrait of cellulose-enriched flax fibres at advanced stage of specialization. Plant Molecular Biology. 2016; 93 (4):431-449.

Chicago/Turabian Style

Oleg Gorshkov; Natalia Mokshina; Vladimir Gorshkov; Svetlana Chemikosova; Yuri Gogolev; Tatyana Gorshkova. 2016. "Transcriptome portrait of cellulose-enriched flax fibres at advanced stage of specialization." Plant Molecular Biology 93, no. 4: 431-449.

Journal article
Published: 22 January 2015 in Planta
Reads 0
Downloads 0

Specific α- l -arabinofuranosidases are involved in the realisation of elongation growth process in cells with type II cell walls. Elongation growth in a plant cell is largely based on modification of the cell wall. In type II cell walls, the Ara/Xyl ratio is known to decrease during elongation due to the partial removal of Ara residues from glucuronoarabinoxylan. We searched within the maize genome for the genes of all predicted α-L-arabinofuranosidases that may be responsible for such a process and related their expression to the activity of the enzyme and the amount of free arabinose measured in six zones of a growing maize root. Eight genes of the GH51 family (ZmaABFs) and one gene of the GH3 family (ZmaARA-I) were identified. The abundance of ZmaABF1 and 3-6 transcripts was highly correlated with the measured enzymatic activity and free arabinose content that significantly increased during elongation. The transcript abundances also coincided with the pattern of changes in the Ara/Xyl ratio of the xylanase-extractable glucuronoarabinoxylan described in previous studies. The expression of ZmaABF3, 5 and 6 was especially up-regulated during elongation although corresponding proteins are devoid of the catalytic glutamate at the proper position. ZmaABF2 transcripts were specifically enriched in the root cap and meristem. A single ZmaARA-I gene was not expressed as a whole gene but instead as splice variants that encode the C-terminal end of the protein. Changes in the ZmaARA-I transcript level were rather moderate and had no significant correlation with free arabinose content. Thus, elongation growth of cells with type II cell walls is accompanied by the up-regulation of specific and predicted α-L-arabinofuranosidase genes, and the corresponding activity is indeed pronounced and is important for the modification of glucuronoarabinoxylan, which plays a key role in the modification of the cell wall supramolecular organisation.

ACS Style

Liudmila Kozlova; Oleg V. Gorshkov; Natalia E. Mokshina; Tatyana A. Gorshkova. Differential expression of α-l-arabinofuranosidases during maize (Zea mays L.) root elongation. Planta 2015, 241, 1159 -1172.

AMA Style

Liudmila Kozlova, Oleg V. Gorshkov, Natalia E. Mokshina, Tatyana A. Gorshkova. Differential expression of α-l-arabinofuranosidases during maize (Zea mays L.) root elongation. Planta. 2015; 241 (5):1159-1172.

Chicago/Turabian Style

Liudmila Kozlova; Oleg V. Gorshkov; Natalia E. Mokshina; Tatyana A. Gorshkova. 2015. "Differential expression of α-l-arabinofuranosidases during maize (Zea mays L.) root elongation." Planta 241, no. 5: 1159-1172.

Journal article
Published: 01 May 2013 in Doklady Biochemistry and Biophysics
Reads 0
Downloads 0
ACS Style

Vladislav Chernov; Olga Chernova; O. V. Gorshkov; N. B. Baranova; A. A. Mouzykantov; T. N. Nesterova; A. A. Ponomareva. Interaction between mycoplasmas and plants: Extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii PG8. Doklady Biochemistry and Biophysics 2013, 450, 155 -159.

AMA Style

Vladislav Chernov, Olga Chernova, O. V. Gorshkov, N. B. Baranova, A. A. Mouzykantov, T. N. Nesterova, A. A. Ponomareva. Interaction between mycoplasmas and plants: Extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii PG8. Doklady Biochemistry and Biophysics. 2013; 450 (1):155-159.

Chicago/Turabian Style

Vladislav Chernov; Olga Chernova; O. V. Gorshkov; N. B. Baranova; A. A. Mouzykantov; T. N. Nesterova; A. A. Ponomareva. 2013. "Interaction between mycoplasmas and plants: Extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii PG8." Doklady Biochemistry and Biophysics 450, no. 1: 155-159.

English abstract
Published: 08 March 2012 in Молекулярная биология
Reads 0
Downloads 0
ACS Style

Vladislav Chernov; Olga Chernova; N B Baranova; Oleg Gorshkov; E S Medvedeva; G F Shaĭmardanova. [The adaptation of mycoplasmas to stress conditions: features of proteome shift in Mycoplasma hominis PG37 under starvation and low temperature]. Молекулярная биология 2012, 45, 1 .

