The development of innovative active food packaging is a promising strategy to mitigate food loss and waste while enhancing food safety, extending shelf life, and maintaining overall quality. In this review, Pickering emulsions with essential oils are critically evaluated as active additives for sustainable food packaging films, focusing on their antimicrobial and antioxidant properties, stabilization mechanisms, and physicochemical performances. A bibliometric approach was used to contextualize the current research landscape and new trends. Data were collected from the Web of Science and Scopus databases to find studies published between 2020 and 2024. The analysis of 51 articles shows that cinnamon, clove, and oregano are the most used essential oils, while cellulose and chitosan are the predominant polymer matrices. Pickering emulsions as stabilizers in food science represent a step forward in sustainable emulsion technology. The incorporation of essential oils into biobased films via Pickering emulsions can improve the mechanical and barrier properties, antimicrobial and antioxidant activities, and shelf life of foods. This approach offers a natural, environmentally friendly alternative to conventional materials and is in line with the 2030 Agenda goals for sustainability and responsible consumption. Recent advances show that composite particles combining polysaccharides and proteins have higher stability and functionality compared to single particles due to their optimized interactions at the interfaces. Future research should focus on developing scalable, cost-effective production methods and conducting comprehensive environmental testing and regulatory compliance, particularly for nanotechnology-based packaging. These efforts will be crucial to drive the development of safe and effective biobased active food packaging.

The Mediterranean area is one of the major global producers of agricultural food. However, along the entire supply chain—from farming to food distribution and consumption—food waste represents a significant fraction. Additionally, plant waste residues generated during the cultivation of specific fruits and vegetables must also be considered. This heterogeneous biomass is a valuable source of bioactive compounds and materials that can be transformed into high-performance functional products. By analyzing technical and scientific literature, this review identifies extraction, composite production, and bioconversion as the main strategies for valorizing agricultural by-products and waste. The advantages of these approaches as well as efficiency gains through digitalization are discussed, along with their potential applications in the Mediterranean region to support new research activities and bioeconomic initiatives. Moreover, the review highlights the challenges and disadvantages associated with waste valorization, providing a critical comparison of different studies to offer a comprehensive perspective on the topic. The objective of this review is to evaluate the potential of agricultural waste valorization, identifying effective strategies while also considering their limitations, to contribute to the development of sustainable and innovative solutions in Mediterranean bioeconomy.

Masson Pine pollen (Pinus massoniana; MP) are used in Traditional Chinese Medicine to treat gut conditions. Early in vivo work supports this claim and suggests interaction of the material with the gastrointestinal immune system. The present study tested if and how MP material activates HD11 chicken macrophages in vitro using material from different production sites and harvest years. We applied twelve batches of MP from different Chinese production sites and harvest years. Materials were subjected to LAL tests (endotoxic activity), GC-MS (fatty acid analysis), and plate techniques (microbiological background, antimicrobial activity). Furthermore, HD11 chicken macrophages were challenged (6 h, 37 °C) with MP or LPS (E. coli O111:B4), respectively, to quantify nitric oxide (NO) production and immune gene expression (RT-qPCR). MP material promoted strong signals in LAL tests and contained significant amounts of 3-hydroxydodecanoic acid and 3-hydroxymyristic acid, irrespective of processing, harvest year, or origin. The pollen material activated HD11 chicken macrophages, which was confirmed by spikes of NO release and k-means cluster analysis of TLR-signaling pathway gene expression data. Response of NO production to Log₂-titration of MP and LPS-treated media was in any case linear and significant. The response was reduced by polymyxin-B (PMB) and the inhibition was twice as strong for LPS than MP. No or minor microbiological background was detected on the majority of MP samples. Three samples showed presence of spoilage microorganisms and Gram-negative bacteria, but this did not correlate to LAL data or bacterial DNA counts. No antimicrobial activity of MP was evident. Pollen of the Masson Pine activated HD11 chicken macrophages in vitro, which is likely partially due to a background of bacterial LPS associated with the pollen material. However, as most of the effect (appr. 80%) could not be blocked by PMB this is certainly due to other stimuli. We hypothesize that polysaccharides and oligosaccharides of the pollen matrix have the potential to interact with certain immune receptors presented on the plasma membrane of chicken macrophages.