Here, we report the complete genome sequence of Bacteroidales bacterium strain MB20-C3-3 (=BCRC 81427) isolated from sewage sludge. The genome is 2,379,874 bp in length with a guanine and cytosine content of 40.0%. The genome of strain MB20-C3-3 was used for further species delineation and comparative genomic analyses.

To date, only a few microbial community studies of cold seeps at the South China Sea (SCS) have been reported. The cold seep dominated by tubeworms was discovered at South Yungan East Ridge (SYER) offshore southwestern Taiwan by miniROV. The tubeworms were identified and proposed as Paraescarpia formosa sp. nov. through morphological and phylogenetic analyses. The endosymbionts in the trunk of P. formosa analyzed by a 16S rRNA gene clone library represented only one phylotype, which belonged to the family Sedimenticolaceae in Gammaproteobacteria. In addition, the archaeal and bacterial communities in the habitat of tubeworm P. formosa were investigated by using high-phylogenetic-resolution full-length 16S rRNA gene amplicon sequencing. The results showed that anerobic methane-oxidizing archaea (ANME)-1b was most abundant and ANME-2ab was minor in a consortia of the anerobic oxidation of methane (AOM). The known sulfate-reducing bacteria (SRB) partners in AOM consortia, such as SEEP-SRB1, -SRB2, and -SRB4, Desulfococcus and Desulfobulbus, occurred in a small population (0–5.7%) at the SYER cold seep, and it was suggested that ANME-1b and ANME-2ab might be coupled with multiple SRB in AOM consortia. Besides AOM consortia, various methanogenic archaea, including Bathyarchaeota (Subgroup-8), Methanocellales, Methanomicrobiales, Methanosarcinales, Methanofastidiosales and Methanomassiliicoccales, were identified, and sulfur-oxidizing bacteria Sulfurovum and Sulfurimonas in phylum Epsilonbacteraeota were dominant. This study revealed the first investigation of microbiota in and around tubeworm P. formosa discovered at the SYER cold seep offshore southwestern Taiwan. We could gain insights into the chemosynthetic communities in the deep sea, especially regarding the cold seep ecosystems at the SCS.

Here, we report the complete genome sequence of Oscillospiraceae bacterium MB08-C2-2 (=BCRC 81394), isolated from feces of black soldier fly ( Hermetia illucens ) larvae, comprising 3,292,498 bp, a GC content of 49.67%, and identification of a 72,574 bp plasmid for further species delineation and comparative genomic analysis.

Here, we report the complete genome sequence of Anaerocolumna sp. MB42-C2 isolated from a hot spring in Qingliu, Fujian, China. The genome comprises 6,437,239 bp with a G + C content of 36.49 mol% and provides a genomic basis for further species delineation and characterization of genomic features.

Here, we report the complete genome sequence of Tissierella sp. strain MB52-C2 (= BCRC 81439) isolated from Wuyi Mountain soil, the source region of Wuyi rock tea. The genome is 3,892,263 bp with 30.9% G + C content and supports species delineation and genomic analyses of taxonomic position and genomic features.

Here, we report the complete genome sequence of Actinomycetaceae bacterium strain MB13-C1-2, isolated from the feces of black soldier fly ( Hermetia illucens ) larvae, comprising 2,578,609 bp and a GC content of 57.23%. The genome was selected for further analyses of species delineation and genomic features.