AMA Style

Vladislav Chernov, Olga Chernova, N B Baranova, Oleg Gorshkov, E S Medvedeva, G F Shaĭmardanova. [The adaptation of mycoplasmas to stress conditions: features of proteome shift in Mycoplasma hominis PG37 under starvation and low temperature]. Молекулярная биология. 2012; 45 (5):1.

Chicago/Turabian Style

Vladislav Chernov; Olga Chernova; N B Baranova; Oleg Gorshkov; E S Medvedeva; G F Shaĭmardanova. 2012. "[The adaptation of mycoplasmas to stress conditions: features of proteome shift in Mycoplasma hominis PG37 under starvation and low temperature]." Молекулярная биология 45, no. 5: 1.

Journal article
Published: 01 January 2012 in The Scientific World Journal
Reads 0
Downloads 0

For the first time, the phytopathogenicity of extracellular vesicles ofAcholeplasma laidlawiiPG8 (a ubiquitous mycoplasma that is one of the five common species of cell culture contaminants and is a causative agent for phytomycoplasmoses) inOryza sativaL. plants was studied. Data on the ability of extracellular vesicles ofAcholeplasma laidlawiiPG8 to penetrate from the nutrient medium into overground parts ofOryza sativaL. through the root system and to cause alterations in ultrastructural organization of the plants were presented. As a result of the analysis of ultrathin leaf sections of plants grown in medium withA. laidlawiiPG8 vesicles, we detected significant changes in tissue ultrastructure characteristic to oxidative stress in plants as well as their cultivation along with bacterial cells. The presence of nucleotide sequences of some mycoplasma genes within extracellular vesicles ofAcholeplasma laidlawiiPG8 allowed a possibility to use PCR (with the following sequencing) to perform differential detection of cells and bacterial vesicles in samples under study. The obtained data may suggest the ability of extracellular vesicles of the mycoplasma to display in plants the features of infection from the viewpoint of virulence criteria—invasivity, infectivity—and toxigenicity—and to favor to bacterial phytopathogenicity.

ACS Style

Vladislav M. Chernov; Olga A. Chernova; Alexey A. Mouzykantov; Natalija B. Baranova; Oleg V. Gorshkov; Maxim V. Trushin; Tatiana N. Nesterova; Anastasia A. Ponomareva. Extracellular Membrane Vesicles and Phytopathogenicity ofAcholeplasma laidlawiiPG8. The Scientific World Journal 2012, 2012, 1 -6.

AMA Style

Vladislav M. Chernov, Olga A. Chernova, Alexey A. Mouzykantov, Natalija B. Baranova, Oleg V. Gorshkov, Maxim V. Trushin, Tatiana N. Nesterova, Anastasia A. Ponomareva. Extracellular Membrane Vesicles and Phytopathogenicity ofAcholeplasma laidlawiiPG8. The Scientific World Journal. 2012; 2012 ():1-6.

Chicago/Turabian Style

Vladislav M. Chernov; Olga A. Chernova; Alexey A. Mouzykantov; Natalija B. Baranova; Oleg V. Gorshkov; Maxim V. Trushin; Tatiana N. Nesterova; Anastasia A. Ponomareva. 2012. "Extracellular Membrane Vesicles and Phytopathogenicity ofAcholeplasma laidlawiiPG8." The Scientific World Journal 2012, no. : 1-6.

Journal article
Published: 18 November 2011 in Journal of Proteomics
Reads 0
Downloads 0

For the first time, we studied the phytopathogenicity toward Oryza sativa L. of unadapted and adapted to unfavorable environment (starvation) cells of Acholeplasma laidlawii PG8--ubiquitous mycoplasma found in the soil, waste waters, tissues of the highest eukaryotes and being the basic contaminant of cell cultures and a causative agent of phytomycoplasmoses. The features of morphology, ultrastructural organization and proteomes of unadapted and adapted cells of the mycoplasma and infected plants were presented. Using 2D-DIGE and MS, 43 proteins of O. sativa L. that were differentially expressed in the leaves of plants cultivated in media with A. laidlawii PG8 were identified. The qualitative and quantitative responses of the plant proteome toward adapted and unadapted mycoplasma cells differed. That may be explained by differences in the virulence of the corresponding bacterial cells. Using 2D-DIGE and MS, 82 proteins that were differentially expressed in adapted and unadapted mycoplasma cells were detected. In adapted cells of the mycoplasma, in comparison with unadapted ones, a significant increase in the expression of PNPase--a global regulator of virulence in phytopathogenic bacteria occurred; there was also decreased expression of 40 proteins including 14 involved in bacterial virulence and the expression of 31 proteins including 5 involved in virulence was not detected. We propose that differences in the phytopathogenicity of adapted and unadapted A. laidlawii PG8 cells may be related to features of their proteomes and membrane vesicles.

ACS Style

Vladislav M. Chernov; Olga Chernova; Elena S. Medvedeva; Alexey A. Mouzykantov; Anastasia A. Ponomareva; Gulnara F. Shaymardanova; Oleg Gorshkov; Maxim V. Trushin. Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis. Journal of Proteomics 2011, 74, 2920 -2936.

AMA Style

Vladislav M. Chernov, Olga Chernova, Elena S. Medvedeva, Alexey A. Mouzykantov, Anastasia A. Ponomareva, Gulnara F. Shaymardanova, Oleg Gorshkov, Maxim V. Trushin. Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis. Journal of Proteomics. 2011; 74 (12):2920-2936.

Chicago/Turabian Style

Vladislav M. Chernov; Olga Chernova; Elena S. Medvedeva; Alexey A. Mouzykantov; Anastasia A. Ponomareva; Gulnara F. Shaymardanova; Oleg Gorshkov; Maxim V. Trushin. 2011. "Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis." Journal of Proteomics 74, no. 12: 2920-2936.

Journal article
Published: 08 October 2011 in Molecular Biology
Reads 0
Downloads 0

Mycoplasma hominis is a widely spread mycoplasma (class Mollicutes), associated with socially important human diseases and contamination of cell cultures. Controlling infections caused by M. hominis depends on determining the molecular mechanisms responsible for the bacterium’s survival in unfavorable conditions. A proteome analysis employing 2-DIGE and MALDI TOF/TOF MS was applied to identify, for the first time, 53 proteins of M. hominis PG37 whose levels altered in bacteria cultivated in stress conditions (starvation and low temperature). According to the protein classification by functional category (clusters of orthologous groups of proteins, COG), 47 of the 53 mycoplasma proteins identified are involved in fundamental cellular and biochemical processes: translation (12; 22.64%), transcription (2; 3.77%), posttranslational modification (7; 13.20%), cell cycle control (2; 3.77%), energy production and conversion (6; 11.32%), carbohydrate transport and metabolism (3; 5.66%), amino acid transport and metabolism (8; 15.09%), nucleotide transport and metabolism (6; 11.32%), and inorganic ion transport and metabolism (1; 1.89%). For six proteins (11.32%), the function was not determined; 24 proteins (45.28%) were bacterial virulence factors. Those proteins of M. hominis PG37 whose expression is modulated in response to unfavorable environmental conditions are components of stress adaptation mechanisms in mycoplasma and potential targets for controlling infections caused by this bacterium.

ACS Style

V. M. Chernov; Olga Chernova; N. B. Baranova; Oleg Gorshkov; E. S. Medvedeva; G. F. Shaymardanova. Mycoplasma adaptation to stress conditions: Proteome shift in Mycoplasma hominis PG37 in response to starvation and low temperatures. Molecular Biology 2011, 45, 843 -851.

AMA Style

V. M. Chernov, Olga Chernova, N. B. Baranova, Oleg Gorshkov, E. S. Medvedeva, G. F. Shaymardanova. Mycoplasma adaptation to stress conditions: Proteome shift in Mycoplasma hominis PG37 in response to starvation and low temperatures. Molecular Biology. 2011; 45 (5):843-851.

Chicago/Turabian Style

V. M. Chernov; Olga Chernova; N. B. Baranova; Oleg Gorshkov; E. S. Medvedeva; G. F. Shaymardanova. 2011. "Mycoplasma adaptation to stress conditions: Proteome shift in Mycoplasma hominis PG37 in response to starvation and low temperatures." Molecular Biology 45, no. 5: 843-851.

Journal article
Published: 24 May 2011 in Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry
Reads 0
Downloads 0

The role of transition metal ions in atherogenesis is controversial; they may be involved in hydroxyl radical generation and can also catalyze the reactive oxygen species neutralization reaction as cofactors of antioxidant enzymes. Using EPR spectroscopy, we revealed that 70% of aorta specimens with atherosclerotic lesions possessed superoxide dismutase activity, 100% of the specimens initiated Fenton reaction and demonstrated the presence of manganese paramagnetic centers. The sodA gene encoding manganese-dependent bacterial superoxide dismutase was not found in the samples of atherosclerotic plaques by PCR using degenerate primers. The data obtained indicate prospects of manganese analysis as a marker element in the express diagnostics of atherosclerosis.

ACS Style

A. P. Lozhkin; T. B. Biktagirov; V. A. Abdul’Yanov; O. V. Gorshkov; E. V. Timonina; G. V. Mamin; S. B. Orlinskii; N. I. Silkin; Vladislav Chernov; R. N. Khairullin; M. Kh. Salakhov; O. N. Ilinskaya. Manganese in atherogenesis: Detection, origin, and a role. Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry 2011, 5, 158 -162.

AMA Style

A. P. Lozhkin, T. B. Biktagirov, V. A. Abdul’Yanov, O. V. Gorshkov, E. V. Timonina, G. V. Mamin, S. B. Orlinskii, N. I. Silkin, Vladislav Chernov, R. N. Khairullin, M. Kh. Salakhov, O. N. Ilinskaya. Manganese in atherogenesis: Detection, origin, and a role. Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry. 2011; 5 (2):158-162.

Chicago/Turabian Style

A. P. Lozhkin; T. B. Biktagirov; V. A. Abdul’Yanov; O. V. Gorshkov; E. V. Timonina; G. V. Mamin; S. B. Orlinskii; N. I. Silkin; Vladislav Chernov; R. N. Khairullin; M. Kh. Salakhov; O. N. Ilinskaya. 2011. "Manganese in atherogenesis: Detection, origin, and a role." Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry 5, no. 2: 158-162.

Short communication
Published: 30 May 2010 in Microbiological Research
Reads 0
Downloads 0

The data obtained in this study proved that Mycoplasma gallisepticum S6 known as avian pathogen had a phytopathogenic potential. The vegetative forms and the viable but nonculturable (VBNC) forms of this mycoplasma could infect the plants via an assemblage of rootlets, invade different tissues, persist there and cause destructive events characteristic to phytomycoplasmoses. In comparison with the vegetative forms, the VBNC forms induced more prominent destructive changes. This phenomenon might be connected to increasing expression of proteins responsible for virulence in the bacterial cells. The fact that M. gallisepticum S6 could demonstrate virulent features (infectivity, invasiveness, persistence and toxigenicity) in regard to plants seems to require a development of new ways for controlling phytomycoplasmoses taking into account the probable presence of asymptomatic carriers of this bacterium.

ACS Style

Vladislav Chernov; Olga Chernova; Alexey A. Mouzykantov; Anastasia A. Ponomareva; Maxim V. Trushin; Oleg V. Gorshkov; Tatyana N. Nesterova. Phytopathogenicity of avian mycoplasma Mycoplasma gallisepticum S6: Morphologic and ultracytostructural changes in plants infected with the vegetative forms and the viable but nonculturable forms of the bacterium. Microbiological Research 2010, 165, 346 -350.

AMA Style

Vladislav Chernov, Olga Chernova, Alexey A. Mouzykantov, Anastasia A. Ponomareva, Maxim V. Trushin, Oleg V. Gorshkov, Tatyana N. Nesterova. Phytopathogenicity of avian mycoplasma Mycoplasma gallisepticum S6: Morphologic and ultracytostructural changes in plants infected with the vegetative forms and the viable but nonculturable forms of the bacterium. Microbiological Research. 2010; 165 (4):346-350.

Chicago/Turabian Style

Vladislav Chernov; Olga Chernova; Alexey A. Mouzykantov; Anastasia A. Ponomareva; Maxim V. Trushin; Oleg V. Gorshkov; Tatyana N. Nesterova. 2010. "Phytopathogenicity of avian mycoplasma Mycoplasma gallisepticum S6: Morphologic and ultracytostructural changes in plants infected with the vegetative forms and the viable but nonculturable forms of the bacterium." Microbiological Research 165, no. 4: 346-350.

Journal article
Published: 01 January 2010 in The Scientific World Journal
Reads 0
Downloads 0

This article reports on a study of some characteristics of DNA extracted from the vegetative and viable, but nonculturable (VBNC), cells of two mycoplasma species (Acholeplasma laidlawiiPG8 andMycoplasma hominisPG37) using atomic force microscopy (AFM). DNA images were obtained by operating the AFM microscope in the tapping mode. It was found that DNA from the VBNC forms ofM. hominisPG37 has decreased sizes (height: 0.177 ± 0.026 nm vs. 0.391 ± 0.041 nm for the vegetative forms, and width: 1.92 ± 0.099 vs. 2.17 ± 0.156 nm for the vegetative forms) in comparison to DNA from the vegetative forms of the mycoplasma. In the case of DNA from theA. laidlawiiPG8 VBNC forms, we detected a decrease in width (1.506 ± 0.076 nm vs. 1.898 ± 0.117 nm for the vegetative forms), but an increase in height (0.641 ± 0.068 nm vs. 0.255 ± 0.010 nm for the vegetative forms) of the molecule. Analyzing the obtained results, one can speculate on some similarities in the physical-chemical properties of DNA fromM. hominisPG37 andM. gallisepticumS6. In turn, this implies some general mechanisms of adaptation to a severe environment.

ACS Style

Maxim V. Trushin; Vladislav M. Chernov; Oleg V. Gorshkov; Natalija B. Baranova; Olga A. Chernova. Atomic Force Microscopy Analysis of DNA Extracted from the Vegetative Cells and the Viable, but Nonculturable, Cells of Two Mycoplasmas (Acholeplasma laidlawiiPG8 andMycoplasma hominisPG37). The Scientific World Journal 2010, 10, 894 -900.

AMA Style

Maxim V. Trushin, Vladislav M. Chernov, Oleg V. Gorshkov, Natalija B. Baranova, Olga A. Chernova. Atomic Force Microscopy Analysis of DNA Extracted from the Vegetative Cells and the Viable, but Nonculturable, Cells of Two Mycoplasmas (Acholeplasma laidlawiiPG8 andMycoplasma hominisPG37). The Scientific World Journal. 2010; 10 ():894-900.

Chicago/Turabian Style

Maxim V. Trushin; Vladislav M. Chernov; Oleg V. Gorshkov; Natalija B. Baranova; Olga A. Chernova. 2010. "Atomic Force Microscopy Analysis of DNA Extracted from the Vegetative Cells and the Viable, but Nonculturable, Cells of Two Mycoplasmas (Acholeplasma laidlawiiPG8 andMycoplasma hominisPG37)." The Scientific World Journal 10, no. : 894-900.

Journal article
Published: 20 October 2009 in Doklady Biochemistry and Biophysics
Reads 0
Downloads 0
ACS Style

Vladislav Chernov; Olga Chernova; A. A. Mouzykantov; O. V. Gorshkov; T. N. Nesterova; A. A. Ponomareva; I. A. Demina; M. V. Serebryakova; V. M. Govorun. Adaptation of mycoplasmas to adverse environments: Phytopathogenicity and peculiarities of protein expression of vegetative and nonculturable forms of Mycoplasma gallisepticum S6 cells. Doklady Biochemistry and Biophysics 2009, 428, 273 -276.

AMA Style

Vladislav Chernov, Olga Chernova, A. A. Mouzykantov, O. V. Gorshkov, T. N. Nesterova, A. A. Ponomareva, I. A. Demina, M. V. Serebryakova, V. M. Govorun. Adaptation of mycoplasmas to adverse environments: Phytopathogenicity and peculiarities of protein expression of vegetative and nonculturable forms of Mycoplasma gallisepticum S6 cells. Doklady Biochemistry and Biophysics. 2009; 428 (1):273-276.

Chicago/Turabian Style

Vladislav Chernov; Olga Chernova; A. A. Mouzykantov; O. V. Gorshkov; T. N. Nesterova; A. A. Ponomareva; I. A. Demina; M. V. Serebryakova; V. M. Govorun. 2009. "Adaptation of mycoplasmas to adverse environments: Phytopathogenicity and peculiarities of protein expression of vegetative and nonculturable forms of Mycoplasma gallisepticum S6 cells." Doklady Biochemistry and Biophysics 428, no. 1: 273-276.

English abstract
Published: 08 October 2009 in Молекулярная биология
Reads 0
Downloads 0
ACS Style

Vladislav Chernov; Olga Chernova; Oleg Gorshkov; A A Muzykantov. [Adaptation of mycoplasmas to stressful factors: nucleotide sequences nonregistered in vegetative forms of M. galisepticum S6 cells are revealed in nonculturable forms of the mycoplasma]. Молекулярная биология 2009, 43, 1 .

AMA Style

Vladislav Chernov, Olga Chernova, Oleg Gorshkov, A A Muzykantov. [Adaptation of mycoplasmas to stressful factors: nucleotide sequences nonregistered in vegetative forms of M. galisepticum S6 cells are revealed in nonculturable forms of the mycoplasma]. Молекулярная биология. 2009; 43 (4):1.

Chicago/Turabian Style

Vladislav Chernov; Olga Chernova; Oleg Gorshkov; A A Muzykantov. 2009. "[Adaptation of mycoplasmas to stressful factors: nucleotide sequences nonregistered in vegetative forms of M. galisepticum S6 cells are revealed in nonculturable forms of the mycoplasma]." Молекулярная биология 43, no. 4: 1.